Medicine and Health Sciences Commons^™

Neuromagnetic Measures Of Word Processing In Bilinguals And Monolinguals, Yingying Wang, Jing Xiang, Jennifer Vannest, Tom Holroyd, Daria Narmoneva, Paul Horn, Yinhong Liu, Douglas Rose, Ton Degrauw, Scott Holland

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

Objective: This study aimed to use magnetoencephalography (MEG) to examine the question of whether Mandarin-English bilingual speakers recruit the same cortical areas or develop distinct language-specific networks without overlaps for word processing.

Methods: Eight healthy Mandarin-English bilingual adults and eight healthy English monolingual adults were scanned while single-word paradigms were audio-visually presented.

Results: Our results showed significantly stronger beta-band power suppression in the right inferior parietal lobe (IPL) covering the supramarginal gyrus (BA 40) and angular gyrus (BA 39) for bilinguals when processing Mandarin versus English. Moreover, there were no significant differences between bilinguals and monolinguals in the left inferior …

Advances And Future Challenges In Adenoviral Vector Pharmacology And Targeting, Reeti Khare, Christopher Y. Chen, Eric A. Weaver, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Adenovirus is a robust vector for therapeutic applications, but its use is limited by our understanding of its complex in vivo pharmacology. In this review we describe the necessity of identifying its natural, widespread, and multifaceted interactions with the host since this information will be crucial for efficiently redirecting virus into target cells. In the rational design of vectors, the notion of overcoming a sequence of viral “sinks” must be combined with re-targeting to target populations with capsid as well as shielding the vectors from pre-existing or toxic immune responses. It must also be noted that most known adenoviral pharmacology …

Assessment Of Pathways For The Introduction And Spread Of Mycobacterium Bovis In The United States, Katie Portacci, Jason Lombard, Lauren Abrahamsen, Eric Bush, Charles Fossler, Robert Harris, Kamina Johnson, Ryan S. Miller, Dianna Mitchell, Randy Pritchard, Steven Sweeney, Todd Weaver

Other Publications in Zoonotics and Wildlife Disease

Bovine tuberculosis (TB) was responsible for more losses among U.S. farm animals in the early 20th century than all other infectious diseases combined. The Cooperative State-Federal Tuberculosis Eradication Program (established in 1917 and administered by APHIS, State animal health agencies, and U.S. livestock producers) has nearly eradicated bovine TB from the nation’s livestock population. However, despite the many accomplishments of the program, bovine TB remains a serious and costly disease of livestock in the United States. In 1992, VS conducted an assessment to identify pathways for the introduction and spread of bovine TB, in order to develop the most effective …

Low Levels Of Hydrogen Peroxide Stimulate Corneal Epithelial Cell Adhesion, Migration, And Wound Healing, Qing Pan, Wen-Ya Qiu, Ya-Nan Huo, Yu-Feng Yao, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

PURPOSE. Intracellular reactive oxygen species have been reported to associate with growth factor and integrin signalings in promoting cell adhesion in many cell types. This study is to explore if exogenous H2O2 at low levels can be beneficial to cell adhesion, migration, and wound healing.

METHODS. Primary rabbit corneal epithelial cells treated with 0–70 M H2O2 were tested for viability by MTT assay, adhesion by centrifugation assay, focal contacts of vinculin and F-actin by immunofluorescence, activated Src(pY416), EGF receptor (pY845), vinculin(pY1065), FAK(pY397), and FAK(pY576) by immunoblotting. Cell migration was examined with 0–50 M H2O2 using the scratch wound technique. Corneal …

The Traumatic Stress Response In Child Maltreatment And Resultant Neuropsychological Effects, Kathryn R. Wilson, David J. Hansen, Ming Li

Department of Psychology: Faculty Publications

Child maltreatment is a pervasive problem in our society that has long-term detrimental consequences to the development of the affected child such as future brain growth and functioning. In this paper, we surveyed empirical evidence on the neuropsychological effects of child maltreatment, with a special emphasis on emotional, behavioral, and cognitive process–response difficulties experienced by maltreated children. The alteration of the biochemical stress response system in the brain that changes an individual’s ability to respond efficiently and efficaciously to future stressors is conceptualized as the traumatic stress response. Vulnerable brain regions include the hypothalamic–pituitary–adrenal axis, the amygdala, the hippocampus, and …

Olanzapine And Risperidone Disrupt Conditioned Avoidance Responding By Selectively Weakening Motivational Salience Of Conditioned Stimulus: Further Evidence, Chen Zhang, Yiru Fang, Ming Li

Department of Psychology: Faculty Publications

Suppression of conditioned avoidance response is a preclinical behavioral index of antipsychotic activity. Previous work shows that olanzapine and risperidone disrupt avoidance response elicited by a less salient conditioned stimulus (CS2) to a greater extent than avoidance elicited by a more salient stimulus (CS1), suggesting that antipsychotic drugs may have a weakening action on motivational salience of stimuli. In the present study, we further examined this mechanism of antipsychotic action, focusing on the possible impact of baseline difference of CS1 and CS2 response rates on the avoidance-disruptive effect of olanzapine and risperidone. Rats were first trained to acquire avoidance responding …

The Evolution Of Prophy Paste, Caren M. Barnes

College of Dentistry: Faculty Publications

Dental hygienists have many products to choose from when deciding which prophy paste is best for each patient. To date, the evidence supporting prophy pastes formulated to remineralize enamel or eliminate dentinal hypersensitivity is somewhat unclear. Most of the research has been conducted in vitro among environments that do not accurately simulate clinical conditions. Additional clinical research performed in vivo is needed to provide the evidence base necessary for effective clinical decision making.

Factors Affecting Space Use Overlap By White-Tailed Deer In An Urban Landscape, W. David Walter, Jeff Beringer, Lonnie P. Hansen, Justin W. Fischer, Joshua J. Millspaugh, Kurt C. Vercauteren

USDA Wildlife Services: Staff Publications

Variation in the size and overlap of space use by white-tailed deer (Odocoileus virginianus) has broad implications for managing deer–human conflicts and disease spread and transmission in urban landscapes. Understanding which factors affect overlap of home range by various segments (i.e., age, sex) of an urban deer population has implications to direct contact between deer on disease epidemiology. We assessed size of home range and overlap of space use using the volume of intersection index (VI) for deer in an urban landscape by sex, age, season, and time of day. We found mean space use was larger for …

Sleep Assessments In Healthy School-Aged Children Using Actigraphy: Concordance With Polysomnography, Karen Spruyt, David Gozal, Ehab Dayyat, Adrienne Roman, Dennis L. Molfese

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

Actigraphic recordings (ACT) are widely used in school children as a less intrusive and more extended approach to evaluation of sleep problems. However, critical assessment of the validity and reliability of ACT against overnight polysomnography (NPSG) are unavailable. Thus, we explored the degree of concordance between NPSG and ACT in school-aged children to delineate potential ACT boundaries when interpreting pediatric sleep. Non-dominant wrist ACT was simultaneously recorded with NPSG in 149 healthy school-aged children (4.1 to 8.8 years old, 41.7% boys and 80.4% Caucasian) recruited from the community. Analyses were limited to the Actiware (MiniMitter-64) calculated parameters originating from 1-min …

Policy And Practice: An Analysis Of The Implementation Of Supported Employment In Nebraska, Heng-Hsian N. Liu

Department of Psychology: Dissertations, Theses, and Student Research

Supported employment (SE) is an evidence-based practice (EBP) for persons with severe mental illness (SMI) aimed at competitive employment. SE has a large evidence base, demonstrating outcomes across settings and populations. SE has been promoted by the Centers for Medicare and Medicaid Services (in the U.S. Department of Health and Human Services) and widely disseminated through the internet via a “community tool-kit” sponsored by the Substance Abuse and Mental Health Services Administration.

The SE literature expresses the opinion that state governments can successfully implement SE. Researchers have developed implementation guidelines and identified stages of statewide implementation; however, most SE implementation …

Organs Watch: Possibilities And Perils For Public Anthropology, Roberto Abadie

Department of Sociology: Faculty Publications

Recent technological advances in biomedicine have introduced new therapeutic possibilities but have also contributed to the emergence of a global market for human bodies and body parts. For example, artificial modes of human reproduction created a market for eggs, semen, and surrogate wombs. In addition, organ transfer generated a demand for kidneys and half livers. The whole body has become a valuable commodity as professional research subjects venture into the economy of Phase I Clinical Trials, testing drug safety for pharmaceutical companies. In the process, the trade has become a deeply unequal one in which poor, vulnerable, and easily exploited …

Juror Perceptions Of Juveniles Transferred To Criminal Court: The Role Of Generic Prejudice And Emotion In Determinations Of Guilt, Megan Beringer Jones

Department of Psychology: Dissertations, Theses, and Student Research

Research examining juror perceptions of juveniles tried as adults has provided mixed results, with some studies providing evidence of bias against juveniles tried as adults, and others finding no evidence of this bias. The present research aimed to clarify this issue by examining the roles of generic prejudice and emotion in jurors’ judgments of juveniles tried as adults. Study 1 assessed which stereotypes people associate with juveniles tried as adults compared to juveniles tried in juvenile court and adults tried in criminal court. Study 2 examined to what extent angry, fearful, sad, and neutral mock jurors used these stereotypes to …

Ready, Set...Pitch: Marketing Yourself Like An Entrepreneur, Connie I. Reimers-Hild

Kimmel Education and Research Center: Presentations and White Papers

Great elevator pitches are created to sell your idea, business, product or service in 30 seconds or less (the time it takes to go from the first floor to the top of the building in an elevator). It’s important to develop and practice an effective pitch so you are ready to use it under fire. When you step onto an elevator with Warren Buffett or Bill Gates, you want to be prepared! This worksheet will help you create an amazing pitch!!

Behavioral Health Providers And Electronic Health Records: An Exploratory Beliefs Elicitation And Segmentation Study, Nancy C. Shank

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The widespread adoption of electronic health records (EHRs) is a public policy strategy to improve healthcare quality and reduce accelerating health care costs. Much research has focused on medical providers’ perceptions of EHRs, but little is known about those of behavioral health providers. This research was informed by the theory of reasoned action, and the technology acceptance model. This mixed methods research was conducted in two studies. The first study interviewed behavioral health providers (n = 32) to elicit beliefs about EHRs. Using the elicited beliefs from the first study, a survey of 38 Likert-scaled belief statements was administered to …

Prevalence And Natural History Of Neuropsychiatric Syndromes In Veteran Hospice Patients, Elizabeth R. Goy, Linda Ganzini

Public Health Resources

Context. Prospective studies are needed to adequately describe the overall impact of neuropsychiatric syndromes on the course of hospice enrollment in outpatient settings.

Objectives. To determine the prevalence and natural history of delirium, cognitive impairment, alcohol abuse, anxiety, depression, and suicidal ideation (SI) in community-dwelling veteran hospice patients.

Methods. Home hospice patients were visited regularly from enrollment until their deaths, study withdrawal, or discharge from hospice. Family caregivers gave consent for those with Mini-Mental State Examination (MMSE) scores less than or equal to 23. Measures included the Structured Clinical Interview for DSM-IV for depression (past and current) …

Characterization Of Igg And Ige Binding To Parvalbumin Derived From Commercially Important Fish Species, P. Lee, J. A. Nordlee, S. J. Koppelman, J. L. Baumert, S. L. Taylor

Department of Food Science and Technology: Faculty Publications

Rationale: Parvalbumin is recognized as pan-allergen in fish and frog. However, previous studies demonstrated that the IgE- and IgG-binding patterns to parvalbumins vary depending on the fish species. We aimed to use 3 anti-parvalbumin IgG and human IgE to investigate the contributing factors for the binding differences.

Methods: Indirect enzyme-linked immunosorbent assay (ELISA) and IgG immunoblotting were used to determine the reactivity of the polyclonal anti-cod parvalbumin antibody and the commercially available, monoclonal anti-frog and anti-carp parvalbumin antibodies against raw muscle extracts of 25 fish species. Additionally, sera from 46 individuals with clinical history of fish allergy were analyzed for …

Sleep Duration, Sleep Regularity, Body Weight, And Metabolic Homeostasis In School-Aged Children, Karen Spruyt, Dennis L. Molfese, David Gozal

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

OBJECTIVE: The goal was to explore the effects of duration and regularity of sleep schedules on BMI and the impact on metabolic regulation in children.

METHODS: Sleep patterns of 308 community-recruited children 4 to 10 years of age were assessed with wrist actigraphs for 1 week in a cross-sectional study, along with BMI assessment. Fasting morning plasma levels of glucose, insulin, lipids, and high-sensitivity C-reactive protein also were measured for a subsample.

RESULTS: Children slept 8 hours per night, on average, regardless of their weight categorization. A nonlinear trend between sleep and weight emerged. For obese children, …

Sensory Evaluation Of Ice Cream Made With Prebiotic Ingredients Substituted For Sugar, Jennifer M. Wood

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Prebiotics are non-digestible food ingredients that beneficially affect the host by selectively stimulating growth and/or activity of beneficial bacteria in the colon. The project objective was to determine consumer acceptability of ice cream with prebiotic ingredients substituted for part of the sugar and to determine sensory attributes of sweetness, smoothness, and vanilla flavor. A commercial ice cream mix was made substituting 0%, 10%, 20%, or 30% of the sugar for either Fructooligosaccharides (FOS) or inulin. Sensory analyses were conducted using 95 non-trained panelists. Overall consumer acceptability and sensory attributes were measured on a 175 mm anchored hedonic scale. When 10% …

Ready, Set...Pitch: Marketing Yourself Like An Entrepreneur (Powerpoint Pdf), Connie I. Reimers-Hild

Kimmel Education and Research Center: Presentations and White Papers

Great elevator pitches are created to sell your idea, business, product or service in 30 seconds or less (the time it takes to go from the first floor to the top of the building in an elevator). It’s important to develop and practice an effective pitch so you are ready to use it under fire. When you step onto an elevator with Warren Buffett or Bill Gates, you want to be prepared! This worksheet will help you create an amazing pitch!!

Fluorine Substitutions In An Antigenic Peptide Selectively Modulate T Cell Receptor Binding In A Minimally Perturbing Manner, Kurt H. Piepenbrink, Oleg Y. Borbulevych, Ruth F. Sommese, John Clemens, Kathrynq M. Armstrong, Clare Desmond, Priscilla Do, Brian M. Baker

Food for Health: Publications

T cell receptor (TCR) recognition of antigenic peptides bound and presented by major histocompatibility complex (MHC) molecules forms the basis of the cellular immune response to pathogens and cancer. TCRs bind peptide/MHC molecules weakly and with fast kinetics, features which have hindered detailed biophysical studies of these interactions. Modified peptides resulting in enhanced TCR binding could help overcome these challenges. Further, there is considerable interest in using modified peptides with enhanced TCR binding as the basis for clinical vaccines. Here, we studied how fluorine substitutions in an antigenic peptide can selectively impact TCR recognition. Using a structure-guided design approach, we …

Mir-27b*, An Oxidative Stress-Responsive Microrna Modulates Nuclear Factor-Kb Pathway In Raw 264.7 Cells, Sivasubramani Thulasingam, Chandirasegaran Massilamany, Arunakumar Gangaplara, Hongjiu Dai, Shahlo Yarbaeva, Sakthivel Subramaniam, Jean-Jack Riethoven, James Eudy, Marjorie F. Lou, Jay Reddy

Jay Reddy Publications

Reactive oxygen species (ROS) produced in macrophages is critical for microbial killing, but they also take part in inflammation and antigen presentation functions. MicroRNAs (miRNAs) are endogenous regulators of gene expression, and they can control immune responses. To dissect the complex nature of ROS-mediated effects in macrophages, we sought to characterize miRNAs that are responsive to oxidative stress-induced with hydrogen peroxide (H₂O₂) in the mouse macrophage cell line, RAW 264.7. We have identified a set of unique miRNAs that are differentially expressed in response to H₂O₂. These include miR-27a*, miR-27b*, miR-29b*, miR-24-2*, …

Identification Of A Second Mimicry Epitope From Acanthamoeba Castellanii That Induces Cns Autoimmunity By Generating Cross-Reactive T Cells For Mbp 89–101 In Sjl Mice, Chandirasegaran Massilamany, Oluwatoyin A. Asojo, Arunakumar Gangaplara, David J. Steffen, Jay Reddy

Jay Reddy Publications

We had previously reported that Acanthamoeba castellanii (ACA) contains a mimicry epitope for proteolipid protein 139–151 capable of inducing central nervous system (CNS) autoimmunity in SJL/J mice. We now present evidence that ACA also contains a mimicry epitope for myelin basic protein (MBP) 89–101, a derivative from amoebic nicotinamide adenine dinucleotide dehydrogenase subunit 2 (NAD). The epitope, NAD 108–120, contains a discontinuous stretch of six amino acids in the core region (VVFFKNIILIGFL) sharing 46% identity with MBP 89–101 (VHFFKNIVTPRTP; identical residues are underlined). SJL mice immunized with NAD 108–120 develop encephalomyelitis similar to the disease induced by the cognate peptide. …

Giant Viruses, James L. Van Etten

James Van Etten Publications

The common view of viruses, mostly true, is of tiny burglars that sneak into cells, grab the biosynthetic controls and compel the cell to make huge numbers of progeny that break out of the cell and keep the replication cycle going. Viruses are supposed to be diminutive even compared to cells that are just a micrometer (1,000 nanometers) in diameter. They are supposed to travel light, making do with just a few well-adapted genes.

In 1992, a new microorganism was isolated from a power-plant cooling tower in Bradford, England, where Timothy Robotham, a microbiologist at Leeds Public Health Laboratory, was …

Minimal Art: Or Why Small Viral K+ Channels Are Good Tools For Understanding Basic Structure And Function Relations, Gerhard Thiel, Dirk Baumeister, Indra Schroeder, Stefan M. Kast, James L. Van Etten, Anna Moroni

James Van Etten Publications

Some algal viruses contain genes that encode proteins with the hallmarks of K+ channels. One feature of these proteins is that they are less than 100 amino acids in size, which make them truly minimal for a K+ channel protein. That is, they consist of only the pore module present in more complex K+ channels. The combination of miniature size and the functional robustness of the viral K+ channels make them ideal model systems for studying how K+ channels work. Here we summarize recent structure/function correlates from these channels, which provide insight into functional properties such as gating, pharmacology and …

Functional Hak/Kup/Kt-Like Potassium Transporter Encoded By Chlorella Viruses, Timo Greiner, José Ramos, Maria C. Alvarez, James Gurnon, Ming Kang, James L. Van Etten, Anna Moroni, Gerhard Thiel

James Van Etten Publications

Chlorella viruses are a source of interesting membrane transport proteins. Here we examine a putative K+ transporter encoded by virus FR483 and related chlorella viruses. The protein shares sequence and structural features with HAK/KUP/KT-like K+ transporters from plants, bacteria and fungi. Yeast complementation assays and Rb+ uptake experiments show that the viral protein, termed HAKCV (high-affinity K+ transporter of chlorella virus), is functional, with transport characteristics that are similar to those of known K+ transporters. Expression studies revealed that the protein is expressed as an early gene during viral replication, and proteomics data indicate that it is not packaged in …

The United States Army Ocular Teleconsultation Program 2004 Through 2009, Michael J. Mines, Kraig S. Bower, Charles M. Lappan, Robert A. Mazzoli, Ronald K. Poropatich

Uniformed Services University of the Health Sciences

PURPOSE: To describe the United States Army Ocular Teleconsultation program and all consultations received from its inception in July 2004 through December 2009.

DESIGN: Retrospective, noncomparative, consecutive case series.

METHODS: All 301 consecutive ocular teleconsultations received were reviewed. The main outcome measures were differential diagnosis, evacuation recommendations, and origination of consultation. Secondary measures included patient demographics, reason for consultation, and inclusion of clinical images.

RESULTS: The average response time was 5 hours and 41 minutes. Most consultations originated from Iraq (58.8%) and Afghanistan (18.6%). Patient care-related requests accounted for 94.7% of consultations; nonphysicians submitted 26.3% of consultations. Most patients (220/285; …

Educating Clerkship Students In The Era Of Resident Duty Hour Restrictions, L. James Nixon, Meenakshy Aiyer, Steven Durning, Chris Gouveia, Jennifer R. Kogan, Valerie J. Lang, Olle Ten Cate, Karen E. Hauer

Uniformed Services University of the Health Sciences

In 2003, the Accreditation Council for Graduate Medical Education defined for the first time an upper limit on the number of hours that residents can work. This initial reduction in work hours was made with the goal of improving patient safety, resident education, and resident well-being. Now, 8 years later, further duty hour restrictions are slated to take effect July 1, 2011. These new reductions are largely a response to growing public concern about the effects of sleep deprivation on residents. Educators and residents alike have mixed opinions about the effects of prior and future duty hour restrictions on resident …

Anti-Fcεr1 Antibody Injections Activate Basophils And Mast Cells And Delay Type 1 Diabetes Onset In Nod Mice, Marc P. Hübner, David Larson, Marina N. Torrero, Ellen Mueller, Yinghui Shi, Kristin E. Killoran, Edward Mitre

Uniformed Services University of the Health Sciences

Mounting evidence suggests that helminth infections protect against autoimmune diseases. As helminths cause chronic IgE-mediated activation of basophils and mast cells we hypothesized that continuous activation of these cells could prevent diabetes onset in nonobese diabetic (NOD) mice in the absence of infection. Anti-FcεR1 activated basophils and mast cells and resulted in the release of IL-4 and histamine into the bloodstream. Anti-FcεR1-treated NOD mice showed a type 2 shift in insulin-specific antibody production and exhibited significant delays in diabetes onset. IL-4 responses played a partial role as the protective effect of anti-FcεR1 therapy was diminished in IL-4-deficient NOD mice. In …

Arachidonyl Trifluoromethyl Ketone Ameliorates Experimental Autoimmune Encephalomyelitis Via Blocking Peroxynitrite Formation In Mouse Spinal Cord White Matter, Adam C. Vana, Shihe Li, Rachel Ribeiro, Flaubert Tchantchou, Yumin Zhang

Uniformed Services University of the Health Sciences

Inhibition of phospholipase A₂ (PLA₂) has recently been found to attenuate the pathogenesis of experimental autoimmune encephalomyelitis (EAE), a commonly used animal model of multiple sclerosis (MS). However, the protective mechanisms that underlie PLA₂ inhibition are still not well understood. In this study, we found that cytosolic PLA₂ (cPLA₂) was highly expressed in infiltrating lymphocytes and macrophages/microglia in mouse spinal cord white matter. Although cPLA₂ is also expressed in spinal cord neurons and oligodendrocytes, there were no differences observed in these cell types between EAE and control animals. Arachidonyl trifluoromethyl ketone (AACOCF3), …

Low-Dose Human Chorionic Gonadotropin May Improve In Vitro Fertilization Cycle Outcomes In Patients With Low Luteinizing Hormone Levels After Gonadotropin-Releasing Hormone Antagonist Administration, Anthony M. Propst, Micah J. Hill, Gordon Wright Bates, Michelle Palumbo, Anne K. Van Horne, Matthew G. Retzloff

Uniformed Services University of the Health Sciences

Objective: To evaluate the effect of low levels of endogenous luteinizing hormone (LH) and low-dose human chorionic gonadotropin (hCG) supplementation on in vitro fertilization (IVF) cycle outcomes in a gonadotropinreleasing hormone (GnRH) antagonist protocol.

Design: Retrospective study. Setting: Military medical center. Patient(s): General in vitro fertilization/embryo transfer (IVF-ET) population.

Intervention(s): Addition of low-dose urinary hCG to IVF stimulations using a recombinant follicle-stimulating hormone (FSH) and GnRH antagonist protocol. Main

Outcome Measure(s): Implantation and live-birth rates.

Result(s): As part of a larger cohort of 239 patients, 42 patients with LH levels ≤0.5 mIU/mL were evaluated. In the larger cohort, there were …