Research Methods in Life Sciences Commons^™

Levels Of Intravenous Enterococcus Faecalis That Cause Heart Colonization, Louis Zane Stromberg

Loma Linda University Electronic Theses, Dissertations & Projects

While previous studies have shown that presence of bacteria in systemic circulation can cause infective endocarditis, there is no information on the specific amount of bacteria necessary to cause this condition. The purpose of this study was to establish the minimum level of circulating bacteria that will cause colonization of damaged heart valves in rats. Fifty-two Sprague-Dawley rats were anesthetized and their heart valves were damaged using an established protocol. A clinical isolate of Enterococcus faecalis was transformed with a plasmid bearing the luxF and Chloramphenicol cassettes to label the bacteria with the light-emitting protein. After four weeks, the rats …