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Full-Text Articles in Medicine and Health Sciences
Detection Of The Eaea Gene In Escherichia Coli From Chickens By Polymerase Chain Reaction, Ayşe Kiliç, Hasan Basri̇ Ertaş, Adi̇le Muz, Gökben Özbey, Hakan Kalender
Detection Of The Eaea Gene In Escherichia Coli From Chickens By Polymerase Chain Reaction, Ayşe Kiliç, Hasan Basri̇ Ertaş, Adi̇le Muz, Gökben Özbey, Hakan Kalender
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in E. coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. E. coli was isolated from 48% (48/100) of the samples. DNA was extracted from these isolates and was amplified by PCR, using a pair of primers derived from the eaeA (virulence) gene. In the agarose gel examination of PCR products, 48% (48/100) of the isolates were determined …
Characterization Of Footrot Bacteria Dichelobacter Nodosus Using Pcr Amplification And Dna Sequence Analysis, Ifakat Tülay Çağatay, Jon G. H. Hickford
Characterization Of Footrot Bacteria Dichelobacter Nodosus Using Pcr Amplification And Dna Sequence Analysis, Ifakat Tülay Çağatay, Jon G. H. Hickford
Turkish Journal of Veterinary & Animal Sciences
Dichelobacter nodosus is an essential causative agent of footrot in ruminants, particularly in sheep, goats and cattle. In this study, more than 100 footrot samples were collected from 4 different farming regions in New Zealand (NZ). Selective media were chosen and isolation and routine growth conditions were optimized for NZ D. nodosus serotypes. Approximately 1000 primary plates were anaerobically subcultured several times and examined with Gram staining in order to detect single colonies of D. nodosus. Both the variable region and a part of the conserved region of fimbrial subunit gene (fimA) were amplified from bacterial DNA using polymerase chain …
Comparison Of Polymerase Chain Reaction And Conventional Methods For The Diagnosis Of Listeria Monocytogenes In Stuffed Mussels, Ergün Ö. Göksoy, Şükrü Kirkan, Osman Kaya
Comparison Of Polymerase Chain Reaction And Conventional Methods For The Diagnosis Of Listeria Monocytogenes In Stuffed Mussels, Ergün Ö. Göksoy, Şükrü Kirkan, Osman Kaya
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate the role of stuffed mussel as a source of Listeria monocytogenes. Polymerase chain reaction is a rapid procedure with both sensitivity and specificity for quick detection and identification of L. monocytogenes. A total of 50 mussel samples were investigated for L. monocytogenes. L. monocytogenes was not identified by the conventional method. However, PCR amplification products demonstrated that 5 out of 50 samples showed positive reactions with L. monocytogenes. The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.
Identification Of Vibrio Anguillarum By Pcr (Rpon Gene) Associated With Vibriosis In Marine Fish In Turkey, Di̇dem Demi̇rcan, Akin Candan
Identification Of Vibrio Anguillarum By Pcr (Rpon Gene) Associated With Vibriosis In Marine Fish In Turkey, Di̇dem Demi̇rcan, Akin Candan
Turkish Journal of Veterinary & Animal Sciences
The main causative agent of vibriosis is Vibrio anguillarum. In this study, 33 V. anguillarum strains were isolated from the internal organs of marine fish showing typical clinical signs of vibriosis, and these strains were identified by biochemical tests. For comparison of these results, API 20E and BIONOR Mono-Va agglutination kits were used. Finally strains were amplified by PCR using the rpoN gene. Although the phenotypic properties of V. anguillarum strains varied, the rpoN gene from chromosomal DNA was amplified in all strains. In conclusion, this gene could be used in the diagnosis of V. anguillarum strains isolated in Turkey.
Detection Of Listeria Monocytogenes By Using Pcr In Helix Pomatia, Şükrü Kirkan, Ergün Ö. Göksoy, Osman Kaya
Detection Of Listeria Monocytogenes By Using Pcr In Helix Pomatia, Şükrü Kirkan, Ergün Ö. Göksoy, Osman Kaya
Turkish Journal of Veterinary & Animal Sciences
The detection of Listeria monocytogenes in Helix pomatia, both raw and cooked is presented here. Polymerase chain reaction (PCR) is a rapid procedure with both sensitivity and specificity for quick detection and identification of Listeria monocytogenes from raw and cooked Helix pomatia. A total of 30 bags (10 g each) of H. pomatia samples were investigated for the Listeria monocytogenes inlB gene with the PCR method. PCR amplification products demonstrated that 18 of the 30 samples showed positive reactions to Listeria monocytogenes in the PCR. All PCR positive samples showed specific amplification of the 343 bp fragment for Listeria monocytogenes.
Isolation Of Clostridium Perfringens From Chickens And Detection Of The Alpha Toxin Gene By Polymerase Chain Reaction (Pcr), Hakan Kalender, Hasan Basri̇ Ertaş
Isolation Of Clostridium Perfringens From Chickens And Detection Of The Alpha Toxin Gene By Polymerase Chain Reaction (Pcr), Hakan Kalender, Hasan Basri̇ Ertaş
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to isolate Clostridium perfringens from chickens and to detect the gene encoding the alpha toxin produced by all types of C. perfringens by polymerase chain reaction (PCR). Intestinal contents of 160 slaughtered chickens from 8 different farms in Elazığ province were analyzed. C. perfringens was isolated from 8 (5%) of the samples. DNA samples extracted from suspected isolates grown on selective agar were amplified by PCR using a pair of primers derived from the alpha toxin gene. All of 8 suspected isolates were found to be C. perfringens by conventional methods and PCR. Isolates were …
Isolation Of Arcanobacterium (Actinomyces) Pyogenes From Abscessed Cattle Kidney And Identification By Pcr, Hasan Basri̇ Ertaş, Ayşe Kiliç, Gökben Özbey, Adi̇le Muz
Isolation Of Arcanobacterium (Actinomyces) Pyogenes From Abscessed Cattle Kidney And Identification By Pcr, Hasan Basri̇ Ertaş, Ayşe Kiliç, Gökben Özbey, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle. A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 (40%) of the samples examined. DNA extracted from the isolates were amplified by Polymerase Chain Reaction (PCR) using specific primers derived from plo gene of A. pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may …
Cloning And Expression Of Bacteriophage T4 Lysozyme Gene (Gene E) In Escherichia Coli Via Pcr Amplification, Ali̇ İrfan Güzel, Numan Özcan, Hali̇l Kasap
Cloning And Expression Of Bacteriophage T4 Lysozyme Gene (Gene E) In Escherichia Coli Via Pcr Amplification, Ali̇ İrfan Güzel, Numan Özcan, Hali̇l Kasap
Turkish Journal of Veterinary & Animal Sciences
Lysozyme (EC 3.2.1.17) is an enzyme that catalyzes the hydrolysis of \beta(1-->4) glycosidic bonds between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), which are present in peptidoglycan heteropolymers of the prokaryotic cell wall. The purpose of this study was the cloning and expression of the bacteriophage T4 lysozyme gene (gene e) via PCR amplification in Escherichia coli using the pUC18 and pRS416 vectors. PCR amplification is carried out by using the primers having Bam HI recognition sites at their 5' ends. After digesting the vectors and PCR product with Bam HI endonuclease, two recombinant plasmids were constructed by ligation reaction …
Detection And Rflp Analysis Of Canine Parvovirus (Cpv) Dna By Polymerase Chain Reaction (Pcr) In A Dog, Aykut Özkul, İhsan Keleş, Taner Karaoğlu, Mehmet Çabalar, İbrahi̇m Burgu
Detection And Rflp Analysis Of Canine Parvovirus (Cpv) Dna By Polymerase Chain Reaction (Pcr) In A Dog, Aykut Özkul, İhsan Keleş, Taner Karaoğlu, Mehmet Çabalar, İbrahi̇m Burgu
Turkish Journal of Veterinary & Animal Sciences
In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.
Detection Of Cattle Infected With Theileria Annulata In Fields By Nested Pcr, Ifat And Microscopic Examination Of Blood Smears, Zati̇ Vatansever, Serpi̇l Nalbantoğlu
Detection Of Cattle Infected With Theileria Annulata In Fields By Nested Pcr, Ifat And Microscopic Examination Of Blood Smears, Zati̇ Vatansever, Serpi̇l Nalbantoğlu
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to diagnose Theileria annulata infected cattle by microscopy, IFAT and nested PCR and to compare the sensitivity and specificity of these diagnostic methods. A total of 147 blood and sera samples were collected from healthy cattle in four localities of Polatlı district, Ankara, where tropical theileriosis is prevalent. Examination results revealed positivity rates of 31.3%, 44.9% and 61.2% for microscopy, IFAT and nested PCR, respectively. It was shown that nested PCR is more sensitive and specific when compared to microscopical examination and serological findings. It was also shown that nested PCR is more sensitive …
Identification Of Chicken Originated Campylobacter Coli And Campylobacter Jejuni By Polymerase Chain Reaction (Pcr), Hasan Basri̇ Ertaş, Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Öngör
Identification Of Chicken Originated Campylobacter Coli And Campylobacter Jejuni By Polymerase Chain Reaction (Pcr), Hasan Basri̇ Ertaş, Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Öngör
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to isolate Campylobacter species from the intestines and livers of chicken and to identify Campylobacter coli and Campylobacter jejuni by both conventional methods and Polymerase Chain Reaction (PCR). Four specific primers derived from the ceuE gene present in the genomes of C. coli and C. jejuni were used for PCR identification. In the examination of 150 intestine and liver samples by culture and PCR, 25 (16.6%) and 32 (21.3%) were identified as C. coli and C. jejuni, respectively. It was concluded that the PCR assay used in this study may successfully be applied for …
Detection Of Leptospira Species By Polymerase Chain Reaction (Pcr) In Urine Of Cattle, Burhan Çeti̇nkaya, Hasan Basri̇ Ertaş, Hasan Öngör, Adi̇le Muz
Detection Of Leptospira Species By Polymerase Chain Reaction (Pcr) In Urine Of Cattle, Burhan Çeti̇nkaya, Hasan Basri̇ Ertaş, Hasan Öngör, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to investigate the prevalance of leptospirosis in the urine of cattle slaughtered in three major abattoirs in the east of Turkey. A polymerase chain reaction (PCR) based on a pair of genus-specific primers was used to detect leptospiral DNA in the urine samples of 473 cattle, 284 of which were from Elazig, 112 from Malatya and 77 from Diyarbakir. The detection limit of the method was determined to be approximately five bacteria per ml of urine. In the examination of urine samples, 4.02% (19/473) (95% confidence intervals [Cl] 2.4-6.2) were found to be positive by …