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Full-Text Articles in Medicine and Health Sciences
On The Spatial Organization Of Mrna, Plasmids, And Ribosomes In A Bacterial Host Overexpressing Membrane Proteins, Lieke A. Van Gijtenbeek, Andrew Robinson, Antoine M. Van Oijen, Bert Poolman, Jan Kok
On The Spatial Organization Of Mrna, Plasmids, And Ribosomes In A Bacterial Host Overexpressing Membrane Proteins, Lieke A. Van Gijtenbeek, Andrew Robinson, Antoine M. Van Oijen, Bert Poolman, Jan Kok
Faculty of Science, Medicine and Health - Papers: part A
By using fluorescence imaging, we provide a time-resolved single-cell view on coupled defects in transcription, translation, and growth during expression of heterologous membrane proteins in Lactococcus lactis. Transcripts encoding poorly produced membrane proteins accumulate in mRNA-dense bodies at the cell poles, whereas transcripts of a well-expressed homologous membrane protein show membrane-proximal localization in a translation-dependent fashion. The presence of the aberrant polar mRNA foci correlates with cessation of cell division, which is restored once these bodies are cleared. In addition, activation of the heat-shock response and a loss of nucleoid-occluded ribosomes are observed. We show that the presence of a …
Organization Of Brainstem Nuclei, George Paxinos, Xu-Feng Huang, Gulgun Sengul, Charles Watson
Organization Of Brainstem Nuclei, George Paxinos, Xu-Feng Huang, Gulgun Sengul, Charles Watson
Faculty of Health and Behavioural Sciences - Papers (Archive)
This chapter describes human homologs of nuclei identified in the brainstem of other mammals and attempts to extend to the human the overall organizational schemata that have been proposed for the brainstem of other mammalian species. We present herein updated diagrams of the Atlas of the Human Brainstem (Paxinos and Huang, 1995). The diagrams have been thoroughly revised in light of our recent work on the rat (Paxinos and Watson, 2007) and rhesus monkey (Paxinos et al., 3rd ed, in BrainNavigator, Elsevier, 2010) as well as our work on the marmoset (Atlas of the Marmoset Brain in Stereotaxic Coordinates, Paxinos …