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Intracellular Ph Determination By Means Of Ph Glass Microelectrodes, Norman W. Carter

MCV/Q, Medical College of Virginia Quarterly

A number of years ago I decided to attempt to measure the intracellular pH of skeletal muscle by a more direct method than those in current use, that is, by an electrode. I excluded all forms of pH electrodes except the glass electrode and then set up a certain number of characteristics or requirements that this electrode should have. First, the electrode should be insulated in such a manner that only the pH-sensitive portion is in the cell cytoplasm. Second, the reference side of the electrode group should also be within the cell, because the cell has a transmembrane potential; …