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Full-Text Articles in Medicine and Health Sciences
A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi
A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi
Turkish Journal of Veterinary & Animal Sciences
Avian colibacillosis (AC) is frequently reported from poultry farms in the west of Iran. The aim of the present survey was to evaluate the phylogeny of Escherichia coli isolated from AC through the Clermont triplex protocol and gyrA gene sequencing. One hundred and fifty fresh carcasses suspected of having died from colibacillosis were sampled. The specimens were examined for E. coli isolation and identification. The extracted DNA repertoire of the isolates was subjected to phylogenetic analysis using Clermont triplex PCR. A DNA fragment of 1.8 kbp of the gyrA gene was amplified from a representative of each phylogroup or subgroup …
Antimicrobial Resistance And Phenotypic And Molecular Detection Of Extended-Spectrum Ss-Lactamases Among Extraintestinal Escherichia Coli Isolated From Pneumonic And Septicemic Sheep And Goats In Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Jyoti Kumar, Shivendra Kumar Dixit, Rajendra Kumar Meena, Bhupendra Nath Tripathi
Antimicrobial Resistance And Phenotypic And Molecular Detection Of Extended-Spectrum Ss-Lactamases Among Extraintestinal Escherichia Coli Isolated From Pneumonic And Septicemic Sheep And Goats In Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Jyoti Kumar, Shivendra Kumar Dixit, Rajendra Kumar Meena, Bhupendra Nath Tripathi
Turkish Journal of Veterinary & Animal Sciences
The present study was planned to isolate Escherichia coli from pneumonic and septicemic sheep and goats and to determine antimicrobial resistance (AMR) patterns of the isolates and evaluate them for the presence of extended-spectrum ß-lactamase (ESBL) using the phenotypic and molecular methods. Pneumonic lung tissues and heart blood samples were collected by performing necropsy on sheep (n = 96) and goats (n = 08). The samples were processed and used for bacterial isolation. E. coli isolates (n = 58) including 53 from 34 sheep and 5 from 3 goats were recovered and identified by the cultural and biochemical characteristics and …
Virulence Factors And Antimicrobial Resistance In Uropathogenic Escherichiacoli Strains Isolated From Cystitis And Pyelonephritis, Hosseinali Ghazvini, Keyvan Taheri, Elahe Edalati, Ali Miri, Mansour Sedighi, Shiva Mirkalantari
Virulence Factors And Antimicrobial Resistance In Uropathogenic Escherichiacoli Strains Isolated From Cystitis And Pyelonephritis, Hosseinali Ghazvini, Keyvan Taheri, Elahe Edalati, Ali Miri, Mansour Sedighi, Shiva Mirkalantari
Turkish Journal of Medical Sciences
Background/aim: The aim of this study was to investigate the prevalence of virulence genes as well as patterns of antibiotic resistance in cystitis and pyelonephritis uropathogenic Escherichia coli (UPEC) isolates. Materials and methods: Two hundred UPEC isolates were collected from hospitalized patients with pyelonephritis (n = 50) and cystitis (n = 150) in Shafa Hospital in Iran. Antimicrobial susceptibility and ESBL production were determined with confirmatory tests. Polymerase chain reaction assay was performed to determine the prevalence of virulence genes in UPEC strains. Results: Of a total 200 UPEC isolates, the highest and lowest resistance rates to antibiotics were for …
Biofilm Formation In Uropathogenic Escherichia Coli: Association With Adhesion Factor Genes, Hojjatolah Zamani, Ali Salehzadeh
Biofilm Formation In Uropathogenic Escherichia Coli: Association With Adhesion Factor Genes, Hojjatolah Zamani, Ali Salehzadeh
Turkish Journal of Medical Sciences
Background/aim: Biofilm formation is a major determinant factor in the development of urinary tract infections (UTIs) by uropathogenic Escherichia coli (UPEC). Several adhesion factors are involved in attachment of bacterial cells to the urinary tract and biofilm development. Materials and methods: The possible relationship between different adhesion factor genes (AFGs) and biofilm formation among UPEC isolates was investigated. Prevalence of different AFGs including fimA, fimH, papAH, papC, papEF, sfa-S, foc/G, and bmaE and their association to biofilm formation were investigated. Phenotypic expression of type 1 and P fimbriae was also investigated. Results: Our results showed that 84% of UPEC were …
Fecal Carriage Of Extended-Spectrum Beta-Lactamase-Producing Escherichia Coli And Klebsiella Spp. In A Turkish Community, Derya Çakir Erdoğan, Füsun Cömert, Eli̇f Aktaş Sepetci̇, Fürüzan Köktürk, Canan Külah
Fecal Carriage Of Extended-Spectrum Beta-Lactamase-Producing Escherichia Coli And Klebsiella Spp. In A Turkish Community, Derya Çakir Erdoğan, Füsun Cömert, Eli̇f Aktaş Sepetci̇, Fürüzan Köktürk, Canan Külah
Turkish Journal of Medical Sciences
Background/aim: The aim of this study was to determine the prevalence of fecal carriage of extended-spectrum beta-lactamase (ESBL)-producing bacteria, enzyme types, and risk factors affecting colonization. Materials and methods: A total of 576 stool samples from outpatients were examined between October 2012 and May 2013. Screening was done with selective EMB plates. ESBL were detected by double-disk synergy and confirmed agar strip gradient methods. Enzyme types were determined by PCR. Results: The prevalence of fecal carriage was found as 30% (173 of 576). Recent use of antibiotics, hospitalization and surgical operation, diabetes, crowded household populations, and old age were associated …
Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey, Ni̇lgün Ünal, Alper Karagöz, Şi̇nasi̇ Aşkar, Zahi̇de Di̇li̇k, Buket Yurteri̇
Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey, Ni̇lgün Ünal, Alper Karagöz, Şi̇nasi̇ Aşkar, Zahi̇de Di̇li̇k, Buket Yurteri̇
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broiler slaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified using a BBL Crystal Enteric/Nonfermenter ID Kit (Becton Dickinson, USA) and ESBL production was confirmed using an ESBL phenotypic confirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis (PFGE) was performed with XbaI for the clonal typing of ESBL-producing E. coli isolates. While 33 phenotypic …
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Turkish Journal of Veterinary & Animal Sciences
Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …
Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters, Alma Carrascosa, Alejandro De La Peña, Lilia Gutierrez, Hector Sumano
Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters, Alma Carrascosa, Alejandro De La Peña, Lilia Gutierrez, Hector Sumano
Turkish Journal of Veterinary & Animal Sciences
Pharmacokinetics (PK), pharmacodynamics (PD), and PK/PD ratios of a new solvate of enrofloxacin (enrofloxacin hydrochloride-dihydrate; enro-C), were studied in hamsters. Enrofloxacin from Baytril1397904493 5% (enroR) served as the reference preparation. Two groups of 60 Syrian golden hamsters were intramuscularly injected individually with 10 mg/kg of enroR or with enro-C. Tissue and serum samples were obtained for 72 h; enrofloxacin concentrations were determined by HPLC with UV detection. Ninety percent minimum inhibitory concentrations (MIC90) were determined for strains of methicillin-resistant Staphylococcus aureus, Leptospira interrogans, and Escherichia coli. All PK variables were statistically different between groups (P < 0.01). CMAX of enrofloxacin for enro-C was 17.3 μg/mL and it was 2.6 μg/mL for enroR. AUC was considerably higher for enro-C (459.2 μg/mL h vs. 19.9 μg/mL h). There were no statistically significant differences in MIC90 values between enroR and enro-C. Tissue concentrations of enro-C in all cases were higher and remained above the MIC for longer periods than those of enroR. Relevant PK/PD ratios for enrofloxacin (AUC/MIC ? 125 and CMAX > MIC = 10-12) are consequently …
Effect Of Melatonin On Contractile Activity In Intraurethrally Instilled E. Coli-Induced Acute Prostatitis Rat Model, Bakhtiyar Huseynov, Nadi̇re Eser, İbrahi̇m Ati̇lla Aridoğan, Şeyda Erdoğan, Yusuf Kenan Dağlioğlu, Hacer Si̇nem Büyüknacar, Fati̇h Köksal, Cemi̇l Göçmen
Effect Of Melatonin On Contractile Activity In Intraurethrally Instilled E. Coli-Induced Acute Prostatitis Rat Model, Bakhtiyar Huseynov, Nadi̇re Eser, İbrahi̇m Ati̇lla Aridoğan, Şeyda Erdoğan, Yusuf Kenan Dağlioğlu, Hacer Si̇nem Büyüknacar, Fati̇h Köksal, Cemi̇l Göçmen
Turkish Journal of Medical Sciences
To investigate whether prostatic inflammation affects the contractile responses of prostate tissue and then whether melatonin has any restorative effect on the contractile responses in a rat model of bacterial prostatitis at early stages. Materials and methods: We evaluated the nerve-evoked, adrenergic agonist-induced or cholinergic agonist-induced contractions of isolated prostate tissue at 24 and 72 h after intraurethrally instilled Escherichia coli-treatment. We also analyzed the histological changes in the prostate. Secondarily, we investigated the effect of melatonin pretreatment (7 days, 10 mg kg^{-1} day^{-1}) on the contractile responses and the histopathologic changes. Results: The degrees of acute inflammatory cell infiltration, …
Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis
Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis
Turkish Journal of Veterinary & Animal Sciences
A 4-year-old Chios ewe was submitted to the Clinic of Farm Animals (School of Veterinary Medicine, Aristotle University of Thessaloniki). The animal had delivered 2 healthy lambs, 2 days earlier, and was inappetent, depressed, and reluctant to move, while it had tachycardia and tachypnea. The cervix was opened at a dilation of 1 finger in diameter and fetid fetal membranes expelled from the vagina. Serum biochemistry revealed renal and liver disease, while ultrasonography revealed uterine dilatation. Laparotomy revealed a partly autolysed embryo in the uterus and gangrenous metritis. Clostridium perfringens and Escherichia coli were isolated in bacterial cultures from the …
Histopathological Effects Of S. Xylosus Peptidoglycan In Comparison To E. Coli Lipopolysaccharide In The Urinary Tract Of Mice, Harith Jabbar Fahad Al-Mathkhury, May Talib Flaih, Hiba Khalil Tawfiq
Histopathological Effects Of S. Xylosus Peptidoglycan In Comparison To E. Coli Lipopolysaccharide In The Urinary Tract Of Mice, Harith Jabbar Fahad Al-Mathkhury, May Talib Flaih, Hiba Khalil Tawfiq
Turkish Journal of Medical Sciences
The present work, among other previous studies done in our lab, aimed to highlight the histopathological effect of S. xylosus peptidoglycan in comparison to LPS of E. coli. Materials and methods: One hundred and fifty urine specimens were collected from urinary tract infection patients visiting Baghdad hospitals. The histopathological effects of S. xylosus S24 peptidoglycan was studied in the urinary tract of female mice by injecting 5 animal groups at the following concentrations: 1000, 2000, 3000, 4000, and 5000 µg/mL. Another 5 groups were injected with 10, 25, 50, 75, and 100 ng/mL of E. coli (serotype 0128:B12) LPS. Results: …
Influence Of Cyclosporine A And Quercetine On Mic Values Of Danofloxacin Mesylate In Escherichia Coli Strains Isolated From Poultry, Nikolina Velizarova Rusenova, Aneliya Milanova Haritova, Parvan Rusenov Parvanov
Influence Of Cyclosporine A And Quercetine On Mic Values Of Danofloxacin Mesylate In Escherichia Coli Strains Isolated From Poultry, Nikolina Velizarova Rusenova, Aneliya Milanova Haritova, Parvan Rusenov Parvanov
Turkish Journal of Veterinary & Animal Sciences
A reference strain Escherichia coli ATCC 25922, sensitive and resistant strains of E. coli, isolated from chickens with colibacillosis were tested. The aim of the present study was to determine the effect of modulators of ABC efflux transporters in eukaryotic cells cyclosporine A and quercetine on MIC values of danofloxacin mesylate. MIC values of danofloxacine mesylate against the chosen strains were determined with or without modulators at concentrations of 25 \mu M, 5 \mu M, 1 \mu M, and 0.2 \mu M by macro broth dilution method. Results show that in the presence of cyclosporine A, MIC values decreased from …
Survival Of Certain Nosocomial Infectious Agents On The Surfaces Of Various Covering Materials, Hali̇l Yazgi, M. Hami̇dullah Uyanik, Mustafa Ertek, Ayşe Esi̇n Aktaş, Hakan İgan, Ahmet Ayyildiz
Survival Of Certain Nosocomial Infectious Agents On The Surfaces Of Various Covering Materials, Hali̇l Yazgi, M. Hami̇dullah Uyanik, Mustafa Ertek, Ayşe Esi̇n Aktaş, Hakan İgan, Ahmet Ayyildiz
Turkish Journal of Medical Sciences
Aim: The aim of the present study was to investigate the survival time of some nosocomial infectious agents on the surface of various covering materials. Materials and Methods: Four kinds of flooring materials (vinyl and ceramic tile as floor covering material; and laminate and inox sheet as bench covering material) were experimentally contaminated with Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and vancomycin-resistant (VR) Enterococcus faecalis, which were frequently encountered in nosocomial infections. The survival periods of representative agents on the tested flooring materials were detected by culturing from the surfaces. Results: It was found that the survival periods were shorter …
Susceptibility Of Extended-Spectrum Beta-Lactamase-Producing Escherichia Coli Urine Isolates To Fosfomycin, Ciprofloxacin, Amikacin And Trimethoprim-Sulfamethoxazole, Hüsnü Pullukçu, Şöhret Aydemi̇r, Meltem Işikgöz Taşbakan, Feri̇ha Çi̇lli̇, Alper Tünger, Sercan Ulusoy
Susceptibility Of Extended-Spectrum Beta-Lactamase-Producing Escherichia Coli Urine Isolates To Fosfomycin, Ciprofloxacin, Amikacin And Trimethoprim-Sulfamethoxazole, Hüsnü Pullukçu, Şöhret Aydemi̇r, Meltem Işikgöz Taşbakan, Feri̇ha Çi̇lli̇, Alper Tünger, Sercan Ulusoy
Turkish Journal of Medical Sciences
Aim: Urinary tract infections (UTIs) caused in particular by extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains are related with high morbidity and mortality, and treatment is quite difficult. These infections generally are treated by carbapenems, and their costs are high. We aimed in this study to investigate the susceptibilities of ESBL-producing E. coli strains isolated from urine cultures to fosfomycin, ciprofloxacin, amikacin and trimethoprim-sulfamethoxazole and to determine the general resistance profile in our region of these strains isolated from UTIs. Materials and Methods: Between January 2005-December 2005, ESBL-producing E. coli strains isolated from urine samples sent from various outpatient and inpatient …
Antibioresistance Of Escherichia Coli Strains Isolated From Chicken Colibacillosis In Western Algeria, Abdelhamid Hamoudi, Hebib Aggad
Antibioresistance Of Escherichia Coli Strains Isolated From Chicken Colibacillosis In Western Algeria, Abdelhamid Hamoudi, Hebib Aggad
Turkish Journal of Veterinary & Animal Sciences
In western Algeria, 251 Escherichia coli isolates were recovered from broilers with clinical signs and lesions of colibacillosis. Serotyping showed that 82% of the isolates belong to one of the following serotypes: O78, O2, and O1. Antibiograms revealed a high level of resistance to oxytetracycline (82%), amoxicilline and ampicilline (47%), trimethoprim-sulfmethoxazole (42%), oxolinic acid and flumequine (31%). However, only a low percentage of strains were resistant to enrofloxacine (6%) and colistine (3%). Although multiresistance was common, low association was found between the serotype and antibioresistance.
Detection Of The Eaea Gene In Escherichia Coli From Chickens By Polymerase Chain Reaction, Ayşe Kiliç, Hasan Basri̇ Ertaş, Adi̇le Muz, Gökben Özbey, Hakan Kalender
Detection Of The Eaea Gene In Escherichia Coli From Chickens By Polymerase Chain Reaction, Ayşe Kiliç, Hasan Basri̇ Ertaş, Adi̇le Muz, Gökben Özbey, Hakan Kalender
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in E. coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. E. coli was isolated from 48% (48/100) of the samples. DNA was extracted from these isolates and was amplified by PCR, using a pair of primers derived from the eaeA (virulence) gene. In the agarose gel examination of PCR products, 48% (48/100) of the isolates were determined …
Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen
Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen
Turkish Journal of Veterinary & Animal Sciences
In this study, 130 Escherichia coli strains isolated from 306 cattle and sheep fecal samples were studied for their resistance to 10 antibiotics, verotoxin production, and hemolyzation. The antibiotic resistance rates of the E. coli strains were as follows: tetracycline, 51.6%; streptomycin, 24.2%; ampicillin, 13.1%; amoxicillin/clavulanic acid, 5.2%; gentamycin, 4.6%; ciprofloxacin, 4.6%; trimethoprim-sulfamethoxazole, 4.3%; cefotaxime, 0.7%. None of the strains were resistant to cefepime or ceftazidime. Of all the antibiotics tested, only resistance to streptomycin was higher in the strains isolated from cattle than in the sheep strains (P = 0.043). The evaluation of the cattle strains, based on gender …
Virulence Properties Of Escherichia Coli Isolated From Clinical Bovine Mastitis, Leyla Güler, Kadri̇ Gündüz
Virulence Properties Of Escherichia Coli Isolated From Clinical Bovine Mastitis, Leyla Güler, Kadri̇ Gündüz
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to detect the presence of the genes for some virulence factors in Escherichia coli isolates from clinical bovine mastitis. A total of 100 E. coli isolates, each isolated from individual cows in different herds between 2000 and 2005, were examined. Two multiplex polymerase chain reaction (PCR) assays were used to detect the presence of the genes encoding Shiga toxins 1 and 2 (stx1 and stx2), intimin (eaeA), heat-stable enterotoxin a (Sta), and F5 (K99), F41, and F17 fimbriae. In the PCR assays, 9 isolates were positive for the genes of F17 fimbriae and only …
Effect Of Enterococcus Faecium On The Digestive Tract Of Poultry As A Probiotic, Miroslava Kacaniova, Vladimir Kmet, Juraj Cubon
Effect Of Enterococcus Faecium On The Digestive Tract Of Poultry As A Probiotic, Miroslava Kacaniova, Vladimir Kmet, Juraj Cubon
Turkish Journal of Veterinary & Animal Sciences
This project investigates the microbiological and biochemical characteristics of chicken and turkey caeca. Microbiological characteristics were represented by CFU of Escherichia coli, faecal Enterococci and Lactobacilli determined in 1 g of chyme and biological ones by enzymatic activity of the cellulases expressed in CMC units. In all experiments, a probiotic preparation based on Enterococcus faecium was added to the feeding mixture and drinking water at various concentrations or in the form of aerosol on the body or egg surface. Counts of Escherichia coli in CFU in 1 g of faecal chyme were determined on Endo agar, counts of CFU of …
Dna Hybridization Of Escherichia Coli Strains Isolated From Uteri And Fecal Samples Of Bitches With Pyometra, Azi̇z Arda Sancak, Gurmeet Kaur Dhaliwal
Dna Hybridization Of Escherichia Coli Strains Isolated From Uteri And Fecal Samples Of Bitches With Pyometra, Azi̇z Arda Sancak, Gurmeet Kaur Dhaliwal
Turkish Journal of Veterinary & Animal Sciences
Escherichia coli is the most common bacterium that has been isolated from the bacterial culture of uterine and fecal samples of dogs with pyometra. The aim of the present study was to determine whether this organism could be relevant to the pathogenesis of pyometra in dogs. Fecal and uterine samples were collected from 17 bitches with pyometra. E. coli strains were isolated in all samples. Representative colonies of E. coli from each sample were analyzed for pathogenicity determinants by hybridization with HRP-conjugated DNA probes for enteropathogenic (eaeA), verotoxigenic (VT1, VT2), enterohemorrhagic (EHEC), enterotoxigenic (ETEC), enteroaggregative (EAggEC) and enteroinvasive (EIEC) E. …
Cloning And Expression Of The Gene Encoding Industrial \Alpha-Amylase From Bacillus Subtilis Orba97 In Escherichia Coli And Bacillus Subtilis Yb886, Numan Özcan
Turkish Journal of Veterinary & Animal Sciences
The \alpha-amylase encoding gene from Bacillus subtilis ORBA97 strain was cloned and expressed in Escherichia coli with the plasmid vector called pUC18 as well as in the \alpha-amylase negative Bacillus subtilis YB886 strain by using the pUB110 vector. The specific activity of the enzyme was determined to be 5.052 µmol (mg protein min)^{-1}. Zymogram analysis revealed no sign of protease degradation in the YB886 strain. The optimum temperature and pH of the enzyme were found to be 50°C and 5 respectively. Relative enzyme activity decreased to 36% of the original activity when it was subjected to a temperature of 90°C …
Cloning And Expression Of Bacteriophage T4 Lysozyme Gene (Gene E) In Escherichia Coli Via Pcr Amplification, Ali̇ İrfan Güzel, Numan Özcan, Hali̇l Kasap
Cloning And Expression Of Bacteriophage T4 Lysozyme Gene (Gene E) In Escherichia Coli Via Pcr Amplification, Ali̇ İrfan Güzel, Numan Özcan, Hali̇l Kasap
Turkish Journal of Veterinary & Animal Sciences
Lysozyme (EC 3.2.1.17) is an enzyme that catalyzes the hydrolysis of \beta(1-->4) glycosidic bonds between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), which are present in peptidoglycan heteropolymers of the prokaryotic cell wall. The purpose of this study was the cloning and expression of the bacteriophage T4 lysozyme gene (gene e) via PCR amplification in Escherichia coli using the pUC18 and pRS416 vectors. PCR amplification is carried out by using the primers having Bam HI recognition sites at their 5' ends. After digesting the vectors and PCR product with Bam HI endonuclease, two recombinant plasmids were constructed by ligation reaction …
Colicin Production And Colicin Typing Of Uropathogenic Escherichia Coli, Ayli̇n İmren Çarikçi, Güner Coşar
Colicin Production And Colicin Typing Of Uropathogenic Escherichia Coli, Ayli̇n İmren Çarikçi, Güner Coşar
Turkish Journal of Medical Sciences
Colicins are bactericidal macromolecules in protein structures and they are produced by some strains of Escherichia coli and Shigella, which are members of the Enterobacteriaceae. They have a bactericidal effect on the same species or closely related bacteria, but have no effect on the strains which produce them. In this study, 129 E. coli strains isolated from urine samples of patients in Izmir and Manisa were examined for colicin production, and the colicinogenic strains were examined for their colicin types by means of Horak specific indicator strains. Indicator strains were obtained from the National Type Culture Collection Hygiene and Epidemiology …