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Clamping Of Intracellular Ph In Neurons From Neonatal Rat Brainstem During Hypercapnia, Vivian C. Nanagas
Clamping Of Intracellular Ph In Neurons From Neonatal Rat Brainstem During Hypercapnia, Vivian C. Nanagas
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In this work, I have made attempts to clamp intracellular pH in the presence of hypercapnic acidosis (HA) in neurons from the locus coeruleus (LC) and nucleus of the solitary tract (NTS) in neonatal rat (ages P3 to P17) brainstem slices. Two approaches were used to minimize hypercapnia-induced ΔpHi: 1) an increase in intracellular buffering power with a high HEPES concentration using whole cell patching techniques in individual neurons, and 2) a weak acid diffusion technique that relies on an efflux of weak acid to counterbalance HA influx thereby clamping pHi in multiple neurons at once. pH …
The Role Of Nucleotide Signaling In The Regulation Of Icl,Swell In Human 1321n1 Astrocytoma Cells, Ian C. Wenker
The Role Of Nucleotide Signaling In The Regulation Of Icl,Swell In Human 1321n1 Astrocytoma Cells, Ian C. Wenker
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Swollen cells expel osmolytes to decrease volume in a process referred to as Regulatory Volume Decrease, or RVD. The plasma membrane current ICl,swell is ubiquitously expressed in mammalian cells and contributes significantly to RVD. In a variety of cell types, activation of ICl,swell is enhanced by nucleotide signaling. In this study, we used human astrocytoma cells to examine whether nucleotide signaling is necessary or sufficient for ICl,swell activation. Three clones of the 1321N1 human astrocytoma cell line were used for voltage clamp recordings. One clone was transfected with P2Y1 receptors, another with P2Y2 receptors and a third was devoid of …