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Full-Text Articles in Medicine and Health Sciences
In Vitro Haematopoiesis Of A Novel Dendritic-Like Cell Present In Murine Spleen, Jonathan Tan, Helen O'Neill
In Vitro Haematopoiesis Of A Novel Dendritic-Like Cell Present In Murine Spleen, Jonathan Tan, Helen O'Neill
Jonathan Tan
Abstract: Dendritic cells (DC) are important antigen presenting cells (APC) which induce and control the adaptive immune response. In spleen alone, multiple DC subsets can be distinguished by cell surface marker phenotype. Most of these have been shown to develop from progenitors in bone marrow and to seed lymphoid and tissue sites during development. This study advances in vitro methodology for haematopoiesis of dendritic-like cells from progenitors in spleen. Since spleen progenitors undergo differentiation in vitro to produce these cells, the possibility exists that spleen represents a specific niche for differentiation of this subset. The fact that an equivalent cell …
Spleen As A Distinct Site For Dendritic Cell Haematopoiesis, Jonathan Tan, Helen O'Neill
Spleen As A Distinct Site For Dendritic Cell Haematopoiesis, Jonathan Tan, Helen O'Neill
Jonathan Tan
No abstract provided.
Characterisation Of The Effect Of Lps On Dc Subset Discrimination In Spleen., Kristin Griffiths, Jonathan Tan, Helen O'Neill
Characterisation Of The Effect Of Lps On Dc Subset Discrimination In Spleen., Kristin Griffiths, Jonathan Tan, Helen O'Neill
Jonathan Tan
The Gram-negative bacterial endotoxin lipopolysaccharide (LPS) is a potent inflammatory mediator and a leading cause of bacterial sepsis. While LPS is known to activate antigen-presenting cells, here we find that LPS down-regulates expression of CD11c and CD11b on splenic dendritic cell subsets, thus confounding the ability to identify these subsets following treatment. This has implications with regard to tracking the response to LPS in terms of the cell subsets involved, and should be considered whenever such studies are undertaken.
Investigation Into The Prevalence Of A Novel Dendritic-Like Cell Subset In Vivo, Kristin Griffiths, Jonathan Tan, Helen O'Neill
Investigation Into The Prevalence Of A Novel Dendritic-Like Cell Subset In Vivo, Kristin Griffiths, Jonathan Tan, Helen O'Neill
Jonathan Tan
A novel dendritic-like cell subset termed L-DC was recently identified in murine spleen based on marker expression of a homogeneous cell population derived from long-term culture of neonatal spleen. The function of L-DC is distinct from other splenic dendritic and myeloid cell subsets because of their high endocytic capacity and their ability to cross-present antigen to CD8+ T cells. This paper shows the subset to be unique to spleen and blood, with a similar, but possibly functionally distinct subset also present in bone marrow. The prevalence of the subset is low; ~6% of all dendritic and myeloid cells in the …
Myelopoiesis In Spleen Producing Distinct Dendritic-Like Cells, Jonathan Tan, Helen O'Neill
Myelopoiesis In Spleen Producing Distinct Dendritic-Like Cells, Jonathan Tan, Helen O'Neill
Jonathan Tan
Dendritic cells (DC) represent a heterogeneous class of antigen presenting cells (APC). Previously we reported a distinct myeloid dendritic-like cell present in spleen, as an in vivo counterpart to cells produced in murine spleen long-term cultures (LTC-DC). These cells, named 'L-DC', were found to be functionally and phenotypically distinct from conventional (c)DC, plasmacytoid (p)DC and monocytes. These results suggested that spleen may represent a niche for development of L-DC from endogenous progenitors. Adult murine spleen has now been investigated for the presence of L-DC progenitors. Lineage-negative (Lin)-ckitlo and Lin-ckithi progenitor subsets were identified as candidate populations, and tested for ability …
Identification Of A Novel Antigen Cross-Presenting Cell In Spleen: A Counterpart To Cells Produced In Long-Term Culture, Jonathan Tan, Ben Quah, Kristin Griffiths, Pravin Periasamy, Yingying Hey, Helen O'Neill
Identification Of A Novel Antigen Cross-Presenting Cell In Spleen: A Counterpart To Cells Produced In Long-Term Culture, Jonathan Tan, Ben Quah, Kristin Griffiths, Pravin Periasamy, Yingying Hey, Helen O'Neill
Jonathan Tan
Antigen-presenting cells (APC), like dendritic cells (DC), are essential for T-cell activation, leading to immunity or tolerance. Multiple DC subsets each play a unique role in the immune response. Here, a novel splenic dendritic-like APC has been characterized in mice that has immune function and cell surface phenotype distinct from other, described DC subsets. These were identified as a cell type continuously produced in spleen long-term cultures (LTC) and have anin vivoequivalent cell type in mice, namely 'L-DC'. This study characterizes LTC-DC in terms of marker phenotype and function, and compares them with L-DC and other known splenic DC and …