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Tsc3p Is An 80-Amino Acid Protein Associated With Serine Palmitoyltransferase And Required For Optimal Enzyme Activity, Ken Gable, Harry Slife, Dagmar Bacikova, Erin Monaghan, Teresa M. Dunn
Tsc3p Is An 80-Amino Acid Protein Associated With Serine Palmitoyltransferase And Required For Optimal Enzyme Activity, Ken Gable, Harry Slife, Dagmar Bacikova, Erin Monaghan, Teresa M. Dunn
Uniformed Services University of the Health Sciences
Serine palmitoyltransferase catalyzes the first step of sphingolipid synthesis, condensation of serine and palmitoyl CoA to form the long chain base 3-ketosphinganine. The LCB1/TSC2 and LCB2/TSC1 genes encode homologous proteins of the a-oxoamine synthase family required for serine palmitoyltransferase activity. The other a-oxoamine synthases are soluble homodimers, but serine palmitoyltransferase is a membrane-associated enzyme composed of at least two subunits, Lcb1p and Lcb2p. Here, we report the characterization of a third gene, TSC3, required for optimal 3-ketosphinganine synthesis in Saccharomyces cerevisiae. S. cerevisiae cells lacking the TSC3 gene have a temperature-sensitive lethal phenotype that is reversed by supplying …
Escherichia Coli O157:H7 In Beef Cattle Presented For Slaughter In The U.S.: Higher Prevalence Rates Than Previously Estimated, Lisa J. Gansheroff, Alison D. O'Brien
Escherichia Coli O157:H7 In Beef Cattle Presented For Slaughter In The U.S.: Higher Prevalence Rates Than Previously Estimated, Lisa J. Gansheroff, Alison D. O'Brien
Uniformed Services University of the Health Sciences
Before 1982, the Centers for Disease Control and Prevention (CDC) had identified only one Escherichia coli isolate of the O157:H7 serotype; it had been isolated from a patient with bloody diarrhea. Then, in 1982, two outbreaks of severe bloody diarrhea occurred among people who had eaten hamburgers at a fast food chain. The CDC isolated E. coli O157:H7 from people who had become ill as well as from a hamburger patty. In 1983, Karmali et al. discovered an association between infection with E. coli that produce Shiga toxin (then called Vero toxin), including O157:H7 strains, and another severe and sometimes …