Medicine and Health Sciences Commons^™

Immunological Compatibility Status Of Placenta-Derived Stem Cells Is Mediated By Scaffold 3d Structure, Sara Azizian, Fatemeh Katami, Khashayar Modaresifar, Nariman Mosaffa, Habibollah Peirovi, Lobat Tayebi, Soheyl Bahrami, Heinz Redl, Hassan Niknejad

School of Dentistry Faculty Research and Publications

Placenta-derived amniotic epithelial cells (AECs), a great cell source for tissue engineering and stem cell therapy, are immunologically inert in their native state; however, immunological changes in these cells after culture and differentiation have challenged their applications. The aim of this study was to investigate the effect of 2D and 3D scaffolds on human lymphocyte antigens (HLA) expression by AECs. The effect of different preparation parameters including pre-freezing time and temperature was evaluated on 3D chitosan–gelatine scaffolds properties. Evaluation of MHC class I, HLA-DR and HLA-G expression in AECs after 7 d culture on 2D bed and 3D scaffold of …