Medicine and Health Sciences | Open Access Articles

Development Of Spermatogonial Stem Cell Niche And Immunoexpression Of Vimentin Filaments In The Testes Of Prenatal And Postnatal Indian Buffalo (Bubalus Bubalis), Ashritha Quanisha Carlo, Devendra Pathak, Opinder Singh, Neelam Bansal

Turkish Journal of Veterinary & Animal Sciences

In this study, we established the components of the buffalo spermatogonial stem cell (SSC) niche in prenatal (38.5-280 days of gestation), neonatal, prepubertal, and adult testes histochemically and immunohistochemically. Immunohistochemically, the changes in vimentin expression were evaluated. During prenatal life, the niche of primordial germ cells was formed by the surrounding mesenchymal cells and fibroblasts. The basement membrane (BM) was not organized at this stage. At 7.6 cm crown-rump length, testicular cords with a thin BM were seen. At the late gestational period, testicular cords with well-organized BM were seen. At the neonatal stage, the testicular cords were lined with …

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Phenotypic Characterization And Differentiation Of Mesenchymal Stem Cells Originating From Adipose Tissue, Ece Çerçi̇, Hati̇ce Erdost

Turkish Journal of Veterinary & Animal Sciences

Stem cells are known as undifferentiated cells that are capable of divisions and self-renewal over an extended period. Adipose tissue-derived mesenchymal stem cells (ADSCs) are known to differentiate into many different organ and tissue cells. The purpose of this study was to characterize adipose tissue-derived mesenchymal stem cells in detail based on the expression status of certain surface markers and differentiation stages for clinical and laboratory applications. The migrating phase and mitotic property of the cells from adipose tissue were assessed under a microscope. Cell counting was performed during the first, second, and third passages. The cells were differentiated into …

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Evidence Of Vertical And Horizontal Transmission Of Francisella Noatunensis Subsp. Orientalis In Hybrid Red Tilapia (Orerochromis Sp.), Vuong Viet Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Francisellosis caused by Franciscella orientalis (formerly named: Franciscella noatunensis subspecies orientalis) has been reported as one of the most important emergent diseases of warm water fish. However, little is known about its transmission, fastidious nature, survival, and persistence in multiple environments and global presence. This study aims to investigate vertical transmission, horizontal transmission by vectors and effect of other organisms on the outbreak of francisellosis in hybrid red tilapia. To prove the transmission of F. orientalis from subclinically infected tilapia mouthbrooders to their offspring through the current practice of fry production in tilapia hatcheries, experimentally infected hybrid red tilapia broodstock …

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Correlation Of Allergen Specific Immunoglobulin To House Dust Mites And Commensal Microbes In Atopic Dogs And Identification Of Major Allergen In Dermatophagoides Farinae, Nathrada Khantavee

Chulalongkorn University Theses and Dissertations (Chula ETD)

To confirm a causative allergen in atopic dogs could test through intradermal skin testing (IDT) or allergen-specific IgE serology testing (ASIS). ASIS is more practical, convenient and safer. However, the results of ASIS could be affected by source and type of allergen. Crude allergen extracts were still used in commercial testing and protein antigens of skin microbes were not included in the panel of testing. In this study, our group developed serology test for confirming house dust mite allergy called Dermatophagoides farinae and D. pteronyssinus, and measuring specific immunoglobulin to skin microbes on dogs like Malssezia pachydermatis yeast and Staphylococcus …

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Investigating The Role Of Coyotes, Canis Latrans, In The Spread Of Parasites And Arthropod-Borne Diseases In Georgia, Usa., Ansleigh Banks

Electronic Theses and Dissertations

In order to analyze the role of coyotes, Canis latrans, as hosts of parasites and arthropod-borne diseases in the state of Georgia, USA, 38 coyotes representing 18 counties and multiple geographic regions of the state were dissected and analyzed for the presence of Dirofilaria immitis, intestinal parasites, ectoparasites, and tick-borne illnesses. Mosquitoes from the locations which the coyotes originated were trapped and analyzed for the presence of D. immitis larvae. In compliance with IACUC guidelines, coyotes were obtained from individuals who had previously hunted or trapped the coyotes for leisure. Parasites were identified based on morphological features with the use …

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Evaluations Of Health In Sympatric Free-Ranging Saguinus Imperator And Leontocebus Weddelli Using Neopterin, Urinalysis, And Blood Chemistry Analyses, Alexandra J. Sacco

All Master's Theses

This thesis is composed of one journal-ready article and one chapter that includes a preliminary interpretation of data for later publication. In summary, this thesis proposes the use of three different methods of evaluating the health status of New World Monkeys: urinary neopterin measurement, blood chemistry analyses, and urinalysis. Chapter II addresses the question of whether or not the neopterin molecule, a biomarker for cell-mediated immune system activation, is conserved across primate taxa. To do so, we collected urine samples across three New World Monkey (NWM) families including captive Leontopithecus rosalia and Pithecia pithecia, and wild populations of Leontocebus …

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Rumen Microbial Culture Library And In Vitro Analysis Of Selected Bacterial Species On Colonization Resistance Against Bovine Enteric Pathogens, Maria Antony

Electronic Theses and Dissertations

The anaerobic habitat of the four chambers in ruminant’s foregut plays a vital role in the fermentation of the food by the active involvement of the residing microbial community. The cultivability and the potential role of rumen bacteria in the field of livestock production, host health and immunity are important to study. The developed anaerobic culturing techniques enable us to culture and screen different rumen bacteria to study the anti-pathogenic effects. Whole genome sequencing and analysis help us to understand the genome level characteristics of the rumen bacterial species individually. Since the majority of the rumen biosphere is still not …

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Bovine Cellular And Innate Immune Response To Hemostatic Polysaccharides, Brian Kobylkevich

Electronic Theses and Dissertations

Chronic wounds are longstanding dermal wounds that do not heal in a normal amount of time and can commonly last for over a year. These wounds plague humans and farm animals alike. Wounds are characterized by chronic inflammation and are often painful, difficult to heal, have a high recurrence rate, and are potentially debilitating. Long term wounds that do not fully heal are distressing for both humans and animals, but understandably more resources have been directed towards humans. Some treatment options for humans may be suitable for adaptation into the field of animal care. Chronic lesions such as bovine digital …

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Senecavirus A: Pathogenicity And Interactions With Host Cell Death Pathway, Maureen Hoch Vieira Fernandes

Electronic Theses and Dissertations

Senecavirus A (SVA) is a positive sense single-strand RNA virus of the genus Senecavirus, family Picornaviridae. Senecavirus A is an emerging picornavirus of swine, which causes vesicular disease (VD) in pigs that is clinically indistinguishable from footand- mouth disease and other high consequence VDs of swine. There is evidence that SVA has been circulating in the US swine population since the late eighty’s, however, recently the virus emerged worldwide, and it has been associated with several VD outbreaks in pigs in many of the major swine producing countries. Despite the recent surge in the number of cases of …

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Seasonal Patterns In Risk Factors For Taenia Solium Transmission: A Gps Tracking Study Of Pigs And Open Human Defecation In Northern Peru, Ian W. Pray, Claudio Muro, Ricardo Gamboa, Percy Vilchez, Wayne Wakeland, William Pan, William E. Lambert, Multiple Additional Authors

OHSU-PSU School of Public Health Faculty Publications and Presentations

Background: Taenia solium (cysticercosis) is a parasitic cestode that is endemic in rural populations where open defecation is common and free-roaming pigs have access to human feces. The purpose of this study was to examine the roaming patterns of free-range pigs, and identify areas where T. solium transmission could occur via contact with human feces. We did this by using GPS trackers to log the movement of 108 pigs in three villages of northern Peru. Pigs were tracked for approximately six days each and tracking was repeated in the rainy and dry seasons. Maps of pig ranges were analyzed for …

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Three-Dimensional Echocardiographic Analysis Of The Equine Aortic Valve, Amber Mcelhinney

Honors Theses and Capstones

This research focused on 3D echocardiography of the aortic valve (AV) in horses. Aortic regurgitation (AR) can be a common finding in older horses, and in severe cases can result in decreased performance and even sudden cardiac death. Standard AR diagnosis includes 2D echocardiography from the right side of the horse. Three-dimensional echocardiography (3DE) records a pyramid of tissue, showing structures in the heart difficult to visualize in 2D methods, and can be more accurate as it does not rely as heavily on placement of the transducer. Nine images of normal AV’s were assessed, and still frames of anatomical structures …

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Effects Of Medium Chain Fatty Acid Application In Swine Feed On Porcine Epidemic Diarrhea Virus, A. B. Lerner, R. A. Cochrane, J. T. Gebhardt, S. S. Dritz, C. K. Jones, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, E. Porter, J. Anderson, P. C. Gauger, D. R. Magstadt, J. Zhang, B. Bass, T. P. Karnezos, B. De Rodas, J. C. Woodworth

Kansas Agricultural Experiment Station Research Reports

Medium chain fatty acid (MCFA) application has been identified as a promising strategy to decrease viral pathogen transmission in swine feed. Four experiments were conducted to: 1) determine if MCFAs are effective when applied to feed both prior to and after porcine epidemic diarrhea virus (PEDV) inoculation measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), 2) evaluate the effects of varying amounts and combinations of MCFA measured by qRT-PCR, and 3) evaluate selected MCFA treatments in a bioassay. In Exp. 1, treatments were arranged in a 2 × 2 + 1 factorial with the main effects of chemical treatment …

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Full-Text Articles in Medicine and Health Sciences

Development Of Spermatogonial Stem Cell Niche And Immunoexpression Of Vimentin Filaments In The Testes Of Prenatal And Postnatal Indian Buffalo (Bubalus Bubalis), Ashritha Quanisha Carlo, Devendra Pathak, Opinder Singh, Neelam Bansal

Turkish Journal of Veterinary & Animal Sciences

Phenotypic Characterization And Differentiation Of Mesenchymal Stem Cells Originating From Adipose Tissue, Ece Çerçi̇, Hati̇ce Erdost

Turkish Journal of Veterinary & Animal Sciences

Evidence Of Vertical And Horizontal Transmission Of Francisella Noatunensis Subsp. Orientalis In Hybrid Red Tilapia (Orerochromis Sp.), Vuong Viet Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Correlation Of Allergen Specific Immunoglobulin To House Dust Mites And Commensal Microbes In Atopic Dogs And Identification Of Major Allergen In Dermatophagoides Farinae, Nathrada Khantavee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Investigating The Role Of Coyotes, Canis Latrans, In The Spread Of Parasites And Arthropod-Borne Diseases In Georgia, Usa., Ansleigh Banks

Electronic Theses and Dissertations

Evaluations Of Health In Sympatric Free-Ranging Saguinus Imperator And Leontocebus Weddelli Using Neopterin, Urinalysis, And Blood Chemistry Analyses, Alexandra J. Sacco

All Master's Theses

Rumen Microbial Culture Library And In Vitro Analysis Of Selected Bacterial Species On Colonization Resistance Against Bovine Enteric Pathogens, Maria Antony

Electronic Theses and Dissertations

Bovine Cellular And Innate Immune Response To Hemostatic Polysaccharides, Brian Kobylkevich

Electronic Theses and Dissertations

Senecavirus A: Pathogenicity And Interactions With Host Cell Death Pathway, Maureen Hoch Vieira Fernandes

Electronic Theses and Dissertations

Seasonal Patterns In Risk Factors For Taenia Solium Transmission: A Gps Tracking Study Of Pigs And Open Human Defecation In Northern Peru, Ian W. Pray, Claudio Muro, Ricardo Gamboa, Percy Vilchez, Wayne Wakeland, William Pan, William E. Lambert, Multiple Additional Authors

OHSU-PSU School of Public Health Faculty Publications and Presentations

Three-Dimensional Echocardiographic Analysis Of The Equine Aortic Valve, Amber Mcelhinney

Honors Theses and Capstones

Kansas Agricultural Experiment Station Research Reports