Plant Sciences Commons^™

Nested Association Mapping Of Stem Rust Resistance In Wheat Using Genotyping By Sequencing, Prabin Bajgain, Matthew N. Rouse, Toi J. Tsilo, Godwin K. Macharia, Sridhar Bhavani, Yue Jin, James A. Anderson

Department of Agronomy Faculty Publications

We combined the recently developed genotyping by sequencing (GBS) method with joint mapping (also known as nested association mapping) to dissect and understand the genetic architecture controlling stem rust resistance in wheat (Triticum aestivum). Ten stem rust resistant wheat varieties were crossed to the susceptible line LMPG-6 to generate F₆ recombinant inbred lines. The recombinant inbred line populations were phenotyped in Kenya, South Africa, and St. Paul, Minnesota, USA. By joint mapping of the 10 populations, we identified 59 minor and medium-effect QTL (explained phenotypic variance range of 1%- 20%) on 20 chromosomes that contributed towards adult plant resistance …