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Chimeric Protein A/G Conjugate For Detection Of Anti–Toxoplasma Gondii Immunoglobulin G In Multiple Animal Species, John J. Schaefer, Holly A. White, Stephanie L. Schaaf, Hussni O. Mohammed, Susan E. Wade
Chimeric Protein A/G Conjugate For Detection Of Anti–Toxoplasma Gondii Immunoglobulin G In Multiple Animal Species, John J. Schaefer, Holly A. White, Stephanie L. Schaaf, Hussni O. Mohammed, Susan E. Wade
John J Schaefer
Serological testing for toxoplasmosis diagnosis remains the method of choice in human medicine due to the accessibility of the requisite sample, the difficulty in predicting the parasite’s location in the host for direct detection, and the availability of established commercial methods. In veterinary medicine, although the first 2 conditions are unchanged, there is a need for commercially produced test methods that are validated for Toxoplasma gondii detection across the range of animal species that can serve as intermediate hosts. The development of such a serological method for animals would allow the diagnosis of toxoplasmosis in individual animals and a higher …
Modification Of A Commercial Toxoplasma Gondii Immunoglobulin G Enzyme-Linked Immunosorbent Assay For Use In Multiple Animal Species, John J. Schaefer, Holly A. White, Stephanie L. Schaaf, Hussni O. Mohammed, Susan E. Wade
Modification Of A Commercial Toxoplasma Gondii Immunoglobulin G Enzyme-Linked Immunosorbent Assay For Use In Multiple Animal Species, John J. Schaefer, Holly A. White, Stephanie L. Schaaf, Hussni O. Mohammed, Susan E. Wade
John J Schaefer
A challenge faced by veterinary diagnosticians in serologic analysis for exposure to pathogens is the need for a protein conjugate capable of antibody attachment in many animal species. The advent of protein conjugates that are less specific in nature allows diagnosis across many species with little or no modification of technique. Toxoplasma gondii is an organism of veterinary interest that has been demonstrated to infect a plethora of warm-blooded animals. However, the serologic tests available for simultaneous diagnosis in this broad range are limited in number. The current study examined the use of an immunoglobulin G enzyme-linked immunosorbent assay (ELISA) …