Pharmacology, Toxicology and Environmental Health Commons^™

The Covalent Interaction Of Hepatic Metabolites Of The Insecticide Chlordane With Cellular Macromolecules In The Rat And Mouse In Vitro, Alan A. Brimfield

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This investigation addressed several aspects of the covalent interaction of metabolites of the insecticide chlordane with cellular macromolecules in vitro. Microsomal preparations from the liver of mice and rats were used and covalent binding to microsomal protein and RNA or to added calf thymus DNA was studied. Pure ¹⁴C-labelled cis- and trans-chlordane isomers as well as an isomeric mixture (¹⁴C-cis-chlordane plus ¹⁴C-trans-chlordane 3:1, w:w) were used as substrates for the in vitro system. Biochemical parameters investigated included inhibition of microsomal mixed-function oxidase and epoxide hydratase plus the induction of these enzymes by pretreatment with chlordane …

A Rapid And Specific Gas Chromatographic Analysis For Cysteine-S-Sulfonate To Determine The Distribution Of Sulfite In Mammalian Plasma, Joseph Don Debethizy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

It has been shown in previous studies that when sulfite is absorbed by rabbits via either inhalation of SO₂ or oral exposure to sulfite, the hydrated form, bisulfite, interacts with plasma disulfides where it is suspected to be in the form, cysteine-S-sulfonate. A rapid and specific gas chromatographic analysis procedure for cysteine-S-sulfonate has been developed to better study the distribution of sulfite in biological systems. Sulfonated proteins are enzymatically hydrolyzed to ensure stability of the acid labile S-sulfonate disulfide. The hydrolysate is then applied to a 6 cm cation-exchange column and eluted with 0.1 N HCl which elutes the …

Central Nervous System Toxicity Of Alpha-Chaconine In Rats, Charles Newell Aldous Iii

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Effects of alpha-chaconine were examined in the central nervous system on the basis of electrophysiological tests and by assaying for changes in the levels of several known neurotransmitters of the brain. The possibility that brain acetylcholine levels might increase corresponding to acetylcholinesterase inhibition in treated rats was investigated where an increase would suggest that the anti-acetylcholinesterase activity of alpha-chaconine was physiologically significant.

Various doses of alpha-chaconine were given to rats prepared for physiological tests. Some symptoms were observed at relatively low doses (8 to 10 milligrams per kilogram). These included constriction of abdominal muscles, respiratory impairment, and sedation. At the …

The Toxicity, Metabolism And Distribution Of Carbaryl In Three Species Of Labops With And Without Piperonyl Butoxide Treatment (Hemiptera:Miridae), Deifalla H. Osman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Carbaryl toxicity, metabolism, and distribution in adults of three species of grass bugs from the genus Labops were studied in relation to species, sex, and treatment with piperonyl butoxide. LC₅₀ values for 8 hour exposure periods ranged from 0.02-0.14, 0.03-0.3, and 0.2-0.7 μg carbaryl/vial for L. utahensis, L. hirtus, and L. hesperius respectively. The males were more susceptible to carbaryl than females. Males of L. utahensis were more susceptible than L. hirtus and L. hesperius respectively.

The synergist difference values (LC₅₀ of carbaryl alone - LC₅₀ values of carbaryl after piperonyl butoxide treatment) were measured. …