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Fully Automated Synthesis Of (Phospho)Peptide Arrays In Microtiter Plate Wells Provides Efficient Access To Protein Tyrosine Kinase Characterization., Carl Saxinger, Thomas P. Conrads, David J. Goldstein, Timothy D. Veenstra

Pharmaceutical Sciences Faculty Publications

BACKGROUND: Synthetic peptides have played a useful role in studies of protein kinase substrates and interaction domains. Synthetic peptide arrays and libraries, in particular, have accelerated the process. Several factors have hindered or limited the applicability of various techniques, such as the need for deconvolution of combinatorial libraries, the inability or impracticality of achieving full automation using two-dimensional or pin solid phases, the lack of convenient interfacing with standard analytical platforms, or the difficulty of compartmentalization of a planar surface when contact between assay components needs to be avoided. This paper describes a process for synthesis of peptides and phosphopeptides …