Medicine and Health Sciences Commons^™

Identification Of Four Complementary Rna Species In Akabane Virus-Infected Cells, Asit K. Pattnaik, G. Abraham

School of Veterinary and Biomedical Sciences: Faculty Publications

The analysis of RNA extracted from purified Akabane virus demonstrated the presence of three size classes of single-stranded RNAs with sedimentation coefficients of 31S (large, L), 26S (medium, M), and 13S (small, S). Molecular weights of these RNA species were estimated to be 2.15 x 10⁶,1.5 X 10⁶, and 0.48 x 10⁶ for the L, M, and S RNAs, respectively. Hybridization analysis involving viral genomic RNA and RNA from virus-infected cells resulted in the identification of four virus-specific cRNA species in infected cells. These cRNAs were found to be nonpolyadenylated by their inability to bind …

Field Technique For The Identification Of Deer Blood, David W. Oates, Carol A. Jochum, Kenneth A. Pearson, Cathy A. Hoilien

Nebraska Game and Parks Commission: Staff Research Publications

A latex suspension sensitized with deer antiserum has been prepared, placed on plastic cards, dried, and packaged for field use. The product was tested against bloodstains from 22 species (including Homo sapiens). Strong agglutination reactions occurred only with bloodstains from deer and elk.

Escherichia Coli Strains Producing Streptococcus Mutans Proteins Responsible For Colonization And Virulence, Roy Curtiss Iii, Robert G. Holt, Raul G. Barletta, James P. Robeson, Shigeno Saito

School of Veterinary and Biomedical Sciences: Faculty Publications

Streptococcus mutans is a principal etiologic agent of dental caries and is likely one of the most ubiquitous bacterial infectious disease agents worldwide. The ability of S. mutans to colonize the oral cavity is due to sucroseindependent and sucrose-dependent adherence to the pellicle-coated tooth surface with glucan facilitated aggregation between cells to result in plaque. Cariogenicity is then caused by the ability of S. mutans in plaque to metabolize free sugars and both extra and intracellular complex carbohydrates to yield predominantly lactic acid. The S. mutans gene products that contribute to colonizing ability and thus virulence include glucosyltransferases, glucanbinding proteins, …

A Balb/C Congenic Strain Of Mice That Carries A Genetic Locus (ItyR) Controlling Resistance To Intracellular Parasites, Michael Potter, Alison D. O'Brien, Emil Skamene, Philippe Gros, Adrien Forget, Patricia A. L. Kongshavn, Judith S. Wax

Uniformed Services University of the Health Sciences

BALB/c.DBA/2 Idh-1^b-Ity^r-Pep-3^b congenic mice were developed by introgressively backcrossing the Idh-1^b and Pep-3^b markers of DBA/2 mice onto the BALB/cπ mice. This introduced a 30-centimorgan chromosome 1 segment of DBA/2 chromatin that contained the Ity^r gene. BALB/c.DBA/2 Idh-1^b-Ity^r-Pep-3^b mice were resistant to in vivo infections by Salmonella typhimurium, Mycobacterium bovis, and Leishmania donovani.

Expression Of A Streptococcus Mutans Glucosyltransferase Gene In Escherichia Coli, James P. Robeson, Raul G. Barletta, Roy Curtiss Iii

School of Veterinary and Biomedical Sciences: Faculty Publications

Chromosomal DNA from Streptococcus mutans strain UAB90 (serotype c) was cloned into Escherichia coli K-12. The clone bank was screened for any sucrosehydrolyzing activity by selection for growth on raffinose in the presence of isopropyl- β-D-thiogalactoside. A clone expressing an S. mutans glucosyltransferase was identified. The S. mutans DNA encoding this enzyme is a 1.73-kilobase fragment cloned into the HindIII site of plasmid pBR322. We designated the gene gtfA. The plasmid-encoded gtfA enzyme, a 55,000-molecular-weight protein, is synthesized at 40% the level of pBR322-encoded β-lactamase in E. coli minicells. Using sucrose as substrate, the gtfA enzyme catalyzes …

31P Nmr Study Of Erythrocytes From A Patient With Hereditary Pyrimidine-5'-Nucleotidase Deficiency, M. S. Swanson, C. R. Angle, S. J. Stohs, S. T. Wu, J. M. Salhany, R. S. Elliot, R. S. Markin

Public Health Resources

The composition of phosphate metabolites and the intracellular pH in erythrocytes from a patient with hereditary pyrimidine-5'-nucleotidase deficiency were examined using ³¹P NMR spectroscopy. Several resonances were identified in spectra from intact cells and from extracts. The 2,3-bisphosphoglycerate line intensities were normal but the NTP resonances were about twice normal due to the presence of millimolar quantities of pyrimidine phosphates. Several intense resonances were also observed in the diphosphodiester region of the spectrum. One compound contributing to these lines has been identified as cytidine diphosphocholine. The resonances of NTPs were in a position indicating that the additional triphosphates were …

Purification And Characterization Of A Shigella Dysenteriae 1- Like Toxin Produced By Escherichia Coli, Alison D. O'Brien, Gerald D. Laveck

Uniformed Services University of the Health Sciences

A toxin from an enteropathogenic strain of Escherichia coli (E. coli H30) was purified to apparent homogeneity from cell lysates. The steps used to isolate the E. coli H30 toxin included French pressure-cell disruption of bacteria grown in iron-depleted media, Affi-Gel Blue chromatography, chromatofocusing, and anti- Shiga toxin affinity chromatography. The mobilities of the subunits of radioiodinated E. coli H30 toxin and Shiga toxin observed after the two toxins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis were identical. In the absence of 2-mercaptoethanol, a narrow band was seen at M_r 31,500 (±1,000), and a wide heavy band …

United States Naval Medical Research Unit Number Three, Cairo, Egypt, Medical Zoology Department Translation List, Harry Hoogstraal

United States Naval Medical Research Unit 3: Publications

List of translations of medical parasitology and medical entomology papers prepared by the United States Naval Medical Research Unit Number 3 in Cairo, Egypt. The papers were translated from numerous languages, such as Russian (most were translated from Russian), Bulgarian, Chinese, Czech, Finnish, French, German, and Japanese, into English. The publication dates of the original papers ranged from about 1915 to 1983. Harry Hoogstraal translated some or all of the papers.

Namru-3 Translations Of Parasite Literature From Russian, French, Japanese, Etc. Into English (1915-1983) T1601-T1700, United States Naval Medical Research Unit Number Three, Cairo, Medical Zoology Department

United States Naval Medical Research Unit 3: Publications

T1603. TRANSLATION FROM RUSSIAN. SELIVANOV, Ya. M. and POLYAKOVA, A. N. (1981). Technic of preparing and producing experimental series of immunoglobulin erythrocytic polytype diagnosticums to certain arboviruses for the indirect hemagglutination test. Sborn. Trud. Inst. Virus. imeni D. I. Ivanovsky, Akad. Med. Nauk SSSR, pp. 145-151.

T1604. TRANSLATION FROM RUSSIAN. ROSLAYA, I. G., DEMENEV, V. A., PRYGOVA, A. I., ROSLYAKOV, G. E. and VERETA, L. A. (1981). Certain questions of ecology of tickborne encephalitis group viruses in the lower Pre-Amur'ye. Sborn. Trud. Inst. Virus. imeni D. I. Ivanovsky, Akad. Med. Nauk SSSR, pp. 90-94.

T1605. TRANSLATION FROM RUSSIAN. EMEL'YANOVA. N. …