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Full-Text Articles in Medicine and Health Sciences
Identification Of Proteins Involved In Cell Membrane Permeabilization By Nanosecond Electric Pulses (Nsep), Giedre Silkuniene, Uma Mangalanathan, Alessandra Rossi, Peter A. Mollica, Andrei G. Pakhomov, Olga N. Pakhomova
Identification Of Proteins Involved In Cell Membrane Permeabilization By Nanosecond Electric Pulses (Nsep), Giedre Silkuniene, Uma Mangalanathan, Alessandra Rossi, Peter A. Mollica, Andrei G. Pakhomov, Olga N. Pakhomova
Bioelectrics Publications
The study was aimed at identifying endogenous proteins which assist or impede the permeabilized state in the cell membrane disrupted by nsEP (20 or 40 pulses, 300 ns width, 7 kV/cm). We employed a LentiArray CRISPR library to generate knockouts (KOs) of 316 genes encoding for membrane proteins in U937 human monocytes stably expressing Cas9 nuclease. The extent of membrane permeabilization by nsEP was measured by the uptake of Yo-Pro-1 (YP) dye and compared to sham-exposed KOs and control cells transduced with a non-targeting (scrambled) gRNA. Only two KOs, for SCNN1A and CLCA1 genes, showed a statistically significant reduction in …
Genética Del Glaucoma Primario De Ángulo Abierto, Vivian Alexandra Gutiérrez Carranza, Marcelo Carrizosa Murcia
Genética Del Glaucoma Primario De Ángulo Abierto, Vivian Alexandra Gutiérrez Carranza, Marcelo Carrizosa Murcia
Ciencia y Tecnología para la Salud Visual y Ocular
Objetivo conocer la genética del glaucoma primario de ángulo abierto para la identificación de genes que permitan establecer una herramienta de diagnóstico temprano para un mejor manejo de la enfermedad. Materiales y métodos se realizó una búsqueda sistematizada de literatura primaria en bases de datos como PubMed, SciELO y Elsevier además, se utilizaron referentes de varios textos para algunas terminologías, cuadros y tablas de los diferentes glaucomas, para determinar las características, el diagnóstico diferencial y la identificación de los genes asociados con el desarrollo del glaucoma primario de ángulo abierto. La información seleccionada corresponde al periodo 2010-2015. Conclusiones el glaucoma …
Improved Tumor Contrast Achieved By Single Time Point Dual-Reporter Fluorescence Imaging, Kenneth M. Tichauer, Kimberley S. Samkoe, Kristian J. Sexton, Jason R. Gunn, Tayyaba Hasan, Brian W. Pogue
Improved Tumor Contrast Achieved By Single Time Point Dual-Reporter Fluorescence Imaging, Kenneth M. Tichauer, Kimberley S. Samkoe, Kristian J. Sexton, Jason R. Gunn, Tayyaba Hasan, Brian W. Pogue
Dartmouth Scholarship
In this study, we demonstrate a method to quantify biomarker expression that uses an exogenous dual-reporter imaging approach to improve tumor signal detection. The uptake of two fluorophores, one nonspecific and one targeted to the epidermal growth factor receptor (EGFR), were imaged at 1 h in three types of xenograft tumors spanning a range of EGFR expression levels (n = 6 in each group). Using this dual-reporter imaging methodology, tumor contrast-to-noise ratio was amplified by >6 times at 1 h postinjection and >2 times at 24 h. Furthermore, by as early as 20 min postinjection, the dual-reporter imaging signal …