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Full-Text Articles in Pharmacology, Toxicology and Environmental Health
In Vitro Toxicological Evaluation Of Mesoporous Silica Microparticles Functionalised 1 With Carvacrol And Thymol, Cristina Fuentes, Ana Fuentes, Hugh Byrne, José Barat, María José Ruiz
In Vitro Toxicological Evaluation Of Mesoporous Silica Microparticles Functionalised 1 With Carvacrol And Thymol, Cristina Fuentes, Ana Fuentes, Hugh Byrne, José Barat, María José Ruiz
Articles
The cytotoxicity of carvacrol- and thymol- functionalised mesoporous silica microparticles (MCM-41) was assessed in the human hepatocarcinoma cell line (HepG2). Cell viability, lactate dehydrogenase (LDH) activity, reactive oxygen species (ROS) production, mitochondrial membrane potential (ΔΨm), lipid peroxidation (LPO) and apoptosis/necrosis analysis were used as endpoints. Results showed that both materials induced cytotoxicity in a time and concentration-dependent manner, being more cytotoxic than free essential oil components and bare MCM-41. This effect was caused by the cell-particle interactions and not from degradation products released to the culture media, as demonstrated in the extract dilution assays. LDH release was seen to be …
Cytotoxic Effect Of Crude Vitex Glabrata Fruit Extracts On Human Cancer Cell Lines, Saraphorn Intarasam, Vorranuch Keerathinant, Mesayamas Kongsema, Kantimanee Pradermwong
Cytotoxic Effect Of Crude Vitex Glabrata Fruit Extracts On Human Cancer Cell Lines, Saraphorn Intarasam, Vorranuch Keerathinant, Mesayamas Kongsema, Kantimanee Pradermwong
The Thai Journal of Pharmaceutical Sciences
Vitex glabrata (VG) or Khai-noa fruit extracts were prepared from three methods; squeezing extract (SE), maceration extract (ME), and ethanol extraction (EE) to evaluate their effects in vitro. By the (1,1-diphenyl-2-picrylhydrazyl) scavenging assay, all extracts revealed very weak antioxidant. Results of (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a tetrazole) assay showed the increase in cell viability of SE at 100 and 500 μg/ml on TK6 cells after 24 and 48 h treatments (P < 0.01) without affecting Caco-2 cells. Conversely, a dose- and time-dependent cytotoxicity of all extracts revealed at 1000-2000 μg/ml on both cells (P < 0.01). SE showed cytotoxicity on PC-3 cells and anticancer activity when confirmed by clonogenic assay in a dose-dependent manner. SE also showed cytotoxicity on HT-29 cells after 24 h treatment but the cells could recurrent in the clonogenic assay. No effect was observed on MCF-7 cells after SE alone, SE co-treated with MMC treatment, and in the clonogenic assay. Moreover, HT-29 cells revealed a dose-dependent decrease in viability when treated with SE co-treated with MMC by 24 h. Hence, SE can selectively promote cell viability on TK6 and anticancer activity on PC-3. These results suggest that the different potential of SE depends on different cell lines.
Fluid Properties Of Solvents And Oils Used In In Situ Forming Microparticles, Sai Myo Thu Rein, Torsak Intaraphairot
Fluid Properties Of Solvents And Oils Used In In Situ Forming Microparticles, Sai Myo Thu Rein, Torsak Intaraphairot
The Thai Journal of Pharmaceutical Sciences
In situ forming microparticle (ISM) has been recently used as the injectable drug delivery system prepared from polymeric non-aqueous phase in oil emulsion. Solvents and oils used in ISM including N-methyl pyrrolidone (NMP), 2-pyrrolidone (PYR), dimethyl sulfoxide (DMSO), glycofurol, triacetin, olive oil, camellia oil and isopropyl myristate (IPM) were evaluated for pH, density, viscosity, flow time, surface tension, contact angle, color distribution, injectability, antimicrobial activities and cytotoxicity. The most viscous camellia oil and olive oil showed the highest flow time with lowest color diffusion and lower injectability; thus, they were suitable for performing as external phase of ISM to prolong …