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Full-Text Articles in Medicine and Health Sciences
Anti-Capsular Antibodies Activate Killing Of Escherichia Coli O8:K87 By The Alternate Complement Pathway In Porcine Serum, N. M. Clark, E. M. Berberov, M. Wang, Rodney A. Moxley
Anti-Capsular Antibodies Activate Killing Of Escherichia Coli O8:K87 By The Alternate Complement Pathway In Porcine Serum, N. M. Clark, E. M. Berberov, M. Wang, Rodney A. Moxley
School of Veterinary and Biomedical Sciences: Faculty Publications
Enterotoxigenic Escherichia coli (ETEC) strains that produce K88 (F4) + fimbria are important causes of diarrhea and post-diarrheal septicemia in swine. ETEC O8:K87, a serotype represented by a number of these strains, is typically serum resistant. Strain-specific antibodies are known to activate alternative C pathway-mediated killing of other serum-resistant E. coli [Hill, A.W., Shears, A.L., Hibbitt, K.G., 1978. The requirement of specific antibody for the killing of E. coli by the alternate complement pathway in bovine serum. Immunology 34, 131–136], but their antigenic targets have not been determined. We tested the hypothesis that anti-K87 antibodies activate alternative pathway-mediated killing of …
Sub-Typing Of Prrsv Isolates By Means Of Measurement Of Cross-Neutralization Reactions, Fernando A. Osorio
Sub-Typing Of Prrsv Isolates By Means Of Measurement Of Cross-Neutralization Reactions, Fernando A. Osorio
School of Veterinary and Biomedical Sciences: Faculty Publications
The degree of similitude or closeness between two different isolates or strains of PRRSV is very important for deciding which strains should be used for immunization, by either vaccinating with commercial vaccines or attempting to stabilize a herd with planned wt PRRSV infection. As previously known for certain important viral diseases, such as in Foot and Mouth Disease, the reciprocal (cross-) neutralization titers between two strains may be of utmost importance to establish the degree of similarity or difference between these. There is currently a void of methods that would allow distinguishing or grouping strains of PRRSV in a manner …
Visualization Of Intracellular Transport Of Vesicular Stomatitis Virus Nucleocapsids In Living Cells, Subash C. Das, Debasis Nayak, You Zhou, Asit K. Pattnaik
Visualization Of Intracellular Transport Of Vesicular Stomatitis Virus Nucleocapsids In Living Cells, Subash C. Das, Debasis Nayak, You Zhou, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
The phosphoprotein (P) of vesicular stomatitis virus (VSV) is a subunit of the viral RNA polymerase. In previous studies, we demonstrated that insertion of 19 amino acids in the hinge region of the protein had no significant effect on P protein function. In the present study, we inserted full-length enhanced green fluorescent protein (eGFP) in frame into the hinge region of P and show that the fusion protein (PeGFP) is functional in viral genome transcription and replication, albeit with reduced activity. A recombinant vesicular stomatitis virus encoding PeGFP in place of the P protein (VSV-PeGFP), which possessed reduced growth kinetics …
Significance Of Heat-Stable And Heat-Labile Enterotoxins In Porcine Colibacillosis In An Additive Model For Pathogenicity Studies, Weiping Zhang, Emil M. Berberov, Jessica Freeling, D. He, Rodney A. Moxley, David H. Francis
Significance Of Heat-Stable And Heat-Labile Enterotoxins In Porcine Colibacillosis In An Additive Model For Pathogenicity Studies, Weiping Zhang, Emil M. Berberov, Jessica Freeling, D. He, Rodney A. Moxley, David H. Francis
School of Veterinary and Biomedical Sciences: Faculty Publications
Although heat-stable (ST) and heat-labile (LT) enterotoxins produced by enterotoxigenic Escherichia coli (ETEC) have been documented as important factors associated with diarrheal diseases, investigations assessing the contributions of individual enterotoxins to the pathogenesis of E. coli infection have been limited. To address the individual roles of enterotoxins in the diarrheal disease caused by K88-positive ETEC in young pigs, enterotoxin-positive and -negative isogenic E. coli strains were constructed by using pBR322 to clone and express LT and STb. Four strains, K88+ astA, K88+ astA/pBR322, K88+ astA STb+, and K88+ astA LT+, were constructed …
Development Of Luminescent Mycobacterium Avium Subsp. Paratuberculosis For Rapid Screening Of Vaccine Candidates In Mice, Valerie Rosseels, Virginie Roupie, Denise K. Zinniel, Raul G. Barletta, Kris Huygen
Development Of Luminescent Mycobacterium Avium Subsp. Paratuberculosis For Rapid Screening Of Vaccine Candidates In Mice, Valerie Rosseels, Virginie Roupie, Denise K. Zinniel, Raul G. Barletta, Kris Huygen
School of Veterinary and Biomedical Sciences: Faculty Publications
Mycobacterium avium subsp. paratuberculosis is a slowly growing mycobacterial species, requiring 6 to 8 weeks of culture before colonies can be counted visually. Here, we describe the development of luminescent M. avium subsp. paratuberculosis expressing luxAB genes of Vibrio harveyi and its use for vaccine testing in an experimental mouse model, replacing fastidious CFU counting by rapid luminometry.
Herpes Simplex Virus Type 1 Icp0 Localizes In The Stromal Layer Of Infected Rabbit Corneas And Resides Predominantly In The Cytoplasm And/Or Perinuclear Region Of Rabbit Keratocytes, Naoyuki Morishige, James V. Jester, Julie Naito, Nelson Osorio, Andrew Wahlert, Clinton J. Jones, Roger D. Everett, Steven L. Weschler, Guey Chuen Perng
Herpes Simplex Virus Type 1 Icp0 Localizes In The Stromal Layer Of Infected Rabbit Corneas And Resides Predominantly In The Cytoplasm And/Or Perinuclear Region Of Rabbit Keratocytes, Naoyuki Morishige, James V. Jester, Julie Naito, Nelson Osorio, Andrew Wahlert, Clinton J. Jones, Roger D. Everett, Steven L. Weschler, Guey Chuen Perng
School of Veterinary and Biomedical Sciences: Faculty Publications
Herpes stromal keratitis (HSK) results from the reactivation of herpes simplex virus type-1 (HSV-1) in the cornea. The subsequent corneal inflammation and neovascularization may lead to scarring and visual loss. The cellular and molecular mechanisms underlying HSK remain unknown. The presence of stromal HSV-1 viral proteins or antigens in the HSK cornea remains a subject of debate. It was recently reported that HSV-1 ICP0 rapidly diffuses out of infected rabbit corneas. To investigate further the presence of HSV-1 ICP0 in the infected cornea, particularly in the corneal stroma, ex vivo confocal microscopy was used to scan rabbit corneas infected with …
Bovine Herpesvirus 1 Immediate-Early Protein (Bicp0) Interacts With The Histone Acetyltransferase P300, Which Stimulates Productive Infection And Gc Promoter Activity, Yange Zhang, Yunquan Jiang, Vicki Geiser, Joe Zhou, Clinton J. Jones
Bovine Herpesvirus 1 Immediate-Early Protein (Bicp0) Interacts With The Histone Acetyltransferase P300, Which Stimulates Productive Infection And Gc Promoter Activity, Yange Zhang, Yunquan Jiang, Vicki Geiser, Joe Zhou, Clinton J. Jones
School of Veterinary and Biomedical Sciences: Faculty Publications
The immediate-early protein, bICP0, of Bovine herpesvirus 1 (BHV-1) transactivates viral promoters and stimulates productive infection. bICP0 is expressed constitutively during productive infection, as its gene contains an immediate- early and an early promoter. Like other ICP0 homologues encoded by members of the subfamily Alphaherpesvirinae, bICP0 contains a zinc RING finger located near its N terminus. Mutations that disrupt the bICP0 zinc RING finger impair its ability to activate transcription, stimulate productive infection, inhibit interferon-dependent transcription in certain cell types and regulate subnuclear localization. bICP0 also interacts with a cellular chromatin-remodeling enzyme, histone deacetylase 1 (HDAC1), and can relieve …
Effects Of Moraxella (Branhamella) Ovis Culture Filtrates On Bovine Erythrocytes, Peripheral Mononuclear Cells, And Corneal Epithelial Cells, Henry E. Cerny, Douglas G. Rogers, Jeffrey T. Gray, David R. Smith, Susanne Hinkley
Effects Of Moraxella (Branhamella) Ovis Culture Filtrates On Bovine Erythrocytes, Peripheral Mononuclear Cells, And Corneal Epithelial Cells, Henry E. Cerny, Douglas G. Rogers, Jeffrey T. Gray, David R. Smith, Susanne Hinkley
School of Veterinary and Biomedical Sciences: Faculty Publications
Infectious bovine keratoconjunctivitis (IBK) is a highly contagious ocular disease that affects cattle of all ages and that occurs worldwide. Piliated hemolytic Moraxella bovis is recognized as the etiologic agent of IBK. According to data from the Nebraska Veterinary Diagnostic Laboratory System, however, Moraxella (Branhamella) ovis has been isolated with increasing frequency from cattle affected with IBK. The objective of this study was, therefore, to examine M. ovis field isolates for the presence of the putative virulence factors of M. bovis. Culture filtrates from selected M. ovis field isolates demonstrated hemolytic activity on bovine erythrocytes and cytotoxic activity on …
Transcriptome Signature Of Virulent And Attenuated Pseudorabies Virus-Infected Rodent Brain, Christina Paulus, Patricia J. Sollars, Gary E. Pickard, Lynn W. Enquist
Transcriptome Signature Of Virulent And Attenuated Pseudorabies Virus-Infected Rodent Brain, Christina Paulus, Patricia J. Sollars, Gary E. Pickard, Lynn W. Enquist
School of Veterinary and Biomedical Sciences: Faculty Publications
Mammalian alphaherpesviruses normally establish latent infections in ganglia of the peripheral nervous system in their natural hosts. Occasionally, however, these viruses spread to the central nervous system (CNS), where they cause damaging, often fatal, infections. Attenuated alphaherpesvirus derivatives have been used extensively as neuronal circuit tracers in a variety of animal models. Their circuit-specific spread provides a unique paradigm to study the local and global CNS response to infection. Thus, we systematically analyzed the host gene expression profile after acute pseudorabies virus (PRV) infection of the CNS using Affymetrix GeneChip technology. Rats were injected intraocularly with one of three selected …