Medicine and Health Sciences Commons^™

A View Of Hiv--Infection In Karachi, Naila Kayani, Aamir Sheikh, Asif Khan, Chhotoobhai Mithani, Mohammad Khurshid

Department of Pathology and Laboratory Medicine

A prospective study on the prevalence of HIV-l infection in Karachi, Pakistan was conducted over a period of six years (1986-1992). Over 15,000 individualsamples and more than 32,000 donor units of individuals residing in Karachi at the time of sample co llection were tested for HIV-I infection by our screening test EIA which revealed a positivity rate of 0.23% and 0.003% in individual and donor units respectively by Western Blot. We divided patients into four groups A,B,C and D based on the most plausible cause of transmission. The largest number of positive patients belonged to group B, who were of …

Pattern Of Dyslipoproteinemia In Selected Population Of Karachi, Erum Khan, Ayesha Molla, Naila Kayani, Mohammad Khurshid

Department of Pathology and Laboratory Medicine

Pattern of dyslipoproteinemia was studied ,over a period of 3-1/2 years in 487(394 males, 93 females) cases aged 4 to above 70 years. Type IV hyperlipoproteinemia was most prevalent (36%) followed by type V (15%)

Interaoperative Frozen Section Consultation: An Analysis Of Accuracy In A Teaching Hospital., Farrukh Aijaz, Suhail Muzaffar, Akbar Shah Hussainy, Shahid Pervez, Sheema H. Hasan, Hizbullah Sheikh

Department of Pathology and Laboratory Medicine

This Is a retrospective quality assurance study of all frozen sections done at The Aga Khan University Hospital during a six year period (1986 to 1991). There were 1,031 frozen sections out of a cumulative total of 42,985 surgical specimens (2.39%). Nine hundred and seventy-six (94.66%) were concordant. In 92(8.9%) fresh specimens were brought from other hospitals of Karachi, in 37 cases (3.58%) the diagnosis was deferred till the evaluation of permanent paraffin sections and 18 (1,74%) were discordant with 7 (0.67%) false positive and 11(1,06%) false negative. Among the discordant cases, 9 were attributed to misinterpretation, 7 due to …

Does Red Blood Cell Distribution Width (Rdw) Improve Evaluation Of Microcytic Anaemias?, Muhammad Sajjad Baqar, Mohammad Khurshid, A Molla

Department of Pathology and Laboratory Medicine

Abstract

The red cell distribution width (RDW) is an index of the variation in red cells size (anisocytosis). A study was conducted to examine the validity of using RDW in improving classification of microcytic anaemias. A total of 300 blood samples collected from a patient population aged 3 months to 55 years who were referred for haemoglobin electrophoresis were examined at The Aga Khan University Hospital (AKUH). On complete blood count, initially 200 patients (66.6%) were found to have hypochromic microcytic anaemia. Following haemoglobin electrophoresis 41% (821200) patients were diagnosed to have thalassemia minor and 59% (1181200) had hypochromic microcytic …

Suggested Reference Ranges In Clinical Chemistry For Apparently Healthy Males And Females Of Pakistan., Ayesha Molla, Mohammad Khurshid, William T. Manser, Rukhsana Lalani, Anis Alam, Zubaida Mohammad

Department of Pathology and Laboratory Medicine

Abstract

Seven hundred and eighty six apparently healthy males (418) and females (368) aged 0-69 years were randomly selected for estimation of reference ranges of 24 serum analytes at the clinical chemistry laboratory of The Ago Khon University Hospital (AKUH). Of the total study samples, 56% (439/786) were in the poediatric age group (0-14 years) and 44% (347/786) in the adult (1 5_60 years) group. Beckman Astra Ideal Autoanalyzer was used for all the estimations. Moon and standard deviations (SD) were calculated for each of the age groups. Reference ranges were calculated following standard methods of the International Federation of …

Aggressive Angiomyxoma Of Vulva, Farrukh Aijaz, Shahid Pervez, Shehnaz Wasti, Sheema H. Hassan

Department of Pathology and Laboratory Medicine

No abstract provided.

Prevalence Of Thalassemia Minor Trait In Pakistani Population Presented At Akuh For Complete Blood Count Estimation (Cbc)., Alia Shaikh, Mohammad Khurshid

Department of Pathology and Laboratory Medicine

No abstract provided.

Salmonella Hadar Pericarditis, A Aziz, W Jafri, T A. Jawed, A Shaikh, B Farooqui, M K. Ashfaq, N Ul Haq

Department of Pathology and Laboratory Medicine

No abstract provided.

Factor Xiii Deficiency In Pakistan, Aamir Nadeem Shaikh, Mohammad Khurshid

Department of Pathology and Laboratory Medicine

Patients with undiagnosed haemostatic defects seen at The Aga Khan Hospital and Fatimid Blood Transfusion Centre during the period of 7 years (1 985-1 992) were screened with routine tests including bleeding time (BT), whole blood clotting time (CT), platelet count, activated partial thromboplastin time (APTT), prothrombin time (PT) and 5 molar urea test. Nine patients had a positive 5 molar urea test indicating factor XIII deficiency. Rest of the screening tests were normal in these patients. High incidence of consanguinity was observed in affected families. Clinical features included excessive bleeding from umbilical stump, bruising, post-traumatic bleeding, epistaxis, melaena and …

Malabsorption Of Nutrients In Children With Diarrhoea Due To Unknown Aetiologies, Ayesha Molla, Mohammad Khurshid, Abdul Majid Molla, Makhduma Khatun

Department of Pathology and Laboratory Medicine

Intake and coefficient of absorption of nutrients wefe measured in 72 children dufing acute diarrhoea and 2 weeks after fecovery. No diarrhooeal pathogens could be identified in 18 (25%) childfen (group I). Aetiology of diafrhoea was identified in fest of the 54 children (group II). Absofption of oalofie, fat and cafbohydrate during the recovery stage were similaf in all 72 children, In group I, absorption of nutrients improved from acute stage to recovery stage as follows: fat from 62 to 85%, calofies from 68 to 86% and carbohydrate from 81 to 91%. Absorption of nitrogen during the acute stage was …

Cysticercosis In A Muslim Woman, Farrukh Aijaz, Altaf Ahmed

Department of Pathology and Laboratory Medicine

No abstract provided.

Prevalence Of Iron Deficiency Anaemia In Children Of The Urban Slums Of Karachi, Ayesha Molla, Mohammad Khurshid, A. Majid Molla

Department of Pathology and Laboratory Medicine

A preliminary baseline survey was conducted to estimate the prevalence of anaemia in a group of 391 children aged 6-60 months, randomly selected from three urban slums of Karachi. Haemoglobin and the red cell indices including haematocrit, MCV, MCH, MCHC, ABC and red cell distribution width (RDW) were estimated for each of the selected children. Ferritin estimation was done on 354 (9 1%) children to assess the iron storage status. According to WHO criteria, the accepted cut-off point for anaemia screening in children is set at 11 gm/dl, 70 fI and 20 pg for haemoglobin, MCV and MCH respectively. Following …

Hepatic Dysfunction In Falciparum Malaria, J Khan, J Akhter, H Sheikh, W Jafri

Department of Pathology and Laboratory Medicine

No abstract provided.

Serum Alkaline Phosphatase In Apparently Healthy Karachi Population, Ayesha Molla, Rukhsana Lalani, Mohammad Khurshid, Anis Alam, William Wt. Manser

Department of Pathology and Laboratory Medicine

Serum alkaline phosphatase (AP) was estimated in a total of 786(418 males and 386 females) apparently healthy people aged between 1-75 years selected randomly from a sample of the Karachi population. Reference ranges for AP level were obtained for the males and females stratified into ten successive age groups. The study population was also divided into two main age groups, a paediatric aged between 1-14 years and an adult group aged between 15 to over 50 years to see if the mean AP levels differ significantly between the two groups. Mean AP level for the male paediatric age group was …

Urinary Tract Infection, B J Farooqui, Mehboob Alam, Mohammad Khurshid

Department of Pathology and Laboratory Medicine

Over two years, 9892 mid-stream urine samples from patients attending the Aga Khan University Hospital, Karachi were cultured. Significant bacterial growth was seen in 23.5% samples. Further iden­tification of these organisms Eevealed 40% of E.coli, 16% Pseudomonas aeruginosa, 11% Klebsiella aerogenes, 5.0% Enterobacter sp., 13% Protdus sp., 4.0% Serratia liquifaciens, 1.0% Acinetobacter sp., 3.0% Citrobacter sp., 4.0% Enterococci, 0.5% Staphylococcus aureus. Results of sensitivity tests performed with antibiotics Ampicillin, Cotrimoxa.zole, Nitrofurantoin, Nalidixic acid, Gentamicin, Amikacin, Pipemedic acid, Cefotaxime, Azactain and Carbenicillin did not reveal any distinct pattern

Efficacy Of Internal And External Quality Control In Chemical Pathology, Rukhsana Lalani, Mirza Naqi Zafar, Mohammad Khurshid

Department of Pathology and Laboratory Medicine

Quality control in chemical pathology is based on internal and external quality assessment. The internal quality control in clinical chemistry section of AKUH laboratory is based on the analysis of commercially available Beckman norma! and abnormal range control sera. These have known concentra­tions of blood chemistry constituents. After repeated estimations, mean values of all constituents were established for both normal and abnormal sera. Patients samples were analysed only when both controls were within ± 2SD from the mean or one control reads within ± 2SD and the other read between ± 2—3SD (once only) for each constituent. Samples were not …