Plant Sciences Commons^™

Crop Dna Extraction With Lab-Made Magnetic Nanoparticles, Haichuan Wang, Xueqi Zhao, Li Tan, Junwei Zhu, David Hyten

Department of Agronomy and Horticulture: Faculty Publications

Molecular breeding methods, such as marker-assisted selection and genomic selection, require high-throughput and cost-effective methods for isolating genomic DNA from plants, specifically from crop tissue or seed with high polysaccharides, lipids, and proteins. A quick and inexpensive high-throughput method for isolating genomic DNA from seed and leaf tissue from multiple crops was tested with a DNA isolation method that combines CTAB extraction buffer and lab-made SA-coated magnetic nanoparticles. This method is capable of isolating quality genomic DNA from leaf tissue and seeds in less than 2 hours with fewer steps than a standard CTAB extraction method. The yield of the …

Soybean Genetics, Genomics, And Breeding For Improving Nutritional Value And Reducing Antinutritional Traits In Food And Feed, William M. Singer, Yi-Chen Lee, Zachary Shea, Caio Canella Vieira, Dongho Lee, Xiaoying Li, Mia Cunicelli, Shaila S. Kadam, Mohammad Amir Waseem Khan, Grover Shannon, M. A. Rouf Mian, Henry T. Nguyen, Bo Zhang

Agriculture Faculty Publications

Soybean [Glycine max(L.) Merr.] is a globally important crop due to its valuable seed composition, versatile feed, food, and industrial end-uses, and consistent genetic gain. Successful genetic gain in soybean has led to widespread adaptation and increased value for producers, processors, and consumers. Specific focus on the nutritional quality of soybean seed composition for food and feed has further elucidated genetic knowledge and bolstered breeding progress. Seed components are historical and current targets for soybean breeders seeking to improve nutritional quality of soybean. This article reviews genetic and genomic foundations for improvement of nutritionally important traits, such as protein and …

Microbiome-Enabled Genomic Selection Improves Prediction Accuracy For Nitrogen-Related Traits In Maize, Zhikai Yang, Tianjing Zhao, Hao Cheng, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

Root-associated microbiomes in the rhizosphere (rhizobiomes) are increasingly known to play an important role in nutrient acquisition, stress tolerance, and disease resistance of plants. However, it remains largely unclear to what extent these rhizobiomes contribute to trait variation for different genotypes and if their inclusion in the genomic selection protocol can enhance prediction accuracy. To address these questions, we developed a microbiome-enabled genomic selection method that incorporated host SNPs and amplicon sequence variants from plant rhizobiomes in a maize diversity panel under high and low nitrogen (N) field conditions. Our cross-validation results showed that the microbiome-enabled genomic selection model significantly …

Origin, Rise And Development Of American Upland Cotton And Their Status At Present. Second Edition, Ed. By F.M. Bourland, J. O. Ware

Research Reports and Research Bulletins

American Upland cotton (Gossypium hirsutum) is a Dixie product. Although the stocks of the species were brought from elsewhere, new types (through series of adaptational changes) formed this distinctive group—the final characteristics of which are a product of the Cotton Belt of the United States. These biological processes were considerably aided by man and the steps of development possibly were about as follows: (a) natural selection took place in the earlier introductions, (b) seed were saved from the more choice stocks, (c) series of subsequent introductions were obtained from the better sources, (d) these were acclimatized and the superior ones …

Assessing The Morphological And Physiological Traits Of Smooth Brome Pastures Under Long Term Grazing And Nutrient Enrichment In Eastern Nebraska, Hassan Shehab

School of Natural Resources: Dissertations, Theses, and Student Research

Climate change together with the increase in demands for food, feed, fuel, and fiber are becoming a threat to sustainability and resilience of agriculture and pasture lands. Bromus inermis (smooth bromegrass) dominates pastures for cattle grazing in Eastern Nebraska, US, where it is planted in monocultures, and is considered high quality forage because of its palatability and high nutritional value for cattle, especially under intensive management practices. Sustainable management of these pastures is key to long-term resilience. This study aims to assess the performance of smooth bromegrass pastures to the combined effects of long-term management practices (since 2005) of fertilization …

Corn And Grain Sorghum Performance Tests 2023, J. F. Carlin, R. B. Mulloy, R. D. Bond, J. C. Mccoy

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

The 2023 corn performance tests contained 46 hybrids and were conducted at the Northeast Rice Research and Extension Center (NERREC) at Harrisburg, the Northeast Rice Research and Extension Center (NEREC) at Keiser, the Lon Mann Cotton Research Station (LMCRS) near Marianna, the Rohwer Research Station (RRS) near Rohwer, and the Rice Research and Extension Center (RREC) …

2020-2021 Field Seasons Of Maize Gxe Project Within The Genomes To Fields Initiative, Dayane Cristina Lima, Alejandro Castro Aviles, Ryan Timothy Alpers, Alden Perkins, Dylan L. Schoemaker, Martin Costa, Kathryn J. Michel, Shawn Kaeppler, David Ertl, Maria Cinta Romay, Joseph L. Gage, James Holland, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint-Garcia, Michael A. Gore, Candice N. Hirsch, Joseph E. Knoll, John Mckay, Richard Minyo, Seth C. Murray, James Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Erin E. Sparks, Peter Thomison, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Jacob D. Washburn, Teclemariam Weldekidan, Wenwei Xu, Natalia De Leon

Department of Agronomy and Horticulture: Faculty Publications

Objectives: This release note describes the Maize GxE project datasets within the Genomes to Fields (G2F) Initiative. The Maize GxE project aims to understand genotype by environment (GxE) interactions and use the information collected to improve resource allocation efficiency and increase genotype predictability and stability, particularly in scenarios of variable environmental patterns. Hybrids and inbreds are evaluated across multiple environments and phenotypic, genotypic, environmental, and metadata information are made publicly available. Data description: The datasets include phenotypic data of the hybrids and inbreds evaluated in 30 locations across the US and one location in Germany in 2020 and 2021, soil …

Evaluation Of Vegetative Indices To Determine Canopy Ground Cover For Winter Survival And Hybrid Necrosis In Winter Wheat, Micheal Young

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The benefit of unmanned aircraft systems and image processing methods in agronomic research across numerous crops has been well documented as has the importance of wheat, Triticum aestivum L., on the global food supply. Hence there is great interest in digital solutions applied to aspects of wheat breeding. A major trait of importance to winter wheat breeders in higher latitudes is winter survival, which can result in poor yield and performance if lines do not survive extreme cold. Scoring winter survival is most commonly based on visual score of 0% to 100% with the higher percentage conveying higher winter survival …

High Throughput Phenotyping: Field Based Triticale Breeding And Educational Resource Impact, Catherine Kay Mick

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Triticale (Triticosecale) is a multifunctional hybrid cereal crop that adopted the hardiness of rye and wheat's high-yielding and nutritional qualities. Plant breeding programs work to improve the quality and number of varieties available to producers through multiple rounds of evaluation and selection. However, traditional phenotyping methods are labor-intensive, time-consuming, and destructive, creating a phenotyping bottleneck. Remote sensing using unmanned aerial systems has the potential to alleviate this issue and change the evaluation of phenotypes in a breeding. Demand for educational resources to advance public awareness and prepare the workforce has increased with the utilization of more technology in …

Unraveling The Tapestry Of Indigenous Maize In North America: A Case Study Of Pawnee Ancestral Maize, Kahheetah Barnoskie

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Studies on Indigenous ancestral landrace maize in North America has significant historical and scientific importance. Indigenous peoples, such as the Pawnee people, have been cultivating maize for thousands of years, resulting in diverse varieties adapted to their local environments. This study aims to deepen the knowledge of Indigenous maize by examining specific varieties from the Pawnee, including a comparative analysis of the genetic makeup through DNA sequencing. This study used Genotyping by Target Sequencing (GBTS) method to examine the genetic variation and characteristics among the multiple varieties the Pawnee people once grew historically, providing valuable information about the evolutionary history …

Arkansas Soybean Research Studies 2022, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading soybean-producing state in the mid-southern United States. Arkansas ranked 11th in soybean production in 2022 when compared to the other soybean-producing states in the U.S. The state represented 3.04% of the total U.S. soybean production and 3.64% of the total acres planted in soybean in 2022. The 2022 state soybean average yield was 52.0 bushels per acre, tying the previous state yield record of 52 bushels per acre set in 2021. The top five soybean-producing counties in 2022 were Mississippi, Crittenden, Phillips, Poinsett, and Arkansas (Table 1). These five counties accounted for over 35.7% of the …

Responses Of Maize To Different Irrigation Regimes In Semi-Arid Western Nebraska, Swathi Palle

Department of Biological Systems Engineering: Dissertations and Theses

As the “Cornhusker State”, Maize (Zea mays) is an important crop in Nebraska. However, farmers in the state are challenged by unstable supply of surface water and limited groundwater resources. A better understanding of maize's response to water stress that occurs at different growth stages can help implement the best irrigation practices that conserve water while maintaining yields. In this study, we conducted field experiments at the Panhandle Research, Extension, and Education Center to compare the responses of maize to eight irrigation treatments which included both limited and deficit irrigation regimes during 2022 and 2023 growing seasons. Specifically, …

Do Organic Farming Practices Improve Soil Physical Properties?, Humberto Blanco-Canqui, Sabrina J. Ruis, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Organic farming (OF) is a reemerging system that could address food security and adverse environmental footprints of conventional farming (CF). However, how OF affects the soil physical environment, an essential pillar for soil ecosystem service delivery, is not well understood. This paper (1) reviews published global literature up to 13 July 2023 regarding the impacts of OF on soil physical properties compared with CF and (2) underlines research needs. Literature indicates OF improves some soil physical properties relative to CF although studies on some properties were few. Specifically, OF increased wet aggregate stability, saturated hydraulic conductivity, and plant available water …

Root-Associated Bacterial Communities And Root Metabolite Composition Are Linked To Nitrogen Use Efficiency In Sorghum, Yen Ning Chai, Yunhui Qi, Emily Goren, Dawn Chiniquy, Amy M. Sheflin,, Susannah G. Tringe, Jessica E. Prenni, Peng Liu, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

The development of cereal crops with high nitrogen use efficiency (NUE) is a priority for worldwide agriculture. In addition to conventional plant breeding and genetic engineering, the use of the plant microbiome offers another approach to improving crop NUE. To gain insight into the bacterial communities associated with sorghum lines that differ in NUE, a field experiment was designed comparing 24 diverse Sorghum bicolor lines under sufficient and deficient nitrogen (N). Amplicon sequencing and untargeted gas chromatography–mass spectrometry were used to characterize the bacterial communities and the root metabolome associated with sorghum genotypes varying in sensitivity to low N. We …

Does Selection For Seedling Tiller Number In Perennial Biomass Feedstocks Translate To Yield And Quality Improvements In Mature Swards?, Robert B. Mitchell, Kenneth P. Vogel, Susan Tunnell, James Stubbendieck

Department of Agronomy and Horticulture: Faculty Publications

Breeding for seedling traits in herbaceous perennial biomass feedstocks that translate into increased biomass yield or quality in established swards could accelerate the development of perennial grass cultivars for bioenergy or forage. In previously reported research, breeding for single large tillers (ST) or multiple tillers (MT, ≥3) six weeks after planting for two generations in big bluestem (Andropogon gerardii Vitman) and switchgrass (Panicum virgatum L.) base populations produced ST and MT populations that differed significantly for seedling and mature plant traits including biomass yield in spaced planted nurseries. Our objective was to evaluate these ST and MT populations in sward …

Genetic Analysis Of Basal Stalk Rot Resistance Introgressed From Wild Helianthus Petiolaris Into Cultivated Sunflower (Helianthus Annuus L.) Using An Advanced Backcross Population, Zahirul I. Talukder, William Underwood, Christopher G. Misar, Xuehui Li, Gerald J. Seiler, Xiwen Cai, Lili Qi

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sclerotinia sclerotiorum is a serious pathogen causing severe basal stalk rot (BSR) disease on cultivated sunflower (Helianthus annuus L.) that leads to significant yield losses due to insufficient resistance. The wild annual sunflower species H. petiolaris, commonly known as prairie sunflower is known for its resistance against this pathogen. Sunflower resistance to BSR is quantitative and determined by many genes with small effects on the resistance phenotype. The objective of this study was to identify loci governing BSR resistance derived from H. petiolaris using a quantitative trait loci (QTL) mapping approach.

Methods: BSR …

Effects Of Rootstock And Location On Open Field ‘Bhn 589’ And ‘Nebraska Wedding’ Grafted Tomato Yield, Raihanah H. Shonerd, Ashley A. Thompson, Samuel E. Wortman

Department of Agronomy and Horticulture: Faculty Publications

Grafted tomato (Solanum lycopersicum) production is popular, particularly in high tunnels, because grafted plants can mitigate soilborne disease incidence in highly infested soils and increase water and nutrient use efficiency and crop yield and quality. However, these potential benefits are not as well documented in open field production systems with less disease pressure. The objective of this study was to quantify the effect of tomato grafting on fruit yield, number, and size across 2 years (2018 and 2019) and three diverse open-field production environments in Nebraska (Lincoln, North Platte, and Dwight). At each location, a scion from one …

Evaluation Of Land Preparation Methods For Soil Stabilization, Revegetation, And Renewed Productivity In Semi-Arid Climates, Jarrett Lardy, North Dakota State University, Miranda Meehan, Aaron L. M. Daigh, James Staricka, Nathan Derby

Department of Agronomy and Horticulture: Faculty Publications

In the Williston Basin, land-preparation treatments have not been compared in side-by-side trials to evaluate performance for parameters such as runoff, erosion, and vegetation establishment. Thus, four treatments of wheat-straw crimping, land imprinting, wood-fiber hydromulch, and the combination of land imprinting and hydromulch were evaluated against a bare soil control in a replicated and randomized completed block field experiment near Williston, ND. Rainfall simulations were performed in September 2020 and June 2021 to examine the effectiveness of the treatments to reduce runoff and sediment losses. Vegetation establishment was also evaluated in August 2021. The wheat straw treatment reduced the equivalent …

Irrigation Increases On-Farm Soybean Yields In Water-Limited Environments Without A Trade-Off In Seed Protein Concentration, Walter D. Carciochi, Patricio Grassini, Seth Naeve, James E. Specht, Mitiku Mamo, Ron Seymour, Aaron Nygren, Nathan Mueller, Sarah Sivits, Christopher A. Proctor, Jenny Rees, Todd Whitney, N. Cafaro La Menza

Department of Agronomy and Horticulture: Faculty Publications

Context or problem: A trade-off between seed protein concentration (SPC) and yield has been reported for soybean. Therefore, assessing management practices that can nullify this trade-off is relevant to avoid further declines in SPC in the future as yield continues to increase. While the positive effect of irrigation on yield is well documented, only a few studies have assessed the impact of irrigation on SPC, showing conflicting results.

Objective or research question: The objective was to determine if the trade-off between seed yield and SPC persists when irrigation is applied and how management, soil, and weather factors influence the trade-off. …

Deleterious Mutations Predicted In The Sorghum (Sorghum Bicolor) Maturity (Ma) And Dwarf (Dw) Genes From Whole‑Genome Resequencing, Nathan P. Grant, John J. Toy, Deanna L. Funnell, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

In sorghum [Sorghum bicolor (L.) Moench] the Maturity (Ma1, Ma2, Ma3, Ma4, Ma5, Ma6) and Dwarf (Dw1, Dw2, Dw3, Dw4) loci, encode genes controlling flowering time and plant height, respectively, which are critical for designing sorghum ideotypes for a maturity timeframe and a harvest method. Publicly available whole-genome resequencing data from 860 sorghum accessions was analyzed in silico to identify genomic variants at 8 of these loci (Ma1, Ma2, Ma3, Ma5, Ma6, Dw1, Dw2, Dw3) to identify novel loss …

The Silver Bullet That Wasn’T: Rapid Agronomic Weed Adaptations To Glyphosate In North America, Christopher Landau, Kevin Bradley, Erin Burns, Michael Flessner, Karla Gage, Aaron Hager, Joseph Ikley, Prashant Jha, Amit Jhala, Paul O. Johnson, William Johnson, Sarah Lancaster, Travis Legleiter, Dwight Lingenfelter, Mark Loux, Eric Miller, Jason Norsworthy, Micheal Owen, Scott Nolte, Debalin Sarangi, Peter Sikkema, Christy Sprague, Mark Vangessel, Rodrigo Werle, Bryan Young, Martin M. Williamsii

Department of Agronomy and Horticulture: Faculty Publications

The rapid adoption of glyphosate-resistant crops at the end of the 20th century caused a simplification of weed management that relied heavily on glyphosate for weed control. However, the effectiveness of glyphosate has diminished. A greater understanding of trends related to glyphosate use will shed new light on weed adaptation to a product that transformed global agriculture. Objectives were to (1) quantify the change in weed control efficacy from postemergence (POST) glyphosate use on troublesome weeds in corn and soybean and (2) determine the extent to which glyphosate preceded by a preemergence (PRE) improved the efficacy and consistency of weed …

Arkansas Wheat Performance Tests 2022-2023, J. F. Carlin, R. B. Mulloy, R. D. Bond

Arkansas Agricultural Experiment Station Research Series

Wheat variety performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture’s Arkansas Crop Variety Improvement Program.

The tests provide information to companies developing cultivars and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating variety recommendations for small-grain producers. The tests were conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Pine Tree Research Station near Colt, and the Rohwer Research Station near Rohwer. Specific location and cultural practice information accompany each …

Portugal To New Mexico: Investigating The Applicability Of Syntropic Agriculture To A Semi-Arid Continental Climate, Arielle Nathan

Independent Study Project (ISP) Collection

Conventional industrial agriculture has numerous detrimental effects on the environment including high carbon emissions, pollution and overconsumption of water, soil degradation, and reduction of biodiversity (FAO, 2018). Large-scale alternatives are needed to combat these negative consequences and provide climate solutions. Syntropic Agriculture is a promising farming alternative especially in arid systems subject to degraded soils and desertification. New Mexico is a semi-arid continental climate in a prolonged period of drought and predicted to experience worsening agricultural conditions due to desertification, wildfire intensification, and exacerbated water scarcity. In an area with similar agricultural challenges, a project in Mértola, Portugal (Terra Sintrópica) …

The Truth Behind Avocado Expansion In The Algarve: A Synthesis Of Public, Environmental Associations, And Government Perspectives And Response, Mandy Ausman

Independent Study Project (ISP) Collection

Avocado expansion has exponentially increased in the Algarve region of southern Portugal in recent years. The surge in expansion has provoked contention and discrepancy in the media regarding how and if avocado production can persist in the water-scarce region due to worsening drought conditions related to climate change coupled with the fruit’s significant water footprint. This paper aims to synthesize the arguments and response of the public, environmental associations, and government agencies to the expansion of avocado production in the water- scarce region of the Algarve, highlight the region’s current challenges of avocado expansion and water management regulation, and analyze …

Assessing The Performance Of Agricultural Systems In The Inland And Coastal Regions Of Northern Portugal Using Indicators: Establishing The Foundation Towards Better Agricultural Practices, Genevieve Gehlken

Independent Study Project (ISP) Collection

This study conducts a comprehensive evaluation of agricultural systems in the distinct coastal and inland regions of Northern Portugal. Employing key indicators, including soil erosion, nitrogen surplus, irrigation practices, Agricultural Gross Domestic Product (GDP), and crop yield, the analysis reveals statistically significant disparities and unique challenges in each region. The coastal areas, marked by higher agricultural GDP, exhibit distinct economic dependencies on agriculture, while the inland regions face challenges related to water scarcity, soil erosion, and rural isolation. The looming threat of climate change adds complexity to these dynamics, further highlighting the need for region-specific, sustainable agricultural practices. In the …

Optimizing Crop Productivity And Fertility Practices In Intermediate Wheatgrass, Roberta Bianchin Rebesquini

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The intensive management associated with many annual crops often includes recurring tillage, fertilization, and pesticide applications, which contribute to environmental concerns such as water pollution and soil erosion. Intermediate wheatgrass (Thinopyrum intermedium), recognized under the trade name Kernza®, is a perennial grass that can be managed to produce grain and biomass while providing desired environmental benefits such as soil conservation and nutrient cycling. There has been limited research on best management practices and crop productivity for this alternative dual-use crop in Nebraska. A field experiment was conducted beginning in 2021 to assess nitrogen (N), phosphorus (P), and potassium (K) management …

Did Cover Crop Or Animal Manure Ameliorate Corn Residue Removal Effects On Soil Mechanical Properties After 10 Years?, Hans W. Klopp, Humberto Blanco-Canqui, Michael Sindelar, Virginia L. Jin, Marty R. Schmer, Richard B. Ferguson

Department of Agronomy and Horticulture: Faculty Publications

Crop residue removal may negatively affect soil mechanical properties, which are key components of soil quality. To evaluate potential long-term effects, we assessed the 10-yr impact of corn (Zea mays L.) residue removal (59 % of non-grain biomass annually) on surface soil mechanical properties (0–20 cm). We also evaluated whether adding carbon (C) amendments, such as using a winter rye (Secale cereale L.) cover crop or surface-applying cattle manure (24 Mg ha− 1 biannually) can ameliorate the effects of crop residue removal. This long-term study was under irrigated no-till continuous corn on a silt loam soil in south-central …

Is Climate Change Causing The Range Contraction Of Cape Rock-Jumpers (Chaetops Frenatus)?, Gregory D. Duckworth, Raquel A. Garcia, Rheinhardt Scholtz, Res Altwegg

Department of Agronomy and Horticulture: Faculty Publications

Species distribution models often suggest strong links between climate and species' distribution boundaries and project large distribution shifts in response to climate change. However, attributing distribution shifts to climate change requires more than correlative models. One idea is to examine correlates of the processes that cause distribution shifts, namely colonization and local extinction, by using dynamic occupancy models. The Cape Rock-jumper (Chaetops frenatus) has disappeared over most of its distribution where temperatures are the highest. We used dynamic occupancy models to analyse Cape Rock-jumper distribution with respect to climate (mean temperature and precipitation over the warmest annual quarter), …

Herbicide And Additive Impacts On Bradyrhizobium Japonicum Growth In Solution, Joy Amajioyi, Thandiwe Nleya, Senthil Subramanian, Sharon A. Clay

Agronomy, Horticulture and Plant Science Faculty Publications

Plant biostimulants include beneficial fungi and bacteria, and are often applied to foliage to improve crop growth, yield, and/or crop quality. Crop improvements due to biostimulant addition may be modest; therefore, solo applications may not be economical or climate smart. However, biostimulants combined with other postemergence treatments, such as herbicides, may provide an alternative application method, if mixtures do not harm the living organism(s). The growth of Bradyrhizobium japonicum, as a biostimulant surrogate, was assessed in solutions of glyphosate [N-(phosphonomethyl)glycine] and dicamba (3,6-dichloro-2-methoxybenzoic acid), with and without common spray additives (ammonium sulfate [AMS] and nonionic surfactant) in laboratory studies over …

Summaries Of Arkansas Cotton Research 2022, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

The current economic environment continues to drive the need to produce record or near-record yields to be profitable. Price volatility in 2022 added another level of difficulty in the quest for being profitable. The cotton market saw significant movement after reaching a $1.5802 per pound high in May 2022, cotton prices corrected and more than halved in value at the October 2022 low, and then consolidated around the 80 cents per pound level in December 2022 (Cotton is Consolidating). Great uncertainties still exist for the upcoming season, most of which are outside of our control. These include, but are not …