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Maize

University of Nebraska - Lincoln

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Full-Text Articles in Plant Sciences

Life Cycle Greenhouse Gas Emissions In Maize No-Till Agroecosystems In Southern Brazil Based On A Long-Term Experiment, Guilherme Rosa Da Silva, Adam J. Liska, Cimelio Bayer Jan 2024

Life Cycle Greenhouse Gas Emissions In Maize No-Till Agroecosystems In Southern Brazil Based On A Long-Term Experiment, Guilherme Rosa Da Silva, Adam J. Liska, Cimelio Bayer

Biological Systems Engineering: Papers and Publications

Brazilian agriculture is constantly questioned concerning its environmental impacts, particularly greenhouse gas (GHG) emissions. This research study used data from a 34-year field experiment to estimate the life cycle GHG emissions intensity of maize production for grain in farming systems under no-tillage (NT) and conventional tillage (CT) combined with Gramineae (oat) and legume (vetch) cover crops in southern Brazil. We applied the Feedstock Carbon Intensity Calculator for modeling the “field-to-farm gate” emissions with measured annual soil N2O and CH4 emissions data. For net CO2 emissions, increases in soil organic C (SOC) were applied as a proxy, …


2020-2021 Field Seasons Of Maize Gxe Project Within The Genomes To Fields Initiative, Dayane Cristina Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Alden Perkins, Dylan L. Schoemaker, Martin Costa, Kathryn J. Michel, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint-Garcia, Michael A. Gore, Candice N. Hirsch, Joseph E. Knoll, John Mckay, Richard Minyo, Seth C. Murray, James Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Erin E. Sparks, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Wenwei Xu, Natalia De Leon Dec 2023

2020-2021 Field Seasons Of Maize Gxe Project Within The Genomes To Fields Initiative, Dayane Cristina Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Alden Perkins, Dylan L. Schoemaker, Martin Costa, Kathryn J. Michel, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint-Garcia, Michael A. Gore, Candice N. Hirsch, Joseph E. Knoll, John Mckay, Richard Minyo, Seth C. Murray, James Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Erin E. Sparks, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Wenwei Xu, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Objectives: This release note describes the Maize GxE project datasets within the Genomes to Fields (G2F) Initiative. The Maize GxE project aims to understand genotype by environment (GxE) interactions and use the information collected to improve resource allocation efficiency and increase genotype predictability and stability, particularly in scenarios of variable environmental patterns. Hybrids and inbreds are evaluated across multiple environments and phenotypic, genotypic, environmental, and metadata information are made publicly available. Data description: The datasets include phenotypic data of the hybrids and inbreds evaluated in 30 locations across the US and one location in Germany in 2020 and 2021, soil …


Responses Of Maize To Different Irrigation Regimes In Semi-Arid Western Nebraska, Swathi Palle Dec 2023

Responses Of Maize To Different Irrigation Regimes In Semi-Arid Western Nebraska, Swathi Palle

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

As the “Cornhusker State”, Maize (Zea mays) is an important crop in Nebraska. However, farmers in the state are challenged by unstable supply of surface water and limited groundwater resources. A better understanding of maize's response to water stress that occurs at different growth stages can help implement the best irrigation practices that conserve water while maintaining yields. In this study, we conducted field experiments at the Panhandle Research, Extension, and Education Center to compare the responses of maize to eight irrigation treatments which included both limited and deficit irrigation regimes during 2022 and 2023 growing seasons. Specifically, …


Genomes To Fields 2022 Maize Genotype By Environment Prediction Competition, Dayane Cristina Lima, Jacob D. Washburn, José Ignacio Varela, Qiuyue Chen, Joseph L. Gage, Maria Cinta Romay, James Holland, David Ertl, Marco Lopez-Cruz, Fernando M. Aguate, Gustavo De Los Campos, Shawn Kaeppler, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint-Garcia, Michael A. Gore, Candice N. Hirsch, Joseph E. Knoll, John Mckay, Richard Minyo, Seth C. Murray, Osler A. Ortez, James C. Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Erin E. Sparks, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Teclemariam Weldekidan, Wenwei Xu, Natalia De Leon Jul 2023

Genomes To Fields 2022 Maize Genotype By Environment Prediction Competition, Dayane Cristina Lima, Jacob D. Washburn, José Ignacio Varela, Qiuyue Chen, Joseph L. Gage, Maria Cinta Romay, James Holland, David Ertl, Marco Lopez-Cruz, Fernando M. Aguate, Gustavo De Los Campos, Shawn Kaeppler, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint-Garcia, Michael A. Gore, Candice N. Hirsch, Joseph E. Knoll, John Mckay, Richard Minyo, Seth C. Murray, Osler A. Ortez, James C. Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Erin E. Sparks, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Teclemariam Weldekidan, Wenwei Xu, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Objectives The Genomes to Fields (G2F) 2022 Maize Genotype by Environment (GxE) Prediction Competition aimed to develop models for predicting grain yield for the 2022 Maize GxE project field trials, leveraging the datasets previously generated by this project and other publicly available data.

Data description This resource used data from the Maize GxE project within the G2F Initiative [1]. The dataset included phenotypic and genotypic data of the hybrids evaluated in 45 locations from 2014 to 2022. Also, soil, weather, environmental covariates data and metadata information for all environments (combination of year and location). Competitors also had access to ReadMe …


2018–2019 Field Seasons Of The Maize Genomes To Fields (G2f) G X E Project, Dayane Dayane Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Bridget A. Mcfarland, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint‑Garcia, Candice N. Hirsch, Elizabeth Hood, David C. Hooker, Joseph E. Knoll, Judith M. Kolkman, Sanzhen Liu, John Mckay, Richard Minyo, Danilo E. Moreta, Seth C. Murray, Rebecca Nelson, James C. Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Randall J. Wisser, Wenwei Xu31, Natalia De Leon May 2023

2018–2019 Field Seasons Of The Maize Genomes To Fields (G2f) G X E Project, Dayane Dayane Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Bridget A. Mcfarland, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint‑Garcia, Candice N. Hirsch, Elizabeth Hood, David C. Hooker, Joseph E. Knoll, Judith M. Kolkman, Sanzhen Liu, John Mckay, Richard Minyo, Danilo E. Moreta, Seth C. Murray, Rebecca Nelson, James C. Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Randall J. Wisser, Wenwei Xu31, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Objectives This report provides information about the public release of the 2018–2019 Maize G X E project of the Genomes to Fields (G2F) Initiative datasets. G2F is an umbrella initiative that evaluates maize hybrids and inbred lines across multiple environments and makes available phenotypic, genotypic, environmental, and metadata information. The initiative understands the necessity to characterize and deploy public sources of genetic diversity to face the challenges for more sustainable agriculture in the context of variable environmental conditions.

Data description Datasets include phenotypic, climatic, and soil measurements, metadata information, and inbred genotypic information for each combination of location and year. …


Exploring Gene Expression Patterns For Resilient Maize Lines Under Nitrogen Stress, Alice Guo May 2023

Exploring Gene Expression Patterns For Resilient Maize Lines Under Nitrogen Stress, Alice Guo

Honors Theses

Nitrogen plays a major role in the proper growth and development of maize and is therefore essential to crop production, being the most critical nutrient for achieving optimal yield. In previous field and greenhouse studies, we have found maize lines that differ in their resiliency to nitrogen stress. To identify the potential genomic regions associated with the differences in nitrogen deficiency resilience, Genome-Wide Association Studies (GWAS) were conducted. Based on a previous study, the most consistently resilient maize inbred lines within the Goodman-Buckler diversity panel have been identified as the lines A619 and A661, while the non-resilient inbred lines have …


Fusarium Species Structure In Nebraska Corn, Yuchu Ma Apr 2023

Fusarium Species Structure In Nebraska Corn, Yuchu Ma

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Fusarium species are known to infect corn and cause significant yield losses and mycotoxin contamination worldwide. In this study, we investigated the diversity of Fusarium species infecting corn in Nebraska and their potential to produce fumonisins and trichothecenes. A total of 259 Fusarium isolates were collected from different corn tissues (ear, stalk, and root), revealing a significant association between the various Fusarium species complexes and different plant parts (p < 0.05). Fusarium incarnatum-equiseti species complex (FIESC) was the most widespread and abundant, followed by the Fusarium sambucinum (FSAMSC) and Fusarium fujikuroi species complexes (FFSC). In the subsequent analysis, we investigated the mycotoxin …


A Leaf-Level Spectral Library To Support High-Throughput Plant Phenotyping: Predictive Accuracy And Model Transfer, Nuwan K. Wijewardane, Huichun Zhang, Jinliang Yang, James C. Schnable, Daniel P. Schachtman, Yufeng Ge Apr 2023

A Leaf-Level Spectral Library To Support High-Throughput Plant Phenotyping: Predictive Accuracy And Model Transfer, Nuwan K. Wijewardane, Huichun Zhang, Jinliang Yang, James C. Schnable, Daniel P. Schachtman, Yufeng Ge

Department of Agronomy and Horticulture: Faculty Publications

Leaf-level hyperspectral reflectance has become an effective tool for high-throughput phenotyping of plant leaf traits due to its rapid, low-cost, multi-sensing, and non-destructive nature. However, collecting samples for model calibration can still be expensive, and models show poor transferability among different datasets. This study had three specific objectives: first, to assemble a large library of leaf hyperspectral data (n=2460) from maize and sorghum; second, to evaluate two machine-learning approaches to estimate nine leaf properties (chlorophyll, thickness, water content, nitrogen, phosphorus, potassium, calcium, magnesium, and sulfur); and third, to investigate the usefulness of this spectral library for predicting external datasets …


On Correlation Between Canopy Vegetation And Growth Indexes Of Maize Varieties With Different Nitrogen Efficiencies, Xia Zhao, Shuaili Wang, Tao Wen, Jiamin Xu, Bao Huang, Shufeng Yan, Gangqiang Gao, Yali Zhao, Hongping Li, Jiangfang Qiao, Jinliang Yang, Lianhai Wu, Hongwei Wang, Tianxue Liu, Xinyuan Mu Jan 2023

On Correlation Between Canopy Vegetation And Growth Indexes Of Maize Varieties With Different Nitrogen Efficiencies, Xia Zhao, Shuaili Wang, Tao Wen, Jiamin Xu, Bao Huang, Shufeng Yan, Gangqiang Gao, Yali Zhao, Hongping Li, Jiangfang Qiao, Jinliang Yang, Lianhai Wu, Hongwei Wang, Tianxue Liu, Xinyuan Mu

Department of Agronomy and Horticulture: Faculty Publications

Studying the canopy spectral reflection characteristics of different N-efficient maize varieties and analyzing the relationship between their growth indicators and spectral vegetation indices can help the breeding and application of N-efficient maize varieties. To achieve the optimal management of N fertilizer resources, developing N-efficient maize varieties is necessary. In this research, maize varieties, i.e., the low-N-efficient (Zhengdan 958, ZD958), the high-N efficient (Xianyu 335, XY335), the double-high varieties (Qiule 368, QL368), and the double inefficient-type varieties (Yudan 606 YD606), were used as materials. Results indicate that nitrogen fertilization significantly increased the vegetation indices NDVI, GNDVI, GOSAVI, and RVI of maize …


A Glass Bead Semi-Hydroponic System For Intact Maize Root Exudate Analysis And Phenotyping, Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares, Daniel P. Schachtman, Sophie Alvarez, Karin V. Van Dijk Dec 2022

A Glass Bead Semi-Hydroponic System For Intact Maize Root Exudate Analysis And Phenotyping, Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares, Daniel P. Schachtman, Sophie Alvarez, Karin V. Van Dijk

Department of Agronomy and Horticulture: Faculty Publications

Background: Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these systems require disruption of the plant root system to facilitate exudate collection. Here, we present a newly designed semi-hydroponic system that uses glass beads as solid support to simulate soil impedance, which combined with drip irrigation, facilitates growth of healthy maize plants, collection and analysis of root exudates, and phenotyping of the roots with minimal growth disturbance or root damage. Results: This system was used to collect root exudates from seven maize genotypes using water or 1 mM CaCl2, …


Mycorrhizae In Maize (Zea Mays L.) Cropping Systems Respond Differently To Nitrogen Fertilization Under Increasing Crop Rotational Diversity, Morgan Mcpherson Aug 2022

Mycorrhizae In Maize (Zea Mays L.) Cropping Systems Respond Differently To Nitrogen Fertilization Under Increasing Crop Rotational Diversity, Morgan Mcpherson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Arbuscular mycorrhizal fungi (AMF) remain a vital obligate symbiont of nearly all plants. It is well established that the symbiosis between AMF and host plant improves plant nutrient acquisition, alleviates abiotic and biotic environmental stressors, defends against plant pathogens, and contributes to overall plant fitness and productivity through modification of the soil habitat. Modifications include increased soil aggregation and stability, carbon sequestration through provision of fungal wall precursors to soil organic matter (SOM) formation, and enhanced nutrient cycling in the mycorrhizosphere. The goal of this dissertation was to assess how AMF respond to nitrogen (N) fertilization regimes in maize cropping …


The Pho1;2a'-M1.1 Allele Of Phosphate1 Conditions Misregulation Of The Phosphorus Starvation Response In Maize (Zea Mays Ssp. Mays L.), Ana Laura Alonso-Nieves, M. Nancy Salazar-Vidal, J. Vladimir Torres-Rodríguez, Leonardo M. Pérez-Vázquez, Julio A. Massange-Sánchez, C. Stewart Gillmor, Ruairidh J. H. Sawers Jun 2022

The Pho1;2a'-M1.1 Allele Of Phosphate1 Conditions Misregulation Of The Phosphorus Starvation Response In Maize (Zea Mays Ssp. Mays L.), Ana Laura Alonso-Nieves, M. Nancy Salazar-Vidal, J. Vladimir Torres-Rodríguez, Leonardo M. Pérez-Vázquez, Julio A. Massange-Sánchez, C. Stewart Gillmor, Ruairidh J. H. Sawers

Center for Plant Science Innovation: Faculty and Staff Publications

Plant PHO1 proteins play a central role in the translocation and sensing of inorganic phosphate. The maize (Zea mays ssp. mays) genome encodes two co-orthologs of the Arabidopsis PHO1 gene, designated ZmPho1;2a and ZmPho1;2b. Here, we report the characterization of the transposon footprint allele Zmpho1;2a'-m1.1, which we refer to hereafter as pho1;2a. The pho1;2a allele is a stable derivative formed by excision of an Activator transposable element from the ZmPho1;2a gene. The pho1;2a allele contains an 8-bp insertion at the point of transposon excision that disrupts the reading frame and is predicted to …


Association Mapping Across A Multitude Of Traits Collected In Diverse Environments In Maize, Ravi V. Mural, Guangchao Sun, Marcin Grzybowski, Michael C. Tross, Hongyu Jin, Christine Smith, Linsey Newton, Carson M. Andorf, Margaret R. Woodhouse, Addie M. Thompson, Brandi Sigmon, James C. Schnable May 2022

Association Mapping Across A Multitude Of Traits Collected In Diverse Environments In Maize, Ravi V. Mural, Guangchao Sun, Marcin Grzybowski, Michael C. Tross, Hongyu Jin, Christine Smith, Linsey Newton, Carson M. Andorf, Margaret R. Woodhouse, Addie M. Thompson, Brandi Sigmon, James C. Schnable

Center for Plant Science Innovation: Faculty and Staff Publications

Classical genetic studies have identified many cases of pleiotropy where mutations in individual genes alter many different phenotypes. Quantitative genetic studies of natural genetic variants frequently examine one or a few traits, limiting their potential to identify pleiotropic effects of natural genetic variants. Widely adopted community association panels have been employed by plant genetics communities to study the genetic basis of naturally occurring phenotypic variation in a wide range of traits. High-density genetic marker data—18M markers—from 2 partially overlapping maize association panels comprising 1,014 unique genotypes grown in field trials across at least 7 US states and scored for 162 …


Impact Of Urbanization Trends On Production Of Key Staple Crops, José F. Andrade, Kenneth G. Cassman, Juan I. Rattalino Edreira, Fahmuddin Agus, Abdullahi Bala, Nanyan Deng, Patricio Grassini Nov 2021

Impact Of Urbanization Trends On Production Of Key Staple Crops, José F. Andrade, Kenneth G. Cassman, Juan I. Rattalino Edreira, Fahmuddin Agus, Abdullahi Bala, Nanyan Deng, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Urbanization has appropriated millions of hectares of cropland, and this trend will persist as cities continue to expand. We estimate the impact of this conversion as the amount of land needed elsewhere to give the same yield potential as determined by differences in climate and soil properties. Robust spatial upscaling techniques, well-validated crop simulation models, and soil, climate, and cropping system databases are employed with a focus on populous countries with high rates of land conversion. We find that converted cropland is 30–40% more productive than new cropland, which means that projection of food production potential must account for expected …


Hyperspectral Reflectance-Based Phenotyping For Quantitative Genetics In Crops: Progress And Challenges, Marcin Grzybowski, Kuwan K. Wijewardane, Abbas Atefi, Yufeng Ge, James C. Schnable Oct 2021

Hyperspectral Reflectance-Based Phenotyping For Quantitative Genetics In Crops: Progress And Challenges, Marcin Grzybowski, Kuwan K. Wijewardane, Abbas Atefi, Yufeng Ge, James C. Schnable

Center for Plant Science Innovation: Faculty and Staff Publications

Many biochemical and physiological properties of plants that are of interest to breeders and geneticists have extremely low throughput and/or can only be measured destructively. This has limited the use of information on natural variation in nutrient and metabolite abundance, as well as photosynthetic capacity in quantitative genetic contexts where it is necessary to collect data from hundreds or thousands of plants. A number of recent studies have demonstrated the potential to estimate many of these traits from hyperspectral reflectance data, primarily in ecophysiological contexts. Here, we summarize recent advances in the use of hyperspectral reflectance data for plant phenotyping, …


Implications Of Cover Crop Planting And Termination Timing On Rainfed Maize Production In Semi-Arid Cropping Systems, Alexandre T. Rosa, Cody Creech, Roger W. Elmore, Daran Rudnick, John L. Lindquist, Miguel Fudolig, Liberty E. Butts, Rodrigo Werle Sep 2021

Implications Of Cover Crop Planting And Termination Timing On Rainfed Maize Production In Semi-Arid Cropping Systems, Alexandre T. Rosa, Cody Creech, Roger W. Elmore, Daran Rudnick, John L. Lindquist, Miguel Fudolig, Liberty E. Butts, Rodrigo Werle

Department of Agronomy and Horticulture: Faculty Publications

Despite the potential to increase soil organic matter, cycle soil nutrients, and suppress weeds, there is a concern that cover crops (CCs) soil water use negatively impacts subsequent crops in water-limited environments. Cover crop management practices such as planting and termination timing may mitigate the detrimental impacts of CCs in semi-arid cropping systems. To determine the effects of CCs under water-limited environments, we evaluated the total CC biomass produced in the fall and spring, soil water content during the subsequent maize growing season, weed density and biomass, crop residue, and soil nutrients at the maize V6 development stage, and maize …


Contributions Of Individual Cover Crop Species To Rainfed Maize Production In Semi-Arid Cropping Systems, Alexandre T. Rosa, Cody Creech, Roger W. Elmore, Daran Rudnick, John L. Lindquist, Liberty Butts, Italo K. Pinho De Faria, Rodrigo Werle Sep 2021

Contributions Of Individual Cover Crop Species To Rainfed Maize Production In Semi-Arid Cropping Systems, Alexandre T. Rosa, Cody Creech, Roger W. Elmore, Daran Rudnick, John L. Lindquist, Liberty Butts, Italo K. Pinho De Faria, Rodrigo Werle

Department of Agronomy and Horticulture: Faculty Publications

Cover crop (CC) species selection can contribute to reducing soil penetration resistance (brassica species), improved soil nitrogen (N) cycling (legume species), and suppression of weeds (grass species). However, one of the main concerns about including CCs in water-limited environments is soil water use and the consequences to subsequent crops. To determine the effects of individual CC species under water-limited environments, we evaluated fall and spring CC biomass produced, and soil water and N content, penetration resistance, weed density and biomass during the maize growing season, and maize grain yield. The experiment was conducted under a winter wheat-maize-fallow rotation at two …


Tandem Duplicate Expression Patterns Are Conserved Between Maize Haplotypes Of The Α-Zein Gene Family, Preston Hurst, James C. Schnable, David R. Holding Aug 2021

Tandem Duplicate Expression Patterns Are Conserved Between Maize Haplotypes Of The Α-Zein Gene Family, Preston Hurst, James C. Schnable, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Tandem duplication gives rise to copy number variation and subsequent functional novelty among genes as well as diversity between individuals in a species. Functional novelty can result from either divergence in coding sequence or divergence in patterns of gene transcriptional regulation. Here, we investigate conservation and divergence of both gene sequence and gene regulation between the copies of the α-zein gene family in maize inbreds B73 and W22. We used RNA-seq data generated from developing, self-pollinated kernels at three developmental stages timed to coincide with early and peak zein expression. The reference genome annotations for B73 and W22 were modified …


Rhizosphere Microbiomes In A Historical Maize-Soybean Rotation System Respond To Host Species And Nitrogen Fertilization At The Genus And Subgenus Levels, Michael A. Meier, Martha G. Lopez-Guerrero, Ming Guo, Marty Schmer, Josh Herr, James Schnable, James R. Alfano, Jinliang Yang Jun 2021

Rhizosphere Microbiomes In A Historical Maize-Soybean Rotation System Respond To Host Species And Nitrogen Fertilization At The Genus And Subgenus Levels, Michael A. Meier, Martha G. Lopez-Guerrero, Ming Guo, Marty Schmer, Josh Herr, James Schnable, James R. Alfano, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

Root-associated microbes are key players in plant health, disease resistance, and nitrogen (N) use efficiency. It remains largely unclear how the interplay of biological and environmental factors affects rhizobiome dynamics in agricultural systems. In this study, we quantified the composition of rhizosphere and bulk soil microbial communities associated with maize (Zea mays L.) and soybean (Glycine max L.) in a long-term crop rotation study under conventional fertilization and low-N regimes. Over two growing seasons, we evaluated the effects of environmental conditions and several treatment factors on the abundance of rhizosphere- and soil-colonizing microbial taxa. Time of sampling, host plant species, …


72-H Diurnal Rna-Seq Analysis Of Fully Expanded Third Leaves From Maize, Sorghum, And Foxtail Millet At 3-H Resolution, Xianjun Lai, Claire Bendix, Yan Zhang, James Schnable, Frank G. Harmon Jan 2021

72-H Diurnal Rna-Seq Analysis Of Fully Expanded Third Leaves From Maize, Sorghum, And Foxtail Millet At 3-H Resolution, Xianjun Lai, Claire Bendix, Yan Zhang, James Schnable, Frank G. Harmon

Center for Plant Science Innovation: Faculty and Staff Publications

Objectives: The purpose of this data set is to capture the complete diurnal (i.e., daily) transcriptome of fully expanded third leaves from the C4 panacoid grasses sorghum (Sorghum bicolor), maize (Zea mays), and foxtail millet (Setaria italica) with RNA-seq transcriptome profiling. These data are the cornerstone of a larger project that examined the conservation and divergence of gene expression networks within these crop plants. This data set focuses on tem- poral changes in gene expression to identify the network architecture responsible for daily regulation of plant growth and metabolic activities. The power of this data set is fine temporal resolution …


High Plains Wheat Mosaic Virus: An Enigmatic Disease Of Wheat And Corn Causing The High Plains Disease, Satyanarayana Tatineni, Gary Hein Jan 2021

High Plains Wheat Mosaic Virus: An Enigmatic Disease Of Wheat And Corn Causing The High Plains Disease, Satyanarayana Tatineni, Gary Hein

Department of Entomology: Faculty Publications

Brief history: In 1993, severe mosaic and necrosis symptoms were observed on corn (maize) and wheat from several Great Plains states of the USA. Based on the geographical location of infections, the disease was named High Plains disease and the causal agent was tentatively named High Plains virus. Subsequently, researchers renamed this virus as maize red stripe virus and wheat mosaic virus to represent the host and symptom phenotype of the virus. After sequencing the genome of the pathogen, the causal agent of High Plains disease was officially named as High Plains wheat mosaic virus. Hence, High Plains virus, …


Leaf Angle Extractor: A High-Throughput Image Processing Framework For Leaf Angle Measurements In Maize And Sorghum, Sunil Kumar Kenchanmane Raju, Miles Adkins, Alex Enersen, Daniel Santana De Carvalho, Anthony J. Studer, Baskar Ganapathysubramanian, Patrick S. Schnable, James C. Schnable Jan 2020

Leaf Angle Extractor: A High-Throughput Image Processing Framework For Leaf Angle Measurements In Maize And Sorghum, Sunil Kumar Kenchanmane Raju, Miles Adkins, Alex Enersen, Daniel Santana De Carvalho, Anthony J. Studer, Baskar Ganapathysubramanian, Patrick S. Schnable, James C. Schnable

Center for Plant Science Innovation: Faculty and Staff Publications

PREMISE: Maize yields have significantly increased over the past half-century owing to advances in breeding and agronomic practices. Plants have been grown in increasingly higher densities due to changes in plant architecture resulting in plants with more upright leaves, which allows more efficient light interception for photosynthesis. Natural variation for leaf angle has been identified in maize and sorghum using multiple mapping populations. However, conventional phenotyping techniques for leaf angle are low throughput and labor intensive, and therefore hinder a mechanistic understanding of how the leaf angle of individual leaves changes over time in response to the environment.

METHODS …


Benchmarking Impact Of Nitrogen Inputs On Grain Yield And Environmental Performance Of Producer Fields In The Western Us Corn Belt, Fatima A.M. Tenorio, Eileen L. Mclellan, Alison J. Eagle, Kenneth G. Cassman, Daryl Andersen, Marie Krausnick, Russell Oaklund, John Thorburn, Patricio Grassini Jan 2020

Benchmarking Impact Of Nitrogen Inputs On Grain Yield And Environmental Performance Of Producer Fields In The Western Us Corn Belt, Fatima A.M. Tenorio, Eileen L. Mclellan, Alison J. Eagle, Kenneth G. Cassman, Daryl Andersen, Marie Krausnick, Russell Oaklund, John Thorburn, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Benchmarking crop yields against nitrogen (N) input levels can help provide opportunities to improve N ferti-lizer efficiency and reduce N losses on maize in the US Corn Belt by identifying fields most likely to benefit from improved N management practices. Here, we evaluated a large producer database that includes field-level data on yield and applied N inputs from 9280 irrigated and rainfed fields over a 7-year period (2009–2015) in Nebraska (USA). A spatial framework, based on technology extrapolation domains, was used to cluster each field into spatial units with similar climate and soil type that represent 1.3 million ha of …


Genome-Wide Identification And Analysis Of Heterotic Loci In Three Maize Hybrids, Hongjun Liu, Qin Wang, Mengjiao Chen, Yahui Ding, Xuerong Yang, Jie Liu, Xiaohan Li, Congcong Zhou, Qilin Tian, Yiqi Lu, Danlin Fan, Junpeng Shi, Lin Zhang, Congbin Kang, Mingfei Mingfei Sun, Fangyuan Li, Yujian Wu, Yongzhong Zhang, Baoshen Liu, Xiang Yu Zhao, Qi Feng, Jinliang Yang, Bin Han, Jinsheng Lai, Xian Sheng Zhang, Xuehui Huang Jan 2020

Genome-Wide Identification And Analysis Of Heterotic Loci In Three Maize Hybrids, Hongjun Liu, Qin Wang, Mengjiao Chen, Yahui Ding, Xuerong Yang, Jie Liu, Xiaohan Li, Congcong Zhou, Qilin Tian, Yiqi Lu, Danlin Fan, Junpeng Shi, Lin Zhang, Congbin Kang, Mingfei Mingfei Sun, Fangyuan Li, Yujian Wu, Yongzhong Zhang, Baoshen Liu, Xiang Yu Zhao, Qi Feng, Jinliang Yang, Bin Han, Jinsheng Lai, Xian Sheng Zhang, Xuehui Huang

Department of Agronomy and Horticulture: Faculty Publications

Heterosis, or hybrid vigour, is a predominant phenomenon in plant genetics, serving as the basis of crop hybrid breeding, but the causative loci and genes underlying heterosis remain unclear in many crops. Here, we present a large-scale genetic analysis using 5360 offsprings from three elite maize hybrids, which identifies 628 loci underlying 19 yield-related traits with relatively high mapping resolutions. Heterotic pattern investigations of the 628 loci show that numerous loci, mostly with complete–incomplete dominance (the major one) or overdominance effects (the secondary one) for heterozygous genotypes and nearly equal proportion of advantageous alleles from both parental lines, are the …


Benchmarking On-Farm Maize Nitrogen Balance In The Western U.S. Corn Belt, Fatima Amor Tenorio Dec 2019

Benchmarking On-Farm Maize Nitrogen Balance In The Western U.S. Corn Belt, Fatima Amor Tenorio

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

A nitrogen (N) balance, calculated as the difference between N inputs and grain-N removal, provides an estimate of the potential N losses. We used N balance with other N-related metrics (partial factor productivity for N inputs, and yield-scaled N balance), to benchmark maize yields in relation with N input use in the US Corn Belt. We first used experimental data on grain-N concentration (GNC) to assess variation in this parameter due to biophysical and management factors. Subsequently, we used N balance and N-related metrics to benchmark yields in relation with N inputs in irrigated and rainfed fields in Nebraska using …


New Approaches To Use Genomics, Field Traits, And High-Throughput Phenotyping For Gene Discovery In Maize (Zea Mays), Zhikai Liang Aug 2019

New Approaches To Use Genomics, Field Traits, And High-Throughput Phenotyping For Gene Discovery In Maize (Zea Mays), Zhikai Liang

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Maize is one of major crop species over the world. With lots of genetic resources and genomic tools, maize also serves as a model species to understand genetic diversity, facilitate the development of trait extraction algorithms and map candidate functional genes. Since the first version of widely used B73 reference genome was released, independent research groups in the maize community propagated seeds themselves for further research purposes. However, unexpected or occasional contamination may happen during this process. The first study in this thesis used public RNA-seq data of B73 from 27 research groups across three countries for calling single nucleotide …


Evaluation Of Foliar Insecticides For The Control Of Western Bean Cutworm In Field Corn, 2018, Katharine A. Swoboda-Bhattarai, Samantha R. Daniel, Julie A. Peterson Jan 2019

Evaluation Of Foliar Insecticides For The Control Of Western Bean Cutworm In Field Corn, 2018, Katharine A. Swoboda-Bhattarai, Samantha R. Daniel, Julie A. Peterson

West Central Research and Extension Center, North Platte

The objectives of this field trial were to evaluate the efficacy of single applications of foliar insecticides at preventing feeding damage by the western bean cutworm (WBC), an important pest of corn and dry beans that has undergone a rapid range expansion into the eastern Corn Belt during the last 18 yr. This study was conducted within the historic range of WBC, at the University of Nebraska-Lincoln’s Henry J. Stumpf International Wheat Center in Perkins County, NE (40.856851°N, −101.701335°W). An RCB design with a total of 16 treatments (including an untreated check) and four replications was used. Plots measured 20 …


Cover Crops Have Negligible Impact On Soil Water In Nebraska Maize–Soybean Rotation, J. Burdette Barker, Derek M. Heeren, Katja Koehler-Cole, Charles Shapiro, Humberto Blanco-Canqui, Roger W. Elmore, Christopher A. Proctor, Suat Irmak, Charles A. Francis, Tim M. Shaver, Ali T. Mohammed Aug 2018

Cover Crops Have Negligible Impact On Soil Water In Nebraska Maize–Soybean Rotation, J. Burdette Barker, Derek M. Heeren, Katja Koehler-Cole, Charles Shapiro, Humberto Blanco-Canqui, Roger W. Elmore, Christopher A. Proctor, Suat Irmak, Charles A. Francis, Tim M. Shaver, Ali T. Mohammed

Biological Systems Engineering: Papers and Publications

One perceived cost of integrating winter cover cropping in maize (Zea mays L.) and soybean [Glycine max (L.) Merr.] rotation systems is the potential negative impact on soil water storage available for primary crop production. The objective of this 3-year study was to evaluate the effects of winter cover crops on soil water storage and cover crop biomass production following no-till maize and soybean rotations. Locations were near Brule (west-central), Clay Center (south-central), Concord (northeast), and Mead (east-central), Nebraska, United States. Treatments included crop residue only (no cover crop) and a multi-species cover crop mix, both broadcast-seeded before …


Profile Of Genetically Modified Plants Authorized In Mexico, Mayra Teresa Garcia Ruiz, Aaron N. Knapp, Hernan Garcia Ruiz Jan 2018

Profile Of Genetically Modified Plants Authorized In Mexico, Mayra Teresa Garcia Ruiz, Aaron N. Knapp, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Mexico is a center of origin for several economically important plants including maize, cotton, and cocoa. Maize represents more than a food crop, has been declared a biological, cultural, agricultural and economic patrimony, and is linked to the national identity of Mexicans. In this review, we describe the historic and current use of genetically modified plants in Mexico and factors that contributed to the development of the biosafety regulation. We developed a database containing all permit applications received by the government to release genetically modified plants. A temporal and geographical analysis identified the plant species that have been authorized for …


Conventional And Hyperspectral Time-Series Imaging Of Maize Lines Widely Used In Field Trials, Zhikai Liang, Piyush Pandey, Vincent Stoerger, Yuhang Xu, Yumou Qiu, Yufeng Ge, James C. Schnable Jan 2018

Conventional And Hyperspectral Time-Series Imaging Of Maize Lines Widely Used In Field Trials, Zhikai Liang, Piyush Pandey, Vincent Stoerger, Yuhang Xu, Yumou Qiu, Yufeng Ge, James C. Schnable

Center for Plant Science Innovation: Faculty and Staff Publications

Background: Maize (Zea mays ssp. mays) is 1 of 3 crops, along with rice and wheat, responsible for more than one-half of all calories consumed around the world. Increasing the yield and stress tolerance of these crops is essential to meet the growing need for food. The cost and speed of plant phenotyping are currently the largest constraints on plant breeding efforts. Datasets linking new types of high-throughput phenotyping data collected from plants to the performance of the same genotypes under agronomic conditions across a wide range of environments are essential for developing new statistical approaches and computer …