Medicine and Health Sciences Commons^™

Reducing Dangerous Nighttime Events In Persons With Dementia Using A Nighttime Monitoring System, Meredeth A. Rowe, Annette Kelly, Claydell Horne, Steve Lane, Judy Campbell, Brandy Lehman, Chad Phipps, Meredith Keller, Andrea Pe Benito

Nursing Faculty Publications

Background: Nighttime activity, a common occurrence in persons with dementia, increases the risk for injury and unattended home exits, and impairs the sleep patterns of caregivers. Technology is needed that will alert caregivers of nighttime activity in persons with dementia to help prevent injuries and unattended exits. Methods: As part of a product development grant, a randomized pilot study was conducted to test the effectiveness of a new night monitoring system designed for informal caregivers to use in the home. Data from 53 subjects were collected at 9 points in time over a 12-month period regarding injuries and unattended home …

Asset-Based Approaches For Lesbian, Gay, Bisexual, Transgender, Questioning, Intersex, And Two-Spirit (Lgbtqi2-S) Youth And Families In Systems Of Care, Peter E. Gamache, Katherine J. Lazear, Research And Training Center For Children's Mental Health

FMHI Publications

No abstract provided.

Self-Assessment/Planning Tool For Implementing Recovery-Oriented Mental Health Services (Sapt), James Winarski, Michael G. Dow, Patrick Hendry, Patricia Robison, Roger H. Peters

Mental Health Law & Policy Faculty Publications

No abstract provided.

Utilization Of The Most Costly And Intense Mental Health Services Among Children In Out-Of-Home Care, Svetlana Yampolskaya, Paul E. Greenbaum, Colleen Clark, Richard Briscoe

Mental Health Law & Policy Faculty Publications

No abstract provided.

Immunomodulatory Effects Of Novel Therapies For Stroke, Aaron A. Hall

USF Tampa Graduate Theses and Dissertations

Each year, approximately 795,000 people suffer a new or recurrent stroke. About 610,000 of these are first attacks, and 185,000 are recurrent attacks (Carandang et al. 2006). Currently the only FDA approved treatment for ischemic stroke is recombinant tissue plasminogen activator (Alteplase) (Marler and Goldstein 2003). Unfortunately its use is restricted to a short, 4.5 hour, time window. Two promising therapies in the treatment of stroke at delayed timepoints are human umbilical cord blood cells (HUCBC) and the sigma receptor agonist DTG

The first series of experiments were conducted to characterize the effects of sigma receptors on various aspects of …

Emergency Commitment Of Very Young Children, Annette Christy, Brittany Handelsman

Mental Health Law & Policy Faculty Publications

No abstract provided.

Success Strategies For Thesis Students: Creating A Video Toolbox, Claudia J. Dold

Claudia J. Dold

With an increase in our pool of users and a cap on hiring, the librarians in our shop needed a way to clone themselves in order to deliver the same good service on which we pride ourselves and on which our constituency has come to rely. Our proposed solution was to create video segments on how to use the library effectively. Funded by a grant, a project was undertaken to produce a series of fifteen videos designed to guide students through the research and writing skills that their program chairs deem essential for top-quality work. This paper presents the thought …

Quantitative Literacy On The Web Of Science, 2 – Mining The Health Numeracy Literature For Assessment Items, H. Len Vacher, Todd A. Chavez

Todd A. Chavez

A topic search of the Web of Science (WoS) database using the term “numeracy” produced a bibliography of 293 articles, reviews and editorial commentaries (Oct 2008). The citation graph of the bibliography clearly identifies five benchmark papers (1995-2001), four of which developed numeracy assessment instruments. Starting with the 80 papers that cite these benchmarks, we identified a set of 25 papers (1995-2008) in which the medical research community reports the development and/or application of health-numeracy assessments. In all we found 10 assessment instruments from which we have compiled a total of 48 assessment items. There are both general and context-specific …

Quantitative Literacy On The Web Of Science, 2 – Mining The Health Numeracy Literature For Assessment Items, H. Len Vacher, Todd A. Chavez

Academic Resources Faculty and Staff Publications

A topic search of the Web of Science (WoS) database using the term “numeracy” produced a bibliography of 293 articles, reviews and editorial commentaries (Oct 2008). The citation graph of the bibliography clearly identifies five benchmark papers (1995-2001), four of which developed numeracy assessment instruments. Starting with the 80 papers that cite these benchmarks, we identified a set of 25 papers (1995-2008) in which the medical research community reports the development and/or application of health-numeracy assessments. In all we found 10 assessment instruments from which we have compiled a total of 48 assessment items. There are both general and context-specific …

Characterization Of Nprc And Its Binding Partners, Abdel A. Alli

USF Tampa Graduate Theses and Dissertations

The C type natriuretic peptide receptor (NPRC) also known as NPR3 is a widely expressed single transmembrane-spanning protein. NPRC functions as a homodimer at the cell surface for the metabolic clearance of a broad range of natriuretic peptides from circulation. The intracellular domain of NPRC is coupled to inhibitory G proteins and is involved in mediating signal transduction. In order to further elucidate the role of NPRC in signal transduction a proteomic approach was taken to identify putative protein binding partners for NPRC in different cell-types. An interrogation of the molecular association between NPRC and its identified protein binding partner(s) …

Protein Disorder In The Human Diseasome: Unfoldomics Of Human Genetic Diseases, Uros Midic, Christopher J. Oldfield, A. Keith Dunker, Zoran Obradovic, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Background: Intrinsically disordered proteins lack stable structure under physiological conditions, yet carry out many crucial biological functions, especially functions associated with regulation, recognition, signaling and control. Recently, human genetic diseases and related genes were organized into a bipartite graph (Goh KI, Cusick ME, Valle D, Childs B, Vidal M, et al. (2007) The human disease network. Proc Natl Acad Sci U S A 104: 8685–8690). This diseasome network revealed several significant features such as the common genetic origin of many diseases.

Methods and findings: We analyzed the abundance of intrinsic disorder in these diseasome network proteins by means of several …

Substance P Increases Cell-Surface Expression Of Cd74 (Receptor For Macrophage Migration Inhibitory Factor): In Vivo Biotinylation Of Urothelial Cell-Surface Proteins, Katherine L. Meyer-Siegler, Shen-Ling Xia, Pedro L. Vera

Molecular Medicine Faculty Publications

Macrophage migration inhibitory factor (MIF), an inflammatory cytokine, and its receptor CD74 are upregulated by bladder inflammation. MIF-mediated signal transduction involves binding to cell-surface CD74, this study documents, in vivo, MIF-CD74 interactions at the urothelial cell surface. N-hydroxysulfosuccinimide biotin ester-labeled surface urothelial proteins in rats treated either with saline or substance P (SP, 40 μg/kg). The bladder was examined by histology and confocal microscopy. Biotinylated proteins were purified by avidin agarose, immunoprecipitated with anti-MIF or anti-CD74 antibodies, and detected with strepavidin-HRP. Only superficial urothelial cells were biotinylated. These cells contained a biotinylated MIF/CD74 cell-surface complex that was increased in …

The Mysterious Unfoldome: Structureless, Underappreciated, Yet Vital Part Of Any Given Proteome, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Contrarily to the general believe, many biologically active proteins lack stable tertiary and/or secondary structure under physiological conditions in vitro. These intrinsically disordered proteins (IDPs) are highly abundant in nature and many of them are associated with various human diseases. The functional repertoire of IDPs complements the functions of ordered proteins. Since IDPs constitute a significant portion of any given proteome, they can be combined in an unfoldome; which is a portion of the proteome including all IDPs (also known as natively unfolded proteins, therefore, unfoldome), and describing their functions, structures, interactions, evolution, and so forth. Amino acid sequence and …

Expression Of Semaphorin 3f And Its Receptors In Epithelial Ovarian Cancer, Fallopian Tubes, And Secondary Müllerian Tissues, Christina D. Drenberg, Sandra Livingston, Ren Chen, Patricia A. Kruk, Santo V. Nicosia

Pathology and Cell Biology Faculty Publications

While semaphorins and their receptors appear to play a role in tumor carcinogenesis, little is known about the role of semaphorin 3F (S3F) in epithelial ovarian cancer (EOC) development. Therefore, we sought to determine the clinical relationship between S3F and its receptors, neuropilin-2 (NP-2) and neuropilin-1 (NP-1) with EOC progression. We analyzed the immunohistological expression of S3F, NP-2, and NP-1 in clinical specimens of normal ovaries (N), benign cystadenomas (Cy), well-differentiated adenocarcinomas (WD), poorly-differentiated adenocarcinomas (PD), inclusion cysts (IC), paraovarian cysts (PC), and fallopian tubes (FT). Tissue sections were evaluated for staining intensity and percentage of immunoreactive epithelia. We found …

A Comparative Analysis Of Structural Brain Mri In The Diagnosis Of Alzheimer’S Disease, Jason Appel, Elizabeth Potter, Qian Shen, Gustavo Pantol, Maria T. Greig, David Loewenstein, Ranjan Duara

Neurology Faculty Publications

Dementia is a debilitating and life-altering disease which leads to both memory impairment and decline of normal executive functioning. While causes of dementia are numerous and varied, the leading cause among patients 60 years and older is Alzheimer’s disease. The gold standard for Alzheimer’s diagnosis remains histological identification of amyloid plaques and neurofibrillary tangles within the medial temporal lobe, more specifically the entorhinal cortex and hippocampus. Although no definitive cure for Alzheimer's disease currently exists, there are treatments targeted at preserving cognition and memory while delaying continued loss of function. Alzheimer's disease exists along a spectrum of cognitive decline and …

Influence Of Sequence Changes And Environment On Intrinsically Disordered Proteins, Amrita Mohan, Vladimir N. Uversky, Predrag Radivojac

Molecular Medicine Faculty Publications

Many large-scale studies on intrinsically disordered proteins are implicitly based on the structural models deposited in the Protein Data Bank. Yet, the static nature of deposited models supplies little insight into variation of protein structure and function under diverse cellular and environmental conditions. While the computational predictability of disordered regions provides practical evidence that disorder is an intrinsic property of proteins, the robustness of disordered regions to changes in sequence or environmental conditions has not been systematically studied. We analyzed intrinsically disordered regions in the same or similar proteins crystallized independently and studied their sensitivity to changes in protein sequence …

Unfoldomics Of Human Diseases: Linking Protein Intrinsic Disorder With Diseases, Vladimir N. Uversky, Christopher J. Oldfield, Uros Midic, Hongbo Xie, Bin Xue, Slobodan Vucetic, Lilia M. Iakoucheva, Zoran Obradovic, A. Keith Dunker

Molecular Medicine Faculty Publications

Background: Intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs) lack stable tertiary and/or secondary structure yet fulfills key biological functions. The recent recognition of IDPs and IDRs is leading to an entire field aimed at their systematic structural characterization and at determination of their mechanisms of action. Bioinformatics studies showed that IDPs and IDRs are highly abundant in different proteomes and carry out mostly regulatory functions related to molecular recognition and signal transduction. These activities complement the functions of structured proteins. IDPs and IDRs were shown to participate in both one-to-many and many-to-one signaling. Alternative splicing and posttranslational modifications …

Protein Intrinsic Disorder And Influenza Virulence: The 1918 H1n1 And H5n1 Viruses, Gerard Kian-Meng Goh, A. Keith Dunker, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Background: The 1918 H1N1 virus was a highly virulent strain that killed 20–50 million people. The cause of its virulence remains poorly understood.

Methods: Intrinsic disorder predictor PONDR® VLXT was used to compare various influenza subtypes and strains. Three-dimensional models using data from X-ray crystallographic studies annotated with disorder prediction were used to characterize the proteins.

Results: The protein of interest is hemagglutin (HA), which is a surface glycoprotein that plays a vital role in viral entry. Distinct differences between HA proteins of the virulent and non-virulent strains are seen, especially in the region near residues 68–79 of the HA2. …

Are Morphological Specializations Of The Hyolingual System In Chameleons And Salamanders Tuned To Demands On Performance?, Anthony Herrel, Stephen M. Deban, Vicky Schaerlaeken, Jean-Pierre Timmermans, D. Adriaens

Integrative Biology Faculty and Staff Publications

Extremely specialized and long tongues used for prey capture have evolved independently in plethodontid salamanders and chameleons. In both systems, the demands on tongue projection are probably similar: to maximize projection velocity and distance. Consequently, many of the design features of the projection system in these two groups have converged to an astonishing degree. Both involve the use of power amplification systems based on helically wound muscle fibers that load internal connective tissue sheets as illustrated in previous studies. Demands imposed on tongue retraction, however, are different to some degree. Although in both groups there is a clear demand for …

System Of Care Implementation, Robert M. Friedman, Paul E. Greenbaum, Wei Wang, Krista Kutash, Roger A. Boothroyd

Mental Health Law & Policy Faculty Publications

For almost 25 years now, a major policy emphasis in children’s mental health at the federal level and within most states has been the development of community-based systems of care. There have been many meetings and conferences about this, training and technical assistance material has been provided, and written material has been prepared, and a federal grant program, the Children’s Mental Health Initiative (CMHI) has now provided funding to over 100 grantees for the development of such systems of care.

Evaluation Of An Intervention For Adolescent Girls With Trauma Related Disorders, Meredith Elzy, Sarah Cobb, Norin Dollard, Teresea Van Alstine, Colleen Clark

Mental Health Law & Policy Faculty Publications

No abstract provided.