Pharmacology, Toxicology and Environmental Health Commons^™

Alterations In Rat Brain Norepinephrine And Dopamine Levels And Synthesis Rates In Response To Five Neurotoxic Chemicals: Acrylamide, 2,5-Hexanedione, Tri-O-Tolyl Phosphate, Leptophos, And Methyl Mercuric Chloride, Charles N. Aldous

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Acrylamide, 2,5-hexanedione, Tri-o-tolyl phosphate and leptophos belong to three fundamentally different chemical classes but all four chemicals cause central-peripheral distal axonopathy. Some of these compounds have been shown to alter brain steady state levels of neurotransmitters or to inhibit the activities of adenosine triphosphatases which are involved in the uptake and storage of biogenic amines. Tests were performed to determine alterations in steady state levels of rat brain norepinephrine and dopamine in response to doses of the above chemicals and of the central nervous system toxin, methyl mercuric chloride sufficient to cause ataxia. Catecholamine synthesis rate constant estimations were performed. …

Inhibition Of Synaptosomal Biogenic Amine Transport By A Diverse Group Of Neurotoxic Chemicals, William M. Bracken

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Synaptosomal membrane functions were monitored, after in vitro exposure to select environmental pollutants, in synaptosomal preparations originating from rat cerebral cortices. The uptake of NE and 5HT into the synaptosomes was monitored as was the K⁺-dependent phosphate activity of the membrane. CH₃HgCl, Hg(NO₃)₂, CdCl₂, diisopropylfluorophosphate (DFP), paraoxon, acrylamide and Kepone were the test chemicals whose effects were studied.

CH₃HgCl, Hg(NO₃)₂ and Kepone had the greatest inhibitory effects on NE and 5HT uptake. The concentrations producing 50 percent inhibition (IC₅₀) were 1.4 x 10 …