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Full-Text Articles in Medicine and Health Sciences
Structure Of Shiga Toxin Type 2 (Stx2) From Escherichia Coli O157:H7, Marie E. Fraser, Masao Fujinaga, Maia M. Cherney, Angela R. Melton-Celsa, Edda M. Twiddy, Alison D. O'Brien, Michael N. G. James
Structure Of Shiga Toxin Type 2 (Stx2) From Escherichia Coli O157:H7, Marie E. Fraser, Masao Fujinaga, Maia M. Cherney, Angela R. Melton-Celsa, Edda M. Twiddy, Alison D. O'Brien, Michael N. G. James
Uniformed Services University of the Health Sciences
Several serotypes of Escherichia coli produce protein toxins closely related to Shiga toxin (Stx) from Shigella dysenteriae serotype 1. These Stx-producing E. coli cause outbreaks of hemorrhagic colitis and hemolytic uremic syndrome in humans, with the latter being more likely if the E. coli produce Stx2 than if they only produce Stx1. To investigate the differences among the Stxs, which are all AB5 toxins, the crystal structure of Stx2 from E. coli O157:H7 was determined at 1.8-Å resolution and compared with the known structure of Stx. Our major finding was that, in contrast to Stx, the active site of …
Using Lc-Ms With De Novo Software To Fully Characterize The Multiple Methylations Of Lysine Residues In A Recombinant Fragment Of An Outer Membrane Protein From A Virulent Strain Of Rickettsia Prowazekii, Chien-Chung Chao, Shiaw-Lin Wu, Wei-Mei Ching
Using Lc-Ms With De Novo Software To Fully Characterize The Multiple Methylations Of Lysine Residues In A Recombinant Fragment Of An Outer Membrane Protein From A Virulent Strain Of Rickettsia Prowazekii, Chien-Chung Chao, Shiaw-Lin Wu, Wei-Mei Ching
Uniformed Services University of the Health Sciences
The outer membrane protein B (OmpB) of the typhus group rickettsiae is an immunodominant antigen and has been shown to provide protection against typhus in animal models. Consequently, OmpB is currently being considered as a potential rickettsiae vaccine candidate to be used in humans. The OmpB from virulent strains are heavily methylated while the attenuated strains are hypomethylated. Western blot analysis of partially digested OmpB revealed that one of the reactive fragments was located at the N-terminus (fragment A, aa 33–272). Recently, we have over expressed, purified, and chemically methylated the recombinant fragment A from Rickettsia prowazekii (Ap). The methylated …
Cognitive-Behavioral Therapy, Light Therapy, And Their Combination In Treating Seasonal Affective Disorder, Kelly J. Rohan, Kathryn Tierney Lindsey, Katheryn A. Roecklein, Timothy J. Lacy
Cognitive-Behavioral Therapy, Light Therapy, And Their Combination In Treating Seasonal Affective Disorder, Kelly J. Rohan, Kathryn Tierney Lindsey, Katheryn A. Roecklein, Timothy J. Lacy
Uniformed Services University of the Health Sciences
Background: The need to develop supplementary or alternative treatments for seasonal affective disorder (SAD) is underscored by the significant minority (47%) of SAD patients that is refractory to light therapy, the persistence of residual symptoms despite light treatment, and poor long-term compliance with light use. Because preliminary studies suggest that cognitive and behavioral factors are involved in SAD, cognitive-behavioral therapy (CBT) warrants investigation as a possible treatment option. Methods: We piloted a 6-week randomized clinical trial to compare a standard light therapy protocol; a novel, SAD-tailored, group CBT intervention; and their combination in ameliorating and remitting a current SAD episode …
Functional Characterization Of The Arabidopsis Thaliana Orthologue Of Tsc13p, The Enoyl Reductase Of The Yeast Microsomal Fatty Acid Elongating System, Kenneth Gable, Sarah Garton, Johnathan A. Napier, Teresa M. Dunn
Functional Characterization Of The Arabidopsis Thaliana Orthologue Of Tsc13p, The Enoyl Reductase Of The Yeast Microsomal Fatty Acid Elongating System, Kenneth Gable, Sarah Garton, Johnathan A. Napier, Teresa M. Dunn
Uniformed Services University of the Health Sciences
The protein encoded by the Arabidopsis At3g55360 gene was selected as a candidate for the enoyl reductase of the microsomal elongase system based on its homology to the Tsc13p protein of S. cerevisiae. The studies presented here demonstrate that heterologous expression of At3g55360 functionally complements the temperature-sensitive phenotype of a yeast tsc13 mutant that is deficient in enoyl reductase activity. Furthermore, AtTSC13 is shown to interact physically with the Elo2p and Elo3p components of the yeast elongase complex. At3g55360 apparently encodes the sole enoyl reductase activity associated with microsomal fatty acid elongation in Arabidopsis. Consistent with this conclusion, …
Yeast Genome-Wide Drug-Induced Haploinsufficiency Screen To Determine Drug Mode Of Action, Kristin Baetz, Lianne Mchardy, Ken Gable, Tamsin Tarling, Delphine Rebérioux, Jenny Bryan, Raymond J. Andersen, Teresa Dunn, Phil Hieter, Michel Roberge
Yeast Genome-Wide Drug-Induced Haploinsufficiency Screen To Determine Drug Mode Of Action, Kristin Baetz, Lianne Mchardy, Ken Gable, Tamsin Tarling, Delphine Rebérioux, Jenny Bryan, Raymond J. Andersen, Teresa Dunn, Phil Hieter, Michel Roberge
Uniformed Services University of the Health Sciences
Published in PNAS (2004) 101(13): pp. 4525-4530; doi:10.1073/pnas.0307122101
Plant Cell-Based Intimin Vaccine Given Orally To Mice Primed With Intimin Reduces Time Of Escherichia Coli O157:H7 Shedding In Feces, Nicole A. Judge, Hugh S. Mason, Alison D. O'Brien
Plant Cell-Based Intimin Vaccine Given Orally To Mice Primed With Intimin Reduces Time Of Escherichia Coli O157:H7 Shedding In Feces, Nicole A. Judge, Hugh S. Mason, Alison D. O'Brien
Uniformed Services University of the Health Sciences
Intimin is the primary adhesin of Escherichia coli O157:H7, the most common infectious cause of bloody diarrhea in the United States and the leading cause of acute kidney failure in children who develop hemolytic uremic syndrome. Cattle are the primary reservoir of E. coli O157:H7. Indeed, most cases of E. coli O157:H7 infection in the United States occur after ingestion of contaminated undercooked hamburger or produce that had contact with bovine manure. Because intimin is required for persistent colonization of neonatal calves and adult cattle, we hypothesized that an intimin-based vaccination strategy in calves would reduce colonization of cattle with …