Medicine and Health Sciences Commons^™

Genomic Diversity Of Pseudomonas Aeruginosa In Cystic Fibrosis, Nathaniel Edwards Head

Theses, Dissertations and Capstones

Pseudomonas aeruginosa is a common environmental microorganism. However, it has the genetic capacity to cause diseases in patients with compromised host defense systems. Cystic fibrosis (CF) is one of the major hereditary diseases among Caucasian populations. CF patients are born with a defective chloride channel that is responsible for maintaining fluid and electrolyte balance across the lumen of the lung. This imbalance leads to the production of an abnormal dehydrated viscous mucous, prohibiting the patient from normally clearing the respiratory airway. Chronic pulmonary infections with P. aeruginosa are the major causes of high morbidity and mortality in CF. Two of …

An Investigation Of The Prevalence Of Disordered Eating Behaviors In Dietetic Students, Brandi Elizabeth Hicks

Theses, Dissertations and Capstones

Eating disorders are a debilitating mental illness that affects young people including those in the field of dietetics. Literature suggests that dietetic students have a higher prevalence of eating disorders than other students. Seventy-seven college student in Southern West Virginia enrolled in either dietetics or another major were included in a sample to determine if dietetic students have higher prevalence of disordered eating attitudes and behaviors. The participants completed a survey that included a general information section, a 26-item questionnaire concerning eating attitudes, and a behavioral information section. Upon completion of data collection, the survey was coded, and data was …

Isokinetic Dynamometer Versus A Multi-Axial Stability Platform In The Proprioception And Strength Training Of The Peroneal Muscle Group, Eric Johnson

Theses, Dissertations and Capstones

Ankle injuries are the most common and frequent occurring injuries in sports today (Feuerbach, Grabiner & Koh, 1994). Ankle sprains usually occur in athletes that are participating in running or jumping activities. Injuries to the ankle joint represent about 20% of all sports related injuries, and 15% of all time lost injuries (Payne, Berg, & Latin, 1997). The majority of the sprains to the ankle occur to the lateral ligamentous structures due to the forcefully plantar flexion and inversion of the talocrural joint. This inversion mechanism accounts for 85% of all ankle sprains (Kinzey, Ingersoll, & Knight, 1997). Often after …

Methods Development For The Identification Of Unknown Proteins, Laura Brumfield Neal

Theses, Dissertations and Capstones

The chemistry of transport, storage, energy production, and reproduction in physiological systems is controlled by the actions and interactions of proteins. A species full complement of these amazing machines is often referred to as its proteome. The new science of proteomics utilizes a set of powerful and evolving tools to characterize the nature of the proteome, and further to fully elucidate the functional attributes of the proteins and their associations.

A methodology was developed by combining several proteomic techniques, such as electrophoresis, RP-HPLC, and mass spectrometry, to correctly identity proteins. Three known proteins (myoglobin, albumin, and actin) were employed for …

Effect Of Forskolin On Actin Polymerization In Cultured Vascular Smooth Muscle, Mary Janay Bailey

Theses, Dissertations and Capstones

Increases in intracellular cyclic AMP levels have been shown to affect vascular smooth muscle morphology. In the present study, effects of elevated cyclic AMP levels on actin polymerization were assessed to determine whether cyclic AMP induces specific changes in cytoskeletal proteins of vascular smooth muscle cells. Forskolin was used to increase intracellular cyclic AMP levels through direct activation of adenylate cyclase. Light and Electron microscopy was employed to appraise changes in the shape of vascular smooth muscle cells. Confocal microscopy was used to reveal cytoskeletal changes after exposure to M forskolin for increasing intervals of time. Differences in the expression …