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Full-Text Articles in Plant Sciences
Analysis Of The Genome Of The Sexually Transmitted Insect Virus Helicoverpa Zea Nudivirus 2, John Burand, Woojin Kim, Claudio L. Afonso, Edan R. Tulman, Gerald F. Kutish, Zhiqiang Lu, Daniel L. Rock
Analysis Of The Genome Of The Sexually Transmitted Insect Virus Helicoverpa Zea Nudivirus 2, John Burand, Woojin Kim, Claudio L. Afonso, Edan R. Tulman, Gerald F. Kutish, Zhiqiang Lu, Daniel L. Rock
John Burand
The sexually transmitted insect virus Helicoverpa zea nudivirus 2 (HzNV-2) was determined to have a circular double-stranded DNA genome of 231,621 bp coding for an estimated 113 open reading frames (ORFs). HzNV-2 is most closely related to the nudiviruses, a sister group of the insect baculoviruses. Several putative ORFs that share homology with the baculovirus core genes were identified in the viral genome. However, HzNV-2 lacks several key genetic features of baculoviruses including the late transcriptional regulation factor, LEF-1 and the palindromic hrs, which serve as origins of replication. The HzNV-2 genome was found to code for three ORFs that …
Presence And Prevalence Of Viruses In Local And Migratory Honeybees (Apis Mellifera) In Massachusetts, John Burand, Anna Welch, Francis Drummond, Sunil Tewari, Anne Averill
Presence And Prevalence Of Viruses In Local And Migratory Honeybees (Apis Mellifera) In Massachusetts, John Burand, Anna Welch, Francis Drummond, Sunil Tewari, Anne Averill
John Burand
Migratory and local bees in Massachusetts were analyzed for seven viruses. Three were detected: black queen cell virus (BQCV), deformed wing virus (DWV), and sacbrood virus (SBV). DWV was most common, followed closely by BQCV and then by SBV. BQCV and SBV were present at significantly higher rates in the migratory bees assayed, bringing into question the impact that these bees have on the health of local bee populations.
Infection With The Insect Virus Hz-2v Alters Mating Behavior And Pheromone Production In Female Helicoverpa Zea Moths, John Burand, Weijia Tan, Woojin Kim, Satoshi Nojima, Wendell Roelofs
Infection With The Insect Virus Hz-2v Alters Mating Behavior And Pheromone Production In Female Helicoverpa Zea Moths, John Burand, Weijia Tan, Woojin Kim, Satoshi Nojima, Wendell Roelofs
John Burand
The effect of Hz-2V virus infection on the reproductive physiology and behavior of infected Helicoverpa zea female moths was examined. In the absence of males, infected females exhibited calling behavior and called as often but for shorter periods on average than control females. As expected, control females mated with males for extend periods when they were present and did not call after mating, while virus-infected females made many frequent contacts with males and continued to call even after these contacts. Virus-infected females were found to produce five to seven times more pheromone than control females and attracted twice as many …
In Vivo Dose-Response Of Insects To Hz-2v Infection, John P. Burand, Christopher P. Rallis
In Vivo Dose-Response Of Insects To Hz-2v Infection, John P. Burand, Christopher P. Rallis
John Burand
Background Hz-2V infection of female Helicoverpa zea moths is manifested as insects that are either sterile "agonadal" individuals with malformed reproductive tissues or fertile asymptomatic carriers which are capable of transmitting virus on to their progeny. Virus infected progeny arising from eggs laid by asymptomatic carrier females may themselves be either sterile agonadals or asymptomatic carriers. Results By injecting virus into female moths, a correlation was established between virus doses administered to the females and the levels of resulting asymptomatic and sterile progeny. Conclusions The results of these experiments indicate that high virus doses produced a higher level of agonadal …