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Nuclear And Chloroplast Dnas Reveal Diverse Origins And Mis-Identifications Of Juniperus Cultivars From Windsor Gardens, Uk, Part 3 Of 3, Robert P. Adams, Sam T. Johnson, John Anderson, Keith Rushforth, Nicolas Valentin, Andrea E. Schwarzbach, Sonja Siljak-Yakovlev Sep 2020

Nuclear And Chloroplast Dnas Reveal Diverse Origins And Mis-Identifications Of Juniperus Cultivars From Windsor Gardens, Uk, Part 3 Of 3, Robert P. Adams, Sam T. Johnson, John Anderson, Keith Rushforth, Nicolas Valentin, Andrea E. Schwarzbach, Sonja Siljak-Yakovlev

Health & Biomedical Sciences Faculty Publications and Presentations

Ploidy was determined for 15 plants labeled as Juniperus squamata at the Windsor Gardens, UK and revealed 12 were tetraploids (2n=4x=44) and 3 were diploids (2n=2x=22). nrDNA (ITS) and cp DNA sequencing the tetraploids found: 4 J. squamata (4x); 4 J. tibetica (4x) x J. squamata (4x); 2 J. sabina var. balkanensis (4x) x J. squamata (4x); and one J. chinensis var. sargentii (4x) x J. squamata (4x). Sequencing the 3 diploids revealed: 2 J. pingii (2x) x J. pingii (2x); and 1 J. pingii (2x)? x J. komarovii(2x)? Ploidy analyses of 18 additional cultivars, putatively from Juniperus davurica, J. …


First Molecular Characterization Of The Facultative Myiasis Agent Eristalis Tenax In Turkey Using Mitochondrial Cytochrome C Oxidase Subunit I Dna Barcoding Sequences, Mübeccel Okur, Alparslan Yildirim, Abdullah İnci̇ Jan 2020

First Molecular Characterization Of The Facultative Myiasis Agent Eristalis Tenax In Turkey Using Mitochondrial Cytochrome C Oxidase Subunit I Dna Barcoding Sequences, Mübeccel Okur, Alparslan Yildirim, Abdullah İnci̇

Turkish Journal of Veterinary & Animal Sciences

This report is the first molecular characterization of the facultative myiasis agent Eristalis tenax collected from cattle barns in Kayseri province of Central Anatolia, Turkey. A total of 10 adult fly specimens were included in the study. All flies were identified as female adults of E. tenax by morphologic characteristics under a stereomicroscope. The barcode region of the mitochondrial cytochrome oxidase 1 (CO1) gene was amplified with PCR from the genomic DNA extracts of E. tenax specimens for molecular identification and phylogenetic analyses. There was no polymorphism among the CO1 sequences of all isolates leading to the detection of a …