Plant Sciences Commons^™

Proteomic Characterization And Cytotoxic Potential Of Proteins From Cuscuta (Cuscuta Epithymum (L.) Crude Herbal Product Against Mcf-7 Human Breast Cancer Cell Line, Umaima Akhtar, Yamna Khurshid, Bishoy El-Aarag, Basir Syed, Ishtiaq A. Khan, Keykavous Parang, Aftab Ahmed

Pharmacy Faculty Articles and Research

Background

The burden of breast cancer, the second leading cause of death worldwide, is increasing at an alarming rate. Cuscuta, used in traditional medicine for different ailments, including cancer, is known for containing phytochemicals that exhibit anticancer activity; however, the bioactivities of proteins from this plant remain unexplored. This study aimed to screen the cytotoxic potential of proteins from the crude herbal product of Cuscuta epithymum(L.) (CE) harvested from the host plants Alhagi maurorum and Medicago sativa.

Methods

The proteins from CE were extracted using a salting-out method, followed by fractionation with a gel filtration chromatography column. Gel-free shotgun …

Using Annual Forages To Replace Declining Cool-Season Grass Pasture Availability, Jonathon Jenkins

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

As land area in grain crop production increases in the US Midwest, perennial pasture availability decreases but the demand increases. Matching the seasonal growth patterns of C₃ and C₄ plant species to grow forages that are complementary allows producers to fill production gaps in otherwise monoculture systems and possibly increase overall productivity. One way to fill the forage production gap of C₃ grass dominated pastures is by producing a warm-season, annual forage in a separate field. However, with continual reductions in land availability, this may not be an option. Using two studies, our objectives were to improve …

Establishment Of Wildflower Islands To Enhance Roadside Health, Ecological Value, And Aesthetics, Jackson Ebbers

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Roadsides provide an abundant opportunity to increase connectivity of fragmented landscapes with diminishing floral resources for pollinating insects. The ecological value of these sites is often overlooked as quality habitat for pollinators, particularly monarch butterflies, which have been experiencing severe declines due to loss of habitat and loss of milkweeds that provide food for their larvae. Land managers across the nation are realizing the potential of roadsides to provide high quality floral resources for the benefit of insects and other wildlife. Current wildflower seed mixes used by state transportation departments are often low diversity and may only be implemented following …

Toward A Coordinated Understanding Of Hydro-Biogeochemical Root Functions In Tropical Forests For Application In Vegetation Models, Daniela F. Cusack, Bradley Christoffersen, Chris M. Smith-Martin, Kelly M. Andersen, Amanda L. Cordeiro, Katrin Fleischer, S. Joseph Wright, Nathaly R. Guerrero-Ramírez, Laynara F. Lugli, Lindsay A. Mcculloch, Mareli Sanchez-Julia, Sarah A. Batterman, Caroline Dallstream, Claire Fortunel, Laura Toro, Lucia Fuchslueger, Michelle Y. Wong, Daniela Yaffar, Joshua B. Fisher, Marie Arnaud, Lee H. Dietterich, Shalom D. Addo-Danso, Oscar J. Valverde-Barrantes, Monique Weemstra, Jing Cheng Ng, Richard J. Norby

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Tropical forest root characteristics and resource acquisition strategies are underrepresented in vegetation and global models, hampering the prediction of forest–climate feedbacks for these carbon-rich ecosystems. Lowland tropical forests often have globally unique combinations of high taxonomic and functional biodiversity, rainfall seasonality, and strongly weathered infertile soils, giving rise to distinct patterns in root traits and functions compared with higher latitude ecosystems. We provide a roadmap for integrating recent advances in our understanding of tropical forest belowground function into vegetation models, focusing on water and nutrient acquisition. We offer comparisons of recent advances in empirical and model understanding of root characteristics …

Hop Cone Drying For The Small Grower: Temperature And Airflow Considerations, Stacy A. Adams, David M. Mabie, Michael F. Kocher, David Jones

Department of Agronomy and Horticulture: Faculty Publications

Small hop growers without nearby processors for cone stripping and drying must attempt to do so on their own farm. Challenges exist for self-built drying systems, including drying capacity, processing speed, airflow direction, and maintaining quality during drying. Research-based recommendations are given for optimal temperature, sizing of drying vessel, maximum cone depth, and influences associated with airflow on processing uniformity and cone quality are presented.

Human Dimensions Of Woody Encroachment Management In Nebraska, Emily Rowen

School of Natural Resources: Dissertations, Theses, and Student Research

Woody plant encroachment (WPE) is a social-ecological problem that will challenge conservation professionals and agricultural producers to adapt their management strategies. This research first examined WPE from the perspective of individual conservation professionals through an online survey. Conservation professionals’ attitudes about adaptation to vegetation transitions, such as WPE, were of interest because these attitudes are one measure of how prepared this group is to respond to WPE. Hypothesized predictors of adaptation attitude were tested through linear regression modeling. These predictors included ecological change, observation of WPE, or risk perception. It was found that risk perception was the strongest predictor of …

A Global-Temporal Analysis On Phytophthora Sojae Resistance-Gene Efficacy, Austin G. Mccoy, Richard R. Belanger, Carl A. Bradley, Daniel G. Cerritos-Garcia, Vinicius C. Garnica, Loren Giesler, Pablo E. Grijalba, Eduardo Guillin, Maria A. Henriquez, Yong Min Kim, Dean K. Malvick, Rashelle L. Matthiesen, Santiago X. Mideros, Zachary A. Noel, Alison E. Robertson, Mitchell G. Roth, Clarice L. Schmidt, Damon L. Smith, Adam H. Sparks, Darcy E.P. Telenko, Vanessa Tremblay, Owen Wally, Martin I. Chilvers

Department of Plant Pathology: Faculty Publications

Plant disease resistance genes are widely used in agriculture to reduce disease outbreaks and epidemics and ensure global food security. In soybean, Rps (Resistance to Phytophthora sojae) genes are used to manage Phytophthora sojae, a major oomycete pathogen that causes Phytophthora stem and root rot (PRR) worldwide. This study aims to identify temporal changes in P. sojae pathotype complexity, diversity, and Rps gene efficacy. Pathotype data was collected from 5121 isolates of P. sojae, derived from 29 surveys conducted between 1990 and 2019 across the United States, Argentina, Canada, and China. This systematic review shows a loss of efficacy of …

2020-2021 Field Seasons Of Maize Gxe Project Within The Genomes To Fields Initiative, Dayane Cristina Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Alden Perkins, Dylan L. Schoemaker, Martin Costa, Kathryn J. Michel, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint-Garcia, Michael A. Gore, Candice N. Hirsch, Joseph E. Knoll, John Mckay, Richard Minyo, Seth C. Murray, James Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Erin E. Sparks, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Wenwei Xu, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Objectives: This release note describes the Maize GxE project datasets within the Genomes to Fields (G2F) Initiative. The Maize GxE project aims to understand genotype by environment (GxE) interactions and use the information collected to improve resource allocation efficiency and increase genotype predictability and stability, particularly in scenarios of variable environmental patterns. Hybrids and inbreds are evaluated across multiple environments and phenotypic, genotypic, environmental, and metadata information are made publicly available. Data description: The datasets include phenotypic data of the hybrids and inbreds evaluated in 30 locations across the US and one location in Germany in 2020 and 2021, soil …

Unraveling The Tapestry Of Indigenous Maize In North America: A Case Study Of Pawnee Ancestral Maize, Kahheetah Barnoskie

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Studies on Indigenous ancestral landrace maize in North America has significant historical and scientific importance. Indigenous peoples, such as the Pawnee people, have been cultivating maize for thousands of years, resulting in diverse varieties adapted to their local environments. This study aims to deepen the knowledge of Indigenous maize by examining specific varieties from the Pawnee, including a comparative analysis of the genetic makeup through DNA sequencing. This study used Genotyping by Target Sequencing (GBTS) method to examine the genetic variation and characteristics among the multiple varieties the Pawnee people once grew historically, providing valuable information about the evolutionary history …

Vulnerabilities Of Greater Prairie Chicken And Tier 1 At-Risk Species In Nebraska Caused By Grassland Transition To Woody Dominance, Robert Peterson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Woody plant encroachment is a global threat and has been transitioning grasslands to woody dominance at a biome scale. This threat is present in the Great Plains grassland biome which is currently experiencing grassland biome collapse as the alternative woody biome advances northwest. Nebraska, which contains the most intact temperate grassland in the world, is currently at the front lines of this large-scale transition making this state’s management decisions vital for the remaining grasslands and the species which rely on these ecosystems. In this study, we assess the vulnerability of Greater Prairie Chicken and Tier 1 at-risk species in Nebraska …

High Throughput Phenotyping: Field Based Triticale Breeding And Educational Resource Impact, Catherine Kay Mick

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Triticale (Triticosecale) is a multifunctional hybrid cereal crop that adopted the hardiness of rye and wheat's high-yielding and nutritional qualities. Plant breeding programs work to improve the quality and number of varieties available to producers through multiple rounds of evaluation and selection. However, traditional phenotyping methods are labor-intensive, time-consuming, and destructive, creating a phenotyping bottleneck. Remote sensing using unmanned aerial systems has the potential to alleviate this issue and change the evaluation of phenotypes in a breeding. Demand for educational resources to advance public awareness and prepare the workforce has increased with the utilization of more technology in …

Game-Theoretic Approaches To Optimal Resource Allocation And Defense Strategies In Herbaceous Plants, Molly R. Creagar

Department of Mathematics: Dissertations, Theses, and Student Research

Empirical evidence suggests that the attractiveness of a plant to herbivores can be affected by the investment in defense by neighboring plants, as well as investment in defense by the focal plant. Thus, allocation to defense may not only be influenced by the frequency and intensity of herbivory but also by defense strategies employed by other plants in the environment. We incorporate a neighborhood defense effect by applying spatial evolutionary game theory to optimal resource allocation in plants where cooperators are plants investing in defense and defectors are plants that do not. We use a stochastic dynamic programming model, along …

The Pseudomonas Syringae Pv. Tomato Dc3000 Effector Hopd1 Interferes With Cellular Dynamics Associated With The Function Of The Plant Immune Protein Atnhr2b, Luis Francisco Marín-Ponce, Catalina Rodríguez-Puerto, Perla Rocha-Loyola, Clemencia M. Rojas

Department of Plant Pathology: Faculty Publications

The plant pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) causes disease in tomato, in the model plant Arabidopsis thaliana, and conditionally in Nicotiana benthamiana. The pathogenicity of Pst DC3000 is mostly due to bacterial virulence proteins, known as effectors, that are translocated into the plant cytoplasm through the type III secretion system (T3SS). Bacterial type III secreted effectors (T3SEs) target plants physiological processes and suppress defense responses to enable and support bacterial proliferation. The Pst DC3000 T3SE HopD1 interferes with plant defense responses by targeting the transcription factor NTL9. This work shows that HopD1 also targets …

Does Selection For Seedling Tiller Number In Perennial Biomass Feedstocks Translate To Yield And Quality Improvements In Mature Swards?, Robert B. Mitchell, Kenneth P. Vogel, Susan Tunnell, James Stubbendieck

Department of Agronomy and Horticulture: Faculty Publications

Breeding for seedling traits in herbaceous perennial biomass feedstocks that translate into increased biomass yield or quality in established swards could accelerate the development of perennial grass cultivars for bioenergy or forage. In previously reported research, breeding for single large tillers (ST) or multiple tillers (MT, ≥3) six weeks after planting for two generations in big bluestem (Andropogon gerardii Vitman) and switchgrass (Panicum virgatum L.) base populations produced ST and MT populations that differed significantly for seedling and mature plant traits including biomass yield in spaced planted nurseries. Our objective was to evaluate these ST and MT populations in sward …

Volcanic Diffuse Volatile Emissions Tracked By Plant Responses Detectable From Space, Robert R. Bogue, Peter M. J. Douglas, Joshua B. Fisher, John Stix

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Volcanic volatile emissions provide information about volcanic unrest but are difficult to detect with satellites. Volcanic degassing affects plants by elevating local CO2 and H2O concentrations, which may increase photosynthesis. Satellites can detect plant health, or a reaction to photosynthesis, through a Normalized Difference Vegetation Index (NDVI). This can act as a potential proxy for detecting changes in volcanic volatile emissions from space. We tested this method by analyzing 185 Landsat 5 and 8 images of the Tern Lake thermal area (TLTA) in northeast Yellowstone caldera from 1984 to 2022. We compared the NDVI values of the thermal area with …

Center For Grassland Studies, November 2023

Center for Grassland Studies: Newsletters

Contents

Experiential Learning for Grassland Conservation by David Wedin

Center for Grassland Studies Policy Advisory Committee

Director’s Column by David Wedin

New Woody Invasions Ecologist Brings Additional Capacity for Grassland Conservation by Dillon Fogarty

Twidwell Racks-up awards for his work in Grassland Conservation by Geitner Simmons

Platte Basin Timelapse premieres New Film on Nine-Mile Prairie by David Wedin

CRAWL: Update on the Network for Integrated Agricultural Resilience Research by Craig Allen

CGS Outreach by Jerry Volesky

Center for Grassland Studies Awards 19 Scholarships Totaling $17,500 for 2023-2024 by David Wedin

Award winners:

Joseph O. Young Fund = Makenna Anderson, Makennen …

Multiple Light Signaling Pathways Control Solar Tracking In Sunflowers, Christopher J. Brooks, Hagop S. Atamian, Stacey L. Harmer

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Sunflowers are famous for their ability to track the sun throughout the day and then reorient at night to face east the following morning. This occurs by differential growth patterns, with the east sides of stems growing more during the day and the west sides of stems growing more at night. This process, termed heliotropism, is generally believed to be a specialized form of phototropism; however, the underlying mechanism is unknown. To better understand heliotropism, we compared gene expression patterns in plants undergoing phototropism in a controlled environment and in plants initiating and maintaining heliotropic growth in the field. We …

Genetic Analysis Of Basal Stalk Rot Resistance Introgressed From Wild Helianthus Petiolaris Into Cultivated Sunflower (Helianthus Annuus L.) Using An Advanced Backcross Population, Zahirul I. Talukder, William Underwood, Christopher G. Misar, Xuehui Li, Gerald J. Seiler, Xiwen Cai, Lili Qi

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sclerotinia sclerotiorum is a serious pathogen causing severe basal stalk rot (BSR) disease on cultivated sunflower (Helianthus annuus L.) that leads to significant yield losses due to insufficient resistance. The wild annual sunflower species H. petiolaris, commonly known as prairie sunflower is known for its resistance against this pathogen. Sunflower resistance to BSR is quantitative and determined by many genes with small effects on the resistance phenotype. The objective of this study was to identify loci governing BSR resistance derived from H. petiolaris using a quantitative trait loci (QTL) mapping approach.

Methods: BSR …

Effects Of Rootstock And Location On Open Field ‘Bhn 589’ And ‘Nebraska Wedding’ Grafted Tomato Yield, Raihanah H. Shonerd, Ashley A. Thompson, Samuel E. Wortman

Department of Agronomy and Horticulture: Faculty Publications

Grafted tomato (Solanum lycopersicum) production is popular, particularly in high tunnels, because grafted plants can mitigate soilborne disease incidence in highly infested soils and increase water and nutrient use efficiency and crop yield and quality. However, these potential benefits are not as well documented in open field production systems with less disease pressure. The objective of this study was to quantify the effect of tomato grafting on fruit yield, number, and size across 2 years (2018 and 2019) and three diverse open-field production environments in Nebraska (Lincoln, North Platte, and Dwight). At each location, a scion from one …

Evaluation Of Land Preparation Methods For Soil Stabilization, Revegetation, And Renewed Productivity In Semi-Arid Climates, Jarrett Lardy, North Dakota State University, Miranda Meehan, Aaron L. M. Daigh, James Staricka, Nathan Derby

Department of Agronomy and Horticulture: Faculty Publications

In the Williston Basin, land-preparation treatments have not been compared in side-by-side trials to evaluate performance for parameters such as runoff, erosion, and vegetation establishment. Thus, four treatments of wheat-straw crimping, land imprinting, wood-fiber hydromulch, and the combination of land imprinting and hydromulch were evaluated against a bare soil control in a replicated and randomized completed block field experiment near Williston, ND. Rainfall simulations were performed in September 2020 and June 2021 to examine the effectiveness of the treatments to reduce runoff and sediment losses. Vegetation establishment was also evaluated in August 2021. The wheat straw treatment reduced the equivalent …

Deleterious Mutations Predicted In The Sorghum (Sorghum Bicolor) Maturity (Ma) And Dwarf (Dw) Genes From Whole‑Genome Resequencing, Nathan P. Grant, John J. Toy, Deanna L. Funnell, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

In sorghum [Sorghum bicolor (L.) Moench] the Maturity (Ma1, Ma2, Ma3, Ma4, Ma5, Ma6) and Dwarf (Dw1, Dw2, Dw3, Dw4) loci, encode genes controlling flowering time and plant height, respectively, which are critical for designing sorghum ideotypes for a maturity timeframe and a harvest method. Publicly available whole-genome resequencing data from 860 sorghum accessions was analyzed in silico to identify genomic variants at 8 of these loci (Ma1, Ma2, Ma3, Ma5, Ma6, Dw1, Dw2, Dw3) to identify novel loss …

Did Cover Crop Or Animal Manure Ameliorate Corn Residue Removal Effects On Soil Mechanical Properties After 10 Years?, Hans W. Klopp, Humberto Blanco-Canqui, Michael Sindelar, Virginia L. Jin, Marty R. Schmer, Richard B. Ferguson

Department of Agronomy and Horticulture: Faculty Publications

Crop residue removal may negatively affect soil mechanical properties, which are key components of soil quality. To evaluate potential long-term effects, we assessed the 10-yr impact of corn (Zea mays L.) residue removal (59 % of non-grain biomass annually) on surface soil mechanical properties (0–20 cm). We also evaluated whether adding carbon (C) amendments, such as using a winter rye (Secale cereale L.) cover crop or surface-applying cattle manure (24 Mg ha− 1 biannually) can ameliorate the effects of crop residue removal. This long-term study was under irrigated no-till continuous corn on a silt loam soil in south-central …

Dynamic Reconfiguration Of Switchgrass Proteomes In Response To Rust (Puccinia Novopanici) Infection, Nathan A. Palmer, Sophie Alvarez, Michael J. Naldrett, Anthony Muhle, Gautam Sarath, Serge J. Edmé, Satyanarayana Tatineni, Robert B. Mitchell, Gary Yuen

Department of Plant Pathology: Faculty Publications

Switchgrass (Panicum virgatum L.) can be infected by the rust pathogen (Puccinia novopanici) and results in lowering biomass yields and quality. Label-free quantitative proteomics was conducted on leaf extracts harvested from non-infected and infected plants from a susceptible cultivar (Summer) at 7, 11, and 18 days after inoculation (DAI) to follow the progression of disease and evaluate any plant compensatory mechanisms to infection. Some pustules were evident at 7 DAI, and their numbers increased with time. However, fungal DNA loads did not appreciably change over the course of this experiment in the infected plants. In total, 3830 …

Is Climate Change Causing The Range Contraction Of Cape Rock-Jumpers (Chaetops Frenatus)?, Gregory D. Duckworth, Raquel A. Garcia, Rheinhardt Scholtz, Res Altwegg

Department of Agronomy and Horticulture: Faculty Publications

Species distribution models often suggest strong links between climate and species' distribution boundaries and project large distribution shifts in response to climate change. However, attributing distribution shifts to climate change requires more than correlative models. One idea is to examine correlates of the processes that cause distribution shifts, namely colonization and local extinction, by using dynamic occupancy models. The Cape Rock-jumper (Chaetops frenatus) has disappeared over most of its distribution where temperatures are the highest. We used dynamic occupancy models to analyse Cape Rock-jumper distribution with respect to climate (mean temperature and precipitation over the warmest annual quarter), …

Dicamba Off-Target Movement From Applications On Soybeans At Two Growth Stages, Greg R. Kruger, Guilherme S. Alves, Kasey Schroeder, Jeffrey A. Golus, Daniel B. Reynolds, Darrin M. Dodds, Ashli E. Brown, Bradley K. Fritz, Wesley C. Hoffmann

Department of Agronomy and Horticulture: Faculty Publications

The objective of this study was to evaluate dicamba off-target movement during and after applications over soybean at two growth stages. Dicamba-tolerant soybean [Glycine max (L.) Merr.] at V3 and R1 growth stages in Nebraska and Mississippi fields were treated with diglycolamine salt of dicamba (560 g ae ha⁻¹), potassium salt of glyphosate (1260 g ae ha⁻¹), and a drift-reducing adjuvant (0.5% v v⁻¹). Filter papers positioned outside the sprayed area were used to determine primary movement and air samplers positioned at the center of sprayed area were used to calculate dicamba flux …

A Follow-Up Survey To Assess Stakeholders’ Perspectives On Weed Management Challenges And Current Practices In Nebraska, Usa, Shawn T. Mcdonald, Debalin Sarangi, Jennifer M. Rees, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Stakeholders across the state of Nebraska, USA, were surveyed in 2019–2020 to assess problem weeds and weed management practices in agronomic crops. A total of 420 complete responses were obtained across four Nebraska districts (Northeast, Panhandle, Southeast, and West Central). Accumulated across the state, 65.5% of farmed or scouted crop ground in Nebraska was under no-till production, with the major crops being corn and soybean representing 39.3% and 30.7% of agronomic crop production area, respectively. Palmer amaranth, horseweed, waterhemp, kochia, and giant ragweed were ranked the most problematic weeds. In a 2014–2015 survey, Palmer amaranth was the sixth most problematic …

The Evolving Definition Of Plant Cell Type, Sahand Amini, Jeffrey J. Doyle, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

In 1665 Robert Hooke, looking at cork through his microscope, discovered that plants are composed of elementary structures he named “cells”. Variation in the expression of a single genome in a complex eukaryotic organism guides the initiation, maturation, physiology, and biochemistry of cells with different shapes and sizes, playing different structural and functional roles in space and time. How many kinds of cells—”cell types”— an organism possesses of course depends on the organism’s cellular complexity, but the plasticity within a cell type fuels the emergence of the concept of cell state (Figure 1). The transition between cell states is driven …

Shortening Harvest Interval, Reaping Benefits? A Study On Harvest Practices In Oil Palm Smallholder Farming Systems In Indonesia, Rosanne E. De Vos, Lisa Nurfalah, Fatima A. Tenorio, Ya Li Lim, Juan Pablo Monzon, Christopher R. Donough, Hendra Sugianto, Asri A. Dwiyahreni, Nurul L. Winarni, Nadia Mulani, Gilang Ramadhan, Muhammad Ali Imran, Antonius P. Tito, Pandu Sulistiawan, Muhammad Khoirul, Rana Farrasati, Iput Pradiko, Patricio Grassini, Maja Slingerland

Department of Agronomy and Horticulture: Faculty Publications

CONTEXT: Smallholders are responsible for a large share of global palm oil production. Yet, in Indonesia, the main palm oil producing country, smallholders’ yields remain low. Better management practices, including short harvest interval (HI, the number of days between two harvest rounds), could help to raise smallholder yields. However, at present, HI is long in smallholder fields and the drivers underlying this phenomenon are poorly understood.

OBJECTIVE: We explored agronomic, socio-economic, and institutional factors that underlie harvesting practices in independent oil palm smallholder farming systems in Indonesia to assess scope for sustainable intensification through shorter HI and reduced …

Exotic Herbivores And Fire Energy Drive Standing Herbaceous Biomass But Do Not Alter Compositional Patterns In A Semiarid Savanna Ecosystem, Virginia D. Preiss, Carissa L. Wonkka, Devan A. Mcgranahan, Alexandra G. Lodge, Matthew B. Dickinson, Kathleen L. Kavanagh, Heath D. Starns, Douglas R. Tolleson, Morgan L. Treadwell, Dirac L. Twidwell Jr, William E. Rogers

Department of Agronomy and Horticulture: Faculty Publications

Questions: Fire regime alterations are pushing open ecosystems worldwide past tipping points where alternative steady states characterized by woody dominance prevail. This reduces the frequency and intensity of surface fires, further limiting their effectiveness for controlling cover of woody plants. In addition, grazing pressure (exotic or native grazers) can reinforce woody encroachment by potentially reducing fine-fuel loads. We investigated the effects of different fire energies on the herbaceous plant community, together with mammalian wildlife herbivory (exotic and native combined) exclusion, to inform best management practices.

Location: Texas semi-arid savanna, southern Great Plains, USA.

Methods: We conducted an …

Targeted Sequencing Of The Panicum Miliaceum Gene Space And Genotyping Of Variant Sites From Population Genetics Studies, Combined In A Single Assay, As A Tool For Broomcorn Millet Assisted Breeding, Gabriele Magris, Serena Foria, Silvano Ciani, Dipak K. Santra, Ombretta Polenghi, Virna Cerne, Michele Morgante, Gabriele Di Gaspero

Department of Agronomy and Horticulture: Faculty Publications

Broomcorn millet (Panicum miliaceum L.) is a prehistorical cereal, today cultivated as a minor crop with low yields but with a renewed interest for its high water use effciency and gluten-free grains. To reverse the downward trend in broomcorn millet cultivation, the crop needs genetic improvement and creation of novel genetic variation to increase productivity. In order to facilitate genomics-assisted breeding, we designed a reduced representation genome-sequencing assay that investigates 1.8% of the nuclear DNA in a targeted and reproducible way, with an intensity of genomic sampling that is a direct function of local recombination rate. We used this …