Plant Sciences Commons^™

Integrated Management Strategies For Phytophthora Sojae Combining Host Resistance And Seed Treatments, A. E. Dorrance, A. E. Robertson, S. Cianzo, L. J. Giesler, C. R. Grau, M. A. Draper, A. U. Tenuta, T. R. Anderson

Department of Plant Pathology: Faculty Publications

Phytophthora sojae has re-emerged as a serious soybean pathogen in the past decade. This may be due in part to changes in resistance levels in current cultivars, adoption of P. sojae populations to deployed Rps genes, and highly favorable environments in the past decade. This multilocation study evaluated the effect of seed treatments on the incidence and severity of Phytophthora root and stem rot on soybeans with different combinations of Rps genes and levels of partial resistance. The efficacy of the seed treatments was highly variable across locations. Seed treatments (metalaxyl and mefenoxam) provided protection and increased yields across cultivars …

A High-Oleic-Acid And Low-Palmitic-Acid Soybean: Agronomic Performance And Evaluation As A Feedstock For Biodiesel, George L. Graef, Bradley J. Lavallee, Patrick Tenopir, Mustafa Tat, Bruce Schweiger, Anthony J. Kinney, Jon H. Van Gerpen, Thomas E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Phenotypic characterization of soybean event 335-13, which possesses oil with an increased oleic acid content (> 85%) and reduced palmitic acid content (< 5%), was conducted across multiple environments during 2004 and 2005. Under these conditions, the stability of the novel fatty acid profile of the oil was not influenced by environment. Importantly, the novel soybean event 335-13 was not compromised in yield in both irrigated and non-irrigated production schemes. Moreover, seed characteristics, including total oil and protein, as well as amino acid profile, were not altered as a result of the large shift in the fatty acid profile. The novel oil trait was inherited in a simple Mendelian fashion. The event 335-13 was also evaluated as a feedstock for biodiesel. Extruded oil from event 335-13 produced a biodiesel with improved cold flow and enhanced oxidative stability, two critical fuel parameters that can limit the utility of this renewable transportation fuel.

Chlorovirus-Mediated Membrane Depolarization Of Chlorella Alters Secondary Active Transport Of Solutes, Irina V. Agarkova, David Dunigan, James R. Gurnon, Timo Greiner, Julia Barres, Gerhard Thiel, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Paramecium bursaria chlorella virus 1 (PBCV-1) is the prototype of a family of large, double-stranded DNA, plaque-forming viruses that infect certain eukaryotic chlorella-like green algae from the genus Chlorovirus. PBCV-1 infection results in rapid host membrane depolarization and potassium ion release. One interesting feature of certain chloroviruses is that they code for functional potassium ion-selective channel proteins (Kcv) that are considered responsible for the host membrane depolarization and, as a consequence, the efflux of potassium ions. This report examines the relationship between cellular depolarization and solute uptake. Annotation of the virus host Chlorella strain NC64A genome revealed 482 putative …

Shaping The Cross Timbers With Fire And Grazing, John A. Guretzky

Department of Agronomy and Horticulture: Faculty Publications

Recently, our agricultural research team has been asked to provide talks during tours of our Oswalt Road Ranch. The Oswalt Road Ranch is a 4,992-acre property in Love County, Okla., willed to us by D. Joyce Coffey. The Noble Foundation took full control of the property in 2000. The ranch is a valuable piece of property because of its unique natural beauty and potential to support livestock production and wildlife habitat. On the tours, we have been showing off our new state-of-the art livestock handling facilities and providing information about results from recent research on by-product feeds, the Noble Foundation …

Efficient Utilization Of Water And Nitrogen Resources For Grain Sorghum Under Rainfed Conditions, Akwasi A. Abunyewa

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Grain sorghum (Sorghum bicolor L. Moench) is the fifth world leading cereal after maize, wheat, rice and barley. The crop can yield reasonably well under adverse conditions of low soil water and high temperature. A three year field study was conducted in a transect across Nebraska where annual mean precipitation ranges from 300 to 900 mm yr^-1 to evaluate management practices to optimize yield potential under water limiting conditions. Loss in grain yield due to planting configurations ranged from 20 to 30% with skip-row configurations compared to conventional planting configuration (s0) at the site with greatest precipitation. At …

Apoyo Del Intsormil Incrementa La Produccion Y Productividad Del Cultivo De Sorgo En America Central, Intsormil

INTSORMIL Impacts and Bulletins

El INSORMIL ha apoyado, las actividades de investigación y transferencia de tecnología de sorgo desde 1981, con énfasis en Nicaragua y El Salvador pero en los ultimos años se viene expandiendo a otros países de America Central. Estadísticas del Ministerio de Agricultura y Ganadería de El Salvador, indican un signifi cativo incremento en la producción nacional de grano de sorgo en la misma superfi cie sembrada desde hace 9 años, debido principalmente al incremento en el rendimiento por unidad de superfi cie, tal como se presenta en el gráfi co. En este gráfi co puede verse que desde 1999 al …

Intsormil Support Contributes To Increased Sorghum Yields In Central America, Intsormil

INTSORMIL Impacts and Bulletins

INTSORMIL has supported sorghum research and technology transfer activities in Central America since 1981. Emphasis has been on El Salvador and Nicaragua but now the program is expanding to other Central American countries. Statistics provided by the Ministry of Agriculture indicate a significant advance in sorghum production in El Salvador. As shown in the graph below surface area in ha sown to sorghum during the period 1999-2009 did not increase (about 105,000 ha). However, during that same period grain production increased from 140,000 MT to 186,000 MT, an increase of 33%. This is due to the dramatic yield increase per …

Altos Costos Del Trigo: El Caso De La Harina De Sorgo En La Industria De La Panifi Cacion De El Salvador, Intsormil

INTSORMIL Impacts and Bulletins

El programa de tecnología de alimentos del CENTA, con el apoyo del INTSORMIL ha estado desarrollando recetas de pan a base de harina de sorgo y probándolas en pequeñas y medianas panaderías de El Salvador por muchos años. Ahora con los precios de harina de trigo a $0.57 por libra, los panaderos de El Salvador han demostrado un creciente interés en el uso del sorgo a un costo de $0.32 por libra como un sustituto parcial de la harina de trigo en sus productos. Los tecnólogos de alimentos del CENTA han estado promoviendo el uso y consumo de harina de …

First Report Of Soybean Rust Caused By Phakopsora Pachyrhizi In Nebraska, S. R. Watson, L. J. Giesler, A. D. Ziems, T. E. Brovont

Department of Plant Pathology: Faculty Publications

Soybean rust caused by Phakopsora pachyrhizi Sydow was first observed in the continental United States in Louisiana in November 2004 (2). As part of the national soybean rust monitoring effort, samples were collected on 3 October 2007 during the scouting of fields with green leaves in southeastern Nebraska. After incubation at room temperature for 24 h, uredinea and urediniospores were observed with microscopic examination. Urediniospores were obovoid, hyaline to pale brown, and measured 20 to 30 × 18 to 20 μm.

Alternative Cereal Processing Technologies [Conference Program And Proceedings] (Lobatse, Botswana, November 4-6, 2008), Martin Kebakile, Kemelo Ookeditse

INTSORMIL Presentations

Objectives of the workshop:

To review the current status of local cereals production and processing explore opportunities for growing the cereal industry.

To promote diversification of cereal processing by sensitizing all players and stakeholders in the cereals industry about readily available alternative cereals processing technologies which have commercial potential.

To promote and strengthen collaboration between local and international researchers, as well as fostering strategic partnerships between researchers and users of technologies to develop customer tailored processing technologies.

Epithelial And Mesenchymal Cells In The Bovine Colonic Mucosa Differ In Their Responsiveness To Escherichia Coli Shiga Toxin 1, Ivonne Stamm, Melanie Mohr, Philip S. Bridger, Elmar Schröpfer, Matthias König, William C. Stoffregen, Evelyn A. Dean-Nystrom, Georg Baljer, Christian Menge

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Bovine colonic crypt cells express CD77 molecules that potentially act as receptors for Shiga toxins (Stx). The implication of this finding for the intestinal colonization of cattle by human pathogenic Stx-producing Escherichia coli (STEC) remains undefined. We used flow cytometric and real-time PCR analyses of primary cultures of colonic crypt cells to evaluate cell viability, CD77 expression, and gene transcription in the presence and absence of purified Stx1. A subset of cultured epithelial cells had Stx receptors which were located mainly intracellularly, with a perinuclear distribution, and were resistant to Stx1-induced apoptosis and Stx1 effects on chemokine expression patterns. In …

Usaid/Mali Awards $5 M Associate Cooperative Agreement To Intsormil For Food Insecurity Mitigation In Mali, West Africa, Kimberly Christiansen

INTSORMIL Impacts and Bulletins

Dr. John Yohe, Program Director of the “Sorghum, Millet and other Grains Collaborative Research Support Program” (INTSORMIL) announces receipt from the USAID Mission in Mali, West Africa, of a $5 million grant to expand the activities of the current project “Transfer of Sorghum, Millet Production, Processing and Marketing Technologies in Mali.” Subsistence and resource poor farmers in West Africa are dependent on sorghum and millet as they serve as basic human foods and livestock feed. There continues to be a shortage of sorghum and millet grain in Mali, especially in the north. Mali’s ‘Hungry Season’ has been described as “that …

Putative Gene Promoter Sequences In The Chlorella Viruses, Lisa A. Fitzgerald, Philip Boucher, Giane Yanai-Balser, Karsten Suhre, Michael Graves, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Three short (7 to 9 nucleotides) highly conserved nucleotide sequences were identified in the putative promoter regions (150 bp upstream and 50 bp downstream of the ATG translation start site) of three members of the genus Chlorovirus, family Phycodnaviridae. Most of these sequences occurred in similar locations within the defined promoter regions. The sequence and location of the motifs were often conserved among homologous ORFs within the Chlorovirus family. One of these conserved sequences (AATGACA) is predominately associated with genes expressed early in virus replication.

University Of Nebraska Selected To Manage The New Sorghum/Millet And Other Grains Crsp, John Yohe, Kimberly Christiansen

INTSORMIL Impacts and Bulletins

Dr. John Yohe, INTSORMIL CRSP Program Director, has announced that the University of Nebraska (UNL) has been selected as the Management Entity for the new Sorghum/Millet and Other Grains CRSP. The award was based on national competition among U. S. universities. This CRSP is funded through a Leader with Associates Cooperative Agreement instrument as opposed to a grant under which INTSORMIL was funded since 1979. The INTSORMIL CRSP, Managed by UNL in partnership with Kansas State University, Purdue University, Texas A&M University, UNL and West Texas A&M University, ends June 30, 2007. The Leader with Associates Cooperative Agreement is effective …

Convergence Of Agriculture And Energy: Iii. Considerations In Biodiesel Production, Jon Van Gerpen, Allan Gray, Brent H. Shanks, Beth Calabotta, Drew Kershen, Alan Weber, Richard Joost, Todd A. Peterson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Concern about rising prices and unstable sources of petroleum fuels is driving the search for U.S. domestically produced, renewable transportation fuels, such as biodiesel. Federal incentives of up to $1.10 per gallon have been supplemented by additional incentives and mandated biodiesel use in many states. The Renewable Fuel Standard in the Energy Independence and Security Act of 2007 requires the domestic use of 1 billion gallons of biomass-based diesel fuel by 2012, most of which likely will be biodiesel.
U.S. biodiesel production is primarily from soybean oil, but recent high prices have forced many producers to use lower-cost feedstocks such …

Center For Grassland Studies Newsletter, Fall 2008, Volume 14, No. 4

Center for Grassland Studies: Newsletters

Contents:
Managing Grasslands for Structural Heterogeneity by Alexander Smart, Department of Animal and Range Sciences, South Dakota State University
Yellow Nutsedge: The Scourge of Lawns and Landscapes in Nebraska by Roch Gaussoin, Department of Agronomy and Horticulture, UNL
Targeting Habitat Dollars with Computers: Maximizing the Value of Data for Conservation by Drew Tyre, School of Natural Resources, UNL, with Naikoa Aguilar-Amustechegui, University of North Carolina-Wilmington and Max Post van der Burg, SNR, UNL
2008 Nebraska Grazing Conference Best Yet!

Enhanced Plant Nutrient Use Efficiency With Pgpr And Amf In An Integrated Nutrient Management System, A. O. Adesemoye, H. A. Torbert, J. W. Kloepper

Department of Plant Pathology: Faculty Publications

A 3-year field study was conducted with field corn from 2005 to 2007 to test the hypothesis that microbial inoculants that increase plant growth and yield can enhance nutrient uptake, and thereby remove more nutrients—especially N, P, and K—from the field as part of an integrated nutrient management system. The field trial evaluated microbial inoculants, which include a commercially available plant growth-promoting rhizobacteria (PGPR), arbuscular mycorrhiza fungi (AMF), and their combination across 2 tillage systems (no-till and conventional till) and 2 fertilization regimes (poultry litter and ammonium nitrate). Data were collected on plant height, yield (dry mass of ears and …

Early Attachment Sites For Shiga-Toxigenic Escherichia Coli O157:H7 In Experimentally Inoculated Weaned Calves, Evelyn A. Dean-Nystrom, William C. Stoffregen, Brad T. Bosworth, Harley W. Moon, Joachim F. Pohlenz

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Weaned 3- to 4-month-old calves were fasted for 48 h, inoculated with 10¹⁰ CFU of Shiga toxin-positive Escherichia coli (STEC) 0157:H7 strain 86-24 (STEC 0157) or STEC 091:H21 strain B2F1 (STEC 091), Shiga toxin-negative E. coli 0157:H7 strain 87-23 (Stx^- 0157), or a nonpathogenic control E. coli strain, necropsied 4 days postinoculation, and examined bacteriologically and histologically. Some calves were treated with dexamethasone (DEX) for 5 days (3 days before, on the day of, and 1 day after inoculation). STEC 0157 bacteria were recovered from feces, intestines, or gall bladders of 74% (40/55) of calves 4 days after …

Variations In Stomatal Traits Of 14 Bornean Tree Species Growing On Soils With Different Moisture Contents In Lambir Hills National Park, Whitney Logan Cannon

Department of Environmental Studies: Undergraduate Student Theses

The goal of this study was to look at variations in stomatal traits of tree species on soils with different moisture contents and fertility at Lambir Hills National Park. Stomates are important structures on the surface of leaves that mediate conduction of moisture and gassesin and out of the leaf. If stomatalt raits are important for regulation, then there should be variation in stomatal traits in regards to their soil specialization. The 14 Borneant ree speciess ampledi ncluded6 sandyl oam specialists6, clay specialistsa nd 2 generalistsfo und growing with equald istributionso n both sandyl oam and clay. Confocal microscopy was …

Sorghum & Pearl Millet In Zambia: Production Guide, [2006], Kimberley Christiansen

INTSORMIL Scientific Publications

U.S. government publication about sorghum and pearl millet production and sales in Zambia.

The Very-Long-Chain Hydroxy Fatty Acyl-Coa Dehydratase Pasticcino2 Is Essential And Limiting For Plant Development, Liên Bach, Louise V. Michaelson, Richard Haslam, Yannick Bellec, Lionel Gissot, Jessica Marion, Marco Da Costa, Jean-Pierre Boutin, Martine Miquel, Frédérique Tellier, Frederic Domergue, Jennifer E. Markham, Frederic Beaudoin, Johnathan A. Napier, Jean-Denis Faure

Department of Biochemistry: Faculty Publications

Very-long-chain fatty acids (VLCFAs) are synthesized as acyl-CoAs by the endoplasmic reticulum-localized elongase multiprotein complex. Two Arabidopsis genes are putative homologues of the recently identified yeast 3-hydroxy-acyl-CoA dehydratase (PHS1), the third enzyme of the elongase complex. We showed that Arabidopsis PASTICCINO2 (PAS2) was able to restore phs1 cytokinesis defects and sphingolipid long chain base overaccumulation. Conversely, the expression of PHS1 was able to complement the developmental defects and the accumulation of long chain bases of the pas2–1 mutant. The pas2–1 mutant was characterized by a general reduction of VLCFA pools in seed storage triacylglycerols, cuticular waxes, …

Protecting Sorghum Grain From Pests In Africa, Intsormil

INTSORMIL Impacts and Bulletins

Insect pests destroy more than 35% of stored grain worldwide and probably even more in Africa. Considering the current global food shortage this is a tremendous loss. To mitigate losses caused by insects in stored sorghum grain, INTSORMIL scientist Bonnie Pendleton of West Texas A&M University is collaborating with African entomologists to develop management strategies. Storage conditions are critical in preventing losses due to pests. A survey in Mali by IER scientist Niamoye Yaro Diarisso found that farmers in the Koulikoro and Ségou regions thresh sorghum and millet and store it in the form of grain. However, in the Sikasso …

Intsormil And Iiam Collaborate To Promote Sorghum Production In Mozambique, Intsormil

INTSORMIL Impacts and Bulletins

Sorghum is a major cereal grain in Mozambique with a cultivated area of 2.7 million hectares. It is considered as a food security crop in most of the provinces in the country, especially in regions where rainfall is a limiting factor for maize and rice production. However, due to a lack of seed and poor distribution of improved sorghums farmers continue to use their local varieties which have low productivity potential (0.2 - 0.6 ton/ha). To increase productivity and provide food security there is an urgent need for (1) the development of varieties with higher yield potential, (2) improved crop …

Epigenetic Transcriptional Repression Of Cellular Genes By A Viral Set Protein, Shiraz Mujtaba, Karishma L. Manzur, James Gurnon, Ming Kang, James L. Van Etten, Ming-Ming Zhou

Department of Plant Pathology: Faculty Publications

Viruses recruit host proteins to secure viral genome maintenance and replication. However, whether they modify host histones directly to interfere with chromatin-based transcription is unknown. Here we report that Paramecium bursaria chlorella virus 1 (PBCV-1) encodes a functional SET domain histone Lys methyltransferase (HKMTase) termed vSET, which is linked to rapid inhibition of host transcription after viral infection. We show that vSET is packaged in the PBCV-1 virion, and that it contains a nuclear localization signal and probably represses host transcription by methylating histone H3 at Lys 27 (H3K27), a modification known to trigger gene silencing in eukaryotes. We also …

Semi-Melanistic White-Tailed Deer In Northern Wisconsin, Christopher N. Jacques, Keith R. Mccaffery, Jonathan Jenks, John T. Baccus

The Prairie Naturalist

Melanistic color morphs of white-tailed deer (Odocoileus virginianus) are differentiated from other recognized color morphs by having uniform black hairs on the dorsal surface with subdued black hairs on the ventral surface, dark face and ears, a distinctive mid-dorsal stripe extending from the head to the apex of the tail, and a tail with black dorsally and white ventrally (Baccus and Posey 1999). Melanism results from the overproduction of the skin pigment melanin and is considered rare in white-tailed deer populations (Severinghaus and Cheatum 1956, Sauer 1984, Smith et al. 1984). Semi-melanistic deer have the same dark pelage …

A Case Study Of A Successful Lake Rehabilitation Project In South-Central Nebraska, Peter J. Spirk, Brad Newcomb, Keith D. Koupal

The Prairie Naturalist

Cottonmill Lake, a 17.4 ha impoundment located in Buffalo County, Nebraska, was a fishery dominated by common carp (Cyprinus carpio). As a result of the poor sportfish populations, angler participation in May and June of 1993 was low (503 ± 210 angler hours) and angler catch rates for all fish species (0.5 ± 0.4 fish/angler hour) was less than desired. In 1995, before rehabilitation, bluegill (Lepomis macrochirus) trap net catch per unit effort (CPUE) was 1.5 ± 0.9, largemouth bass (Micropterus salmoides) catch per hour of electrofishing was 8.0 ± 0.5, and channel catfish (Ictalurus punctatus) …

Non-Blackbird Avian Occurrence And Abundance In North Dakota Sunflower Fields, Dionn A. Schaaf, George M. Linz, Curt Doetkott, Mark W. Lutman, William J. Bleier

The Prairie Naturalist

Sunflower fields are well-documented as foraging habitat for fallmigrating blackbirds (Family Icteridae). There is, however, a paucity of information on the use of sunflower fields by non-blackbirds. We assessed non-blackbird use of 12 ripening sunflower fields in the Prairie Pothole Region of central North Dakota. From mid-August to mid-October 2000, we counted 4,129 individual birds, consisting of 22 families and 61 species, in the sample fields and within 5 m of the field edges. We saw the largest number of birds from 18 September to 27 September. The Family Emberizidae (sparrows) accounted for 26% of the species and 20% of …

The Prairie Naturalist. Volume 40, No. 3/4 September/December 2008, The Great Plains Natural Science Society

The Prairie Naturalist

WINTERKILL AND BIOMASS OF THE PAINTED TURTLE IN A SOUTH DAKOTA WETLAND. S. G. Platt, Z. Fast Horse, W. Cross, S. Mannel, and T. R. Rainwater

NON-BLACKBIRD AVIAN OCCURRENCE AND ABUNDANCE IN NORTH DAKOTA SUNFLOWER FIELDS. O. A. Schaaf, G. M. Linz, C. Ooetkott, M. W. Lutman, and W. J. Bleier

USING LOCAL KNOWLEDGE AND REMOTE SENSING TO MAP KNOWN AND POTENTIAL PRAIRIE-CHICKEN DISTRIBUTION IN KANSAS. M. E. Houts, R. O. Rodgers, R. D. Applegate, and W. H. Busby

A CASE STUDY OF A SUCCESSFUL LAKE REHABILITATION PROJECT IN SOUTH-CENTRAL NEBRASKA. P. J. Spirk, B. A. Newcomb, and K. O. …

Winterkill And Biomass Of The Painted Turtle In A South Dakota Wetland, Steven G. Platt, Zannita Fast Horse, Warren Cross, Sylvio Mannel, Thomas R. Rainwater

The Prairie Naturalist

Winterkill occurs when drought conditions expose hibernating turtles to desiccation and lethaly cold temperatures. Winterkill is thought to represent a major source of mortality in northern populations of the painted turtle (Chrysemys picta), but few field observations are available. We herein reported on catastrophic winterkill among western painted turtle (C. pieta bellii) at Limestone Butte Lake (LBL) in western South Dakota during the winter of 2003-2004. Additionally, we used the carcasses of winterkilled turtles (n = 86) to estimate the standing crop biomass of the painted turtle at LBL (0.6 kg/ha). This was the only estimate …

Nest Site Preference And Nesting Success Of Upland Sandpiper On Grazing Systems In East Central North Dakota-, Blane A. Klemek

The Prairie Naturalist

require ranchers to move their cattle throughout the range from one paddock to another, thereby allowing formerly grazed paddocks to regenerate. This is beneficial in a number of ways: overgrazing is reduced, cattle weight-gains are improved, suitable nesting cover for ground nesting birds is produced, and the overall health of the grassland is enhanced (Sedivec and Barker 1991). The objectives of my study were to investigate nesting success of the upland sandpiper as a function of grazing regime. Specifically, I was interested in comparing nesting success between RGS and non-rotational grazing systems (NRGS) and to examine relationships between nest site …