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Ephemeroptera Culturing Methods: An Analysis On Rearing And Toxcity Testing On Sensitive Early Life Stages Of Native Mayfly Taxa, Geneve Rainette Edwards Jan 2022

Ephemeroptera Culturing Methods: An Analysis On Rearing And Toxcity Testing On Sensitive Early Life Stages Of Native Mayfly Taxa, Geneve Rainette Edwards

Theses, Dissertations and Capstones

Standard toxicity testing organisms are utilized for regulatory purposes and often ecological risk assessments. Ephemeroptera taxa have been reported to be more sensitive to aquatic contaminants than the standard toxicity testing organisms currently used in determining effects on aquatic ecosystems. Establishing methods for culturing native Ephemeroptera taxa will provide a more sensitive test organism to determine the toxicity of contaminants and will be more representative of the responses of native taxa. Additionally, it will provide a test organism at the most sensitive life stages. The objective of this research is to develop methods for culturing and testing of native Ephemeroptera …


Ephemeroptera Culturing Methods: Development Of Culturing And Rearing Methodology And High Sulfate Simulated Mine Effluent Toxicity Test Using Field Collected Water, Daniel Edward Brady Jan 2022

Ephemeroptera Culturing Methods: Development Of Culturing And Rearing Methodology And High Sulfate Simulated Mine Effluent Toxicity Test Using Field Collected Water, Daniel Edward Brady

Theses, Dissertations and Capstones

Ephemeroptera taxa are not frequently used in toxicity testing; however, some mayfly taxa may be more sensitive to aquatic pollutants than standard test organisms used to determine anthropogenic effects on aquatic ecosystems. Additionally, some standard test organisms are not native to the Appalachian region and may not be truly reflective of the effects on native organisms. With mayflies not being the typical test organism, there is not a great deal of literature on culturing methods for this organism. For a standard acute toxicity test, there must be 80% survival within the control organisms for the test to be considered viable. …