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Full-Text Articles in Medicine and Health Sciences
Clinical Characteristics, Antimicrobial Resistance And Treatment Outcomes Of Multidrug-Resistant Escherichia Coli Infection In Dogs And Cats At A Veterinary Teaching Hospital In Thailand, Jeerawat Soonthornsit, Sukanya Apiratwarrasakul, Nathita Phumthanakorn
Clinical Characteristics, Antimicrobial Resistance And Treatment Outcomes Of Multidrug-Resistant Escherichia Coli Infection In Dogs And Cats At A Veterinary Teaching Hospital In Thailand, Jeerawat Soonthornsit, Sukanya Apiratwarrasakul, Nathita Phumthanakorn
The Thai Journal of Veterinary Medicine
This retrospective study investigated the clinical characteristics of multidrug-resistant (MDR) Escherichia coli and its antimicrobial resistance phenotypes and analyzed the treatment outcomes of MDR E. coli infection in dogs and cats. The medical data of dogs and cats diagnosed in 2020 with E. coli infection at a veterinary teaching hospital were analyzed. Of 94 cases, the frequency of MDR E. coli (66%) infection was higher than that of non-MDR E. coli (34%). MDR E. coli was significantly more frequently detected in female dogs than non-MDR E. coli (P < 0.026). The most frequent MDR E. coli isolation sites were the urinary tract in dogs and skin wounds in cats. MDR E. coli isolates from dogs were highly resistant to ampicillin (96.1%), enrofloxacin (80.4%) and tetracycline (78.4%). Resistance to ampicillin (100%), enrofloxacin (90.9%), marbofloxacin (72.7%) and tetracycline (72.7%) occurred frequently in MDR E. coli isolates from cats. Low resistance to amikacin was detected in the MDR E. coli isolates from dogs and cats. The rates of clinical cure and non-clinical cure of the MDR E. coli and non-MDR E. coli cases were not significantly different. The duration of antimicrobial treatment for MDR E. coli was significantly longer in cats (12.6 ± 5.85 days) than in non-MDR E. coli cases (7 ± 0 days) (P < 0.048). Adjunctive therapy was prescribed more frequently in MDR E. coli (20%) than non-MDR E. coli cases (8.3%). The presence of MDR E. coli was high in this study. High resistance to commonly used antimicrobial drugs and treatment complications was observed in this study.
Prevalence Of Shiga Toxin-Producing Escherichia Coli In Buffaloes On Smallholdings In Coastal Area In Bangladesh, Md Shahriar Mannan, Farzana Rabbi, Md Gholam Kibria, Sirazul Islam, Md. Bayzid, Zamila Bueaza Bupasha, Md Zohorul Islam, Md Samun Sarker, Paritosh Kumar Biswas
Prevalence Of Shiga Toxin-Producing Escherichia Coli In Buffaloes On Smallholdings In Coastal Area In Bangladesh, Md Shahriar Mannan, Farzana Rabbi, Md Gholam Kibria, Sirazul Islam, Md. Bayzid, Zamila Bueaza Bupasha, Md Zohorul Islam, Md Samun Sarker, Paritosh Kumar Biswas
The Thai Journal of Veterinary Medicine
Shiga toxin-producing Escherichia coli (STEC) is an important causal agent of food borne diseases. Although the presence of STEC has been reported in cattle, goat, and sheep in previous studies in Bangladesh, there is seemingly no information on sorbitol non-fermenting STEC (SN-F STEC) carriage in buffalo. We aimed to assess the prevalence of SN-F STEC and to identify common virulence genes in SN-F STEC strains from apparently healthy buffaloes on smallholdings in coastal area in Bangladesh. Total 308 buffaloes on smallholdings in Patenga and Anwara Upazila, Chattogram division, Bangladesh were randomly sampled by collecting a swab from the recto-anal junction …
Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen
Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
A total of 292 E. coli isolates obtained from fecal samples from pigs in Central (n = 103) and Northeastern (n = 189) provinces of Thailand were included in this study. Eighty-six E. coli isolates were phenotypically confirmed to be β- lactamases producers and screened for the presence of β-lactamase genes. The CTX-M family was most frequently identified (90.7%). The blaCTX-M-15 gene belonging to CTX-M group I (59.3%) was the most predominantly identified CTX-M genotype followed by blaCTX-M-14 (31.4%) and blaCTX-M-4 (25.6%). The blaTEM-1 gene was prevalent (75.6%). The blaCTX-M-4 and blaCTX-M-14 genes were located on conjugative plasmid. The results …
Retrospective Analysis Of The Incidence, Aetiology And Risk Factors Of Sepsis Based On Post-Mortem Examination Records In Ruminants, Batrisyia Syazana Faridon, Mohd Zamri-Saad, Annas Salleh
Retrospective Analysis Of The Incidence, Aetiology And Risk Factors Of Sepsis Based On Post-Mortem Examination Records In Ruminants, Batrisyia Syazana Faridon, Mohd Zamri-Saad, Annas Salleh
The Thai Journal of Veterinary Medicine
Sepsis is an important disease in both humans and animals. In animals, it is usually caused by septicaemia after infection and has not been well-studied. This article describes a retrospective analysis of sepsis cases in small and large ruminants submitted to the Post-Mortem Laboratory, Faculty of Veterinary Medicine, Universiti Putra Malaysia between the years 2007 and 2017. The incidence of sepsis was found to be at 40.5% in small ruminants and 38.6% in large ruminants. In both small and large ruminants, sepsis was mainly caused by bacterial infection, which was significantly (p<0.05) higher compared to parasitic and viral infections. Most cases of sepsis occur due to infections originating from the gastrointestinal tract and this is followed by the respiratory tract. Escherichia coli has been found to be the most common bacterial pathogen of sepsis in ruminants; significantly (p<0.05) higher compared to other bacterial pathogens like Klebsiella pneumoniae, Pasteurella sp., and Staphylococcus aureus. Sepsis involving E. coli has often resulted in pure isolation of the pathogen, thus being suggestive of a single infection. Underlying or concurrent infection is the most important risk factor leading to sepsis in ruminants, with significant difference (p<0.05) compared to other risk factors such as stress or malnutrition. Associations between the gastrointestinal system as the point of entry and sepsis caused by either E. coli or K. pneumoniae have been noted. This study provides an insight into a basic understanding of sepsis in ruminants.
Identification Of Genotype And Phenotype Of Antimicrobial Resistance Of Escherichia Coli Isolates From Pigs In Southern Vietnam, Do Tien Duy, Nguyen Luong Lam Anh, Nguyen Thi Kim Thoa, Phitsanuwattana K, Thongratsakul S, Carrique-Mas Jj, Le Thanh Hien, Nguyen Thi Thu Nam, Nguyen Tat Toan
Identification Of Genotype And Phenotype Of Antimicrobial Resistance Of Escherichia Coli Isolates From Pigs In Southern Vietnam, Do Tien Duy, Nguyen Luong Lam Anh, Nguyen Thi Kim Thoa, Phitsanuwattana K, Thongratsakul S, Carrique-Mas Jj, Le Thanh Hien, Nguyen Thi Thu Nam, Nguyen Tat Toan
The Thai Journal of Veterinary Medicine
Escherichia coli is a primary reservoir of antimicrobial resistance, known chiefly for the container of AMR-encoding genes (ARGs), and poses potential risks to human and animal health. This study investigated AMR phenotypes and ARGs in 90 E. coli isolates from different pig groups in 10 farms in southern Vietnam. The minimum inhibitory concentration (MIC) of 19 common antimicrobial agents was determined, and polymerase chain reaction (PCR) was used to investigate seven ARGs (blaTEM, aadA1, strA, dfrA12, sul3, cmlA and tetA). Cohen’s kappa statistic (κ) was applied to assess the concordance between phenotypic and genotypic profiles. A total of 81.1% of …
A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi
A Comparative Phylotyping Analysis Of E. Coli Originating From Avian Colibacillosis Based On The Clermont Triplex Scheme And Gyra Gene Sequencing, Mohsen Amiri, Elham Ahmadi
Turkish Journal of Veterinary & Animal Sciences
Avian colibacillosis (AC) is frequently reported from poultry farms in the west of Iran. The aim of the present survey was to evaluate the phylogeny of Escherichia coli isolated from AC through the Clermont triplex protocol and gyrA gene sequencing. One hundred and fifty fresh carcasses suspected of having died from colibacillosis were sampled. The specimens were examined for E. coli isolation and identification. The extracted DNA repertoire of the isolates was subjected to phylogenetic analysis using Clermont triplex PCR. A DNA fragment of 1.8 kbp of the gyrA gene was amplified from a representative of each phylogroup or subgroup …
Antimicrobial Resistance And Phenotypic And Molecular Detection Of Extended-Spectrum Ss-Lactamases Among Extraintestinal Escherichia Coli Isolated From Pneumonic And Septicemic Sheep And Goats In Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Jyoti Kumar, Shivendra Kumar Dixit, Rajendra Kumar Meena, Bhupendra Nath Tripathi
Antimicrobial Resistance And Phenotypic And Molecular Detection Of Extended-Spectrum Ss-Lactamases Among Extraintestinal Escherichia Coli Isolated From Pneumonic And Septicemic Sheep And Goats In Rajasthan, India, Fateh Singh, Ganesh Gangaram Sonawane, Jyoti Kumar, Shivendra Kumar Dixit, Rajendra Kumar Meena, Bhupendra Nath Tripathi
Turkish Journal of Veterinary & Animal Sciences
The present study was planned to isolate Escherichia coli from pneumonic and septicemic sheep and goats and to determine antimicrobial resistance (AMR) patterns of the isolates and evaluate them for the presence of extended-spectrum ß-lactamase (ESBL) using the phenotypic and molecular methods. Pneumonic lung tissues and heart blood samples were collected by performing necropsy on sheep (n = 96) and goats (n = 08). The samples were processed and used for bacterial isolation. E. coli isolates (n = 58) including 53 from 34 sheep and 5 from 3 goats were recovered and identified by the cultural and biochemical characteristics and …
Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey, Ni̇lgün Ünal, Alper Karagöz, Şi̇nasi̇ Aşkar, Zahi̇de Di̇li̇k, Buket Yurteri̇
Extended-Spectrum β-Lactamases Among Cloacal Escherichia Coliisolates In Healthy Broilers In Turkey, Ni̇lgün Ünal, Alper Karagöz, Şi̇nasi̇ Aşkar, Zahi̇de Di̇li̇k, Buket Yurteri̇
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broiler slaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified using a BBL Crystal Enteric/Nonfermenter ID Kit (Becton Dickinson, USA) and ESBL production was confirmed using an ESBL phenotypic confirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis (PFGE) was performed with XbaI for the clonal typing of ESBL-producing E. coli isolates. While 33 phenotypic …
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Turkish Journal of Veterinary & Animal Sciences
Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …
Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters, Alma Carrascosa, Alejandro De La Peña, Lilia Gutierrez, Hector Sumano
Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters, Alma Carrascosa, Alejandro De La Peña, Lilia Gutierrez, Hector Sumano
Turkish Journal of Veterinary & Animal Sciences
Pharmacokinetics (PK), pharmacodynamics (PD), and PK/PD ratios of a new solvate of enrofloxacin (enrofloxacin hydrochloride-dihydrate; enro-C), were studied in hamsters. Enrofloxacin from Baytril1397904493 5% (enroR) served as the reference preparation. Two groups of 60 Syrian golden hamsters were intramuscularly injected individually with 10 mg/kg of enroR or with enro-C. Tissue and serum samples were obtained for 72 h; enrofloxacin concentrations were determined by HPLC with UV detection. Ninety percent minimum inhibitory concentrations (MIC90) were determined for strains of methicillin-resistant Staphylococcus aureus, Leptospira interrogans, and Escherichia coli. All PK variables were statistically different between groups (P < 0.01). CMAX of enrofloxacin for enro-C was 17.3 μg/mL and it was 2.6 μg/mL for enroR. AUC was considerably higher for enro-C (459.2 μg/mL h vs. 19.9 μg/mL h). There were no statistically significant differences in MIC90 values between enroR and enro-C. Tissue concentrations of enro-C in all cases were higher and remained above the MIC for longer periods than those of enroR. Relevant PK/PD ratios for enrofloxacin (AUC/MIC ? 125 and CMAX > MIC = 10-12) are consequently …
Prevalence Of Salmonella Enterica, Escherichia Coli And Staphylococcus Aureus In Raw Meat In Thai Self-Service Style Restaurants In Khon Kaen Municipality, Sunpetch Angkititrakul, Arunee Polpakdee, Rungtip Chuanchuen
Prevalence Of Salmonella Enterica, Escherichia Coli And Staphylococcus Aureus In Raw Meat In Thai Self-Service Style Restaurants In Khon Kaen Municipality, Sunpetch Angkititrakul, Arunee Polpakdee, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
One hundred and fifty eight raw meat samples were collected from 33 Thai self-service style restaurants in Khon Kaen municipality during July-October 2011. The fresh meat included pork, beef and chicken and was provided for self-grilling or boiling. All the samples were examined for contamination of three major foodborne pathogens including Salmonella enterica, Escherichia coli and Staphylococcus aureus using standard protocol. High prevalence of S. enterica (44%), E. coli (60%) and S. aureus (25%) was observed. Seventy-seven percent of the samples were positive for at least one of the three pathogens, while only 8% were found to carry all three …
Horner's Syndrome Associated With Escherichia Coli Infection In A Raccoon (Procyon Lotor) - A Case Report, Fernando Nájera Muñoz, Alfredo Carlos Suárez Saavedra
Horner's Syndrome Associated With Escherichia Coli Infection In A Raccoon (Procyon Lotor) - A Case Report, Fernando Nájera Muñoz, Alfredo Carlos Suárez Saavedra
The Thai Journal of Veterinary Medicine
This clinical case describes a three-year-old male raccoon (Procyon lotor), castrated, from La Lajita Oasis ParkZoological Park (Spain) which showed lack of appetite, state of mental depression and mild dehydration. Duringclinical examination an abscess was detected in the left ventrolateral area of the neck as well as clinical signscompatible with Horner’s Syndrome in the left eye. A hypersensitivity test was carried out by sympatheticinnervation with topical epinephrine 0.0001% and results suggested the presence of second-order Horner’s Syndromeformed by pressure from the abscess on the sympathetic innervation of the left eye. A radiologic study of the neckwas performed and blood and …
Plasmid Profiles Of Multidrug-Resistant Escherichia Coli From Clinically Healthy Swine, Khin Khin Lay, Nisit Chansong, Rungtip Chuanchuen
Plasmid Profiles Of Multidrug-Resistant Escherichia Coli From Clinically Healthy Swine, Khin Khin Lay, Nisit Chansong, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
A total of 145 Escherichia coli isolates originated from clinically-healthy pigs were analyzed for plasmidprofiles. All but one isolate (n=144) carried one to nine plasmids with size ranging from 0.7-16.2 Mda. Fifty-sixplasmid profiles were defined based on sizes and numbers of plasmids in each strain, of which the most commonpatterns were 15.8, 4, 3.3, 1.7, 1.3 Mda and 16.2, 15.8, 4, 3.3, 2.3, 1.7, 1.3, 1, 0.7 Mda. The results indicate widedistribution of plasmids among the commensal E. coli isolates originated from clinically-healthy pigs.
Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis
Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis
Turkish Journal of Veterinary & Animal Sciences
A 4-year-old Chios ewe was submitted to the Clinic of Farm Animals (School of Veterinary Medicine, Aristotle University of Thessaloniki). The animal had delivered 2 healthy lambs, 2 days earlier, and was inappetent, depressed, and reluctant to move, while it had tachycardia and tachypnea. The cervix was opened at a dilation of 1 finger in diameter and fetid fetal membranes expelled from the vagina. Serum biochemistry revealed renal and liver disease, while ultrasonography revealed uterine dilatation. Laparotomy revealed a partly autolysed embryo in the uterus and gangrenous metritis. Clostridium perfringens and Escherichia coli were isolated in bacterial cultures from the …
Prevalence Of The Cellular And Molecular Antimicrobial Resistance Against E. Coli Isolated From Thai Broilers, Sarawoot Mooljuntee, Piyarat Chansiripornchai, Niwat Chansiripornchai
Prevalence Of The Cellular And Molecular Antimicrobial Resistance Against E. Coli Isolated From Thai Broilers, Sarawoot Mooljuntee, Piyarat Chansiripornchai, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
The antimicrobial resistant characteristics both of the cellular and molecular levels of Escherichia coli in broilers were performed by disk diffusion test and polymerase chain reaction technique. Specific primers for the aadA, aac(3)-IV, cmlA, cat1, tetA, sul1, SHV, CITM, ereA and dhfrV gene were used to detect resistance to gentamicin, chloramphenicol, tetracycline, cephalothin, ampicillin, erythromycin, sulfonamide+trimetroprim. The 30 samples of E. coli were found to be 100% antimicrobial disk resistant to tetracycline, ampicillin and erythromycin and revealed a 90%, 93.3% and 73.3% resistant gene to tetA, CITM and ereA, respectively. While resistance to 73.3% cephalothin and 26.7% sulfonamide+trimethoprim was revealed …
The Contribution Of Active Efflux In Reduced Susceptibilities To Copper Sulfate And Zinc Chloride In Escherichia Coli Isolates From Swine, Chailai Koowatananukul, Nisit Chansong, Rungtip Chuanchuen
The Contribution Of Active Efflux In Reduced Susceptibilities To Copper Sulfate And Zinc Chloride In Escherichia Coli Isolates From Swine, Chailai Koowatananukul, Nisit Chansong, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
A hundred-eighty Escherichia coli isolates from swine were tested for minimal inhibitory concentrations (MICs) of 9 antimicrobials and two heavy metals copper sulfate and zinc chloride. All the E. coli isolates were resistant to at least one antibiotic and 175 (97.2%) isolates were multi-drug resistant. Forty antibiotic resistance patterns were identified, of which the most common pattern was AMP-CHP-CIP-ERY-GEN-STR-SUL-TET-TRI (13.3%). All the isolates formed one large population of susceptibility to copper sulfate and zinc chloride at the concentrations of 6.4-12.8 mM and 3.2-6.4 mM, respectively. Exposure to copper sulfate and zinc chloride at sublethal concentration did not change the susceptibility. …
Influence Of Cyclosporine A And Quercetine On Mic Values Of Danofloxacin Mesylate In Escherichia Coli Strains Isolated From Poultry, Nikolina Velizarova Rusenova, Aneliya Milanova Haritova, Parvan Rusenov Parvanov
Influence Of Cyclosporine A And Quercetine On Mic Values Of Danofloxacin Mesylate In Escherichia Coli Strains Isolated From Poultry, Nikolina Velizarova Rusenova, Aneliya Milanova Haritova, Parvan Rusenov Parvanov
Turkish Journal of Veterinary & Animal Sciences
A reference strain Escherichia coli ATCC 25922, sensitive and resistant strains of E. coli, isolated from chickens with colibacillosis were tested. The aim of the present study was to determine the effect of modulators of ABC efflux transporters in eukaryotic cells cyclosporine A and quercetine on MIC values of danofloxacin mesylate. MIC values of danofloxacine mesylate against the chosen strains were determined with or without modulators at concentrations of 25 \mu M, 5 \mu M, 1 \mu M, and 0.2 \mu M by macro broth dilution method. Results show that in the presence of cyclosporine A, MIC values decreased from …
Antibioresistance Of Escherichia Coli Strains Isolated From Chicken Colibacillosis In Western Algeria, Abdelhamid Hamoudi, Hebib Aggad
Antibioresistance Of Escherichia Coli Strains Isolated From Chicken Colibacillosis In Western Algeria, Abdelhamid Hamoudi, Hebib Aggad
Turkish Journal of Veterinary & Animal Sciences
In western Algeria, 251 Escherichia coli isolates were recovered from broilers with clinical signs and lesions of colibacillosis. Serotyping showed that 82% of the isolates belong to one of the following serotypes: O78, O2, and O1. Antibiograms revealed a high level of resistance to oxytetracycline (82%), amoxicilline and ampicilline (47%), trimethoprim-sulfmethoxazole (42%), oxolinic acid and flumequine (31%). However, only a low percentage of strains were resistant to enrofloxacine (6%) and colistine (3%). Although multiresistance was common, low association was found between the serotype and antibioresistance.
Detection Of The Eaea Gene In Escherichia Coli From Chickens By Polymerase Chain Reaction, Ayşe Kiliç, Hasan Basri̇ Ertaş, Adi̇le Muz, Gökben Özbey, Hakan Kalender
Detection Of The Eaea Gene In Escherichia Coli From Chickens By Polymerase Chain Reaction, Ayşe Kiliç, Hasan Basri̇ Ertaş, Adi̇le Muz, Gökben Özbey, Hakan Kalender
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in E. coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. E. coli was isolated from 48% (48/100) of the samples. DNA was extracted from these isolates and was amplified by PCR, using a pair of primers derived from the eaeA (virulence) gene. In the agarose gel examination of PCR products, 48% (48/100) of the isolates were determined …
Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen
Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen
Turkish Journal of Veterinary & Animal Sciences
In this study, 130 Escherichia coli strains isolated from 306 cattle and sheep fecal samples were studied for their resistance to 10 antibiotics, verotoxin production, and hemolyzation. The antibiotic resistance rates of the E. coli strains were as follows: tetracycline, 51.6%; streptomycin, 24.2%; ampicillin, 13.1%; amoxicillin/clavulanic acid, 5.2%; gentamycin, 4.6%; ciprofloxacin, 4.6%; trimethoprim-sulfamethoxazole, 4.3%; cefotaxime, 0.7%. None of the strains were resistant to cefepime or ceftazidime. Of all the antibiotics tested, only resistance to streptomycin was higher in the strains isolated from cattle than in the sheep strains (P = 0.043). The evaluation of the cattle strains, based on gender …
Virulence Properties Of Escherichia Coli Isolated From Clinical Bovine Mastitis, Leyla Güler, Kadri̇ Gündüz
Virulence Properties Of Escherichia Coli Isolated From Clinical Bovine Mastitis, Leyla Güler, Kadri̇ Gündüz
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to detect the presence of the genes for some virulence factors in Escherichia coli isolates from clinical bovine mastitis. A total of 100 E. coli isolates, each isolated from individual cows in different herds between 2000 and 2005, were examined. Two multiplex polymerase chain reaction (PCR) assays were used to detect the presence of the genes encoding Shiga toxins 1 and 2 (stx1 and stx2), intimin (eaeA), heat-stable enterotoxin a (Sta), and F5 (K99), F41, and F17 fimbriae. In the PCR assays, 9 isolates were positive for the genes of F17 fimbriae and only …
Effect Of Enterococcus Faecium On The Digestive Tract Of Poultry As A Probiotic, Miroslava Kacaniova, Vladimir Kmet, Juraj Cubon
Effect Of Enterococcus Faecium On The Digestive Tract Of Poultry As A Probiotic, Miroslava Kacaniova, Vladimir Kmet, Juraj Cubon
Turkish Journal of Veterinary & Animal Sciences
This project investigates the microbiological and biochemical characteristics of chicken and turkey caeca. Microbiological characteristics were represented by CFU of Escherichia coli, faecal Enterococci and Lactobacilli determined in 1 g of chyme and biological ones by enzymatic activity of the cellulases expressed in CMC units. In all experiments, a probiotic preparation based on Enterococcus faecium was added to the feeding mixture and drinking water at various concentrations or in the form of aerosol on the body or egg surface. Counts of Escherichia coli in CFU in 1 g of faecal chyme were determined on Endo agar, counts of CFU of …
Dna Hybridization Of Escherichia Coli Strains Isolated From Uteri And Fecal Samples Of Bitches With Pyometra, Azi̇z Arda Sancak, Gurmeet Kaur Dhaliwal
Dna Hybridization Of Escherichia Coli Strains Isolated From Uteri And Fecal Samples Of Bitches With Pyometra, Azi̇z Arda Sancak, Gurmeet Kaur Dhaliwal
Turkish Journal of Veterinary & Animal Sciences
Escherichia coli is the most common bacterium that has been isolated from the bacterial culture of uterine and fecal samples of dogs with pyometra. The aim of the present study was to determine whether this organism could be relevant to the pathogenesis of pyometra in dogs. Fecal and uterine samples were collected from 17 bitches with pyometra. E. coli strains were isolated in all samples. Representative colonies of E. coli from each sample were analyzed for pathogenicity determinants by hybridization with HRP-conjugated DNA probes for enteropathogenic (eaeA), verotoxigenic (VT1, VT2), enterohemorrhagic (EHEC), enterotoxigenic (ETEC), enteroaggregative (EAggEC) and enteroinvasive (EIEC) E. …
Cloning And Expression Of The Gene Encoding Industrial \Alpha-Amylase From Bacillus Subtilis Orba97 In Escherichia Coli And Bacillus Subtilis Yb886, Numan Özcan
Turkish Journal of Veterinary & Animal Sciences
The \alpha-amylase encoding gene from Bacillus subtilis ORBA97 strain was cloned and expressed in Escherichia coli with the plasmid vector called pUC18 as well as in the \alpha-amylase negative Bacillus subtilis YB886 strain by using the pUB110 vector. The specific activity of the enzyme was determined to be 5.052 µmol (mg protein min)^{-1}. Zymogram analysis revealed no sign of protease degradation in the YB886 strain. The optimum temperature and pH of the enzyme were found to be 50°C and 5 respectively. Relative enzyme activity decreased to 36% of the original activity when it was subjected to a temperature of 90°C …
Cloning And Expression Of Bacteriophage T4 Lysozyme Gene (Gene E) In Escherichia Coli Via Pcr Amplification, Ali̇ İrfan Güzel, Numan Özcan, Hali̇l Kasap
Cloning And Expression Of Bacteriophage T4 Lysozyme Gene (Gene E) In Escherichia Coli Via Pcr Amplification, Ali̇ İrfan Güzel, Numan Özcan, Hali̇l Kasap
Turkish Journal of Veterinary & Animal Sciences
Lysozyme (EC 3.2.1.17) is an enzyme that catalyzes the hydrolysis of \beta(1-->4) glycosidic bonds between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM), which are present in peptidoglycan heteropolymers of the prokaryotic cell wall. The purpose of this study was the cloning and expression of the bacteriophage T4 lysozyme gene (gene e) via PCR amplification in Escherichia coli using the pUC18 and pRS416 vectors. PCR amplification is carried out by using the primers having Bam HI recognition sites at their 5' ends. After digesting the vectors and PCR product with Bam HI endonuclease, two recombinant plasmids were constructed by ligation reaction …