Medicine and Health Sciences Commons^™

Distinct Nrf2 Signaling Thresholds Mediate Lung Tumor Initiation And Progression, Janine M. Deblasi

USF Tampa Graduate Theses and Dissertations

NRF2 is a redox-responsive transcription factor the directs the antioxidant program and several critical metabolic processes. Mutations in NRF2 or its negative regulator KEAP1 occur in up to one third of non-small cell lung cancers (NSCLCs) and are often associated with resistance to therapy and poor outcomes. In the present studies, murine alleles of the Keap1 and Nrf2 mutations found in human NSCLC were developed and I comprehensively investigated their impact on tumor initiation and progression. I observed that chronic Nrf2 stabilization by Keap1 loss-of-function or Nrf2 activating mutation was not sufficient to cause lung tumor initiation, even when p53 …

The Role Of Recreation In Preventing Delinquency And Misdemeanor In Children, Fidel Toker, Hatice Guclu Nergiz

University of South Florida (USF) M3 Publishing

The active role of recreation should be considered in the solution of social problems. In the elimination of the psychological and environmental causes of juvenile delinquency, the prevention of crime before it is committed must be taken as the basis rather than post-criminal rehabilitation (with recreational rehabilitation). For this purpose, researches showing that the tendency of crime and violence decrease with recreational activities were compiled by document analysis method. A wide literature search was made with appropriate keywords and these studies were interpreted with published statistical data. As a result of the research, it was found that the relationship between …

Restrictions Are Lifted: The Effect Of Risk Perception Of Covid-19 On Future Travel Intentions Of Dutch Travellers, Rami K. Isaac

Journal of Global Hospitality and Tourism

This study aims to understand better the psychological impact of COVID-19, the risk perception, travel and destination risks of Dutch travellers and their intention to travel when travel restrictions due to COVID-19 are lifted. This study takes a step toward closing the gap in the literature as it provides new insights into the risk perceptions regarding COVID-19 and the travel intentions of Dutch travellers now that travel restrictions are lifted. This study employed a quantitative approach to market research. Questionnaire research was applied. The questionnaire is created with the web-based survey tool Qualtrics. 343 respondents filled in the questionnaire. The …

Examining Treatment Differences In Anxiety Disorders And Body-Focused Repetitive And Related Disorders In A Pediatric Clinic Sample, Katrina Scarimbolo

USF Tampa Graduate Theses and Dissertations

Anxiety disorders are one of the most common mental health concerns that affect youth. It is estimated about 9.4% of youth have an anxiety disorder such as panic disorder, generalized anxiety disorder, social anxiety disorder, separation anxiety disorder or specific phobias (CDC, 2022). The present study includes obsessive compulsive disorder and post-traumatic stress disorder as a part of the definition of anxiety disorders. Anxiety disorders can be comorbid with many mental health conditions. The focus of this study was on the comorbidity between anxiety disorders and body-focused repetitive behaviors (BFRBs) and related disorders. BFRBs are behaviors that involve repeated picking, …

Vascular And Auditory Changes After Repetitive Traumatic Brain Injury, Courtney Lynn Penn

USF Tampa Graduate Theses and Dissertations

Traumatic brain injury (TBI) affects over 2.87 million people in the US each year. The large majority of these injuries can be classified as mild. However, evidence suggests that even mild TBIs can lead to long-lasting symptoms that greatly impact quality of life. This is particularly true when an individual sustains multiple TBIs (repetitive TBI, rTBI). Much of the damage caused by rTBI is due to secondary injury mechanisms that cause the initial injury site to spread and worsen over time. No therapies currently exist which can mitigate secondary injury, and monitoring this phase of rTBI is difficult. Development of …

Understanding How Cancer Adapts To Stress And Therapy Using Evolutionary Game Theory, Mariyah Pressley

USF Tampa Graduate Theses and Dissertations

Adaptive responses in cancer promote disease initiation, progression, and resistance to treatment. Understanding the underlying evolutionary dynamics behind these adaptive responses is crucial for developing effective therapeutic strategies. Evolutionary game theory (EGT) has proven a useful tool in analyzing how different cellular behaviors and evolutionary forces shape the dynamics of tumor growth, response to therapy, and the emergence of resistance. In this dissertation, we utilize EGT to understand the adaptive responses of cancer through the lens of ecology and evolution. By employing fitness-generating models and exploring various ecological contexts, we aimed to unveil critical factors influencing aggressive cell phenotypes, treatment …

Reframing Resistance, Resilience, And Racial Equity In Maternal Health: A Mixed Methods Exploration Of Paternal Involvement And The Racial Disparity In Severe Maternal Morbidity, Marshara G. Fross

USF Tampa Graduate Theses and Dissertations

Background: The preventable, persistent racial inequity observed in maternal and infant death has lasting impacts on African American communities and families. Fathers, partners, and co-parents are important supports during the sensitive and vulnerable perinatal period. Emerging research has begun to demonstrate a concerted effort to move beyond the sphere of medicine and quality improvement, towards exploring and integrating social factors into targeted intervention strategies to address racial inequality in health outcomes. However, sparse literature concentrates on understanding the role of fathers and partners in peripartum care and maternal health outcomes. This study aimed to explore, understand, and describe the role …

Withaferin A And Immune Checkpoint Blocker Therapy For The Treatment Of Non-Small Cell Lung Cancer, Roukiah Khalil

USF Tampa Graduate Theses and Dissertations

Lung cancer is the first cause of cancer-related deaths in both men and women with an overall five-year survival rate of 28%. Although immune checkpoint blockers (ICBs) are currently FDA-approved for the treatment of non-small cell lung cancer (NSCLC), only 17-20% of patients achieve durable responses by the induction of immunologic memory. The lack of response in most patients can be attributed to the tumor-intrinsic or tumor-extrinsic immune resistance mechanisms. A biomarker of importance is the Programmed Death Ligand-1 (PD-L1), as higher PD-L1 expression is usually associated with a better response to ICBs. Although studies have attempted to combine ICBs …

A Healthcare Claims Investigation Of Parasomnia Epidemiology, Associations With Attention Deficit/Hyperactivity Disorder, And Rem Sleep Behavior Disorder Correlates, Anh Thy Ha Nguyen

USF Tampa Graduate Theses and Dissertations

Parasomnias, such as sleepwalking and nightmare disorder, are abnormal behaviors and emotions emanating from sleep or wake to sleep transitions. Parasomnias can lead to significant adverse outcomes – sleepwalking can result in injuries and hospitalizations, frequent nightmares are associated with self-harm and suicidal thoughts, and rapid-eye movement (REM) sleep behavior disorder (RBD) is linked to neurodegeneration. Despite a history of fascination with abnormal sleep behaviors in the general and medical communities, epidemiologic understanding of parasomnias remains limited, in part due to their amnestic nature and presumption that they are benign childhood problems. This work used the MarketScan® Commercial Claims and …

Assessment Of Iso Heart Rate Method To Estimate Metabolic Rate, Karl Williams

USF Tampa Graduate Theses and Dissertations

Thermal equilibrium is primarily maintained by the body’s ability to dissipate internal heat generated during muscle contraction. A number of methods have been used to quantify this thermal burden such as charts and observation of workplace activities and behavior, however these have varying levels of validity. ISO 8996 defines a more accurate method of metabolic rate estimation that uses heart rate and maximal work capacity (MWC); however this requires the use of laboratory settings and equipment that are rarely available in the field. Equations such as the ACSM metabolic equation for the total energy expenditure of walking, hereafter referred to …

“I’M Still Suffering”: Mental Health Care Among Central African Refugee Populations In The Tampa Bay Area, C. Danee Ruszczyk

USF Tampa Graduate Theses and Dissertations

An estimated 500 Central African refugee families have been resettled in the Tampa Bay Area since 2002 (RPC, 2022). The cycles of trauma that they have endured place them in vulnerable positions regarding their mental health. Struggling to exist within underfunded social programs that are rigid in their expectations and with the current system of reactive care vs preventative care, the refugees in Tampa are put in a difficult situation of navigating their own health and wellbeing in lieu of having the full support of the United States government and their community. I will discuss how these refugees experience and …

Evaluation Of Two Methods To Estimate Wet Bulb Globe Temperature From Heat Index, Stephi Pofanl

USF Tampa Graduate Theses and Dissertations

The Wet bulb globe temperature (WBGT) is a common method to evaluate environmental factors that contribute to occupational heat stress. The Heat Index (HI) is readily available as an alternative to exposure methods. They both assess the most important contributors that are air temperature and humidity. WBGT also includes radiant heat and air motion. To account for radiant heat and/or air motion, some investigators have suggested adjustments to HI. The purpose of this study is to explore two methods to estimate the usefulness of WBGT from HI.

WBGT data were collected in a variety of locations that included direct sun …

Improving The Subjective Well-Being Of Autistic Youth Utilizing A Positive Psychology Intervention, Nicolette Bauermeister

USF Tampa Graduate Theses and Dissertations

This study utilized a single-case multiple-baseline design to analyze the effects of a ten-week multi-component positive psychology intervention, the Well-Being Promotion Program, on the subjective well-being of Autistic youth. This thesis addressed a gap in the literature regarding the effectiveness of positive psychology interventions when administered to Autistic middle schoolers. Three Autistic middle schoolers participated in this single-case design study. Dynamic decision-making was used to stagger intervention implementation across the three participants. Life satisfaction and positive/negative affect data were collected via a Qualtrics survey that was administered twice per week. Through visual analysis and Baseline Corrected Tau calculations, it was …

Development Of A Ddpcr Multiplex To Measure The Immune Response To Borrelia Burgdorferi., Kailey Marie Mccain

USF Tampa Graduate Theses and Dissertations

Zoonotic diseases account for a significant proportion of emerging infectious diseases (EIDs), with their dynamics being heavily influenced by the environment. While the effects of the environment on population-level disease dynamics have been extensively studied, there is limited research investigating the impact of the environment on individual-level disease susceptibility and transmission. This study aims to fill this gap by creating a tool to examine the variation in immune response to Borrelia burgdorferi, the causative agent of Lyme Disease, in two competent host species, Peromyscus leucopus and Peromyscus maniculatus. Two triplex droplet digital polymerase chain reaction (ddPCR) assays were developed to …

An Analysis Of State Sex Education Policies To Explore The Inclusiveness Of School Based Sex Education, Amarah Scott

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to analyze state policies related to sex education proposed between 2018 and 2022 to determine the extent to which sex education needs of LGBTQ youth were being addressed. Previous research has shown that, on average, both abstinence-only and comprehensive sex education curricula do not include information that is relevant to LGBTQ youth. Much of the information included in sex education curricula such as pregnancy prevention and the benefits and disadvantages of condoms are most relevant to youth who identify as heterosexual. Not receiving relevant and accurate sex education puts LGBTQ youth at risk for …

Variability Of Air Sampling Results Using Air-O-Cell Cassettes, Christina M. Haworth

USF Tampa Graduate Theses and Dissertations

No current federal or state standards for mold exposure exist, partly due to the lack of accurate methods for assessing fungal exposure. Exposure to airborne fungal spores can result in several symptoms such as allergic rhinitis, coughing, and fatigue. Non-industrial occupational settings are common and fungal exposures may occur in these environments. The purpose of this study is to evaluate the meaningfulness of fungal spore trap results from samples collected in both a corporate office and a university classroom. Air-O-Cell cassettes with a Zefon Bio-Pump were used to collect one outdoor and three indoor samples at each location on three …

The Physical Activity Of Spanish Speleologists: Accomplishment Of Recommendations And Differences By Sociodemographic Variables, Miquel Pans, Laura Antón-González, Maite Pellicer-Chenoll

International Journal of Speleology

Outdoor adventure activities are becoming more popular due to their potential health benefits, although there is little scientific information on speleology as a physical activity (PA). The purpose of this study is to expand the scientific evidence in this field in two ways: (a) describe the PA performed by speleologists, taking into account the variables of interest such as gender, age, education, speleological level, participation in ‘Alpine’ explorations and body mass index (BMI) and (b) identify how far the World Health Organization’s (WHO) PA recommendations were accomplished (i.e.,150 min of moderate-intensity aerobic PA or 75 min of vigorous-intensity aerobic). A …

Contribution Of Contextual Factors And Neuropathology To Dementia, Monica E. Nelson

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that has extensive biological heterogeneity. It is not clear the extent to which this heterogeneity may be detected in participants without dementia, how it relates to incident AD dementia, and whether contextual factors may change how neuropathology relates to incident AD dementia. Therefore, this dissertation was completed using data from the Alzheimer’s Disease Neuroimaging Initiative (ADNI; n = 1,703) and the Czech Brain Aging Study (CBAS; n = 385) to address the following aims: to assess biological heterogeneity in participants without dementia, to relate this heterogeneity to incident AD dementia, and to …

Combining Frameworks To Improve Military Health System Quality And Cybersecurity, Dr. Maureen L. Schafer, Dr. Joseph H. Schafer

Military Cyber Affairs

Existing conceptual frameworks and commercially available technology could be considered to rapidly operationalize the use of Quality Measures (QM) within military health systems (Costantino et al. 2020). Purchased healthcare as well as digital healthcare services have paved the way for data collection from multiple information systems thus offering stakeholders actionable intelligence to both guide and measure healthcare outcomes. However, the collection of data secondary to Smart Devices, disparate information systems, cloud services, and the Internet of Medical Things (IOMT) is a complication for security experts that also affect clients, stakeholders, organizations, and businesses delivering patient care. We have combined three …

“They’Re Still Trying To Wrap Their Head Around Forever”: An Anatomy Of Hope For Spinal Cord Injury Patients, William A. Lucas

USF Tampa Graduate Theses and Dissertations

This dissertation draws on ethnographic data to investigate the nature of spinal cord injury (SCI) rehabilitation in Central Florida, using participant observation and interview data to understand how people with SCI (pwSCI) conceptualize their own disabilities after experiencing such radical alterations in their subjectivities. Using case studies and ethnographic vignettes, it argues that the extreme double binds in which pwSCI find themselves (where they are personally ordinarily disabled and socially extraordinarily novel; and where they are enabled resources to pursue “hopeful” therapy modalities while being designated as hopelessly disabled) is further polarized by the various legislative regimes of truth in …

Pamam- Cyclodextrin Conjugate Upregulates Brain-Derived Neurotropic Factor In Arpe-19 Cells, Gopika Ashokan

USF Tampa Graduate Theses and Dissertations

Background: Traumatic Brain Injury (TBI) is a major contributor to death and disability due to motorvehicle accidents, sports, physical abuse, and battlefield injuries. The primary insult to the brain leads to inflammation, vascular dysfunction, and oxidative stress in the brain as well as in the eye. This leads to loss of Retinal Ganglion Cells (RGCs) and downregulation of Brain derived Neurotopic Factor (BDNF). BDNF is a neurotrophic factor that binds to Tropomyosin Receptor Kinase B (TrkB) receptor to promote cell growth, survival, and differentiation. Current treatment strategies do not promote neuronal regeneration. Therefore, novel treatments are needed to restore vision …

Identifying Contributors To Disproportionality: The Influence Of Perception On Student Social, Emotional, And Academic Behavior Ratings, Chelsea Salvatore

USF Tampa Graduate Theses and Dissertations

Successful implementation of the MTSS framework involves equitable assessment and decision-making. This calls for researchers to investigate the processes utilized to identify students for early behavioral support, such as multi-informant universal social-emotional behavioral screening. The current study aimed to investigate this by first examining the usability of the Social, Academic, and Emotional Behavior Subscale (SAEBRS) and the student self-report version (mySAEBRS) across Black and White students in grades seven through nine. After examining these tools for measurement invariance, the trifactor model was employed with race as a predictor variable to examine the mean difference of Black and White students’ scores …

Intimate Conversations: A Mixed-Methods Study Of African American Father-Adolescent Sexual Risk Communication, Shanda A. Vereen

USF Tampa Graduate Theses and Dissertations

Background: Approximately 50% of African American (AA) high school students are sexually active and a large proportion of sexually active AA students report engaging in sexual risk behaviors such as not using condoms at last intercourse. Consequently, AA adolescents in the United States experience outcomes of risky sexual behaviors at high rates. Evidence shows parents have an effect on shaping adolescent attitudes, values, and beliefs regarding sex through sexual risk communication (SRC). Although parent-adolescent SRC is associated with reductions in sexual risk behaviors among AA adolescents, research has primarily focused on mothers. While parents acknowledge that SRC is traditionally viewed …

Relationships Between Leading And Trailing Indicators At Construction Sites In Yanbu Industrial City, Saudi Arabia, Anas H. Halloul

USF Tampa Graduate Theses and Dissertations

Statement of Purpose: This study sought to assess the relationship between leading and lagging indicators in construction safety Management in the Kingdom of Saudi Arabia. In particular, it sought to determine whether construction sites with higher leading indicators predicted fewer incidents/accidents.Rationale for Study: Construction safety management is a critical occupational health concern globally. Scholars have researched approaches to improve safety measures and performance in construction sites. However, there is little discourse assessing the relationship between the leading indicators and the occurrence of incidents and accidents in construction sites. General Methods: A quantitative analysis was used in this study. Data were …

How Some Arboreal Salamanders (Genus Aneides) Jump, Glide, And Generate Lift, Christian E. Brown

USF Tampa Graduate Theses and Dissertations

The jumping and subsequent aerial behaviors of arboreal lungless salamanders (genus Aneides) had never been investigated or described. The aims of this research were to (1) compare jumping performance within the family Plethodontidae with a special focus on arboreal species from genus Aneides, (2) describe the biomechanics and kinematics of aerial behaviors after a jump or extended fall, and (3) model airflow over the morphology of the most aerially adept salamanders to investigate if they are capable of generating lift. First, I recorded salamanders jumping, locomoting vertically, dropping, and flying in a vertical wind tunnel. Then, I laser-scanned and reconstructed …

Bioinformatic Analysis Predicts Microglial Dysfunction In Murine Aging, Amadu Idrisa Jalloh

USF Tampa Graduate Theses and Dissertations

Age-related disease is a growing concern as the global geriatric population increases. Neurodegenerative diseases scale unfavorably in prevalence with aging and inflict disastrous consequences to human health and well-being. These disorders are challenging to investigate because they arise from complex molecular origins. The neuroimmune system is a common factor among these diseases and microglia play an important role in maintaining homeostasis in the central nervous system. Aging progressively impairs microglia by decreasing their ability to adapt and respond to noxious environmental stimuli or injury. Microglial dysfunction aggravates neurodegenerative pathology when microglia are unable to regulate neuroinflammation effectively. We investigated aging …

Diet And Salivary Microbiome On Cardiovascular Risk And Glycemic Control In Participants With And Without Type 1 Diabetes: The Cacti Study, Tiantian Pang

USF Tampa Graduate Theses and Dissertations

Introduction: The prevalence of type 1 diabetes mellitus (T1DM) has been increasing drastically in the United States. People with T1DM are at a higher risk of developing diabetes-related complications, including cardiovascular disease (CVD). Diet is one of the most modifiable factors and healthy eating could reduce CVD risk among individuals with T1DM. The current 2015-2020 Dietary Guidelines for Americans focus on overall healthy eating patterns rather than individual foods and nutrients. A healthy dietary pattern or high-quality diet can be measured by diet quality indexes, and generally contains minimal ultra-processed foods (UPF). Although substantial evidence has linked poor diet quality …

Role Of Hla-Drb1 Fucosylation In Anti-Melanoma Immunity, Daniel K. Lester

USF Tampa Graduate Theses and Dissertations

Melanoma is the one of the most lethal skin malignancies due to its ability to rapidly metastasize and evade the immune system. One factor that influences melanoma’s ability to metastasize and evade the immune system is the tumor microenvironment. The tumor microenvironment is a complex ecosystem that consists of melanoma cells interacting with different proteins and cell types such as cytokines, extra cellular matrix proteins, and various immune cells. While different immune cells can have various implications in the tumor microenvironment, some tumor infiltrating lymphocytes (TIL) have the potential to suppress these tumors. Recent therapeutic strategies aim to enhance TILs …

Histone Deacetylase 8 Is A Novel Therapeutic Target For Mantle Cell Lymphoma And Preserves Natural Killer Cell Cytotoxic Function, January M. Watters

USF Tampa Graduate Theses and Dissertations

This study demonstrates for the first time that HDAC8 function is critical for MCL survival, and abrogating its activity in human primary NK cells does not interfere with NK IgG antibody directed ADCC therapies ex vivo. Human NK cells, isolated from healthy donors, are highly resistant to HDAC8 inhibitor treatment with PCI-34051. Even at the highest concentration, 20uM, no toxicity was observed. Conversely, MCL cell lines representative of the aggressive MCL subtype, classical MCL, were especially sensitive to PCI-34051, an HDAC8 selective inhibitor, treatment. Blocking HDAC8 activity and/or abrogating expression through shRNA silencing induced significant DNA damage, hyperacetylation of SMC3, …

Natural Disasters And Human Capital: Empirical Evidence From Indonesia, Lei Lv

USF Tampa Graduate Theses and Dissertations

In developing countries, natural disasters could destroy physical capital and adversely affect human capital accumulation by disrupting individual decisions. Such decisions play a critical role in determining individuals' human capital accumulation process and have a lifelong effect on their happiness and economic prosperity. To better understand how natural disasters affect human capital in developing countries, this dissertation uses the earthquakes in Indonesia as a natural experiment to study how this earthquake affects health, child marriage, and education. For the first chapter, I study how the 2006 Yogyakarta Earthquake a affects water-related acute disease symptomsin the short and long run. By …