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Full-Text Articles in Medicine and Health Sciences
Regulation Of C-Type Natriuretic Peptide Expression, Donald F. Sellitti, Nancy Koles, Maria C. Mendonça
Regulation Of C-Type Natriuretic Peptide Expression, Donald F. Sellitti, Nancy Koles, Maria C. Mendonça
Uniformed Services University of the Health Sciences
C-type natriuretic peptide (CNP) is a member of the small family of natriuretic peptides that also includes atrial natriuretic peptide (ANP) and brain, or B-type natriuretic peptide (BNP). Unlike them, it performs its major functions in an autocrine or paracrine manner. Those functions, mediated through binding to the membrane guanylyl cyclase natriuretic peptide receptor B (NPR-B), or by signaling through the non-enzyme natriuretic peptide receptor C (NPR-C), include the regulation of endochondral ossification, reproduction, nervous system development, and the maintenance of cardiovascular health. To date, the regulation of CNP gene expression has not received the attention that has been paid …
Nicotine Induced Cpg Methylation Of Pax6 Binding Motif In Star Promoter Reduces The Gene Expression And Cortisol Production, Tingting Wang, Man Chen, Lian Liu, Huaiyan Cheng, You-E Yan, Ying-Hong Feng, Hui Wang
Nicotine Induced Cpg Methylation Of Pax6 Binding Motif In Star Promoter Reduces The Gene Expression And Cortisol Production, Tingting Wang, Man Chen, Lian Liu, Huaiyan Cheng, You-E Yan, Ying-Hong Feng, Hui Wang
Uniformed Services University of the Health Sciences
Steroidogenic acute regulatory protein (StAR) mediates the rate-limiting step in the synthesis of steroid hormones, essential to fetal development. We have reported that the StAR expression in fetal adrenal is inhibited in a rat model of nicotine-induced intrauterine growth retardation (IUGR). Here using primary human fetal adrenal cortex (pHFAC) cells and a human fetal adrenal cell line NCI-H295A, we show that nicotine inhibits StAR expression and cortisol production in a dose- and time-dependent manner, and prolongs the inhibitory effect on cells proliferating over 5 passages after termination of nicotine treatment. Methylation detection within the StAR promoter region uncovers a single …
Identification Of Mycobacterium Paratuberculosis Gene Expression Signals, John P. Bannantine, Raul G. Barletta, Charles O. Thoen, Robert E. Andrews Jr.
Identification Of Mycobacterium Paratuberculosis Gene Expression Signals, John P. Bannantine, Raul G. Barletta, Charles O. Thoen, Robert E. Andrews Jr.
School of Veterinary and Biomedical Sciences: Faculty Publications
Mycobacterium paratuberculosis promoter-containing clones were isolated from a genomic DNA library constructed in the transcriptional-translational fusion vector pYUB76. The promoter-containing DNA fragments were identified in the surrogate host Mycobacterium smegmatis by expression of the promoterless lacZ reporter gene of pYUB76. The expression signals exhibited a wide range of strengths, as indicated by their corresponding β-galactosidase activities. Eight clones were sequenced and characterized further. Predicted open reading frames and codon usage were identified by computer analysis. Database searching for related sequences using the BLAST method revealed no homologies. Transcriptional activity was measured by slot-blot hybridization with steady-state RNA isolated from lacZ …