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Medicine and Health Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Medical Sciences

Western Kentucky University

2000

Articles 1 - 3 of 3

Full-Text Articles in Medicine and Health Sciences

Morphological, Molecular, And Biogeographical Variation Within The Imperiled Virginia Spiraea, Constance Anders Aug 2000

Morphological, Molecular, And Biogeographical Variation Within The Imperiled Virginia Spiraea, Constance Anders

Masters Theses & Specialist Projects

Spiraea virginiana Britton is a rare federally listed rhizomatous shrub endemic to the southern Blue Ridge and Appalachian Plateau physiographic provinces. Populations of S. virginiana are found restricted to scoured sections of high gradient streams within the Ohio River drainage. Present evidence indicates the species is reproducing asexually, most probably through the deposition of rhizomes from upstream populations forming new downstream ramets. Phenotypic variation was examined through a morphometric evaluation of 25 leaf measurements and analyzed using Principal Components Analysis (PCA) and discriminant function analysis. Identity and structure at the molecular level as examined with Randomly Amplified Polymorphic DNA (RAPDs) …


Gene Inhibition Using Genetic Suppressor Elements In Escherichia Coli Jm109de3 Cells, Ronald Chuke May 2000

Gene Inhibition Using Genetic Suppressor Elements In Escherichia Coli Jm109de3 Cells, Ronald Chuke

Masters Theses & Specialist Projects

Genetic suppressor elements (GSEs) are short biologically active gene fragments that encode inhibitory antisense RNAs or truncated proteins that function as negative dominants. GSEs can be constructed from a single gene or isolated from a multigene library consisting of short random fragments of the target gene or genes. The goal of this study was to determine if gene inhibition in bacterial cells could be achieved by isolating GSEs from a genomic library. Additionally, if it was possible to use GSEs to inhibit gene function in E. coli JM109DE3 cells, could this method be used to locate and determine the function …


Density And Age Distributions Of Trees In Upland And Lowland Oldfields 60 Years After Abandonment: The Role Of Dispersal, Virginia Mcdaniel May 2000

Density And Age Distributions Of Trees In Upland And Lowland Oldfields 60 Years After Abandonment: The Role Of Dispersal, Virginia Mcdaniel

Masters Theses & Specialist Projects

I attempted to determine whether the patterns of seedling establishment described in the literature for forests and recently abandoned oldfields are maintained in fields sixty years after abandonment. To determine this distribution pattern, as well as to compare patterns of age and density distribution in upland and lowland oldfields, I conducted research at Mammoth Cave National Park. I studied three species, the seeds of which are dispersed by three different modes: Juniperus virginiana, a predominantly bird-dispersed species; Acer rubrum, a predominantly winddispersed species; and Fagus grandifolia, a predominantly mammal-dispersed species. The establishment patterns observed by others in recently abandoned oldfields …