Physiology Commons^™

Using Dry Blood Spots To Evaluate Serum Cytokines And Chemokines In Humans Via Multiplex Technology, Eric A. Prado, Adam S. Venable, Andie L. Henning, Randall R. Williams, Brian K. Mcfarlin

International Journal of Exercise Science: Conference Proceedings

Introduction: Dried Blood Spot (DBS) analysis has been used routinely since 1963 for the assessment of metabolic diseases in neonates; however, recent efforts have focused on the refinement and validation of DBS for other patient populations. The purpose of this study was to adapt existing DBS methods to analyze 38 serum cytokines/chemokines in human subjects. Validation will be completed by comparing DBS to serum for a given analyte.

Methods: After providing informed consent, subjects (N=21) provided a finger-stick DBS or venous serum sample using standard technique. Subjects were apparently healthy, non-obese, and had no known disease. Finger-stick capillary blood samples …

Changes Of Cytokines In Mouse Skeletal Muscle And Plasma By A Single Bout Of Endurance Exercise., Minjung Lee, Noriaki Kawanishi, Koichi Yada, Yungli Hung, Tsubasa Mizokami, Hiroyuki Niihara, Katsuhiko Suzuki

International Journal of Exercise Science: Conference Proceedings

Introduction: Exercise influences some cytokine levels, which is considered the body’s reaction against endogenous stress. Previous human studies demonstrated that high intensity exercise contributes to a dramatic increase of plasma cytokines like Interleukin-6 (IL-6) and Interleukin-1 receptor antagonist (IL-1ra) immediately after exercise. Recently, it has been identified that skeletal muscle produces cytokines and peptides and they exert both paracrine and endocrine effects. However, the sequential changes of cytokine production according to exercise conditions such as intensity, types and working time are still unclear. In this study, we identified the sequential changes of cytokines in both skeletal muscle and plasma after …

Comparison Of Two Different Sprint Interval Training Work-To-Rest Ratios On Acute Metabolic And Inflammatory Responses, Christopher R. Harnish

Theses and Dissertations

High intensity exercise is believed to yield greater results on health and human performance than moderate intensity exercise. Extensive research indicates that not only do high-intensity interval training (HIT) and sprint interval training (SIT) produce significant improvements in cardiovascular fitness and disease, they may be more effective at improving long-term metabolic function, including insulin sensitivity (Si), by producing more mitochondria. Moreover, compliance rates for HIT and SIT participation are reported to be the same or better than traditional moderate intensity exercise. Because lack of time is often cited as major hindrance to exercise participation, SIT is also seen as a …