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Articles 31 - 43 of 43
Full-Text Articles in Medicine and Health Sciences
A Simple And Rapid Method For The Extraction Of Genomic Dna From Piglet Tails Without Using Proteinase K, Nathamon Yimpring, Komkrich Teankum, Prapat Suriyaphol, Nutthee Am-In, Gunnaporn Suriyaphol
A Simple And Rapid Method For The Extraction Of Genomic Dna From Piglet Tails Without Using Proteinase K, Nathamon Yimpring, Komkrich Teankum, Prapat Suriyaphol, Nutthee Am-In, Gunnaporn Suriyaphol
The Thai Journal of Veterinary Medicine
Genomic DNA (gDNA) is a prerequisite for genomic research. However, the collection of blood samples from young animals for gDNA extraction is not practical. Since piglet tail docking is routinely performed in pig farms to prevent injury from tail biting, docking tails served as a practical source for gDNA in piglets. However, extraction of gDNA from piglet tails is difficult because of inelasticity and cartilage. The purpose of this study was to develop a rapid and simple method of isolating gDNA from piglet tails for downstream molecular purpose. Chopped piglet tails with ceramic beads in lysis buffer were vortex-homogenized prior …
Molecular Detection Of Trypanosoma Evansi In Camels (Camelus Dromedarius) In Southwestern Saudi Arabia, Alarabi Mohamed Elsiddig Mohamed, Yasser Osman Mohamed, Elshafie Ibrahim Elshafie, Yasser Jabir Ali Alharbi, Hesham Mohamed Al-Mekhlafi
Molecular Detection Of Trypanosoma Evansi In Camels (Camelus Dromedarius) In Southwestern Saudi Arabia, Alarabi Mohamed Elsiddig Mohamed, Yasser Osman Mohamed, Elshafie Ibrahim Elshafie, Yasser Jabir Ali Alharbi, Hesham Mohamed Al-Mekhlafi
The Thai Journal of Veterinary Medicine
This cross-sectional study aimed to determine the prevalence of Trypanosoma evansi infection and its associated risk factors in camels in the Jazan region, southwestern Saudi Arabia. A total of 679 blood samples were collected from three local breeds of camel – Awadia, Awarkia and Sawahelle – in 11 randomly selected governorates of Jazan region. Blood samples were screened by Giemsa-stained blood film (GSBF) and polymerase chain reaction (PCR) techniques. The overall prevalence of T. evansi was 30.9% (210/679, CI: 27.6–34.5%) by PCR, whereas a prevalence of 22.2% (151/679, CI: 19.3–25.5%) was revealed by GSBF. The highest prevalence was identified in …
Identification Of Streptococcus Spp. Isolated From Bovine Milk And Characterization Of Their Antimicrobial Susceptibility Profiles In Taiwan, Jui-Chun Hsieh, Yu-Shan Yen, Shih-Te Chuang
Identification Of Streptococcus Spp. Isolated From Bovine Milk And Characterization Of Their Antimicrobial Susceptibility Profiles In Taiwan, Jui-Chun Hsieh, Yu-Shan Yen, Shih-Te Chuang
The Thai Journal of Veterinary Medicine
Bovine mastitis has had a great impact on the economic losses of the dairy industry. Nowadays, environmental pathogens cause the main types of bovine mastitis, especially the streptococci. The aim of the study was to identify the major Streptococcus species causing bovine mastitis in Taiwan as well as to reveal their antimicrobial susceptibility profiles. By colony morphology identifying, Gram staining, and subculturing on Edwards agar, the preliminary identified streptococci were subjected to biochemical identification, latex agglutination tests and dick diffusion tests for susceptibility tests of 16 antimicrobial agents. Among a total of 157 strains, Streptococcus uberis was the most dominant …
Transcriptome Analysis Of Zi Geese (Anser Cygnoides) Prehierarchical Follicles And Verification Of Related Genes In Arginine Metabolic Pathway, Yongfeng Sun, Lulu Liu, Chang Liu, Cornelius Tlotliso Sello, Huiyan Wu, Hongtao Lu, Chenguang Xu, Yujian Sui
Transcriptome Analysis Of Zi Geese (Anser Cygnoides) Prehierarchical Follicles And Verification Of Related Genes In Arginine Metabolic Pathway, Yongfeng Sun, Lulu Liu, Chang Liu, Cornelius Tlotliso Sello, Huiyan Wu, Hongtao Lu, Chenguang Xu, Yujian Sui
The Thai Journal of Veterinary Medicine
Arginine metabolism plays a pivotal role in the mammalian reproductive system by controlling various physiological processes of the reproductive organs including follicular development, ovulatory mechanisms and egg production. However little is known about the molecular mechanisms regulating arginine metabolism at early stages of folliculogenesis. The objective of the current study was to determine the arginine metabolic pathways in prehierarchical follicles of Zi geese through the Illumina Hiseq 2500 platform and their transcripts through the de novo assembly. In this study, five different stages of the geese prehierarchical follicles transcriptome were sequenced. A total of 31722729162 bp from 224929214 high quality …
Surgical Removal And Recurrence Of Leiomyosarcoma In A King Cobra, Ophiophagus Hannah, Taksa Vasaruchapong, Panithi Laoungbua, Lawan Chanhome, Wijit Banlunara
Surgical Removal And Recurrence Of Leiomyosarcoma In A King Cobra, Ophiophagus Hannah, Taksa Vasaruchapong, Panithi Laoungbua, Lawan Chanhome, Wijit Banlunara
The Thai Journal of Veterinary Medicine
An adult male wild-caught king cobra, Ophiophagus hannah, of unknown age was raised in captivity for seven years. The snake showed swelling at the caudal third of the body. Physical examination revealed an approximately 15x10 cm firm mass in coelomic cavity. Radiography showed soft tissue density causing complete obstruction of intestinal tract. The mass was surgically removed and diagnosed as leiomyosarcoma. The snake was monitored and the mass recurred two months post-surgery. A second surgical exploration showed severe adhesion of the mass to dorsal aorta, intercostal muscle, ribs and stomach. The snake was euthanized and the recurrent masses were diagnosed …
Efficacy Of Live Ndv Vaccine Simultaneously Vaccinated With Recombinant Hvt-Ndv Vaccine On Early Protection Against Newcastle Disease Virus Challenge, Nataya Charoenvisal, Jiroj Sasipreeyajan
Efficacy Of Live Ndv Vaccine Simultaneously Vaccinated With Recombinant Hvt-Ndv Vaccine On Early Protection Against Newcastle Disease Virus Challenge, Nataya Charoenvisal, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
The objective of this study was to determine the efficacy of the recombinant herpes virus of turkey-Newcastle disease virus (rHVT-NDV) vaccine against Newcastle disease virus (NDV) challenged in 14 days old broiler chickens. One hundred chickens were divided into 5 groups of 20 chickens each. Group 1 chickens received live NDV vaccine via the intra-nasal route. Group 2 chickens received rHVT-NDV vaccine by subcutaneous injection. Group 3 chickens, received both live NDV and rHVT-NDV vaccine. Groups 4 and 5 chickens did not receive any vaccine and served as positive and negative control groups. At 14 days old, all the chickens …
Effect Of Dexmedetomidine Combined With Pethidine On Minimum Alveolar Concentration Of Isoflurane In Dogs, Kanjana Vinyunantakul, Sumit Durongphongtorn, Marissak Kalpravidh
Effect Of Dexmedetomidine Combined With Pethidine On Minimum Alveolar Concentration Of Isoflurane In Dogs, Kanjana Vinyunantakul, Sumit Durongphongtorn, Marissak Kalpravidh
The Thai Journal of Veterinary Medicine
The effect of dexmedetomidine alone and when combined with pethidine on a minimum alveolar concentration (MAC) of isoflurane was determined in 24 client-owned adult male dogs before castration. Dogs were assigned to Group 1 (n = 12; dexmedetomidine, 5 µg/kg IM) or Group 2 (n = 12; dexmedetomidine, 5 µg/kgand pethidine, 5 mg/kg IM) . Sedation and response to noxious stimulation (digit clamping) were evaluated at 15 minutes after drug injection. Then, anaesthesia was induced via a face mask with isoflurane in oxygen and maintained via an endotracheal tube with an end tidal concentration of isoflurane (E´ISO%) of 1%, at …
Epidemiologic Evaluation Of Feline Urolithiasis In Thailand From 2010 To 2017, Vachira Hunprasit, Pinit Pusoonthornthum, Lori Koehler, Jody P. Lulich
Epidemiologic Evaluation Of Feline Urolithiasis In Thailand From 2010 To 2017, Vachira Hunprasit, Pinit Pusoonthornthum, Lori Koehler, Jody P. Lulich
The Thai Journal of Veterinary Medicine
This cross-sectional study describes the epidemiological data of feline urolithiasis in Thailand. Information on 923 feline uroliths submitted from Thailand to the Minnesota Urolith Center between January 2010 and December 2017 was evaluated. The frequency of urolith types, the relationships between urolith type and breeds and sex, were analyzed. Calcium oxalate was the most common urolith (49.5%) and was commonly found in male cats (OR = 1.81; 95%CI: 1.39, 2.36) with a mean age of 6.3±3.2 years. Struvite was the second most common urolith (38%), more frequently found in females (OR = 1.46; 95%CI: 1.11, 1.91) with a mean age …
Effect Of Nicotine On Gene Expression And Developmental Competence Of Bovine Oocytes, Seree Teerapong, Tassanee Faisaikarm, Natakarn Nimsanor, Chinarat Changsangfa, Kornkanok Promthep, Kulnasan Saikhun
Effect Of Nicotine On Gene Expression And Developmental Competence Of Bovine Oocytes, Seree Teerapong, Tassanee Faisaikarm, Natakarn Nimsanor, Chinarat Changsangfa, Kornkanok Promthep, Kulnasan Saikhun
The Thai Journal of Veterinary Medicine
Nicotine affected meiosis during oocyte maturation is associated with poor subsequent embryonic development. The aim of this study was to analyze the effects of nicotine exposure during in vitro maturation (IVM) on the outcomes of in vitro fertilization (IVF) and the expression of gene related development of oocytes and embryos. Immature (germinal vesicle, GV) oocytes were randomly cultured in IVM media containing nicotine at concentrations of 0.0 (control) 2.5, 5.0, and 10.0 mM. After culturing for 24 h, oocytes were assessed for their maturation rate, subjected to IVF and mRNA extraction for gene expression analysis using quantitative real time PCR …
Effect Of A Rapid Heat Treatment On Thai Qx-Like Infectious Bronchitis Virus And Evaluation Of Protection Induced By A Heat-Treated Virus, Tawatchai Pohuang, Sucheeva Junnu
Effect Of A Rapid Heat Treatment On Thai Qx-Like Infectious Bronchitis Virus And Evaluation Of Protection Induced By A Heat-Treated Virus, Tawatchai Pohuang, Sucheeva Junnu
The Thai Journal of Veterinary Medicine
The purpose of this study was to evaluate the effect of a rapid heat-treatment method on Thai QX-like infectious bronchitis virus (IBV) and the efficacy on protection from the virus after heat treatment. QX-like IBV isolate THA46 was incubated at 56 oC. The incubated virus was collected every 5 min for up to 1 h and then they were inoculated into embryonated chicken eggs. At 6 days post-inoculation, allantoic fluid was harvested from the embryonated eggs which were inoculated with virus of the longest heat-treatment time that induced lesions in the embryos. That allantoic fluid was used in a subsequent …
Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho
Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho
The Thai Journal of Veterinary Medicine
The study was performed to investigate the antimicrobial susceptibility of L. intracellularis field isolates from Korean pig farms. The novel four field isolates successfully obtained from clinical cases were prepared in IEC-18 cells to conduct the in vitro antimicrobial susceptibility testing (AST) by determining minimum inhibitory concentrations (MIC)s; either intracellular (InMIC) or extracellular MICs (ExMIC). The final MICs were assessed by counting the number of heavily infected cells (HICs;> 30 bacteria per cell) using an immunoperoxidase monolayer assay. Enrofloxacin (0.125 to 0.25 μg/mL: InMIC and 2 to 16 μg/mL; ExMIC) presented the most notable antimicrobial susceptibility, and marbofloxacin (0.25 to …
Dual Infection Of A Thai Isolate Hp-Prrsv And The Pdmh1n1 2009 Siv In Weanling Pigs, Nattaphong Sangpratum, Roongtham Kedkovid, Yonlayong Woonwong, Chaitawat Sirisereewan, Sawang Kesdangsakonwut, Roongroje Thanawongnuwech, Jirapat Arunorat
Dual Infection Of A Thai Isolate Hp-Prrsv And The Pdmh1n1 2009 Siv In Weanling Pigs, Nattaphong Sangpratum, Roongtham Kedkovid, Yonlayong Woonwong, Chaitawat Sirisereewan, Sawang Kesdangsakonwut, Roongroje Thanawongnuwech, Jirapat Arunorat
The Thai Journal of Veterinary Medicine
After the introduction of the pandemic H1N1 swine influenza virus (pdmH1N1 SIV) in Thai swine farms in 2009, a mild clinical outcome was observed and its role in porcine respiratory disease complex (PRDC) is questionable. A highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has become endemic in Thai swine farms, recently. This study was conducted to determine the pathogenicity of the pdmH1N1 SIV when co-infected with a HP-PRRSV. Thirty-two weanling pigs from a free PRRSV, SIV, porcine circovirus type 2 (PCV-2) and Mycoplasma spp. commercial farm were randomly divided into 4 groups; Negative, PRRSV, the SIV and Co-infection …
Accuracy Of Portable Human Glucose Meter (Accu-Chek® Performa) For Blood Glucose Measurement In Newborn Piglets, Morakot Nuntapaitoon, Sujin Sirisawadi, Sariya Asawakarn, Padet Tummaruk
Accuracy Of Portable Human Glucose Meter (Accu-Chek® Performa) For Blood Glucose Measurement In Newborn Piglets, Morakot Nuntapaitoon, Sujin Sirisawadi, Sariya Asawakarn, Padet Tummaruk
The Thai Journal of Veterinary Medicine
Blood glucose concentration is a significant postnatal survival indicator in newborn piglets. The standard procedure is difficult to perform under field conditions and requires a long period of time for processing. Portable glucose meters have been invented to yield a faster result and utilize a small blood sample. The present study aims to compare glucose concentration mea s ur ed using a portable human glucose meter ( Accu- chek® Performa) with the standard protocol. A total of 200 blood samples were collected from the umbilical cord of newborn piglets and were measured using the portable human glucose meter immediately after …