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Full-Text Articles in Medicine and Health Sciences

The Effect Of Mtad On Eight Strains Of E. Faecalis In Contaminated Root Canals, Bradley M. Newberry Aug 2007

The Effect Of Mtad On Eight Strains Of E. Faecalis In Contaminated Root Canals, Bradley M. Newberry

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this investigation was to determine the antimicrobial effect of Biopure MTAD (a mixture of a tetracycline isomer, an acid, and a detergent) as a final irrigant on eight strains of Enterococcus faecalis (E. faecalis) in contaminated root canals of extracted human teeth. In addition, the Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) of MTAD were also measured on the same strains of bacteria. Two-hundred and forty extracted human teeth were instrumented using 1.3% NaOCl followed by 17% EDTA to remove the smear layer. The teeth were randomly contaminated with one of eight strains of E. …


Detoxification Of Lipopolysaccharide By Endodontic Sealers, Dominic Côté May 2007

Detoxification Of Lipopolysaccharide By Endodontic Sealers, Dominic Côté

Loma Linda University Electronic Theses, Dissertations & Projects

Bacterial lipopolysaccharide (LPS) has been implicated in the pathogenesis of pulpal and periapical diseases. Several studies have evaluated the ability of various medicaments and irrigants used during root canal therapy to chemically detoxify LPS. The purpose of this study was to evaluate the ability of some endodontic sealers to detoxify LPS. This investigation compared the quantities of TNF-α released by human monocytes exposed to LPS or LPS pre-treated with sealers. The sealers tested were: Pulp Canal Sealer EWT (PCS), AH Plus (AH), and Sealapex (SA). Ca(OH)2 and water (H2O) were respectively used as the positive and negative control materials. …


Warm Vertical Compaction Versus Lateral Compaction For Root Canal Obturation, Ahmed A. Madfa Jan 2007

Warm Vertical Compaction Versus Lateral Compaction For Root Canal Obturation, Ahmed A. Madfa

Ahmed A. Madfa

Objectives: This study compared warm vertical compaction with lateral compaction for obturating root canals with either a polymer-based resin system or gutta-percha. Quality of obturation was evaluated by (i) analysis of the canal area occupied by filling core materials, sealers and voids at three cross-sectional levels and (ii) SEM examination. Methods: Single canals in 64 mandibular premolars were instrumented, irrigated and divided into four equal groups. They were root filled as follows: lateral compaction/resin (RealSeal™, SybronEndo) (LC/R), lateral compaction/gutta-percha with AH Plus™ (LC/GP), warm compaction/resin (WC/R) and warm compaction/gutta-percha (WC/GP). One specimen from each group, randomly chosen for SEM examination, …


A Comparison Of Flexural Fracture Of Three Different Nickel-Titanium Rotary File Systems, Matthew Warren Lloyd Jan 2007

A Comparison Of Flexural Fracture Of Three Different Nickel-Titanium Rotary File Systems, Matthew Warren Lloyd

Theses and Dissertations

The purpose of this study was to compare the number of rotations to failure of three different rotary file systems. ProFile, Sequence, and Liberator files in sizes 25 and 40 with 0.04 taper were divided into groups of five and rotated against a grooved metal block mounted to a Universal testing machine at 31 and 34 degrees. Each file was rotated at 300 rpm until fracture occurred. The number of rotations to fracture were calculated. Use of a three-way ANOVA and Tukey's HSD multiple comparison tests revealed significant differences for the angle of deflection, size, and type of file. An …


Sodium Hypochlorite's Effect On Nickel-Titanium Rotary Instruments And Its Effect On Resistance To Fracture, Michael Shane Smith Jan 2007

Sodium Hypochlorite's Effect On Nickel-Titanium Rotary Instruments And Its Effect On Resistance To Fracture, Michael Shane Smith

Theses and Dissertations

The purpose of this study was to examine the effect of partial and total immersion in sodium hypochlorite on nickel-titanium rotary files and to determine whether resistance to fracture was influenced by the immersion time. One hundred K3™ and 100 ProFile® rotary files were either partially or totally immersed in 5.25% sodium hypochlorite for zero, one, five, thirty, or sixty minutes. After immersion, files were subjected to cyclic fatigue testing. Time to fracture was recorded and analyzed by a two-way ANOVA. Tukey's honest significant difference was used to identify any differences in immersion times. Within all ProFile groups and partial …


Immunohistochemical Study Of Phenotypes Of Dendritic Cells In Dental Pulps From Non-Carious And Carious Teeth, Melissa A. Harmon Jan 2007

Immunohistochemical Study Of Phenotypes Of Dendritic Cells In Dental Pulps From Non-Carious And Carious Teeth, Melissa A. Harmon

Theses and Dissertations

Mature dendritic cells (DCs) in inflamed tissues may promote inflammation but the status of DCs in pulpitis is not known. We hypothesized that DC maturation would correlate with carious lesion depth and that CD4+ cells would be found in association with mature DCs. Pulps were collected from teeth exhibiting: (I) no caries (n=9), (II) shallow dentinal caries (n=5), and (III) deep caries (n=9). Pulpal tissues were cryo-sectioned and positive cells were examined with immunohistochemistry, Mature DCs (CD83+) were almost exclusively restricted to pulps from deep caries. Furthermore, CD209+ DCs in deep caries were elevated over other groups and CD209+ cells …


An Invitro Leakage And Viscosity Analysis Of Root End Filling Materials, Paul D. Clark Jan 2007

An Invitro Leakage And Viscosity Analysis Of Root End Filling Materials, Paul D. Clark

Theses and Dissertations

The purpose of this study was to determine if the viscosity of MTA Angelus Fluid is lower than that of Pro Root MTA and MTA Angelus; and to compare the viscosity, leakage and particle size of these materials to determine whether a relationship exists between these properties. The viscosity of each material was measured and compared with the Student's t test. MTA Angelus Fluid's viscosity was significantly lower than the other two materials tested. Microleakage of root end fillings was assessed in a passive diffusion model. Leakage groups were compared with a one-way ANOVA (p < 0.05). No significant difference was found. Particle size and shape were evaluated with the SEM. MTA Angelus Fluid has a lower viscosity than the other materials tested. There was no significant difference in the sealing ability of the three materials tested and there was no apparent variation in each material's particle size or shape.