Medicine and Health Sciences Commons^™

Protein Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girk) In Breast Cancer Cells, Madhu S. Dhar, Howard K. Plummer

Faculty Publications and Other Works -- Large Animal Clinical Sciences

Background

Previous data from our laboratory has indicated that a functional link exists between the G-protein-coupled inwardly rectifying potassium (GIRK) channel and the beta-adrenergic receptor pathway in breast cancer cell lines, and these pathways were involved in growth regulation of these cells. Alcohol is an established risk factor for breast cancer and has been found to open GIRK. In order to further investigate GIRK channels in breast cancer and possible alteration by ethanol, we identified GIRK channel protein expression in breast cancer cells.

Results

Cell pellets were collected and membrane protein was isolated to determine GIRK protein expression. GIRK protein …

Sheep Updates 2006 - Contents, Department Of Agriculture And Food, Western Australia

Sheep Updates

No abstract provided.

Sheep Updates 2006 -Part 1, David Sackett, Kevin Foster, Ron Yates, Phil Nichols, Graeme Martin, John Milton, Scott Williams, Garry Mcalister, Mark Suttie, Peter Fennessy, Jack Cocks

Sheep Updates

This session covers seven papers from different authors: PLENARY 1. Making Dollars from Merinos, David Sackett, Holmes Sackett & Associates Pty Limited, Wagga Wagga, NSW 2. A new variety of sulla (Hedysarun coronarium)for forage production in southern Australia, Kevin Foster, Ron Yates, Phil Nichols, Department of Agriculture and Food, WA and Centre for Legumes in Mediterranean Agriculture, UWA 3. Mating - Short and fast is better, Graeme Martin, John Milton, Faculty of Natural & Agricultural Sciences, The University of Western Australia 4. Breech strike protection in sheep post 2010, Scott Williams, Program Manager Animal Health and Welfare, Australian Wool Innovation …

Sheep Updates 2006 - Part 2, Darryl Smith, Kathryn Kemper, David Rutley, R. R. Woolaston, D. J. Brown, K. D. Atkins, A. E. Casey, A. J. Ball, David Hopkins, David Stanley, Leonie Martin, Arthur Gilmour, Remy Van De Ven, Geoff Duddy, Steve Semple, David Kessell, Andrew Thompson

Sheep Updates

This session covers six papers from different authors:

GENETICS

1. Novel selection traits - what are the possible side effects?, Darryl Smith, Kathryn Kemper, South Australian Research and Development Institute, David Rutley, University of Adelaide.

2. Genetic Changes in the Australian Merino since 1900, Sheep Genetics Australia Technical Committee, R.R. Woolaston Pullenvale, Queensland, D.J. Brown, Animal Genetics and Breeding Unit*, University of New England, K.D. Atkins, A.E. Casey, NSW Department of Primary Industries, A.J. Ball, Meat and Livestock Australia, University of New England

3. Influence of Sire Growth Estimated Breeding Value (EBV0 on Progeny Growth, David Hopkins, David Stanley, Leonie …

Long Acting, Reversible Veterinary Sedative And Analgesic And Method Of Use, Thomas Tobin

Veterinary Science Faculty Patents

A veterinary composition comprising a guanidine derivative, e.g., guanabenz or guanabenz acetate is provided which produces a rapid acting and long lasting sedative and analgesic effect in a subject animal that is selectively reversible. The use of guanabenz in the horse provides for a safe, effective, long lasting and rapidly reversible sedative and analgesic which can be used on the standing animal. Methods of use of the compositions of the invention are also provided.

Disease Risks Associated With Increasing Feral Swine Numbers And Distribution In The United States, Thomas Hutton, Thomas J. Deliberto, Sheldon Owen, Bruce Morrison

Michigan Bovine Tuberculosis Bibliography and Database

Feral hogs or “wild boars” come from several sources and include released or escaped domestic swine and the truly wild European boar. When free-roaming in North America, all are included in the term “feral swine,” as are hybrids of the two types. Although morphologically distinct, both the feral swine and European wild swine are recognized as Sus scrofa. The physical damage caused by feral swine has been well documented and includes damage to vehicles, vineyards, tree plantings, archaeological sites, agricultural crops, turf, soils, rare plant communities, and wildlife habitat (Seward et al. 2004). In addition they compete with livestock …

The Ethics Of Referral, Bernard Rollin

Professional Veterinary Ethics Collection

The rapid growth of veterinary specialty practices has created a number of vexatious ethical issues relevant to veterinary medicine. The preeminent question pertains to “the duty to refer.” Do primary care practitioners have such a moral duty? If so, when does this duty arise? Does it pertain in all cases where specialized knowledge is relevant to a disease?

This raises the fundamental question of “Veterinary Ethics” — namely, does the veterinarian ideally have primary obligation to the client/owner or the animal? (1) There are 2 possible ideal types that a veterinarian can aim for — the Garage Mechanic Model or …

Visualization Of Intracellular Transport Of Vesicular Stomatitis Virus Nucleocapsids In Living Cells, Subash C. Das, Debasis Nayak, You Zhou, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein (P) of vesicular stomatitis virus (VSV) is a subunit of the viral RNA polymerase. In previous studies, we demonstrated that insertion of 19 amino acids in the hinge region of the protein had no significant effect on P protein function. In the present study, we inserted full-length enhanced green fluorescent protein (eGFP) in frame into the hinge region of P and show that the fusion protein (PeGFP) is functional in viral genome transcription and replication, albeit with reduced activity. A recombinant vesicular stomatitis virus encoding PeGFP in place of the P protein (VSV-PeGFP), which possessed reduced growth kinetics …

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message [Page] 1-Associate Director Named and Quality Moves Forward at the ADRDL Diagnostic News [Page] 1- Reminder: Changing Your Report Generator Password [Page] 1- ADRDL Quality System [Page] 2- Holiday Hours Extension News [Page] 2- Survey of Anthrax-affected Producers: Affected and Non-affected Pastures Summer 2005 [Page] 4- “Pieces and Parts” [Page] 5- Nitrates in Feedstuffs: Sampling Considerations [Page] 6- Searching for Veterinary Information: Useful Websites [Page] 7- Calendar of Events

Nucleic Acids Encoding Sarcocystis Neurona Antigen And Uses Thereof, Daniel K. Howe

Veterinary Science Faculty Patents

The present invention provides novel isolated nucleic acids encoding antigenic proteins derived from Sarcocystis neurona, or unique fragments thereof. In particular, the invention provides novel isolated nucleic acids encoding membrane-associated polypeptides SnSAG2, SnSAG3, and SnSAG4. Also provided are purified antigenic polypeptide fragments encoded by the novel nucleic acid sequences set forth herein that encode for SnSAG2, SnSAG3, and SnSAG4. Also provided are isolated nucleic acids capable of selectively hybridizing with the nucleic acid from Sarcocystis neurona. The invention also provides vectors comprising the nucleic acids of the invention encoding an antigenic protein derived from Sarcocystis neurona or …

Significance Of Heat-Stable And Heat-Labile Enterotoxins In Porcine Colibacillosis In An Additive Model For Pathogenicity Studies, Weiping Zhang, Emil M. Berberov, Jessica Freeling, D. He, Rodney A. Moxley, David H. Francis

School of Veterinary and Biomedical Sciences: Faculty Publications

Although heat-stable (ST) and heat-labile (LT) enterotoxins produced by enterotoxigenic Escherichia coli (ETEC) have been documented as important factors associated with diarrheal diseases, investigations assessing the contributions of individual enterotoxins to the pathogenesis of E. coli infection have been limited. To address the individual roles of enterotoxins in the diarrheal disease caused by K88-positive ETEC in young pigs, enterotoxin-positive and -negative isogenic E. coli strains were constructed by using pBR322 to clone and express LT and STb. Four strains, K88⁺ astA, K88⁺ astA/pBR322, K88⁺ astA STb⁺, and K88⁺ astA LT⁺, were constructed …

A Comparative Study Of Steroid Hormone Levels And Oestrogen Receptor Alpha (Erα) Between Normal Bitch Uteri And Pyometra During Dioestrus, Sayamon Srisuwatanasagul, Kongkiat Srisuwatanasagul, Sudson Sirividayapong

The Thai Journal of Veterinary Medicine

Ovarian steroid hormones and their receptors play an important role in both physiological as well as pathological changes seen in the uterus. For the further understanding of the mechanism of pyometra, in relation to steroid hormones and their receptors in the bitch, the difference between the expression of the oestrogen receptor alpha (ERa) between normal and pyometra bitches during dioestrus was investigated. Uterine samples were collected during dioestrus from normal and pyometra bitches. Immunohistochemistry was used to investigate the expression of ERa in the different compartments of the uterus. The results showed that the levels of steroid hormones in the …

Laboratory Diagnosis Of Mycoplasma Gallisepticum (Mg) Infection In Experimental Layer Chicken Receiving Mg Vaccines And Mg Organisms, Somsak Pakpinyo, Pinyo Pitayachamrat, Seubchat Saccavadit, Than Santaswang, Achara Tawatsin, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

This experiment was designed to study the results of laboratory diagnosis of Mycoplasma gallisepticum (MG) infection in layer chicken receiving MG vaccines and MG organisms. One hundred and twenty 1-day-old-malelayer chickens were raised in separate isolation rooms and equally allocated into 4 separated groups. When 4 weeks old, groups 1, 2, 3 and 4 served as a negative control, a MG commercially inactivated vaccine group, a MG commercially live vaccine group and a MG infected group, respectively. In each group, ten birds were swabbed and bled when 4, 5, 6, 7, 8, 9 and 11 weeks old. Swab samples were …

Oral Medication With Florfenicol For Black Tiger Shrimps Penaeus Monodon, Nutcharnart Tipmongkolsilp, Yanin Limpanon, Benjamas Patamalai, Pischa Lusanandana, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

Florfenicol (FF) and Chloramphenicol (CAP) were tested for their in vitro antimicrobial activity against 102 Vibrio isolates from clinical cases. The Minimum Inhibitory Concentration (MIC) for both antimicrobials was ≤ 8 μg/ml suggesting that all of the tested isolates were susceptible to both antimicrobials. The observed MIC range of FF (0.5-4.0 μg/ml) was more potent than that of CAP (0.5-8.0 μg/ml). The activity of both antimicrobials was not substantially influenced by the addition of sea water (5 ppt salinity) to the test system. When considering the best type of antimicrobial for food producing animals and in vitro antivibrio activity, FF …

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Monkon Trisiriroj

The Thai Journal of Veterinary Medicine

No abstract provided.

The Zoonotic Potential Of Enterocytozoon Bieneusi In Thailand, Mathirut Mungthin, Ittisak Subrungruang, Saovanee Leelayoova

The Thai Journal of Veterinary Medicine

Enterocytozoon bieneusi is the most common microsporidia that causes disease in immunocompromised hosts, especially in AIDS patients. E. bieneusi has been reported in a wide range of animals, including domestic, wild and farm animals. Using molecular typing and phylogenetic analysis, the zoonotic potential of E. bieneusi to cause disease is indeed a threat. Transmission from animals to humans should be investigated and whether it occurs naturally, before we can address the whole subject of zoonotic transmission of E. bieneusi infection.

Hemangiosarcoma Of The Nictitating Membrane As Seen In Two Great Danes (A Case Report), Atichat Brahmasa, Nalinee Tuntivanich, Pranee Tuntivanich, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

Hemangiosarcoma of the nictitating membrane of two Great Dane dogs is reported. Treatment was successfully carried out by amputation of the nictitan. No recurrence of the mass or pulmonary metastasis was evident within a period of twelve months after the treatment.

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Development Of Luminescent Mycobacterium Avium Subsp. Paratuberculosis For Rapid Screening Of Vaccine Candidates In Mice, Valerie Rosseels, Virginie Roupie, Denise K. Zinniel, Raul G. Barletta, Kris Huygen

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium avium subsp. paratuberculosis is a slowly growing mycobacterial species, requiring 6 to 8 weeks of culture before colonies can be counted visually. Here, we describe the development of luminescent M. avium subsp. paratuberculosis expressing luxAB genes of Vibrio harveyi and its use for vaccine testing in an experimental mouse model, replacing fastidious CFU counting by rapid luminometry.

Clinical Evaluation Of Keratin-Gelatin Composite Film For Wound Healing In Cats, Arul Jothi N., Thilagar S, Khaled M.A Hussin, Gayathri Thevi S, Sastry T.P

Khaled M.A Hussin

Herpes Simplex Virus Type 1 Icp0 Localizes In The Stromal Layer Of Infected Rabbit Corneas And Resides Predominantly In The Cytoplasm And/Or Perinuclear Region Of Rabbit Keratocytes, Naoyuki Morishige, James V. Jester, Julie Naito, Nelson Osorio, Andrew Wahlert, Clinton J. Jones, Roger D. Everett, Steven L. Weschler, Guey Chuen Perng

School of Veterinary and Biomedical Sciences: Faculty Publications

Herpes stromal keratitis (HSK) results from the reactivation of herpes simplex virus type-1 (HSV-1) in the cornea. The subsequent corneal inflammation and neovascularization may lead to scarring and visual loss. The cellular and molecular mechanisms underlying HSK remain unknown. The presence of stromal HSV-1 viral proteins or antigens in the HSK cornea remains a subject of debate. It was recently reported that HSV-1 ICP0 rapidly diffuses out of infected rabbit corneas. To investigate further the presence of HSV-1 ICP0 in the infected cornea, particularly in the corneal stroma, ex vivo confocal microscopy was used to scan rabbit corneas infected with …

Determinación De La Osmolaridad Y La Fracción De Excreción Renal De Sodio Y Su Posible Correlación Con La Fracción De Acortamiento Ventricular En Pacientes Cardiópatas, Lina María Parra Quijano, Catalina María Velandia Langthon

Medicina Veterinaria

Las patologías cardiacas son afecciones que comprometen el funcionamiento normal del corazón y de otros órganos del cuerpo, desencadenando problemas sistémicos que pueden llevar a la muerte del paciente a largo plazo. Debido a que son pocos los estudios de laboratorio que han valorado los electrolitos, la osmolaridad y la fracción de excreción renal de sodio en las diferentes etapas de la enfermedad cardiaca, en este proyecto se determinó la osmolaridad y la fracción de excreción de sodio buscando una posible correlación con la fracción de acortamiento ventricular en pacientes con patologías cardiacas y con esto desarrollar modelos de evaluación …

Does Diving Limit Brain Size In Cetaceans?, Lori Marino, Daniel Sol, Kristen Toren, Louis Lefebvre

Veterinary Science and Medicine Collection

We test the longstanding hypothesis, known as the dive constraint hypothesis, that the oxygenation demands of diving pose a constraint on aquatic mammal brain size.Using a sample of 23 cetacean species we examine the relationship among six different measures of relative brain size, body size, and maximum diving duration. Unlike previous tests we include body size as a covariate and perform independent contrast analyses to control for phylogeny. We show that diving does not limit brain size in cetaceans and therefore provide no support for the dive constraint hypothesis. Instead, body size is the main predictor of maximum diving duration …

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message [Page] 1- Quality System Laundry Diagnostic News [Page] 1- Submission of Rabies Specimens: SDSU ADRDL [Page] 2- Equine Herpesvirus Infections – More widespread or more recognized [Page] 4- Calving Date Variation in Beef Cows: An Illustration [Page] 5- Natural Organic Beef [Page] 7- Natural Beef in the Feedlot: Risk and Return to Feeder Calf Premiums [Page] 8- Calendar of Events

Bovine Herpesvirus 1 Immediate-Early Protein (Bicp0) Interacts With The Histone Acetyltransferase P300, Which Stimulates Productive Infection And Gc Promoter Activity, Yange Zhang, Yunquan Jiang, Vicki Geiser, Joe Zhou, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

The immediate-early protein, bICP0, of Bovine herpesvirus 1 (BHV-1) transactivates viral promoters and stimulates productive infection. bICP0 is expressed constitutively during productive infection, as its gene contains an immediate- early and an early promoter. Like other ICP0 homologues encoded by members of the subfamily Alphaherpesvirinae, bICP0 contains a zinc RING finger located near its N terminus. Mutations that disrupt the bICP0 zinc RING finger impair its ability to activate transcription, stimulate productive infection, inhibit interferon-dependent transcription in certain cell types and regulate subnuclear localization. bICP0 also interacts with a cellular chromatin-remodeling enzyme, histone deacetylase 1 (HDAC1), and can relieve …

Effects Of Moraxella (Branhamella) Ovis Culture Filtrates On Bovine Erythrocytes, Peripheral Mononuclear Cells, And Corneal Epithelial Cells, Henry E. Cerny, Douglas G. Rogers, Jeffrey T. Gray, David R. Smith, Susanne Hinkley

School of Veterinary and Biomedical Sciences: Faculty Publications

Infectious bovine keratoconjunctivitis (IBK) is a highly contagious ocular disease that affects cattle of all ages and that occurs worldwide. Piliated hemolytic Moraxella bovis is recognized as the etiologic agent of IBK. According to data from the Nebraska Veterinary Diagnostic Laboratory System, however, Moraxella (Branhamella) ovis has been isolated with increasing frequency from cattle affected with IBK. The objective of this study was, therefore, to examine M. ovis field isolates for the presence of the putative virulence factors of M. bovis. Culture filtrates from selected M. ovis field isolates demonstrated hemolytic activity on bovine erythrocytes and cytotoxic activity on …

The Investigation Of Soluble Microbial Products In Membrane Fouling, Chackrit Nuengjamnong

The Thai Journal of Veterinary Medicine

The investigation of the relative roles of Soluble Microbial Products (SMP) in membrane fouling was studied in submerged membrane bioreactors (SMBRs). Three lab-scale SMBRs were operated at a constant permeate flux (12.5 l/m2 h) with a flat sheet microfiltration membrane (hydrophilic polyolefin, pore size of 0.25 μm) using different sludge retention times (SRTs of 8, 20 and 80 days, respectively). The concentrations of SMP in terms of dissolved organic carbon (DOC) and protein at the steady-state period were compared. The results showed that as SRT increased, the organic carbon content of SMP tended to be independent of SRT while the …

The Variations And Patterns Of Renal Arteries In Dogs, Kriengyot Sajjarengpong, Adisorn Adirektaworn

The Thai Journal of Veterinary Medicine

288 kidneys complete with renal arteries and abdominal aorta, were collected from 144 dogs (75 males and 69 females), dissected and studied according to the number, branch, length and position of the renal arteries bifurcation. The positions of the left and right renal arteries were also observed. Double renal arteries were noted in 14 of 144 dogs (9.72%) but only on the left side. Single renal arteries had one to six branches. Most kidneys had 2 (39.42%) or 3 (33.58%) branches. The bifurcation mostly occurred in the middle of the renal artery (60.61%). Only ten kidneys (3.65%) were supplied by …

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.

Proceedings Ann. Con. Vet. Sci. Chula. Meeting/27-28 April, 2006., Ornsiri Cheunsuang

The Thai Journal of Veterinary Medicine

No abstract provided.