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Nonallelism Of Erd And Prcd And Exclusion Of The Canine Rds/Peripherin Gene As A Candidate For Both Retinal Degeneration Loci., K Ray, Gregory M. Acland, Gustavo D. Aguirre
Nonallelism Of Erd And Prcd And Exclusion Of The Canine Rds/Peripherin Gene As A Candidate For Both Retinal Degeneration Loci., K Ray, Gregory M. Acland, Gustavo D. Aguirre
Gustavo D. Aguirre, VMD, PhD
Purpose: To determine whether early retinal degeneration (erd) and progressive rod cone degeneration (prcd), two canine hereditary retinal degenerations, are caused by allelic mutations; to determine the cDNA sequence of the canine RDS/peripherin homolog (CFRDSP); and to test whether mutations(s) in CFRDSP cause(s) either erd or prcd.
Methods: Three erd-affected dogs were crossbred to three prcd-affected dogs, and their progeny were tested by electroretinography and retinal morphology for evidence of retinal degeneration. Canine RDS/peripherin cDNA was cloned and sequenced after reverse-transcription-polymerase chain reaction (RT-PCR) of total retinal RNA. A set of overlapping fragments of CFRDSP cDNA amplified from normal and …