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Full-Text Articles in Pharmacology, Toxicology and Environmental Health
The Effect Of Red Maple Leaf Toxicosis On Reduced Glutathione Levels In Equine Erythrocytes In Vitro, Madeline A. Rohl
The Effect Of Red Maple Leaf Toxicosis On Reduced Glutathione Levels In Equine Erythrocytes In Vitro, Madeline A. Rohl
Undergraduate Honors Thesis Projects
Red maple leaf toxicosis is an equine blood disorder resulting from the consumption of wilted red maple (Acer rubrum L.) leaves by horses. Compounds within the leaves of red maple have oxidative effects on equine erythrocytes and can cause hemolysis of erythrocytes, the conversion of hemoglobin to methemoglobin, and the production of Heinz bodies. Reduced glutathione is important in the protection of equine erythrocytes from these oxidative events; however, in the presence of red maple toxin, glutathione is rapidly oxidized and is unavailable. The objective of this study is to determine whether the presence of vitamin C alters levels …
Pyocyanin-Induced Toxicity In A549 Respiratory Cells Is Causally Linked To Oxidative Stress, Lee Gloyne, Gary Grant, Anthony Perkins, Katie Powell, Catherine Mcdermott, Peter Johnson, Gregory Anderson, Milton Kiefel, Shailendra Anoopkumar-Dukie
Pyocyanin-Induced Toxicity In A549 Respiratory Cells Is Causally Linked To Oxidative Stress, Lee Gloyne, Gary Grant, Anthony Perkins, Katie Powell, Catherine Mcdermott, Peter Johnson, Gregory Anderson, Milton Kiefel, Shailendra Anoopkumar-Dukie
Catherine M. McDermott
Pyocyanin, a virulence factor produced by Pseudomonas aeruginosa, has many damaging effects on mammalian cells. Several lines of evidence suggest that this damage is primarily mediated by its ability to generate ROS and deplete host antioxidant defence mechanisms. However, a causal role for oxidative stress has not yet been demonstrated conclusively. Parallel measures of ROS production, antioxidant levels and cytotoxicity provide convincing evidence that pyocyanin-induced cytotoxicity in A549 respiratory cells is mediated by acute ROS production and subsequent oxidative stress. Pyocyanin increased ROS levels in A549 cells as measured by the fluorescent H2O2 probes Amplex Red and DCFH-DA. These effects …