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Full-Text Articles in Plant Sciences
Identification And Characterization Of An Efficient Acyl-Coa:Diacylglycerol Acyltransferase 1 (Dgat1) Gene From The Microalga Chlorella Ellipsoidea, Xuejie Guo, Chengming Fan, Yuhong Chen, Jingqiao Wang, Weibo Yin, Richard R. C. Wang, Zanmin Hu
Identification And Characterization Of An Efficient Acyl-Coa:Diacylglycerol Acyltransferase 1 (Dgat1) Gene From The Microalga Chlorella Ellipsoidea, Xuejie Guo, Chengming Fan, Yuhong Chen, Jingqiao Wang, Weibo Yin, Richard R. C. Wang, Zanmin Hu
Forage and Range Research Laboratory Publications
Background: Oil in the form of triacylglycerols (TAGs) is quantitatively the most important storage form of energy for eukaryotic cells. Diacylglycerol acyltransferase (DGAT) is considered the rate-limiting enzyme for TAG accumulation. Chlorella, a unicellular eukaryotic green alga, has attracted much attention as a potential feedstock for renewable energy production. However, the function of DGAT1 in Chlorella has not been reported.
Results: A full-length cDNA encoding a putative diacylglycerol acyltransferase 1 (DGAT1, EC 2.3.1.20) was obtained from Chlorella ellipsoidea. The 2,142 bp open reading frame of this cDNA, designated CeDGAT1, encodes a protein of 713 amino acids showing …