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United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
- Keyword
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- Nitrogen (4)
- Corn (3)
- Risk assessment (3)
- Fusarium (2)
- Glyphosate (2)
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- Mineralization (2)
- Modeling (2)
- Runoff (2)
- Simulation (2)
- Sorghum (2)
- Watershed management (2)
- Wheat (2)
- 15N (1)
- ABA • Diphenyleneiodonium • Hydrogen peroxide • Nitric oxide • Reactive oxygen species • Seed germination • Switchgrass • Warm-season C4 grasses (1)
- AISA+ (1)
- Acclimation (1)
- Acetone (1)
- Acid hydrolysis (1)
- Adrenomedullin; Adrenomedullin binding protein; Mammary gland; Inflammation; Lactation (1)
- Agricultural watershed (1)
- Alkaline earth elements (1)
- Ammonia volatilization (1)
- Anatomy; Biomass; Cell-wall composition; GCMS; HPLC; Switchgrass; Soluble and wall-bound phenolics (1)
- Animal response (1)
- Apricot (1)
- Arabidopsis (1)
- Artificial neural networks (1)
- Aspergillus (1)
- Attractants (1)
- Avian influenza virus (1)
Articles 1 - 30 of 92
Full-Text Articles in Plant Sciences
Enhanced Uv Inactivation Of Adenoviruses Under Polychromatic Uv Lamps, Karl G. Linden, Jeanette Thurston, Raymond Schaefer, James P. Malley Jr.
Enhanced Uv Inactivation Of Adenoviruses Under Polychromatic Uv Lamps, Karl G. Linden, Jeanette Thurston, Raymond Schaefer, James P. Malley Jr.
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Adenovirus is recognized as the most UV-resistant waterborne pathogen of concern to public health microbiologists. The U.S. EPA has stipulated that a UV fluence (dose) of 186 mJ cm-2 is required for 4-log inactivation credit in water treatment. However, all adenovirus inactivation data to date published in the peer-reviewed literature have been based on UV disinfection experiments using UV irradiation at 253.7 nm produced from a conventional low-pressure UV source. The work reported here presents inactivation data for adenovirus based on polychromatic UV sources and details the significant enhancement in inactivation achieved using these polychromatic sources. When full-spectrum, medium-pressure …
A Physiological Basis For Controlling Leafy Spurge On Nebraska Rangeland, Rob B. Mitchell, Corey Moffet, Ron Sosebee
A Physiological Basis For Controlling Leafy Spurge On Nebraska Rangeland, Rob B. Mitchell, Corey Moffet, Ron Sosebee
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Applying herbicides at the appropriate physiological stage is an important consideration to effectively control leafy spurge.
Livestock Production System Management Responses To Thermal Challenges, J. A. Nienabar, G. L. Hahn
Livestock Production System Management Responses To Thermal Challenges, J. A. Nienabar, G. L. Hahn
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The adaptive capabilities of animals and livestock production systems have been emphasized in this report. Biometeorology has a key role in rational management to meet the challenges of thermal environments. While the focus is primarily on cattle in warm or hot climates, the importance of dynamic animal responses to environmental challenges applies to all species and climates. Methods used to mitigate environmental challenges focus on heat loss/heat production balance. Under cold stress, reduction of heat loss is the key. Under heat stress, reduction of heat load or increasing heat loss are the primary management tools, although heat-tolerant animals are also …
Characterization Of Polymeric Proteins From Vitreous And Floury Sorghum Endosperm, B. Ioerger, S. R. Bean, M. R. Tuinstra, Jeffrey F. Pedersen, J. Erpelding, K. M. Lee, T. J. Herrman
Characterization Of Polymeric Proteins From Vitreous And Floury Sorghum Endosperm, B. Ioerger, S. R. Bean, M. R. Tuinstra, Jeffrey F. Pedersen, J. Erpelding, K. M. Lee, T. J. Herrman
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Differences in protein content and composition between vitreous and floury endosperm were investigated using a number of different techniques. Differences in protein cross-linking between vitreous and floury endosperm were investigated using differential solubility, size exclusion chromatography (SEC), and analysis of sulfhydryl content and composition. Vitreous endosperm was found to have higher levels of total protein and kafirins, but floury endosperm had a higher proportion of γ-kafirins than the vitreous. Floury endosperm was found to have higher levels of SDS-soluble proteins than SDS-insoluble proteins extracted using sonication than vitreous endosperm. Conversely, vitreous endosperm had a greater proportion of the insoluble proteins. …
The Mycotoxin Threat To Food Safety, Ervin Balazs, James S. Schepers
The Mycotoxin Threat To Food Safety, Ervin Balazs, James S. Schepers
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
This workshop entitled “Mycotoxins from the Field to the Table” held in Omaha, Nebraska, USA from 29 November to 1 December, 2006 evolved from the OECD (Organization for Economic Co-operation and Development) Co-operative Research Program on “Biological Resource Management” that was initiated in 1979. It currently focuses on three specific areas, namely the natural resources challenge, sustainability in practice, and the food chain. This workshop was organized under the sustainability and food chain themes.
The Cooperative Research Program's two major activities are promotion of expert exchanges by supporting postdoctoral fellowship awards up to 26 weeks in an OECD host laboratory …
Source Tracking Of Microbial Intrusion In Water Systems Using Artificial Neural Networks, Minyoung Kim, Christopher Y. Choi, Charles P. Garba
Source Tracking Of Microbial Intrusion In Water Systems Using Artificial Neural Networks, Minyoung Kim, Christopher Y. Choi, Charles P. Garba
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
A ‘‘what-if’’ scenario where biological agents are accidentally or deliberately introduced into a water system was generated, and artificial neural network (ANN) models were applied to identify the pathogenic release location to isolate the contaminated area and minimize its hazards. The spatiotemporal distribution of Escherichia coli 15597 along the water system was employed to locate pollutants by inversely interpreting transport patterns of Escherichia coli using ANNs. Results showed that dispersion patterns of Escherichia coli were positively correlated to pH, turbidity, and conductivity (R2 = 0.90–0.96), and the ANN models successfully identified the source location of Escherichia coli introduced into …
Convergence Of Agriculture And Energy: Ii. Producing Cellulosic Biomass For Biofuels, Steven L. Fales, J. Richard Hess, Wallace Wilhelm, Don Erbach, William D. Provine, Kenneth P. Vogel, Todd A. Peterson, Edward C. A. Runge
Convergence Of Agriculture And Energy: Ii. Producing Cellulosic Biomass For Biofuels, Steven L. Fales, J. Richard Hess, Wallace Wilhelm, Don Erbach, William D. Provine, Kenneth P. Vogel, Todd A. Peterson, Edward C. A. Runge
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Global energy demand is increasing as known global petroleum supplies are decreas¬ing. Calls to supplement or replace the current fossil-based energy system with new, envi¬ronmentally and economically sustainable strategies continue to increase, especially in light of more expensive traditional energy sources. Various governmental agencies and working groups have set aggressive targets and timelines for decreasing fossil fuel consumption by substituting bio-based energy (Bush 2007; Foust et al. 2007; Perlack et al. 2005; Smith et al. 2004). The alignment and continuity of these goals is illustrated in Figure 1.
Current biofuel production in the United States relies primarily on corn grain …
Biofuel Feedstocks: The Risk Of Future Invasions, Joseph M. Ditomaso, Jacob N. Barney, Alison M. Fox
Biofuel Feedstocks: The Risk Of Future Invasions, Joseph M. Ditomaso, Jacob N. Barney, Alison M. Fox
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
In an effort to decrease greenhouse gas emissions, expand domestic energy production, and maintain economic growth, public and private investments are being used to pursue dedicated feedstock crops for biofuel production. Unlike food crops grown for grain-based ethanol (e.g., corn), which require high inputs of fertilizers and pesticides and typically are grown on prime agricultural land, proposed lignocellulose-based energy crops (e.g., switchgrass) typically have a neutral or negative carbon budget, require relatively few economic or environmental inputs, and can be cultivated on marginal, lower-productivity land. Thus, a rapidly growing industry related to crop selection, cultivar improvement, and conversion technologies is …
K12-Biotinylated Histone H4 Marks Heterochromatin In Human Lymphoblastoma Cells, Gabriela Camporeale, Anna M. Oommen, Jacob B. Griffin, Gautam Sarath, Janos Zempleni
K12-Biotinylated Histone H4 Marks Heterochromatin In Human Lymphoblastoma Cells, Gabriela Camporeale, Anna M. Oommen, Jacob B. Griffin, Gautam Sarath, Janos Zempleni
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Covalent modifications of histones play crucial roles in chromatin structure and genomic stability. Recently, we reported a novel modification of histones: biotinylation of lysine residues. Here we provide evidence that K12-biotinylated histone H4 (K12Bio H4) maps specifically to both heterochromatin (alpha satellite repeats in pericentromeric regions) and transcriptionally repressed chromatin (γ-G globin and interleukin-2) in human lymphoblastoma cells. The abundance of K12Bio H4 in these regions was similar to that of K9-dimethylated histone H3, a known marker for heterochromatin. Likewise, K8-biotinylated histone H4 (K8Bio H4) mapped to heterochromatin, but the relative enrichment was smaller compared with K12Bio H4. Stimulation of …
Engineering, Nutrient Removal, And Feedstock Conversion Evaluations Of Four Corn Stover Harvest Scenarios, Reed L. Hoskinson, Douglas L. Karlen, Stuart J. Birrell, Corey W. Radtke, Wally Wilhelm
Engineering, Nutrient Removal, And Feedstock Conversion Evaluations Of Four Corn Stover Harvest Scenarios, Reed L. Hoskinson, Douglas L. Karlen, Stuart J. Birrell, Corey W. Radtke, Wally Wilhelm
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Crop residue has been identified as a near-term source of biomass for renewable fuel, heat, power, chemicals and other bio-materials. A prototype one-pass harvest system was used to collect residue samples from a corn (Zea mays L.) field near Ames, IA. Four harvest scenarios (low cut, high-cut top, high-cut bottom, and normal cut) were evaluated and are expressed as collected stover harvest indices (CSHI). High-cut top and high-cut bottom samples were obtained from the same plot in separate operations. Chemical composition, dilute acid pretreatment response, ethanol conversion yield and efficiency, and thermochemical conversion for each scenario were determined. Mean …
Source Tracking Of Microbial Intrusion In Water Systems Using Artificial Neural Networks, Minyoung Kim, Christopher Y. Choi, Charles P. Gerba
Source Tracking Of Microbial Intrusion In Water Systems Using Artificial Neural Networks, Minyoung Kim, Christopher Y. Choi, Charles P. Gerba
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
A ‘‘what-if’’ scenario where biological agents are accidentally or deliberately introduced into a water system was generated, and artificial neural network (ANN) models were applied to identify the pathogenic release location to isolate the contaminated area and minimize its hazards. The spatiotemporal distribution of Escherichia coli 15597 along the water system was employed to locate pollutants by inversely interpreting transport patterns of E. coli using ANNs. Results showed that dispersion patterns of E. coli were positively correlated to pH, turbidity, and conductivity (R2 = 0.90–0.96), and the ANN models successfully identified the source location of E. coli introduced into …
Comparison Of Laboratory Methods And An In Situ Method For Estimating Nitrogen Mineralization In An Irrigated Silt-Loam Soil, Brian J. Wienhold
Comparison Of Laboratory Methods And An In Situ Method For Estimating Nitrogen Mineralization In An Irrigated Silt-Loam Soil, Brian J. Wienhold
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Nitrogen (N) mineralization makes a considerable contribution to crop-available N and is difficult to estimate. Reliable methods for measuring N mineralization are needed to produce data sets for developing N-mineralization models, as a component in fertilizer recommendation algorithms, and to assess the effect of management practices on N mineralization. Numerous methods are available for estimating N mineralization. Laboratory methods are relatively easy but may not reflect conditions in the field, and field methods are usually labor-intensive. A study was conducted to compare N-mineralization estimates using anaerobic and aerobic laboratory methods and an in situ field method for the 0- to …
Usda-Ars Lincoln Location Contact Information
Usda-Ars Lincoln Location Contact Information
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Grain, Forage, & Bioenergy Research Unit
Mid-West Livestock Research Unit
Soil & Water Conservation Research Unit
Employee Listing
The mission of the USDA / ARS programs at Lincoln, Nebraska is to improve the productivity, stability of production, sustainability and profitability of crop and livestock production systems in the Great Plains
The Lincoln Location is a part of the Northern Plains Area (NPA) of the Agricultural Research Service (ARS), a Research, Education and Economics (REE) agency, and the main in-house research arm of the United States Department of Agriculture (USDA)
Internode Structure And Cell Wall Composition In Maturing Tillers Of Switchgrass (Panicum Virgatum. L), Gautam Sarath, Lisa M. Baird, Kenneth P. Vogel, Robert B. Mitchell
Internode Structure And Cell Wall Composition In Maturing Tillers Of Switchgrass (Panicum Virgatum. L), Gautam Sarath, Lisa M. Baird, Kenneth P. Vogel, Robert B. Mitchell
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
This work examined cell composition gradients in maturing tillers of switchgrass (Panicum virgatuln L. ) with the aim of developing baseline information on this important forage and biomass crop. Flowering tillers were collected from plants raised from seeds in a greenhouse and field, harvested at soil level and separated into internodes beginning with the node subtending the peduncle. Internodes were analyzed using microscopy, by fiber digestion, high-performance liquid chromatography and by gas chromatography-mass spectrometry to obtain anatomical and compositional data. Microscopy demonstrated the development and maturation of cortical fibers which eventually became confluent with the fiber sheath surrounding vascular …
Cloning And Expression Of An Atrazine Inducible Cytochrome P450, Cyp4g33, From Chironomus Tentans (Diptera: Chironomidae), Diana K. Londono, Herbert A.A. Siqueira, Haichuan Wang, Gautam Sarath, Michael J. Lydy, Blair D. Siegfried
Cloning And Expression Of An Atrazine Inducible Cytochrome P450, Cyp4g33, From Chironomus Tentans (Diptera: Chironomidae), Diana K. Londono, Herbert A.A. Siqueira, Haichuan Wang, Gautam Sarath, Michael J. Lydy, Blair D. Siegfried
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Previous studies performed in our laboratory have measured the effect of atrazine exposure on cytochrome P450-dependent monooxygenase activity and have found increased activity in midge larvae (Chironomus tentans) as a result of atrazine exposure (1– 10 ppm). Here we report the cloning and expression of a specific C. tentans CYP4 gene that is responsive to atrazine induction with an open reading frame of 1678 bp which encodes a putative protein of 559 amino acid residues. Alignments of deduced amino acid sequences with other insect P450 genes and phylogenetic analysis indicated a high degree of similarity to other insect …
Comparative Genomics Reveals Functional Transcriptional Control Sequences In The Prop1 Gene, Robert D. Ward, Minchul Cho, S. A. Camper, Shannon W. Davis, Lori T. Raetzman, Timothy P.L. Smith, Constance Esposito, Robert H. Lyons, Jan-Fang Cheng, Edward M. Rubin, Simon J. Rhodes
Comparative Genomics Reveals Functional Transcriptional Control Sequences In The Prop1 Gene, Robert D. Ward, Minchul Cho, S. A. Camper, Shannon W. Davis, Lori T. Raetzman, Timothy P.L. Smith, Constance Esposito, Robert H. Lyons, Jan-Fang Cheng, Edward M. Rubin, Simon J. Rhodes
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Mutations in PROP1 are a common genetic cause of multiple pituitary hormone deficiency (MPHD). We used a comparative genomics approach to predict the transcriptional regulatory domains of Prop1 and tested them in cell culture and mice. A BAC transgene containing Prop1 completely rescues the Prop1 mutant phenotype, demonstrating that the regulatory elements necessary for proper Prop1 transcription are contained within the BAC. We generated DNA sequences from the PROP1 genes in lemur, pig, and five different primate species. Comparison of these with available human and mouse PROP1 sequences identified three putative regulatory sequences that are highly conserved. These are located …
Tillage And Rotation Effect On Corn–Soybean Energy Balances In Eastern Nebraska, G.-W. Rathke, Brian Wienhold, Wallace Wilhelm, W. Diepenbrock
Tillage And Rotation Effect On Corn–Soybean Energy Balances In Eastern Nebraska, G.-W. Rathke, Brian Wienhold, Wallace Wilhelm, W. Diepenbrock
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Data from a field experiment conducted in eastern Nebraska over 16 years (1986–2001) were used to determine the energy balance of corn (Zea mays L.) and soybean (Glycine max L.) as affected by tillage treatments and rotation. Tillage treatments included chisel plow, tandem disk, moldboard plow, ridge-tillage, no-till and subsoil tillage. Crop sequences were continuous corn, continuous soybean, corn in a corn–soybean rotation and soybean in a soybean–corn rotation. The energy balance was assessed by comparing the parameters: energy gain (net energy output), energy intensity (energy input per unit grain equivalent, GE) and output/input ratio. Changes in plant …
What We Do Know About Rice (Oryza Sativa) Hemoglobins, Veronica Lira-Ruan, Emily H. Ross, Mark S. Hargrove, Gautam Sarath, Raul Arredondo-Peter, Robert V. Klucas
What We Do Know About Rice (Oryza Sativa) Hemoglobins, Veronica Lira-Ruan, Emily H. Ross, Mark S. Hargrove, Gautam Sarath, Raul Arredondo-Peter, Robert V. Klucas
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Nonsymbiotic hemoglobins (nsHb) are proteins widely distributed in land plants, however their function in plant organs is still not known. Rice nsHbs have been characterized in detail and represent a model to study plant nsHbs. Rice recombinant Hb1 (rHb1) has a very high affinity for O2 (1,800 μM-1 s-1), because the dissociation constant is extremely low (koff = 0.038 s-1). X-ray analysis and site-directed mutagenesis showed that the low dissociation constant of rHb1 is partially due to the stabilization of bound O2 by distal His. This characteristic suggests that the function of …
An Avidin-Based Assay For Histone Debiotinylase Activity In Human Cell Nuclei, Yap Ching Chew, Gautam Sarath, Janos Zempleni
An Avidin-Based Assay For Histone Debiotinylase Activity In Human Cell Nuclei, Yap Ching Chew, Gautam Sarath, Janos Zempleni
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The covalent binding of biotin to histones participates in heterochromatin formation, cell cycle progression and cellular response to DNA breaks. Biotinylation of histones appears to be a reversible process, but the identity of enzymes that remove biotin marks is largely unknown. Our long-term goal is to identify histone debiotinylases in human cells. Here we developed an avidin-based plate assay to quantify histone debiotinylase activities in nuclear extracts. This assay is an essential first step in purifying and identifying histone debiotinylases from human cells. Using this assay, we demonstrated that debiotinylation of histones depends on temperature and pH, consistent with enzyme …
Modeling Beaver Dam Effects On Ecohydraulics And Sedimentation In An Agricultural Watershed, Mary Carla Mccullough, Dean E. Eisenhauer, Michael G. Dosskey, David M. Admiraal
Modeling Beaver Dam Effects On Ecohydraulics And Sedimentation In An Agricultural Watershed, Mary Carla Mccullough, Dean E. Eisenhauer, Michael G. Dosskey, David M. Admiraal
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Populations of North American beaver (Castor canadensis) have increased in recent decades throughout the agricultural Midwestern U.S., leading to an increase in the frequency of beaver dams in small streams. The impact of beaver dams on channel structure in this region is not known. Our field observations indicate that beaver dams are too dynamic and their affects on channel structure occur over longer time frames than is practical to study with field measurements. Modeling is therefore needed to determine if beaver dams will help stabilize and aggrade incised streams. The objective of this paper is to determine how …
Effects Of Forage Management On Pasture Productivity And Phosphorus Content, M. M. Haan, J. R. Russell, J. L. Kovar, W. J. Powers, J. L. Benning
Effects Of Forage Management On Pasture Productivity And Phosphorus Content, M. M. Haan, J. R. Russell, J. L. Kovar, W. J. Powers, J. L. Benning
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The objectives of the current study were to determine the amounts of above- and below-ground plant biomass production, P uptake by forage, and P concentration of cool-season grass forage as influenced by management and season. Five forage management treatments were evaluated over 3 years in smooth bromegrass (Bromus inermis Leyss) pastures. Management practices were: ungrazed (U), hay harvest/fall stockpile grazing (HS), rotational stocking to residual sward heights of 10 (10R) or 5 (5R) cm, and continuous stocking to maintain sward height at 5 cm (5C). Forage samples were hand-clipped within and outside grazing exclosures monthly from April through November …
Molecular Serotyping Of Escherichia Coli O111:H8, Lisa M. Durso, James L. Bono, James E. Keen
Molecular Serotyping Of Escherichia Coli O111:H8, Lisa M. Durso, James L. Bono, James E. Keen
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
AccurateE. coli serotyping is critical for pathogen diagnosis and surveillance of non-O157 Shiga-toxigenic strains, however few laboratories have this capacity. The molecular serotyping protocol described in this paper targets the somatic and flagellar antigens of E. coli O111:H8 used in traditional serotyping, and can be performed routinely in the laboratory.
An Algorithm For Corn Nitrogen Recommendations Using A Chlorophyll Meter Based Sufficiency Index, Gary E. Varvel, Wally Wilhelm, J. F. Shanahan, James S. Schepers
An Algorithm For Corn Nitrogen Recommendations Using A Chlorophyll Meter Based Sufficiency Index, Gary E. Varvel, Wally Wilhelm, J. F. Shanahan, James S. Schepers
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Nitrogen fertilizer continues to be the major input influencing corn (Zea mays L.) yield in the Midwest. Improved N recommendations should result in greater N use efficiency and producer profit while reducing surface and groundwater contamination. This study was conducted to develop a plant-based technique to detect and correct N deficiencies during the season. Chlorophyll meter readings and grain yield were collected from corn in irrigated monoculture corn and soybean [Glycine max (L.) Merr.]–corn cropping systems with four hybrids and five N fertilizer application rates in the Platte Valley near Shelton, NE. Normalized chlorophyll meter readings (sufficiency index, …
An Algorithm For Corn Nitrogen Recommendations Using A Chlorophyll Meter Based Sufficiency Index, G. E. Varvel, W. W. Wilhelm, J. F. Shanahan, J. S. Schepers
An Algorithm For Corn Nitrogen Recommendations Using A Chlorophyll Meter Based Sufficiency Index, G. E. Varvel, W. W. Wilhelm, J. F. Shanahan, J. S. Schepers
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Nitrogen fertilizer continues to be the major input influencing corn (Zea mays L.) yield in the Midwest. Improved N recommendations should result in greater N use efficiency and producer profit while reducing surface and groundwater contamination. This study was conducted to develop a plant-based technique to detect and correct N deficiencies during the season. Chlorophyll meter readings and grain yield were collected from corn in irrigated monoculture corn and soybean [Glycine max (L.) Merr.]–corn cropping systems with four hybrids and five N fertilizer application rates in the Platte Valley near Shelton, NE. Normalized chlorophyll meter readings (sufficiency index, …
Loss Of Alkaline Earth Elements By Runoff From Agricultural Watersheds, M. A. Elrashidi, D. Hammer, M. D. Mays, C. A. Seybold, S. D. Peaslee
Loss Of Alkaline Earth Elements By Runoff From Agricultural Watersheds, M. A. Elrashidi, D. Hammer, M. D. Mays, C. A. Seybold, S. D. Peaslee
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The loss of agricultural chemicals in runoff from agricultural land is a major cause of poor surface water quality in the United State. Scientists (Natural Resources Conservation Service) developed a technique using climatic, hydrologic, and soil survey information to estimate the impact of agricultural watersheds on natural water resources. The objective of this study was to apply this technique on the Wagon Train (WT) watershed to predict loss of alkaline earth elements (calcium [Ca], magnesium [Mg], barium [Ba], and strontium [Sr]) by runoff from soils and to estimate elements loading into WT reservoir. The predicted losses of Ca, Mg, Ba, …
Reactive Oxygen Species, Aba And Nitric Oxide Interactions On The Germination Of Warm-Season C4-Grasses, Gautam Sarath, Guichuan Hou, Lisa M. Baird, Robert B. Mitchell
Reactive Oxygen Species, Aba And Nitric Oxide Interactions On The Germination Of Warm-Season C4-Grasses, Gautam Sarath, Guichuan Hou, Lisa M. Baird, Robert B. Mitchell
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Hydrogen peroxide (H2O2) as a source of reactive oxygen species (ROS) significantly stimulated germination of switchgrass (Panicum virgatum L.) seeds with an optimal concentration of 20 mM at both 25 and 35°C. For non-dormant switchgrass seeds exhibiting different levels of germination, treatment with H2O2 resulted in rapid germination (days) of all germinable seeds as compared to seeds placed on water. Exposure to 20 mM H2O2 elicited simultaneous growth of the root and shoot system, resulting in more uniform seedling development. Seeds of big bluestem (Andropogon gerardii Vitman) and …
Soil Carbon Dynamics During A Long-Term Incubation Study Involving 13C And 14C Measurements, Ronald F. Follett, Eldor A. Paul, Elizabeth G. Pruessner
Soil Carbon Dynamics During A Long-Term Incubation Study Involving 13C And 14C Measurements, Ronald F. Follett, Eldor A. Paul, Elizabeth G. Pruessner
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Soil organic matter is the earth's largest terrestrial reservoir of carbon (C). Thus, it serves as a major control on atmospheric carbon dioxide (CO2) levels. To better understand these controls, decreases in soil organic C (SOC), soil microbial biomass (SMB) C, and the role of SMB as a source of mineralizable C were measured during a long-term incubation (853 days) without added substrate. The 2 soils used were a Weld loam (fine montmorillonitic, mesic, Aridic Paleustoll) from near Akron, Colorado, and a Duroc loam (fine silty, mixed mesic Pachic Haplustoll) from near Sidney, Nebraska. The Akron soil was …
Greater Diversity Of Shiga Toxin-Encoding Bacteriophage Insertion Sites Among Escherichia Coli O157:H7 Isolates From Cattle Than In Those From Humans, Thomas E. Besser, Nurmohammad Shaikh, Nicholas J. Holt, Phillip I. Tarr, Michael E. Konkel, Preeti Malik-Kale, Coilin W. Walsh, Thomas S. Whittam, James L. Bono
Greater Diversity Of Shiga Toxin-Encoding Bacteriophage Insertion Sites Among Escherichia Coli O157:H7 Isolates From Cattle Than In Those From Humans, Thomas E. Besser, Nurmohammad Shaikh, Nicholas J. Holt, Phillip I. Tarr, Michael E. Konkel, Preeti Malik-Kale, Coilin W. Walsh, Thomas S. Whittam, James L. Bono
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
E. coli O157:H7, a zoonotic human pathogen for which domestic cattle are a reservoir host, produces a Shiga toxin(s) (Stx) encoded by bacteriophages. Chromosomal insertion sites of these bacteriophages define three principal genotypes (clusters 1 to 3) among clinical isolates of E. coliO157:H7. Stx-encoding bacteriophage insertion site genotypes of 282 clinical and 80 bovine isolates were evaluated. A total of 268 (95.0%) of the clinical isolates, but only 41 (51.3%) of the bovine isolates, belonged to cluster 1, 2, or 3 (P < 0.001). Thirteen additional genotypes were identified in isolates from both cattle and humans (four genotypes), from only cattle (seven genotypes), or from only humans (two genotypes). Two other markers previously associated with isolates from cattle or with clinical isolates showed similar associations with genotype groups within bovine isolates; the tir allele sp-1 and the Q933W allele were under- and overrepresented, respectively, among cluster 1 to …
Occurrence Of The Waxy Alleles Wxa And Wxb In Waxy Sorghum Plant Introductions And Their Effect On Starch Thermal Properties, Jeffrey F. Pedersen, Robert A. Graybosch, D. L. Funnell
Occurrence Of The Waxy Alleles Wxa And Wxb In Waxy Sorghum Plant Introductions And Their Effect On Starch Thermal Properties, Jeffrey F. Pedersen, Robert A. Graybosch, D. L. Funnell
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The existence of two waxy alleles, wxa associated with no detectable granule bound starch synthase (GBSS) and wxb associated with apparently inactive GBSS, was recently reported in sorghum [Sorghum bicolor (L.) Moench]. In this paper, the occurrence of the wxa and wxb alleles in the USDA-ARS photoperiod-insensitive sorghum collection was determined, and the effects of the wxa and wxballeles on thermal properties of sorghum starch (gelatinization temperatures and energy requirements) measured by differential scanning calorimetry. Of the 51 purported waxy accessions examined, 14 tested positive for presence of amylose by iodine staining and …
Classification Of Dry-Milled Maize Grit Yield Groups Using Quadratic Discriminant Analysis And Decision Tree Algorithm, Kyung-Min Lee, Timothy J. Herrman, Scott R. Bean, David S. Jackson, Jane Lingenfelser
Classification Of Dry-Milled Maize Grit Yield Groups Using Quadratic Discriminant Analysis And Decision Tree Algorithm, Kyung-Min Lee, Timothy J. Herrman, Scott R. Bean, David S. Jackson, Jane Lingenfelser
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
A genetically and environmentally diverse collection of maize (Zea maize L.) samples was evaluated for physical properties and grit yield to help develop a standard set of criteria to identify grain best suited for dry milling. Application of principal component analysis (PCA) reduced a set of approximately 500 samples collected from six states to 154 maize hybrids. Selected maize hybrids were placed into seven groups according to their dry-milled grit yields. Regression analysis explained only 50% of the variability in dry-milling grit yield. Patterns of differences in the physical properties for the seven grit yield groups implied that the …