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Full-Text Articles in Physiology
An Efficient Method For Generating Conditional Cell Lines For Cellular And Molecular Research, Mark Roth, Dr. Jonathan Alder
An Efficient Method For Generating Conditional Cell Lines For Cellular And Molecular Research, Mark Roth, Dr. Jonathan Alder
Journal of Undergraduate Research
Conditional cell lines are widely used throughout biochemical research. The basic concept is that you can delete a gene you are studying upon the addition of a drug, to cells in culture, and then use the knockout cells to study the genes effects. This process has been facilitated by the advent of the CRISPR/Cas9 system that allows a broad range of genome editing in living cells. Cas9 introduces a double strand break in DNA at a site specified by the CRISPR (guide RNA), and by changing the CRISPR sequence researchers can introduce these double strand breaks almost wherever they want. …