Open Access. Powered by Scholars. Published by Universities.®
Research Methods in Life Sciences Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Research Methods in Life Sciences
Proto-Oncogene Regulation By Growth Factors In Bone Cells, Harold Lyndon Merriman
Proto-Oncogene Regulation By Growth Factors In Bone Cells, Harold Lyndon Merriman
Loma Linda University Electronic Theses, Dissertations & Projects
Previous studies have shown that bone cells in culture produce a number of growth factors that are important in bone regulation. Chick and mouse primary calvarial cultures consist of a mixture of cell types that make it difficult to interpret the results from these model systems. In contrast, MC3T3-E1 cells are a clonal mouse osteoblast-like cell line. Since MC3T3-E1 cells consist of a single population of calvarial cells, they make an ideal system in which to study the autocrine effects of bone growth factors.
Based on this work, MC3T3-E1 cells are now known to produce IGF-I, TGF-beta and IGF-II in …