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Full-Text Articles in Plant Sciences
Transgenic Control Of Aflatoxin Contamination In Maize Through Host-Induced Gene Silencing Targeting Aspergillus Flavus Genes Encoding Polygalacturonase (P2c) And Versicolorin Dehydrogenase (Aflm), Yenjit Raruang
LSU Doctoral Dissertations
Maize (Zea mays L.) is susceptible to Aspergillus flavus infection and subsequent contamination with aflatoxins, the most potent naturally produced carcinogenic secondary metabolites. Here, the A. flavus gene aflM encoding the versicolorin dehydrogenase in aflatoxin biosynthesis and the p2c gene encoding the polygalacturonase that is involved in infection were selected as targets for suppression through host induced gene silencing (HIGS). A HIGS vector targeting these genes was constructed and introduced into immature B104 maize embryos. Thirteen out of fifteen p2c transformation events and six out of seven aflM events were confirmed positive by PCR. Kernels containing the p2c gene …
Validation Of Candidate Loci For Maize Regrowability And Selection Of Near Isogenic Lines For The Loci, Tajbir Raihan
Validation Of Candidate Loci For Maize Regrowability And Selection Of Near Isogenic Lines For The Loci, Tajbir Raihan
Electronic Theses and Dissertations
Developing perennial grain crops is an effective way for sustainable agriculture and it can be a tool for the betterment of the overall ecosystem. To understand how perenniality works in perennial species, we made a cross between Zea diploperennis and maize (Z. mays) inbred line B73. F1 hybrids produced from this cross were selfed to get the F2 population and later the F2 population underwent Genotyping-By- Sequencing (GBS) analysis. Based on the candidate loci identified on chromosome 2 and chromosome 7 from GBS, SNP-specific markers were developed from the candidate locus interval and used to genotype the F2 …