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Agronomy and Crop Sciences

Theses and Dissertations--Plant and Soil Sciences

Alternative splicing

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Genetic And Functional Analysis Of Host Genes Involved In Pathogenic And Symbiotic Legume-Microbe Interactions, Fang Tang Jan 2015

Genetic And Functional Analysis Of Host Genes Involved In Pathogenic And Symbiotic Legume-Microbe Interactions, Fang Tang

Theses and Dissertations--Plant and Soil Sciences

Legumes form symbiotic and pathogenic interactions with microbes. Understanding the molecular mechanisms underlying the legume-microbe interactions would help us to improve crop production in a sustainable manner. This thesis covers two independent research projects. The first project was to study the role of alternative splicing in RCT1-mediated disease resistance. RCT1 is a TIR-NBS-LRR-type plant resistance (R) gene in Medicago truncatula that confers broad-spectrum resistance to Colletotrichum trifolii, a fungal pathogen that causes anthracnose disease in Medicago. RCT1 undergoes alternative splicing at both coding and 3'-untranslated regions, thereby producing multiple transcript variants in its expression profile. …