Open Access. Powered by Scholars. Published by Universities.®

Medicine and Health Sciences Commons™

Open Access. Powered by Scholars. Published by Universities.®

University of Nebraska - Lincoln

2002

Discipline
Keyword
Publication

Articles 31 - 60 of 88

Full-Text Articles in Medicine and Health Sciences

Pretrial Publicity And Civil Cases: A Two-Way Street?, Brian H. Bornstein, Brooke L. Whisenhunt, Robert J. Nemeth, Deborah L. Dunaway Feb 2002

Pretrial Publicity And Civil Cases: A Two-Way Street?, Brian H. Bornstein, Brooke L. Whisenhunt, Robert J. Nemeth, Deborah L. Dunaway

Department of Psychology: Faculty Publications

Published pretrial publicity (PTP) research has been conducted almost exclusively with criminal cases and has focused on PTP that is detrimental to the defense. The current research examined the effects of PTP in a civil case to determine if PTP can have a biasing effect against either the defendant or the plaintiff in civil litigation. In Experiment 1, participants exposed to PTP biased against the defendant were more likely to reach a liable verdict than participants who read a control article or PTP biased against the plaintiff. Experiment 2 demonstrated that a judicial admonition did not reduce the biasing effect …

Go to article

Improvement In Quantity And Quality Of Prevention Measurement Of Toddler Injuries And Parental Interventions, Lizette Peterson, David Dilillo, Terri Lewis, Kenneth Sher Feb 2002

Improvement In Quantity And Quality Of Prevention Measurement Of Toddler Injuries And Parental Interventions, Lizette Peterson, David Dilillo, Terri Lewis, Kenneth Sher

Department of Psychology: Faculty Publications

Injury is the leading killer of children in the United States, yet little research has focused on this vital subject. One of the distinct barriers to injury prevention is the absence of effective assessment devices. Epidemiological aspects of injury have been assessed, but these provide little information at a level sufficient to allow conclusions about potential behavioral prevention methods. This paper describes an alternative, the Participant Event Monitoring (PEM) system. In this paper, the PEM system is used to examine a sample of 170 toddlers (ages 18 to 36 months), over a 6-month period, resulting in data on over 4,200 …

Go to article

Osmosensitive Release Of Neurotransmitter Amino Acids: Relevance And Mechanisms, Herminia Pasantes-Morales, Rodrigo Franco, Lenin Ochoa, Benito Ordaz Feb 2002

Osmosensitive Release Of Neurotransmitter Amino Acids: Relevance And Mechanisms, Herminia Pasantes-Morales, Rodrigo Franco, Lenin Ochoa, Benito Ordaz

School of Veterinary and Biomedical Sciences: Faculty Publications

Hyposmolarity activates amino acid efflux as part of the corrective volume process in a variety of cells. This review discusses the mechanism of amino acid release in brain cells preparations. Results present evidence of substantial differences between the efflux of taurine and that of GABA and glu-tamate, which besides a possible role as osmolytes, have a main function as synaptic transmitters. The differences found concern the efflux time course, the sensitivity to Cl– channel blockers, the modulation by tyrosine kinases, the influence of PKC and the effect of cytoskeleton disruptive agents. While taurine efflux features fit well with the …

Go to article

A Gene Capable Of Blocking Apoptosis Can Substitute For The Herpes Simplex Virus Type 1 Latency-Associated Transcript Gene And Restore Wild-Type Reactivation Levels, Guey-Chuen Perng, Barak Maguen, Ling Jin, Kevin R. Mott, Nelson Osorio, Susan M. Slanina, Ada Yukht, Homayon Ghiasi, Anthony B. Nesburn, Melissa Inman, Gail A. Henderson, Clinton J. Jones, Steven L. Wechsler Feb 2002

A Gene Capable Of Blocking Apoptosis Can Substitute For The Herpes Simplex Virus Type 1 Latency-Associated Transcript Gene And Restore Wild-Type Reactivation Levels, Guey-Chuen Perng, Barak Maguen, Ling Jin, Kevin R. Mott, Nelson Osorio, Susan M. Slanina, Ada Yukht, Homayon Ghiasi, Anthony B. Nesburn, Melissa Inman, Gail A. Henderson, Clinton J. Jones, Steven L. Wechsler

School of Veterinary and Biomedical Sciences: Faculty Publications

After ocular herpes simplex virus type 1 (HSV-1) infection, the virus travels up axons and establishes a lifelong latent infection in neurons of the trigeminal ganglia. LAT (latency-associated transcript), the only known viral gene abundantly transcribed during HSV-1 neuronal latency, is required for high levels of reactivation. The LAT function responsible for this reactivation phenotype is not known. Recently, we showed that LAT can block programmed cell death (apoptosis) in neurons of the trigeminal ganglion in vivo and in tissue culture cells in vitro (G.-C. Perng et al., Science 287:1500–1503, 2000; M. Inman et al., J. Virol. 75:3636–3646, 2001). Consequently, …

Go to article

Child Sexual Abuse, Eugenia Hsu, Georganna Sedlar, Mary Fran Flood, David J. Hansen Jan 2002

Child Sexual Abuse, Eugenia Hsu, Georganna Sedlar, Mary Fran Flood, David J. Hansen

Department of Psychology: Faculty Publications

This chapter describes the case of two adolescent girls who were living in foster care with their maternal aunt and her family, the Kraller family. The girls and their aunt and uncle participated in Project SAFE, a university-based program for sexually abused children and their nonoffending caregivers, which is described in detail in the Course of Treatment section. The Kraller family was referred to Project SAFE by the local Child Advocacy Center. Miriam Kraller contacted the Child Advocacy Center when she learned that her two nieces (her younger sister’s daughters), Gina (age 14) and Suzy (age 13), had been sexually …

Go to article

Construction And Characterization Of A Chimeric Virus (Biv/Hiv-1) Carrying The Bovine Immunodeficiency Virus Gag-Pol Gene: Research Letters, Guomin Chen, Shuhui Wang, Kun Xiong, Jinzhong Wang, Tao Ye, Wenping Dong, Qi Wang, Qimin Chen, Yunqi Geng, Charles Wood, Yi Zeng Jan 2002

Construction And Characterization Of A Chimeric Virus (Biv/Hiv-1) Carrying The Bovine Immunodeficiency Virus Gag-Pol Gene: Research Letters, Guomin Chen, Shuhui Wang, Kun Xiong, Jinzhong Wang, Tao Ye, Wenping Dong, Qi Wang, Qimin Chen, Yunqi Geng, Charles Wood, Yi Zeng

Nebraska Center for Virology: Faculty Publications

HIV-1HXB2 5′LTR region, most of BIVR29 gag-pol segment and HIV-1HXB2 pol IN-3′LTR region were respectively amplified. A chimeric clone, designated as pHBIV3753, was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene.

Go to article

Using Controversial Mock Trials In “Psychology And Law” Courses: Suggestions From Participants, James L. Werth, James Harvey, Rebecca Mcnamara, Andrea Svoboda, Raina Gulbrandson, Jennifer Hendren, Tiffany Greedy, Christie Leybold Jan 2002

Using Controversial Mock Trials In “Psychology And Law” Courses: Suggestions From Participants, James L. Werth, James Harvey, Rebecca Mcnamara, Andrea Svoboda, Raina Gulbrandson, Jennifer Hendren, Tiffany Greedy, Christie Leybold

Department of Psychology: Faculty Publications

We discuss the use of a mock trial of the controversial Jack Kevorkian euthanasia case in an undergraduate psychology and law course. We provide an overview of the project, student feedback, and recommendations for improving the exercise. Based on feedback from students, we conclude that constructing a trial around a controversial topic with psychosocial issues as the foundation of a course can increase the utility of a mock trial and make the course a better learning experience.

Go to article

Department Of Veterinary And Biomedical Sciences: 2002 Annual Report Jan 2002

Department Of Veterinary And Biomedical Sciences: 2002 Annual Report

Veterinary and Biomedical Sciences -- Department Information and History

Foreword 2002
VBMS Faculty
VBMS Faculty and Staff, by Function and Unit
Honors. Awards and Recognitions. 2002
VBMS Committee Assignments. 2002
Faculty Profiles
VBMS Research Associates Profiles
VBMS Teaching Program
Great Plains Veterinary Educational Center (GPVEC)
VBMS Research Program
International Activities
Veterinary Extension Program
Nebraska Veterinary Diagnostic Laboratory Systems (NVDLS)
VBMS Grants and Contracts Program
Patents by VBMS Faculty in 2002
Publications by VBMS Faculty
Presentations by VBMS Faculty
Articles Regarding the Department
Selected Committees. Editorial and Other Appointments
Departmental Budget Summaries
Nebraska Agricultural Statistics

Go to article

Derivation Of Risk Based Wipe Surface Screening Levels For Industrial Scenarios, Lisa M. May, Bonnie Gaborek, Tony Pitrat, Laura Peters Jan 2002

Derivation Of Risk Based Wipe Surface Screening Levels For Industrial Scenarios, Lisa M. May, Bonnie Gaborek, Tony Pitrat, Laura Peters

Uniformed Services University of the Health Sciences

The environmental characterization of building interiors and other surfaces has generally been performed with wipe-sampling because it is a non-destructive technique. There is no consensus, however, as to the interpretation of the results of wipe-sampling. Specifically, there is not a standardized method to determine if chemicals found at sampled levels pose a threat to human health. A methodology was developed, based on acceptable health risk levels, to derive screening levels for evaluating wipe-sampling results pertaining to industrial scenarios. The methodology was based on the United States Environmental Protection Agency (USEPA) Region IX Preliminary Remediation Goal (PRG) approach; a multi-exposure methodology …

Go to article

The Saccharomyces Cerevisiae Ybr159w Gene Encodes The 3-Ketoreductase Of The Microsomal Fatty Acid Elongase, Gongshe Han, Ken Gable, Sepp D. Kohlwein, Frédéric Beaudoin, Johnathan A. Napier, Teresa M. Dunn Jan 2002

The Saccharomyces Cerevisiae Ybr159w Gene Encodes The 3-Ketoreductase Of The Microsomal Fatty Acid Elongase, Gongshe Han, Ken Gable, Sepp D. Kohlwein, Frédéric Beaudoin, Johnathan A. Napier, Teresa M. Dunn

Uniformed Services University of the Health Sciences

The YBR159w gene encodes the major 3-ketoreductase activity of the elongase system of enzymes required for very long-chain fatty acid (VLCFA) synthesis. Mutants lacking the YBR159w gene display many of the phenotypes that have previously been described for mutants with defects in fatty acid elongation. These phenotypes include reduced VLCFA synthesis, accumulation of high levels of dihydrosphingosine and phytosphingosine, and accumulation of medium-chain ceramides. In vitro elongation assays confirm that the ybr159Δ mutant is deficient in the reduction of the 3-ketoacyl intermediates of fatty acid elongation. The ybr159Δ mutant also displays reduced dehydration of the 3-OH acyl intermediates of fatty …

Go to article

Cell Surface-Localized Nucleolin Is A Eukaryotic Receptor For The Adhesin Intimin-Γ Of Enterohemorrhagic Escherichia Coli O157:H7, James F. Sinclair, Alison D. O'Brien Jan 2002

Cell Surface-Localized Nucleolin Is A Eukaryotic Receptor For The Adhesin Intimin-Γ Of Enterohemorrhagic Escherichia Coli O157:H7, James F. Sinclair, Alison D. O'Brien

Uniformed Services University of the Health Sciences

Intimin-γ is an outer membrane protein of enterohemorrhagic Escherichia coli (EHEC) O157:H7 that is required for the organism to adhere tightly to HEp-2 cells and to colonize experimental animals. Another EHEC O157:H7 protein, the Transferred intimin receptor (Tir), is considered the primary receptor for intimin-γ. Nevertheless, Tir-independent binding of intimin-γ to HEp-2 cells has been reported. This observation suggests the existence of a eukaryotic receptor(s) for intimin-γ. In this study, we sought to identify that receptor(s). First, we determined by equilibrium binding titration that the association of purified intimin-γ with HEp-2 cells was specific and consistent …

Go to article

Validation Of Monoclonal Antibody F99/97.6.1 For Immunohistochemical Staining Of Brain And Tonsil In Mule Deer (Odocoileus Hemionus) With Chronic Wasting Disease, T. R. Spraker, Katherine I. O'Rourke, A. Balachandran, R. R. Zink, B. A. Cummings, M. W. Miller, B. E. Powers Jan 2002

Validation Of Monoclonal Antibody F99/97.6.1 For Immunohistochemical Staining Of Brain And Tonsil In Mule Deer (Odocoileus Hemionus) With Chronic Wasting Disease, T. R. Spraker, Katherine I. O'Rourke, A. Balachandran, R. R. Zink, B. A. Cummings, M. W. Miller, B. E. Powers

Other Publications in Zoonotics and Wildlife Disease

A new monoclonal antibody (MAb), F99/97.6.1, that has been used to demonstrate scrapieassociated prion protein PrPSc in brain and lymphoid tissues of domestic sheep with scrapie was used in an immunohistochemistry assay for diagnosis of chronic wasting disease (CWD) in mule deer (Odocoileus hemionus). The MAb F99/97.6.1 immunohistochemistry assay was evaluated in brain and tonsil tissue from 100 mule deer that had spongiform encephalopathy compatible with CWD and from 1,050 mule deer outside the CWD-endemic area. This MAb demonstrated abnormal protease-resistant prion protein (PrPres) in brains of all of the 100 mule deer and in …

Go to article

Comparison Of Histological Lesions And Immunohistochemical Staining Of Proteinase-Resistant Prion Protein In A Naturally Occurring Spongiform Encephalopathy Of Free-Ranging Mule Deer (Odocoileus Hemionus) With Those Of Chronic Wasting Disease Of Captive Mule Deer, T. R. Spraker, R. R. Zink, B. A. Cummings, M. A. Wild, M. W. Miller, Katherine I. O'Rourke Jan 2002

Comparison Of Histological Lesions And Immunohistochemical Staining Of Proteinase-Resistant Prion Protein In A Naturally Occurring Spongiform Encephalopathy Of Free-Ranging Mule Deer (Odocoileus Hemionus) With Those Of Chronic Wasting Disease Of Captive Mule Deer, T. R. Spraker, R. R. Zink, B. A. Cummings, M. A. Wild, M. W. Miller, Katherine I. O'Rourke

Other Publications in Zoonotics and Wildlife Disease

In this investigation, the nature and distribution of histologic lesions and immunohistochemical staining (IHC) of a proteinase-resistant prion protein were compared in free-ranging mule deer (Odocoileus hemionus) dying of a naturally occurring spongiform encephalopathy (SE) and captive mule deer dying of chronic wasting disease (CWD). Sixteen free-ranging deer with SE, 12 free-ranging deer without SE, and 10 captive deer with CWD were examined at necropsy. Tissue sections were stained with hematoxylin and eosin, and duplicate sections were stained with a monoclonal antibody (F89/160.1.5). Histological lesions in the freeranging deer with SE and captive deer with CWD were found …

Go to article

Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran Jan 2002

Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention relates to methods and compositions for eliciting an immune response against bovine viral epitopes. The methods comprise combining at least one heat shock protein with at least one bovine viral epitope to form a purified epitope/heat shock protein complex and administration of an immune system stimulating amount of the purified epitope/heat shock protein complex. The compositions comprise, a purified epitope/heat shock protein complex comprising at least one bovine viral epitope complexed with at least one heat shock protein, and a pharmaceutically acceptable carrier, diluent or excipient.

Go to article

Dynamics Of Bovine Tuberculosis In Wild White-Tailed Deer In Michigan, Graham J. Hickling Jan 2002

Dynamics Of Bovine Tuberculosis In Wild White-Tailed Deer In Michigan, Graham J. Hickling

Michigan Bovine Tuberculosis Bibliography and Database

In mid-2001, the author of this report was invited to undertake an exploratory analysis of Michigan Department of Natural Resources’ data on bovine tuberculosis (TB) infection in wild white-tailed (Odocoileus virginianus) in the northeastern Lower Peninsula of the state. The aims of this analysis were:
• to quantify the geographic spread of tuberculous deer in Michigan;
• to investigate key factors influencing the prevalence of disease in the core of the infected area;
• to interpret the results in light of overseas experience with wildlife TB, with a view to
recommending how current management of the disease might …

Go to article

Environmental And Farm Management Factors Associated With Tuberculosis On Cattle Farms In Northeastern Michigan, John B. Kaneene, Colleen S. Bruning-Fann, Larry M. Granger, Roseann Miller, Barbara A. Porter-Spalding Jan 2002

Environmental And Farm Management Factors Associated With Tuberculosis On Cattle Farms In Northeastern Michigan, John B. Kaneene, Colleen S. Bruning-Fann, Larry M. Granger, Roseann Miller, Barbara A. Porter-Spalding

Michigan Bovine Tuberculosis Bibliography and Database

Objective—To identify major environmental and farm management factors associated with the occurrence of tuberculosis (TB) on cattle farms in northeastern Michigan.

Design—Case-control study.

Sample Population—17 cattle farms with infected cattle and 51 control farms.

Procedure—Each case farm (laboratory confirmed diagnosis of Mycobacterium bovis infection) was matched with 2 to 4 control farms (negative whole-herd test results within previous 12 months) on the basis of type of farm (dairy or beef) and location. Cattle farm data were collected from in-person interviews and mailed questionnaires. Wildlife TB data were gathered through state wildlife surveillance. Environmental data were gathered …

Go to article

Susceptibility Of Raccoons (Procyon Lotor) To Infection With Mycobacterium Bovis, Mitchell V. Palmer, W. Ray Waters, Diana L. Whipple Jan 2002

Susceptibility Of Raccoons (Procyon Lotor) To Infection With Mycobacterium Bovis, Mitchell V. Palmer, W. Ray Waters, Diana L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

Tuberculosis due to Mycobacterium bovis infection is endemic in white-tailed deer (Odocoileus virginianus) in the northeastern portion of the lower Michigan peninsula (USA). Various wild carnivores and omnivores, including raccoons (Procyon lotor), are infected with M. bovis within the endemic area. To investigate the pathogenesis of tuberculosis in raccoons and the likelihood of M. bovis transmission from infected raccoons to other susceptible hosts, we experimentally inoculated raccoons with single oral doses of M. bovis (ranging from 30 to 1.7 x 105 colony forming units [CFU]), five daily oral doses of M. bovis (ranging from 10 …

Go to article

Lesion Development In White-Tailed Deer (Odocoileus Virginianus) Experimentally Infected With Mycobacterium Bovis, M. V. Palmer, W. R. Waters, D. L. Whipple Jan 2002

Lesion Development In White-Tailed Deer (Odocoileus Virginianus) Experimentally Infected With Mycobacterium Bovis, M. V. Palmer, W. R. Waters, D. L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

The recent discovery of tuberculosis in free-living white-tailed deer in northeastern Michigan underscores the need for increased understanding of the pathogenesis of tuberculosis in wildlife species. To investigate lesion development in white-tailed deer, 32 deer were experimentally infected by intratonsilar instillation of 300 colony-forming units of Mycobacterium bovis. Three deer each were euthanatized and examined at days 15, 28, 42, and 56 after inoculation, and five deer each were euthanatized and examined at days 89, 180, 262, and 328 after inoculation. Microscopic lesions first were seen in the medial retropharyngeal lymph node and lung 28 and 42 days after …

Go to article

Nitric Oxide Production As An Indication Of Mycobacterium Bovis Infection In White-Tailed Deer (Odocoileus Virginianus), W. R. Waters, M. V. Palmer, R. E. Sacco, D. L. Whipple Jan 2002

Nitric Oxide Production As An Indication Of Mycobacterium Bovis Infection In White-Tailed Deer (Odocoileus Virginianus), W. R. Waters, M. V. Palmer, R. E. Sacco, D. L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

White-tailed deer ( Odocoileus virginianus ) are reservoirs for Mycobacterium bovis in northeast Michigan, USA. Production of nitric oxide (NO) by activated macrophages is a potent mechanism of mycobacterial killing. The capacity of macrophages to produce NO, however, varies among mammalian species. The objective of this study was to determine if mononuclear cells from white-tailed deer produce nitrite as an indication of NO production and, if so, is NO produced in response to stimulation with M. bovis antigens. Supernatants were harvested from adherent peripheral blood mononuclear cell (PBMC) cultures that had been stimulated with either Mannheimia haemolytica lipopolysaccharide (LPS) or …

Go to article

Mycobacterium Bovis–Infected White-Tailed Deer (Odocoileus Virginianus): Detection Of Immunoglobulin Specific To Crude Mycobacterial Antigens By Elisa, W. Ray Waters, Mitchell V. Palmer, Diana L. Whipple Jan 2002

Mycobacterium Bovis–Infected White-Tailed Deer (Odocoileus Virginianus): Detection Of Immunoglobulin Specific To Crude Mycobacterial Antigens By Elisa, W. Ray Waters, Mitchell V. Palmer, Diana L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

White-tailed deer (Odocoileus virginianus) have recently emerged as a source of Mycobacterium bovis infection for cattle within North America. The objective of this study was to evaluate the antibody response of M. bovis–infected deer to crude mycobacterial antigens. Deer were experimentally inoculated with M. bovis strain 1315 either by intratonsilar instillation or by exposure to M. bovis–infected (i.e., in contact) deer. To determine the time course of the response, including the effects of antigen administration for comparative cervical skin testing, serum was collected periodically and evaluated by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin (i.e., IgG heavy …

Go to article

Bovine Tuberculosis In Michigan Wildlife And Livestock, Stephen M. Schmitt, Daniel J. O'Brien, Colleen S. Brunning-Fann, Scott D. Fitzgerald Jan 2002

Bovine Tuberculosis In Michigan Wildlife And Livestock, Stephen M. Schmitt, Daniel J. O'Brien, Colleen S. Brunning-Fann, Scott D. Fitzgerald

Michigan Bovine Tuberculosis Bibliography and Database

Since 1994, the state of Michigan has recognized a problem with bovine tuberculosis (TB), caused by Mycobacterium bovis, in wild white-tailed deer from a 12-county area in northeastern Lower Michigan. A total of 65,000 free-ranging deer have been tested, and 340 have been found to be positive for M. bovis. The disease has been found in other wildlife species, and, in 1998, in domestic cattle, where to date 13 beef cattle and 2 dairy cattle herds have been diagnosed with bovine TB. Unfortunately, the situation is unique in that there have never been reports of self-sustaining bovine TB …

Go to article

Influence Of Protein Tyrosine Kinases On Cell Volume Change-Induced Taurine Release, Herminia Pasantes-Morales, Rodrigo Franco Jan 2002

Influence Of Protein Tyrosine Kinases On Cell Volume Change-Induced Taurine Release, Herminia Pasantes-Morales, Rodrigo Franco

School of Veterinary and Biomedical Sciences: Faculty Publications

Taurine efflux occurs in association with cell swelling in both hyposmotic and isosmotic conditions and during cell shrinkage in apoptotic death. Release occurs through a leak pathway, is largely Ca2+-independent and is sensitive to Cl– channel blockers. Taurine efflux elicited by hyposmolarity is reduced or suppressed by tyrosine kinase blockers and increased by tyrosine phosphatase inhibitors. The specific kinases involved are still unknown and may be different in the various cell types. Non-receptor and scr-related protein kinases have been identified in some cells as elements that directly phosphorylate the taurine efflux pathway. Possible tyrosine kinase targets are …

Go to article

Mechanisms Counteracting Swelling In Brain Cells During Hyponatremia, Herminia Pasantes-Morales, Rodrigo Franco, Benito Ordaz, Lenin D. Ochoa Jan 2002

Mechanisms Counteracting Swelling In Brain Cells During Hyponatremia, Herminia Pasantes-Morales, Rodrigo Franco, Benito Ordaz, Lenin D. Ochoa

School of Veterinary and Biomedical Sciences: Faculty Publications

Water gain in the brain consequent to hyponatremia is counteracted by mechanisms that initially include a compensatory displacement of liquid from the interstitial space to cerebrospinal fluid and systemic circulation and subsequently an active reduction in cell water accomplished by extrusion of intracellular osmolytes to reach osmotic equilibrium. Potassium (K+), chloride (Cl–), amino acids, polyalcohols, and methylamines all contribute to volume regulation, with a major contribution of ions at the early phase and of organic osmolytes at the late phase of the regulatory process. Experimental models in vitro show that osmolyte fluxes occur via leak pathways …

Go to article

Mycobacterium Smegmatis D-Alanine Racemase Mutants Are Not Dependent On D-Alanine For Growth, Ofelia Chacon, Zhengyu Feng, N. Beth Harris , Nancy E. Caceres, Garry Adams, Raul G. Barletta Jan 2002

Mycobacterium Smegmatis D-Alanine Racemase Mutants Are Not Dependent On D-Alanine For Growth, Ofelia Chacon, Zhengyu Feng, N. Beth Harris , Nancy E. Caceres, Garry Adams, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium smegmatis is a fast-growing nonpathogenic species particularly useful in studying basic cellular processes of relevance to pathogenic mycobacteria. This study focused on the D-alanine racemase gene (alrA), which is involved in the synthesis of D-alanine, a basic component of peptidoglycan that forms the backbone of the cell wall. M. smegmatis alrA null mutants were generated by homologous recombination using a kanamycin resistance marker for insertional inactivation. Mutants were selected on Middlebrook medium supplemented with 50 mM D-alanine and 20 μg of kanamycin per ml. These mutants were also able to grow in standard and minimal media without …

Go to article

Prosocial Development In Early Adulthood: A Longitudinal Study, Nancy Eisenberg, Ivanna K. Guthrie, Amanda Cumberland, Bridget C. Murphy, Stephanie A. Shepard, Qing Zhou, Gustavo Carlo Jan 2002

Prosocial Development In Early Adulthood: A Longitudinal Study, Nancy Eisenberg, Ivanna K. Guthrie, Amanda Cumberland, Bridget C. Murphy, Stephanie A. Shepard, Qing Zhou, Gustavo Carlo

Department of Psychology: Faculty Publications

Consistency of measures of a prosocial personality and prosocial moral judgment over time, and the interrelations among them, were examined. Participants’ and friends’ reports of prosocial characteristics were obtained at ages 21– 22, 23–24, and 25–26 years. In addition, participants’ prosocial judgment was assessed with interviews and with an objective measure of prosocial moral reasoning at several ages. Reports of prosocial behavior and empathy-related responding in childhood and observations of prosocial behavior in preschool also were obtained. There was interindividual consistency in prosocial dispositions, and prosocial dispositions in adulthood related to empathy/sympathy and prosocial behavior at much younger ages. Interview …

Go to article

Commentary And Reply To: Would Smokers With Schizophrenia Benefit From A More Flexible Approach To Smoking Treatment?, Tony George, Jennifer Vessicchio, David G. Gilbert, John R. Hughes, Dennis E. Mcchargue, Suzy B. Gulliver, Brian Hitsman Jan 2002

Commentary And Reply To: Would Smokers With Schizophrenia Benefit From A More Flexible Approach To Smoking Treatment?, Tony George, Jennifer Vessicchio, David G. Gilbert, John R. Hughes, Dennis E. Mcchargue, Suzy B. Gulliver, Brian Hitsman

Department of Psychology: Faculty Publications

Treating Tobacco Addiction in Schizophrenia: Where do we go From Here?
Yes! Smokers with Schizophrenia will Benefit from More Flexible Treatment Approaches
Rigidity in Measures of Smoking Cessation
A Reply to the Commentaries on Schizophrenia and Smoking Treatment: More Research is Needed

Go to article

Agency In The Life Course: Concepts And Processes, Lisa J. Crockett Jan 2002

Agency In The Life Course: Concepts And Processes, Lisa J. Crockett

Department of Psychology: Faculty Publications

The capacity of humans to influence their lives has long been a theme within Western literature, philosophy, and psychology. In recent years, the theme of human agency has crystallized in the psychological literature in the concepts of control, self-regulation, and self-efficacy. As a result, considerable attention has been devoted to the psychological processes through which control is exerted, such as cognitive appraisals, goal setting, and planning, as well as control beliefs and the potentially debilitating effects of loss of control (e.g., Bandura, 1997; Boekaerts, Pintrich, & Zeidner, 2000; Carver & Scheier, 1998; Seligman, 1975). Although this body of work has …

Go to article

Antisocial Alcoholism And Serotonin-Related Polymorphisms: Association Tests, Elizabeth M. Hill, Scott F. Stoltenberg, Katherine Harris Bullard, Sheng Li, Robert A. Zucker, Marget Burmeister Jan 2002

Antisocial Alcoholism And Serotonin-Related Polymorphisms: Association Tests, Elizabeth M. Hill, Scott F. Stoltenberg, Katherine Harris Bullard, Sheng Li, Robert A. Zucker, Marget Burmeister

Department of Psychology: Faculty Publications

Central serotonin dysfunction appears to be related to a subtype of alcoholism with antisocial impulsive features (type II; antisocial alcoholism). The serotonergic deficit may be associated with greater impulsivity, which in turn facilitates both alcohol dependence and antisocial behavior. The present study tested association of antisocial impulsive alcoholism with candidate genes related to serotonergic neurotransmission, using families. Eight markers were assayed using polymerase chain reaction: tryptophan hydroxylase (intron 7), the serotonin transporter SLC6A4 (VNTR 9/12), HTTLPR, the three serotonin receptor types HTR1B (G861C), HTR2A (T102C) and HTR2C (Cys23Ser), monoamine oxidase A (T1460C), and (CA)n. Eligible probands had early …

Go to article

The Effects Of Depression And Anxiety On Memory Performance, Ali H. Kizilbash, Rodney D. Vanderploeg, Glenn Curtiss Jan 2002

The Effects Of Depression And Anxiety On Memory Performance, Ali H. Kizilbash, Rodney D. Vanderploeg, Glenn Curtiss

Public Health Resources

The effects of depression and anxiety, as assessed by MMPI D and Pt scales, on memory performance was examined in 3999 veterans who completed the California Verbal Learning Test (CVLT). Depressive symptoms (without anxiety) had an adverse effect on immediate recall of new information and the total amount (but not rate) of acquisition; however, retrieval and retention were unaffected. On the other hand, high levels of anxiety did not have significant detrimental effects on any aspect of memory functioning assessed including immediate recall, total amount acquired, retention, and retrieval of novel information. However, when depression was compounded by anxiety, there …

Go to article

Chronic Ethanol Administration Decreases The Ligand Binding Properties And The Cellular Content Of The Mannose 6-Phosphatelinsulin-Like Growth Gactor Ii Receptor In Rat Hepatocytes, James Haorah, Daniel L. Mcvicker, James C. Byrd, Richard G. Macdonald, Terrence M. Donohue, Jr. Jan 2002

Chronic Ethanol Administration Decreases The Ligand Binding Properties And The Cellular Content Of The Mannose 6-Phosphatelinsulin-Like Growth Gactor Ii Receptor In Rat Hepatocytes, James Haorah, Daniel L. Mcvicker, James C. Byrd, Richard G. Macdonald, Terrence M. Donohue, Jr.

Public Health Resources

We have shown previously that chronic ethanol administration impairs the maturation of lysosomal enzymes in rat hepatocytes. The mannose 6-phosphate/insulin-like growth factor I1 receptor (M6PLGF-IIR) is a protein that facilitates the transport of lysosomal enzymes into the lysosome. Therefore, we examined whether ethanol consumption altered the ligand binding properties and the cellular content of M6P/IGF-IIR. Rats were pair-fed liquid diets containing either ethanol (36% of calories) or isocaloric maltose-dextrin for either 1 week or 5-7 weeks. Hepatocytes prepared from these animals were examined for receptor-ligand binding and receptor content. One week of ethanol feeding had no significant effect on ligand …

Go to article

First Prev Next Last