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The Role Of Brn-3.2 In Retinal Ganglion Cell Differentiation, Jerin Marie Wright
The Role Of Brn-3.2 In Retinal Ganglion Cell Differentiation, Jerin Marie Wright
Loma Linda University Electronic Theses, Dissertations & Projects
Within the retina, the POU domain of transcription factors brn-3.0, brn-3.1, and brn-3.2 are present only in retinal ganglion cells. These genes are believed to be involved in establishing neural cell lineages in mammals. In this study brn-3.2 was examined by comparing the number of ganglion cells present during postnatal development in normal mice (+/+), in mice homozygous (-/-) for the brn-3.2 gene, and in adult mice with a heterozygous gene deletion (+/-) for brn-3.2. Optic nerve cross sections were imaged by electron microscopy, and axon profiles counted systematically by hand. These counts were then related to the nerve cross …