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Full-Text Articles in Medicine and Health Sciences
Pushing The Limits: Increasing The Speed And Specificity Of Sars-Cov-2 Testing, Grayson Way
Pushing The Limits: Increasing The Speed And Specificity Of Sars-Cov-2 Testing, Grayson Way
Theses and Dissertations
The prevalence and spread of the current COVID-19 pandemic have highlighted the importance of continual improvements upon current microbiological testing methods. Rapid and accurate testing can help mitigate spread by improving on the time to quarantine and quarantine duration required. As of the writing of this thesis, COVID-19 has been responsible for more than 500,000 deaths in the United States of America, and greater than 2 million deaths globally. The work done in this thesis has shown improvements in the current SARS-CoV-2 testing methodology by reducing the time it takes for patient testing while maintaining accuracy and the sensitivity required …
Approaches To Identify Surface Proteins Of Anaplasma Phagocytophilum Dense-Cored Organisms As Adhesins To Human P-Selectin Glycoprotein Ligand-1, Matthew Troese
Approaches To Identify Surface Proteins Of Anaplasma Phagocytophilum Dense-Cored Organisms As Adhesins To Human P-Selectin Glycoprotein Ligand-1, Matthew Troese
Theses and Dissertations
Anaplasma phagocytophilum is an obligatory intracellular bacterium that infects neutrophils to cause human granulocytic anaplasmosis. Sialyl Lewis x (sLex)-modified P-selectin glycoprotein ligand-1 (PSGL-1) is the confirmed receptor utilized by A. phagocytophilum to bind and invade human neutrophils and myeloid cell lines. As an obligate intracellular pathogen, the binding of A. phagocytophilum to a host cell receptor is a prerequisite step for entry and replication, and thus its survival. However, the bacterial adhesins mediating this process have yet to be identified. In this study, we sought to identify surface proteins of A. phagocytophilum as putative adhesins. A. phagocytophilum undergoes a biphasic …